Changes in wheat protein digestibility and allergenicity: Role of Pediococcus acidilactici XZ31 and yeast during dough fermentation by Wenhui Fu, Chen Chen, Chenglong Liu, Sha Tao, Wentong Xue

“…Protein digestibility were evaluated using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and enzyme-linked immunosorbent assay. The effect of protein on intestinal permeability was investigated by Caco-2 cell monolayers. …”
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Search for Anti-EA(D) Antibodies in Subjects with an “Isolated VCA IgG” Pattern by Massimo De Paschale, Debora Cagnin, Teresa Cerulli, Maria Teresa Manco, Carlo Agrappi, Paola Mirri, Arianna Gatti, Cristina Rescaldani, Pierangelo Clerici

“…However, one alternative is to use an enzyme-linked immunosorbent assay (ELISA) to detect anti-early antigen (EA) antibodies, which can be found in about 85% of subjects with acute Epstein-Barr virus (EBV) infections. …”
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High Level Aminoglycoside Resistance and Distribution of Aminoglycoside Resistant Genes among Clinical Isolates of Enterococcus Species in Chennai, India by Elango Padmasini, R. Padmaraj, S. Srivani Ramesh

“…Detection of aminoglycoside modifying enzyme encoding genes (AME) in enterococci was identified by multiplex PCR for aac(6′)-Ie-aph(2′′)-Ia; aph(2′′)-Ib; aph(2′′)-Ic; aph(2′′)-Id and aph(3′)-IIIa genes. 38.2% isolates carried aac(6′)-Ie-aph(2′′)-Ia gene and 40.4% isolates carried aph(3′)-IIIa gene. aph(2′′)-Ib; aph(2′′)-Ic; aph(2′′)-Id were not detected among our study isolates. aac(6′)-Ie-aph(2′′)-Ia and aph(3′)-IIIa genes were also observed in HLAR E. durans, E. avium, E. hirae, and E. gallinarum isolates. …”
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The association between salivary oxytocin, age, and puberty in children with and without OCD by Anna-Rosa Cecilie Mora-Jensen, Line Katrine Harder Clemmensen, Manja Gersholm Grønberg, Eli R. Lebowitz, Daniel S. Quintana, Niklas Rye Jørgensen, Charlotte Sewerin Larsen, Lasse Kristoffer Bak, Gitte Lund Christensen, Linea Pretzmann, Valdemar Uhre, Sofie Heidenheim Christensen, Camilla Uhre, Nicoline Løcke Jepsen Korsbjerg, Christine Lykke Thoustrup, Julie Hagstrøm, Melanie Ritter, Kerstin Jessica Plessen, Anne Katrine Pagsberg, Nicole Nadine Lønfeldt

“…Salivary oxytocin concentrations were measured in children and parents with enzyme-linked immunosorbent assay (ELISA). Statistical analyses were performed using frequentist and Bayesian approaches. …”
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Increased Kappa/Lambda Hybrid Antibody in Serum Is a Novel Biomarker Related to Disease Activity and Inflammation in Rheumatoid Arthritis by Lang Yi, Mingju Hao, Tian Lu, Guigao Lin, Lida Chen, Ming Gao, Gaowei Fan, Dong Zhang, Guojing Wang, Xin Yang, Yulong Li, Kuo Zhang, Rui Zhang, Yanxi Han, Lunan Wang, Jinming Li

“…Here, we developed a double-sandwich enzyme-linked immunosorbent assay system to quantify relative serum κ/λ hybrid antibody levels in RA patients, osteoarthritis (OA) patients, and healthy controls (HC) in order to assess their potential use as a serological biomarker of early disease and clinical activity and to preliminarily investigate their immunomodulatory roles in RA. …”
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Sustainable Protein Sources: Functional Analysis of <i>Tenebrio molitor</i> Hydrolysates and Attitudes of Consumers in Poland and Spain Toward Insect-Based Foods by Marcelina Maciejewska, Anna Dąbrowska, Marina Cano-Lamadrid

“…The study optimized the process of enzyme hydrolysis using serine protease from <i>Cucurbita ficifolia</i>, thereby enhancing DPPH scavenging capacity increased from 3.15 ± 0.53 to 8.17 ± 0.62 µM Trolox/mL and ABTS decolorization capacity increased from 4.29 ± 0.01 to 10.29 ± 0.01 µM Trolox/mL after 5 h of hydrolysis. …”
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Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells by Si-Zhe Li, Xin-Xing Jin, Xiao-Lei Ge, Ya-Gang Zuo, Hong-Zhong Jin

“…TSLP levels in sera and blister fluids were measured by enzyme-linked immunosorbent assay. The TSLP expression in the BP lesional skin was detected by immunohistochemical staining. …”
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Interferon-γ and Interleukin-10 Responses during Clinical Malaria Episodes in Infants Aged 0–2 Years Prenatally Exposed to Plasmodium falciparum: Tanzanian Birth Cohort by Boniphace Sylvester, Dinah B. Gasarasi, Said Aboud, Donath Tarimo, Siriel Masawe, Rose Mpembeni, Göte Swedberg

“…This prospective cohort study involved 215 infants aged 0-2 years born to mothers with or without placental malaria. Enzyme-linked immunosorbent assay (ELISA) was used to determine levels of IL-10 and IFN-γ in infants and detect IgM in cord blood. …”
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High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing by Junhao Kong, Xiufang Yang, Xiaobo Zuo, Xiaoqin Su, Bing Hu, Xinle Liang

“…The changes of aroma profiles and chemical constituents including theaflavins (TFs), thearubigins (TRs), theabrownines (TBs) and TRs/TFs ratio in converted tea brews under two-stage enzyme catalyzed fermentation and extraction conditions were further compared between different treatments, based on which the optimised conditions were determined. …”
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Manganese biofortification in grapevine by foliar spraying improves volatile profiles of Cabernet Sauvignon grapes and wine sensory traits by Ruihua Ren, Miaomiao Wang, Lijian Zhang, Fuxian Ren, Bowei Yang, Huangzhao Chen, Zhenwen Zhang, Qingqing Zeng

“…Manganese (Mn) is involved in plant metabolism as an enzyme cofactor. However, the role of Mn in the formation of volatile compounds in grapes has rarely been studied. …”
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Effect of Ozone Combined with 1-MCP on the Color and Quality of Nectarines during Postharvest Storage by Chen LIU, Yibin ZHANG, Xiaoyu JIA, Zhiyong ZHAO

“…Compared with 1-MCP group alone, 1-MCP group combined with ozone treatment had higher anthocyanin content, centaurin-3-O-glucoside content, centaurin-3-O-rutin content and enzyme activity related to anthocyanin metabolism at the later stage of storage. …”
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Acute Kidney Injury Secondary to Necrotizing Sarcoid Granulomatosis by Varun Mamidi, Manikantan Shekar, Jaiju James Chakola, Vamsi Krishna Makkena, Jayakumar Matcha

“…All the markers of tuberculosis were negative and angiotensin converting enzyme levels were elevated. Patient improved with 1 mg/kg/day oral steroid therapy and is on regular follow-up with minimal dose of steroids. …”
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Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency by Haitham Al-Madhagi, Abd Alraouf Tarabishi, Fatima Al-Zahra Hamsho, Zaher Tahan, Lama Alchab, Wassim Abdelwahed

“…The classic utilization of nanomaterials has now transformed to serve enzyme-like activities (nanozymes) that found different applications, particularly as antibacterial agents. …”
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Optimization of Enzymatic Hydrolysis Process of Morchella esculenta and Analysis of Volatile Flavor Compounds in Enzymatic Hydrolysate by Xiaofeng ZENG, Mi YAN, Zhixing GE, Lunjiang GAO, Xumin YIN, Yuan DIAO, Sang SHANG, Shunde ZENG

“…Results indications were as follows: enzyme addition amount of 5000 U/g, pH6.7, enzymolysis temperature at 44 ℃ for 2 h, the hydrolysis degree was 34.11% under the above mentioned conditions and a total of 23 volatile flavor compounds were identified by GC-MS analysis, among which 17 werein blank group and 21 in enzymatic group. …”
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The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood by Okko Savonius, Irmeli Roine, Saeed Alassiri, Taina Tervahartiala, Otto Helve, Josefina Fernández, Heikki Peltola, Timo Sorsa, Tuula Pelkonen

“…MMP-9 levels in the CSF were assessed by zymography, while MMP-8, MPO, and TIMP-1 concentrations were determined with immunofluorometric and enzyme-linked immunosorbent assays. Results. MPO correlated positively with MMP-8 (rho 0.496, P<0.001) and MMP-9 (rho 0.153, P=0.02) but negatively with TIMP-1 (rho -0.361, P<0.001). …”
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Structural basis for RNA-guided DNA degradation by Cas5-HNH/Cascade complex by Yanan Liu, Lin Wang, Qian Zhang, Pengyu Fu, Lingling Zhang, Ying Yu, Heng Zhang, Hongtao Zhu

“…Notably, we also find that divalent ions such as zinc, cobalt, and nickel down-regulate enzyme activity by destabilizing the Cascade complex. …”
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Effect of Intensive Blood Pressure Control on Cardiovascular Remodeling in Hypertensive Patients with Nephrosclerosis by Otelio Randall, John Kwagyan, Tamrat Retta, Kenneth Jamerson, Velvie Pogue, Keith Norris, Muluemebet Ketete, Shichen Xu, Tom Greene, Xuelei Wang, Lawrence Agodoa

“…Subjects were randomly assigned to usual MAP goal (102–107 mmHg) or a lower MAP goal (≤92 mmHg) and randomized to beta-blocker, angiotensin converting enzyme inhibitor, or calcium channel blocker. After covariate adjustment, a higher PP was associated with increased risk of CV outcome (RR = 1.28, CI = 1.11–1.47, P<0.01) and new LVH (RR = 1.26, CI = 1.04–1.54, P=0.02). …”
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Peptides: Emerging Candidates for the Prevention and Treatment of Skin Senescence: A Review by Andrada Pintea, Andrei Manea, Cezara Pintea, Robert-Alexandru Vlad, Magdalena Bîrsan, Paula Antonoaea, Emöke Margit Rédai, Adriana Ciurba

“…Based on their mechanism of action, they can be classified into signal peptides, carrier peptides, neurotransmitter inhibitor peptides, and enzyme inhibitor peptides. This review focuses on the main types of peptides and their application in the cosmetic field, underlining their main limitations. …”
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Ion suppression correction and normalization for non-targeted metabolomics by Iqbal Mahmud, Bo Wei, Lucas Veillon, Lin Tan, Sara Martinez, Bao Tran, Alexander Raskind, Felice de Jong, Yiwei Liu, Jibin Ding, Yun Xiong, Wai-kin Chan, Rehan Akbani, John N. Weinstein, Chris Beecher, Philip L. Lorenzi

“…To demonstrate a routine application of the Workflow, we employ the Workflow to study ovarian cancer cell response to the enzyme-drug L-asparaginase (ASNase). The IROA-normalized data reveal significant alterations in peptide metabolism, which have not been reported previously. …”
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TNF-α and Microalbuminuria in Patients with Type 2 Diabetes Mellitus by I.-Th. Lampropoulou, M. Stangou, A. Papagianni, T. Didangelos, F. Iliadis, G. Efstratiadis

“…Patients with microalbuminuria were older, with higher glycosylated hemoglobin levels (HbA1c) and they more frequently had diabetic retinopathy, neuropathy, and cardiovascular disease and were on treatment with angiotensin converting enzyme inhibitors (ACEi) and/or angiotensin receptor blockers (ARBs). …”
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