A Case of Alport Syndrome with Posttransplant Antiglomerular Basement Membrane Disease despite Negative Antiglomerular Basement Membrane Antibodies by EIA Treated with Plasmapheres... by Sumiko I. Armstead, Thomas Hellmark, Jorgen Wieslander, Xin J. Zhou, Ramesh Saxena, Nilum Rajora

“…The serum anti-GBM IgG was negative by enzyme immunoassay (EIA). On biopsy, he had crescentic glomerulonephritis with linear GBM fixation of IgG. …”
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Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models by Alexander I. Borzilov, Nikolay V. Volozhantsev, Olga V. Korobova, Lyubov V. Kolupaeva, Evgenia S. Pereskokova, Tatiana I. Kombarova, Mikhail M. Shneider, Konstantin A. Miroshnikov, Ivan A. Dyatlov, Anastasia V. Popova

“…In the case of delayed treatment, mouse survival decreased to 70% when injected with the phage and to 40% when treated with the recombinant enzyme. In the mouse burn skin infection model, the number of <i>A. baumannii</i> cells on the surface of the wound and in the deep layers of the skin decreased by several-fold after treatment with both the K9-specific phage and the recombinant depolymerase. …”
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The Effect of Lipids on the Structure and Function of Egg Proteins in Response to Pasteurization by Hao Yang, Qiang Shi, Zhongliang Wang, Xiao Chen, Fangfang Min, Xuanyi Meng, Ping Tong, Yong Wu, Hongbing Chen

“…The protein structural changes were analyzed using polypropylene gel electrophoresis, circular dichroism, ultraviolet absorption spectra, and exogenous fluorescence spectra, and the functional properties were assessed through solubility measurements and particle size analysis, while protein antigenicity was evaluated using an enzyme-linked immunosorbent assay. All the results revealed that oleic acid had the most significant effect on proteins’ secondary and tertiary structures, particularly affecting ovalbumin and ovotransferrin. …”
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Novel insight into the formation and inhibition mechanism of dipeptidyl peptidase-Ⅳ inhibitory peptides from fermented mandarin fish (Chouguiyu) by Daqiao Yang, Chunsheng Li, Laihao Li, Xianqing Yang, Shengjun Chen, Yanyan Wu, Yang Feng

“…The inhibition mainly resulted from the hydrogen bond and salt bridge between D4IPs and DPP-IV enzyme. This study provides important information on the proteases and related microbial strains to directionally prepare D4IPs in Chouguiyu.…”
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A meta-analysis on plant growth and heavy metals uptake with the application of 2,4-epibrassinolide in contaminated soils by Kuiju Niu, Hong Xiao, Yong Wang, Ting Cui, Chunxu Zhao

“…EBR application enhanced photosynthesis and the mitigation of oxidative damage by significantly boosting antioxidant enzyme activity, non-enzymatic antioxidants, and metabolites. …”
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The Decreased Treg Cells Number Associated with Retinal Lesion Size in Ocular Toxoplasmosis by Ovi Sofia, Muna Amalia, Herryanto Thomassawa, Loeki Enggar Fitri, Seskoati Prayitnaningsih, Hani Susianti

“…Isolation of peripheral blood mononuclear cells (PBMC) was performed to measure the number of Treg cells using flow cytometry and interleukin-27 levels were assessed using the Sandwich enzyme-linked immunosorbent assay (ELISA) technique. …”
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Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids by Anna Z. Szóstek, Antonio M. Galvão, Takuo Hojo, Kiyoshi Okuda, Dariusz J. Skarzynski

“…Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method. Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells. …”
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Role of cytokines and nitric oxide in the induction of tuberculostatic macrophage functions by Vera L. Petricevich, Rosely C. B. Alves

“…Tumour necrosis factor (TNF) activity was assayed by measuring its cytotoxic activity on L-929 cells, interleukin-6 (IL-6) and interferon γ (IFN-γ) were assayed by enzyme-linked immunosorbent assay (ELISA), whereas NO levels were detected by Griess colorimetric reactions in the culture supernatant of macrophages incubated with IFN-γ , TNF or NO and subsequently exposed to either BCG-I or BCG-S. …”
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Inhibitory Potential of Five Traditionally Used Native Antidiabetic Medicinal Plants on α-Amylase, α-Glucosidase, Glucose Entrapment, and Amylolysis Kinetics In Vitro by Carene M. N. Picot, A. Hussein Subratty, M. Fawzi Mahomoodally

“…EL, EO, AM, and SL extracts (5000 μg/mL) were found to significantly (P<0.05) inhibit α-glucosidase (between 87.41±3.31 and 96.87±1.37% inhibition). Enzyme kinetic studies showed an uncompetitive and mixed type of inhibition. …”
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Safety and Tolerability of Initiating Maximum-Dose Sacubitril-Valsartan in Patients on Target Dose Renin-Angiotensin System Inhibitors by Helena Norberg, Ellinor Bergdahl, Krister Lindmark

“…Guidelines recommend initiating half-dose sacubitril-valsartan before up-titration even to patients already on target dose angiotensin-converting enzyme (ACE) inhibitors or angiotensin receptor blockers (ARB). …”
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The Expression of miR-192 and Its Significance in Diabetic Nephropathy Patients with Different Urine Albumin Creatinine Ratio by Xiaoyu Ma, Canlu Lu, Chuan Lv, Can Wu, Qiuyue Wang

“…The serum miR-192 levels were detected by Real-Time PCR and transforming growth factor-β1 (TGF-β1) and fibronectin (FN) were detected by enzyme-linked immunosorbent assay. The relationships among these parameters were analyzed by Pearson correlation analysis and multiple linear regression analysis. …”
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Insights into the mechanism of antiproliferative effects of primaquine-cinnamic acid conjugates on MCF-7 cells by Mabeta Peace, Pavić Kristina, Zorc Branka

“…Further analysis to determine the effects of test compounds on caspase activity and on the levels of apoptosis proteins were undertaken using the enzyme-linked immunosorbent assay (ELISA). Haematoxylin and eosin staining revealed that compounds 1 and 3 induced morphological changes in MCF-7 cells characteristic of apoptosis, while 2-treated cells were in interphase. …”
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Evaluation of Irisin Level in Iraqi Patients with Type 2 Diabetes and Pre-Diabetes Status as a Predictive Factor by Hiba Mohammed Hatem, Kadhim Khudhair Ghudhaib

“…The current study included 138 subjects divided into three groups based on fasting blood glucose (FBG) and glycosylated hemoglobin criteria, including T2DM, 46 patients, pre-diabetes, 46 participants, and healthy subject groups, 46 persons) when they enrolled in the National Diabetes Center/ Al-Mustansiriya University. Enzyme-linked immunosorbent assays were used to detect the levels of irisin and insulin, and spectrophotometric techniques were used to determine the levels of FBG and lipid profile. …”
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Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments by José A. O'Daly, Humberto M. Spinetti, Joe Gleason, María B. Rodríguez

“…Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P<0.001), with a diagnostic value from 1.50 to 1.84. …”
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Molecular, Biochemical, and Clinical Characterization of Thirteen Patients with Glycogen Storage Disease 1a in Malaysia by Siti Aishah Abdul Wahab, Yusnita Yakob, Mohd Khairul Nizam Mohd Khalid, Noraishah Ali, Huey Yin Leong, Lock Hock Ngu

“…GSD1a is caused by a mutation in the G6PC gene encoding glucose-6-phosphatase (G6Pase); an enzyme that catalyses the hydrolysis of glucose-6-phosphate (G6P) to phosphate and glucose. …”
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Metabolic pathways of eicosanoids—derivatives of arachidonic acid and their significance in skin by Michał Biernacki, Elżbieta Skrzydlewska

“…Phospholipids are subject to continuous transformation, including enzymatic hydrolysis (with the participation of phospholipases A, C, and D) to free polyunsaturated fatty acids (PUFAs), which under the influence of cyclooxygenases (COX1/2), lipoxygenases (LOXs), and cytochrome P450 (CYPs P450) are metabolized to various classes of oxylipins, depending on the type of PUFA being metabolized and the enzyme acting. The most frequently analyzed oxylipins, especially in skin cells, are eicosanoids, which are derivatives of arachidonic acid (AA). …”
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Network Pharmacology Analysis and Experimental Validation to Explore the Anti-inflammatory Mechanism of Asiatic Acid on Alcoholic Steatohepatitis by Tao Jiang, Jinhong Xu, Yang Lu, Xiaojin Chen, Yongxia Li

“…In addition, in the cellular model, real-time reverse transcriptase-polymerase chain reaction (Real-Time RT-PCR) and enzyme-linked immunosorbent assay (ELISA) results showed that AA could alleviate alcohol-induced cellular inflammation, while western blot and immunofluorescence results showed that AA might alleviate alcohol-induced cellular inflammation by inhibiting the nuclear factor-κB (NF-κB) pathway. …”
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The Effects of Laurencia caspica Algae Extract on Hemato-Immunological Parameters, Antioxidant Defense, and Resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis... by Majid Khanzadeh, Babak Beikzadeh, Seyed Hossein Hoseinifar

“…In addition, feeding with L. caspica significantly increased the antioxidant enzyme activities on the 25th day (L. caspica 1% and 2% in peroxidase [POD] and superoxide dismutase [SOD] in all groups) and 50th day (catalase [CAT], SOD and L. caspica 1% and 2% in POD) in the spleen. …”
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Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy by Awais ALi, Abdelkarem Omneya, Kashif Adil

“…Total plasma glycosaminoglycans (GAGs) levels were analyzed through a manual enzyme-linked immunosorbent assay using a non-competitive sandwich technique. …”
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Association between Elevated Serum Tau Protein Level and Sepsis-Associated Encephalopathy in Patients with Severe Sepsis by Teng Zhao, Yan Xia, Dawei Wang, Li Pang

“…The serum tau protein level was measured using a commercial enzyme-linked immunosorbent assay. The association between the level of serum tau protein and SAE was assessed by multiple logistic regression analysis. …”
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