In Vitro α-Amylase and Protein Glycation Inhibitory Activity of the Aqueous Extract of Flueggea leucopyrus Willd by Vishmi Sachindra Thrikawala, Srianthie A. Deraniyagala, Chamira Dilanka Fernando, Dinusha Nishani Udukala

“…This study shows that Flueggea leucopyrus Willd not only inhibits α-amylase enzyme, which is known to break starch to glucose, but also inhibits the formation of AGEs, which occur due to chronic hyperglycemia that leads to the onset of diabetic complications.…”
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Occurrence of Bordetella Infection in Pigs in Northern India by Sandeep Kumar, Bhoj R. Singh, Monika Bhardwaj, Vidya Singh

“…In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs. …”
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Efficient Production of Fuel Ethanol via the Simultaneous Use of Distillery Stillage Biomass and Beet Molasses by Katarzyna Osmolak, Dawid Mikulski, Grzegorz Kłosowski

“…The maximum glucose concentration of about 12 g/L (hydrolysis efficiency about 35.5%) was achieved even with the lowest enzyme dose, i.e., 7.5 FPU per gram of biomass. The yeast also showed high fermentation activity in media prepared from stillage biomass and molasses, producing about 50 g/L of ethanol regardless of the fermentation strategy used. …”
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ATP-Binding Pocket-Targeted Suppression of Src and Syk by Luteolin Contributes to Its Anti-Inflammatory Action by Jeong-Oog Lee, Deok Jeong, Mi-Yeon Kim, Jae Youl Cho

“…In the present study, the anti-inflammatory mode of action in activated macrophages of luteolin from Artemisia asiatica was examined by employing immunoblotting analysis, a luciferase reporter gene assay, enzyme assays, and an overexpression strategy. Luteolin dose-dependently inhibited the secretion of nitric oxide (NO) and prostaglandin E2 (PGE2) and diminished the levels of mRNA transcripts of inducible NO synthase (iNOS), tumor necrosis factor- (TNF-) α, and cyclooxygenase-2 (COX-2) in lipopolysaccharide- (LPS-) and pam3CSK-treated macrophage-like RAW264.7 cells without displaying cytotoxicity. …”
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Plasminogen Activator Inhibitor-1 Level Correlates with Lipoprotein Subfractions in Obese Nondiabetic Subjects by Sándor Somodi, Ildikó Seres, Hajnalka Lőrincz, Mariann Harangi, Péter Fülöp, György Paragh

“…Plasma PAI-1, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and myeloperoxidase (MPO) concentrations were determined with enzyme-linked immunosorbent assay (ELISA), while serum paraoxonase-1 (PON1) activities were measured by spectrophotometry. …”
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Recent advances in genetics of aggressive behavior by J. D. Davydova, S. S. Litvinov, R. F. Enikeeva, S. B.  Malykh, E. K. Khusnutdinova

“…The most studied candidate genes for aggressive behavior are serotonergic (TPH1, TPH2, HTR2A, SLC6A4) and dopaminergic (DRD4, SLC6A3) system genes, as well as the serotonin or catecholamine metabolizing enzyme genes (COMT, MAOA). In addition, there is evidence that the hypothalamic-pituitary system genes (OXT, OXTR, AVPR1A, AVPR1B), the sex hormone receptors genes (ER1, AR), neurotrophin (BDNF) and neuronal apoptosis genes (CASP3, BAX) may also be involved in development of aggressive behavior. …”
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Occurrence and Exposure Assessment of Aflatoxin B1 in Omena (Rastrineobola argentea) from Kenya by Esther Marijani, Harrison Charo-Karisa, Emmanuel Kigadye, Sheila Okoth

“…Aflatoxin levels in Omena were determined using the enzyme-linked immunosorbent assay (ELISA). Omena intended for fish feed production was most contaminated with a mean concentration of 46.93 μg·kg−1 (2.24–115.23 μg·kg−1) compared with Omena intended for human consumption (mean = 19.42 μg·kg−1, range = 2.01–49.30 μg·kg−1). …”
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Comparative Analysis of Genetic Differences and Probiotic Characteristics of Human Gut-Dwelling Ligilactobacillus ruminis in Kashi, Xinjiang by YIN Tong, AN Meiling, LIANG Zhixuan, LU Yanzhuan, SUN Hailong, NI Yongqing

“…The carbohydrate-active enzyme database (CAZy) annotated a large number of genes involved in polysaccharide substrate metabolism, such as the glycoside hydrolase (GH)1 and GH13 gene families. …”
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Clinical and Morphological Manifestations of Gastritis and Serum Cytokine Levels in Schoolchildren with Familial History of Gastric Cancer by T. V. Polivanova, E. V. Kasparov, V. A. Vshivkov

“…Serum cytokine levels (IL-2, IL-4, IL-8, IL-18, IL-1β, IFN-α, TNM-α) were obtained in enzyme immunoassays (ELISA).Results. Schoolchildren with gastritis and familial history of gastric cancer revealed a higher 59.8% rate of dyspeptic complaints vs. 40.8% in negative history (p = 0.001), as well as complaints of weekly heartburn in 14.2 and 8.3% cas-es (p = 0.019), respectively. …”
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The relationship of growth differentiation factor 15 with some biochemical parameters in cardiovascular disease patients in Babylon City by Hiba Resheed Behayaa, Zinah Abbass Ali, Seenaa Badr Mohammed, Suhayr A. Alqaysi

“…The age ranged between 41 and 73 years for patients and control was considered for this study. Enzyme-linked immunosorbent assay technique was used for GDF15 estimation. …”
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Toxicological Evaluation and Protective Effects of Ethanolic Leaf Extract of Cassia spectabilis DC on Liver and Kidney Function of Plasmodium berghei-Infected Mice by Wiwied Ekasari, Anisah Mahardiani, Nindya T. Putri, Tutik S. Wahyuni, Heny Arwati

“…Regarding hepatomegaly, further plasma levels of hepatic enzyme were analyzed, as well as histopathological observation of the liver to determine the effect of the extract on liver. …”
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Unravelling a mechanistic link between mitophagy defect, mitochondrial malfunction, and apoptotic neurodegeneration in Mucopolysaccharidosis VII by Nishan Mandal, Apurba Das, Rupak Datta

“…Cognitive disability and neurodegeneration are prominent symptoms of Mucopolysaccharidosis VII (MPS VII), a lysosomal storage disorder caused by β-glucuronidase enzyme deficiency. Yet, the mechanism of neurodegeneration in MPS VII remains unclear thereby limiting the scope of targeted therapy. …”
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In Silico Study of Coumarins: Wedelolactone as a Potential Inhibitor of the Spike Protein of the SARS-CoV-2 Variants by Saurav Katuwal, Siddha Raj Upadhyaya, Rishab Marahatha, Asmita Shrestha, Bishnu P. Regmi, Karan Khadayat, Saroj Basnet, Ram Chandra Basnyat, Niranjan Parajuli

“…The SARS-CoV-2 spike (S) protein is an important target protein as it mediates the entry of the virus inside the host cells, which is initiated by the binding of the receptor-binding domain (RBD) to its cognate receptor, angiotensin-converting enzyme 2 (ACE-2). Herein, the inhibition potential of several naturally occurring coumarins was investigated against the spike proteins of SARS-CoV-2 variants using computational approaches. …”
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The effect of blood transfusion on serum hepcidin levels in chronically transfused patients of β-thalassemia major: An observational study in a tertiary care centre in Western Maha... by Sujay Bhowmik, Amit Kumar Biswas, Ajay Kumar Baranwal, Amit Ajay Pawar, Ujjwal Dimri

“…Blood samples, collected pretransfusion and 7 days posttransfusion, were evaluated for hemoglobin, serum ferritin, and serum hepcidin using enzyme immunoassay. STATISTICAL ANALYSIS: Data were statistically analyzed through SPSS software and P < 0.05 is considered statically significant. …”
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Concentration of Asprosin Associated with Poor Control of Type 2 Diabetes Mellitus in Diyala Province by Alaa Abed Mahmoud, Walaa Ismael Jassim, Ahmed Abdul Hussein Mohsin, Ahmed M. Senan

“…Asprosin level in serum was assessed by enzyme-linked immunosorbent assay (ELISA) technique for 110 Iraqi patients with type 2 diabetes mellitus(T2DM), as well as 70 individual healthy controls. …”
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The Association between Hypertension and Dementia in the Elderly by Michiya Igase, Katsuhiko Kohara, Tetsuro Miki

“…The PROGRESS study showed that the use of angiotensin-converting enzyme inhibitors (ACEIs), with or without diuretics, resulted in decrease incidence of stroke-related dementia (risk reduction 19%), but dementia without stroke was not reduced. …”
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Presence of Serum Antinuclear Antibodies Does Not Impact Outcomes in HBV-Related Acute-on-Chronic Liver Failure by Lin Lin, Bin Lin, Qing Lan, Longgen Liu, Jianchun Lu, Xiujun Zhang, Shuqin Zheng, Yuan Xue

“…Serum concentrations of ANA were detected using the enzyme-linked immunosorbent assay kit. Multiple nuclear dots, rim-like, and centromere patterns of ANA were detected using indirect immunofluorescence assay on HEp-2 cells. …”
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Mini meta-analysis of anticholinesterase actions of atorvastatin, simvastatin and rosuvastatin, and in silico identification of their protein targets in Mus musculus by Fouad Kasim Mohammad, Rawnaq Faris Al-Shalchi

“…Dyslipidemic statins reduce blood and brain cholinesterase (ChE) activities in mice, with scarce information on other protein/enzyme targets. The study aims at conducting a mini meta-analysis on in vivo and in vitro adverse anti-ChE effects of atorvastatin, simvastatin and rosuvastatin in mice, and using the SwissPrediction to identify in silico body target proteins. …”
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Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study by Bruno Märkl, Jochen Hardt, Simon Franz, Tina Schaller, Gerhard Schenkirsch, Bernadette Kriening, Reinhard Hoffmann, Stefan Rüth

“…Tissue samples were analyzed for uPA and PAI-1 using a commercially available Enzyme-Linked Immunosorbent Assay (ELISA). Tumor budding was analyzed on cytokeratin-stained slides. …”
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Brain-Specific Increase in Leukotriene Signaling Accompanies Chronic Neuroinflammation and Cognitive Impairment in a Model of Gulf War Illness by Sahithi Attaluri, Raghavendra Upadhya, Maheedhar Kodali, Leelavathi N. Madhu, Dinesh Upadhya, Bing Shuai, Ashok K. Shetty

“…The concentration of cysteinyl LTs (CysLTs), LTB4, and 5-Lipoxygenase (5-LOX), the synthesizing enzyme of LTs, were evaluated. CysLT and LTB4 concentrations were elevated in the hippocampus and the cerebral cortex, along with enhanced 5-LOX expression in neurons and microglia. …”
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