The Protective Effect of Naringenin on Airway Remodeling after Mycoplasma Pneumoniae Infection by Inhibiting Autophagy-Mediated Lung Inflammation and Fibrosis by Yan Lin, Dan Tan, Qianna Kan, Zhen Xiao, Zhiyan Jiang

“…Here, we studied 124 children with or without MPP and compared inflammatory cytokines and fibrinogen-related protein expression with enzyme-linked immunosorbent assay. We also employed a BALB/c mouse model of MPP and divided the mice into three groups: ctrl (normal control mice), MPP (MP-infected mice), and MPP + NRG (MP-infected mice treated with NRG). …”
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Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava by Dubravka Štajner, Boris M. Popović, Dušica Ćalić, Marijana Štajner

“…Here we report antioxidant enzyme activities of superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase, reduced glutathione quantity, flavonoids, soluble protein contents, quantities of malondialdehyde, and •OH radical presence in the investigated plant samples. …”
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The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway by Jiaying Huo, Yuezhang Ming, Huifeng Li, Anjun Li, Jiwen Zhao, Mingquan Huang, Weizheng Sun, Jihong Wu, Jinglin Zhang

“…The results showed that these peptides exerted protective effects by suppressing reactive oxygen species (ROS) generation, preventing malondialdehyde (MDA) formation, and upregulating cellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) in a dose-dependent manner. …”
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Seroprevalence of <i>Borrelia</i>, <i>Anaplasma</i>, <i>Bartonella</i>, <i>Toxoplasma</i>, <i>Mycoplasma</i>, <i>Yersinia</i>, and <i>Chlamydia</i> in Human Population from Eastern... by Angelina Wójcik-Fatla, Anna Sawczyn-Domańska, Anna Kloc, Joanna Krzowska-Firych, Jacek Sroka

“…Anti-<i>Borrelia burgdorferi</i> sensu lato (s.l.) antibodies were tested with ELISA and Western blot (WB) tests; the detection of anti-<i>Toxoplasma gondii</i> antibodies was performed using an enzyme linked fluorescence assay (ELFA). Antibodies to bartonellosis, anaplasmosis, and chlamydiosis were determined by indirect immunofluorescent test (IFA), whereas antibodies to yersiniosis and mycoplasmosis were confirmed in the ELISA test. …”
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Highly Sensitive In Vivo Imaging of Bacterial Infections with a Hydrophilicity‐Switching, Self‐Immobilizing, Near‐Infrared Fluorogenic β‐Lactamase Probe Enriched within Bacteria... by Fangfang Chen, Yuyao Li, Yan Peng, Yifan Zhu, Gao He, Zhengwei Zhang, Hexin Xie

“…On the other hand, the expression of the β‐lactam‐hydrolyzing enzyme (β‐lactamase), the major cause of bacterial resistance to β‐lactam antibiotics, provides a distinctive opportunity to visualize bacterial infection, evaluate the efficacy of existing antibiotics, screen for novel antibacterial agents, and optimize drug dosing regimens in live animals. …”
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Room temperature CRISPR diagnostics for low-resource settings by Eric A. Nalefski, Selma Sinan, Jason L. Cantera, Anastasia G. Kim, Remy M. Kooistra, Rafael E. Rivera, Jordyn P. Janshen, Sanchita Bhadra, Joshua D. Bishop, Andrew D. Ellington, Ilya J. Finklestein, Damian Madan

“…To mitigate these limitations, we identify the ortholog TsCas12a as a highly active nuclease at 25 °C and develop a single-protocol RPA-Cas12a detection reaction with this enzyme. A quantitative kinetic analysis reveals that fast nuclease activation is more critical than higher steady-state trans-cleavage activity for room temperature diagnostic applications. …”
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Liver GlucokinaseA456V Induces Potent Hypoglycemia without Dyslipidemia through a Paradoxical Induction of the Catalytic Subunit of Glucose-6-Phosphatase by Anna Vidal-Alabró, Alícia G. Gómez-Valadés, Andrés Méndez-Lucas, Jordi Llorens, Ramon Bartrons, Jordi Bermúdez, Jose C. Perales

“…Several GK mutations affect GKRP binding, resulting in permanent activation of the enzyme. We hypothesize that hepatic overexpression of a mutated form of GK, GKA456V, described in a patient with persistent hyperinsulinemic hypoglycemia of infancy (PHHI) and could provide a model to study the consequences of GK-GKRP deregulation in vivo. …”
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Anti-proteolytic regulation of KRAS by USP9X/NDRG3 in KRAS-driven cancer development by Han Koo, Kyung Chan Park, Hyun Ahm Sohn, Minho Kang, Dong Joon Kim, Zee-Yong Park, Sehoon Park, Sang Hyun Min, Seong-Hwan Park, Yeon-Mi You, Yohan Han, Bo-Kyung Kim, Chul-Ho Lee, Yeon-Soo Kim, Sang J. Chung, Young Il Yeom, Dong Chul Lee

“…Here, we report that KRAS expression is post-translationally up-regulated through deubiquitination when the scaffolding function of NDRG3 (N-Myc downstream-regulated gene 3) promotes specific interaction between KRAS and a deubiquitinating enzyme, USP9X. In KRAS-mutant cancer cells KRAS protein expression, downstream signaling, and cell growth are highly dependent on NDRG3. …”
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Ticlopidine in Its Prodrug Form Is a Selective Inhibitor of Human NTPDase1 by Joanna Lecka, Michel Fausther, Beat Künzli, Jean Sévigny

“…Ticlopidine (100 µM) completely inhibited the ATPase activity of NTPDase1 in situ as shown by enzyme histochemistry with human liver and pancreas sections. …”
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IgG, IgM, and IgA Antinuclear Antibodies in Discoid and Systemic Lupus Erythematosus Patients by Sheridan A. Jost, Lin-Chiang Tseng, Loderick A. Matthews, Rebecca Vasquez, Song Zhang, Kim B. Yancey, Benjamin F. Chong

“…In this cross-sectional study, sera from age-, gender-, and ethnic-matched SLE N=35, DLE N=23, and normal patients N=22 were tested for IgG, IgM, and IgA ANAs using enzyme-linked immunosorbent assays (ELISAs) and indirect immunofluorescence (IIF) with monkey esophagus as substrate. …”
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Difference in the Vitreal Protein Profiles of Patients with Proliferative Diabetic Retinopathy with and without Intravitreal Conbercept Injection by Chen Zou, Minjie Zhao, Jingjing Yu, Dandan Zhu, Yunzhi Wang, Xinping She, Yanan Hu, Zhi Zheng

“…Intravitreal levels of apolipoprotein A-II (APOA2) and ceruloplasmin (CP) were measured using enzyme-linked immunosorbent assays. Results. 307 proteins were expressed differentially between PDR and IVC groups, including 218 proteins downregulated in response to IVC. …”
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Effect of Astaxanthin on Immune Function and Myocardial Injury in Mice with Chronic Sleep Deprivation by FENG Biyun, LI Lingyan, LI Ming, ZHANG Jiayuan, QI Huanyang, CHEN Pingping, XU Guanghui

“…Immune function and myocardial injury were evaluated by measuring organ coefficients as well as using the carbon clearance test and commercial enzyme-linked immunosorbent assay (ELISA) kits. The targets of astaxanthin and related biological processes and pathways were analyzed by network pharmacology. …”
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Multigenerational effects of cultivating transgenic maize straw on earthworms: A combined laboratory and field experiment by Li Zhang, Wenjing Shen, Zhixiang Fang, Laipan Liu, Ruizong Jia, Jingang Liang, Biao Liu

“…The enzymatic activity assay results revealed no significant differences in superoxide dismutase (SOD) or catalase (CAT) enzyme activity between the GM and non-GM maize varieties. …”
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Construction of a multienzyme cascade reaction system and its application in D-tagatose biosynthesis by Xiaoxiao Zhang, Jie Chu, Yuanqiang Lv, Xuan Li, Aijiao Yin, Yanhua Huang

“…This multienzyme approach improved the conversion of the intermediate product D-glucose to D-tagatose by 3.84% compared with the dual-enzyme system. Thus, our study provides a theoretical basis and technical support for the industrial production of D-tagatose.…”
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Serum Osteoprotegerin Is a Potential Biomarker of Insulin Resistance in Chinese Postmenopausal Women with Prediabetes and Type 2 Diabetes by Peng Duan, Min Yang, Meilin Wei, Jia Liu, Ping Tu

“…Serum OPG levels were measured by enzyme-linked immunosorbent assay. The serum OPG concentration in NGR group, 151.00 ± 45.72 pg/mL, was significantly lower than that in IGR group (169.28 ± 64.91 pg/mL) (p=0.031) and T2DM group (183.20 ± 56.53 pg/mL) (p<0.01), respectively. …”
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Investigation of Anaplasma marginale Seroprevalence in a Traditionally Managed Large California Beef Herd by Thomas R. Tucker, Sharif S. Aly, John Maas, Josh S. Davy, Janet E. Foley

“…Serum samples were collected to determine Anaplasma marginale seroprevalence using a commercially available competitive enzyme-linked immunosorbent assay test kit. Repeat sampling was performed in seronegative animals to determine the incidence density rate from March through September (2013). …”
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CRAT downregulation promotes ovarian cancer progression by facilitating mitochondrial metabolism through decreasing the acetylation of PGC-1α by Zhen Zhang, Shuhua Zhao, Xiaohui Lv, Yan Gao, Qian Guo, Yanjie Ren, Yuanyuan He, Yihua Jin, Hong Yang, Shujuan Liu, Xiaohong Zhang

“…Carnitine acetyltransferase (CRAT) is a mitochondrial-localized enzyme catalyzing the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. …”
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Synovial Fluid Cell Proteomic Analysis Identifies Upregulation of Alpha-Taxilin Proteins in Rheumatoid Arthritis: A Potential Prognostic Marker by Ashish Sarkar, Shivani Sharma, Prachi Agnihotri, Tanmoy Sarkar, Pooja Kumari, Rajesh Malhotra, Barun Datta, Vijay Kumar, Sagarika Biswas

“…It has been validated in the synovium, synovial fluid (SF), SF cells, and plasma samples by Western blot, enzyme-linked immunosorbent assay (ELISA), fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and real-time PCR. …”
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Increasing the concentration of plasma molecules improves the biological activity of platelet-rich plasma for tissue regeneration by Mikel Sánchez, Jon Mercader Ruiz, Daniel Marijuán Pinel, Pello Sánchez, Nicolás Fiz, Jorge Guadilla, Juan Azofra, Maider Beitia, Diego Delgado

“…Different growth factor (GF) levels were measured using Enzyme Linked Immunosorbent Assay (ELISA), and in addition to the increase in intra-platelet GFs found in standard PRP (sPRP), BPCP also showed a higher concentration of plasmatic protein. …”
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Discovery of Anti-inflammatory Peptides from Channa argus Using Virtual Screening, Molecular Docking, and Cell Model by XIANG Huan, LU Meiming, CHEN Shengjun, HUANG Hui, HU Xiao, ZHAO Yongqiang, WEI Ya

“…Virtual hydrolysis was used to select the best enzyme for obtaining bioactive peptides from C. argus proteins. …”
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