The Role of Mitochondrial NADPH-Dependent Isocitrate Dehydrogenase in Cancer Cells by Katarína Smolková, Petr Ježek

“…IDH2 acts in the forward Krebs cycle as an NADP+-consuming enzyme, providing NADPH for maintenance of the reduced glutathione and peroxiredoxin systems and for self-maintenance by reactivation of cystine-inactivated IDH2 by glutaredoxin 2. …”
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Remimazolam Combined with Andrographolide Improve Postoperative Cognitive Dysfunction in Rats after Cardiopulmonary Bypass through the AMPK/SIRT1 Signaling Pathway by Chong Chen, Lixia Lan, Kai Xu

“…Cognitive function was assessed using the Morris Water Maze test, and the concentrations of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) in serum were measured by enzyme-linked immunosorbent assay (ELISA). Apoptosis was evaluated using western blotting and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining assay. …”
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Carboxypeptidase B1 in dialyzed chronic kidney disease patients by Matej Stančík, Marián Grendár, Marián Mokáň

“…Abstract Carboxypeptidase B1 has long been known to be a pancreas-specific tissue enzyme. In this paper we focus on Carboxypeptidase B1 concentration measurement in dialyzed chronic kidney kidney disease patients and on the effect dialysis adequacy and regimen exert on its concentration. …”
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Polymorphism of the trehalase gene (TREH) in native populations of Siberia by B. A. Malyarchuk, M. V. Derenko

“…Here, in native populations of Siberia, we studied the polymorphism of the TREH gene, which encodes trehalase, an enzyme that cleaves trehalose. The analysis of exome polymorphism showed the presence of seven haplotypes of the TREH gene in the population. …”
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Evaluating the Factors Affecting COVID-19 Patients’ Mortality in Arak in 2020 by Hossein Mazaherpour, Masoumeh Soofian, Elham Farahani, Ahmad Saebbian, Mostafa Etebari, Sakineh Mazaherpour, Fatemeh Ashrafian, Amitis Ramezani

“…There was a significant relationship in terms of blood pressure, age, oxygen saturation, tachycardia, tachypnea, the interval between the onset of symptoms and hospitalization, diabetes mellitus, lung disease, cardiovascular disease, history of opium abuse, C-reactive protein, white blood cell, lymphocytes, hemoglobin, creatinine elevation, elevated liver enzyme, creatine phosphokinase, lactate dehydrogenase, ferritin, D-dimer, troponin, prothrombin time, international normalized ratio, intensive care unit admission days, arrhythmia, sepsis, acute respiratory distress syndrome, acute kidney injury (AKI), and the type of antiviral and antibiotic therapy between the two groups of patients. …”
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Prevalence and Risk Factors of Anaplasmosis in Simmental Cattle in the Peruvian Amazon by Hugo Frias, Luis Murga, William Bardales, Vanessa Frias, Segundo Melecio Portocarrero-Villegas, Tatiana Segura Portocarrero, Miguel Arista, José Américo Saucedo-Uriarte

“…In this research, the prevalence and risk factors of anaplasmosis in Simmental cattle raised in the Peruvian Amazon were evaluated. 266 blood samples were collected from bovines of different categories such as calves male, calves females, heifers <1.3 years, heifers >1.3 years, steers, bulls, and cows from the districts of Omia and Molinopampa. The enzyme-linked immunosorbent assay (ELISA) technique was used to detect antibodies against Anaplasma marginale. …”
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Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells by Mackenzie J. Dickson, I. Martin Sheldon, John J. Bromfield

“…Although treating granulosa cells with LPS in vitro decreases the expression of the estradiol synthesis enzyme CYP19A1 and reduces estradiol secretion, the molecular mechanisms are unclear. …”
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Positive Response of Hyoscyamus pusillus Callus Cultures to Exogenous Melatonin on Biochemical Traits and Secondary Metabolites under Drought Conditions by Shamil I. Neamah, Nisreen A. Jdayea

“…The decreased oxidative stress was associated with an increased antioxidant enzyme activity superoxide dismutase (SOD), and catalase (CAT) in the samples was treated by melatonin, which resulted in increased membrane stability index (MSI) and enhanced growth traits under the PEG treatment compared to the control. …”
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Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst by Joana T. Cunha, Aloia Romaní, Lucília Domingues

“…The selected yeast strain was engineered to express the oxidoreductase enzyme of HMF/Furfural from Cupriavidua basilensis strain HMF14, resulting in a 15-fold increase in the accumulation of oxidized derivatives such as 2,5-furandicarboxylic acid (FDCA). …”
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Silica Induces Changes in Cytosolic Free Calcium, Cytosolic pH, and Plasma Membrane Potential in Bovine Alveolar Macrophages by Attila Tárnok, Thomas Schlüter, Ingeborg Berg, Günther Gercken

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Expression and Secretion of CXCL-8 and CXCL-10 From <emph>Mycobacterium Bovis</emph> BCG-Infected Human Epithelial Cells: Role of IL-4

“…The levels of immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked immunosorbent assay. We found that, although <emph>M. bovis</emph> BCG induced gene expression of CXCL-8 and CXCL-10 in <emph>M. bovis</emph> BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. …”
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Association of Toll-Like Receptor 2 (TLR2) Expression in Placenta and Intrauterine Exposure to Hepatitis B Virus by Dian Utami, Maisuri T. Chalid, Rina Masadah, Rizalinda Sjahril, Andi Dwi Bahagia Febriani

“…Methods. We performed enzyme-linked immunoadsorbent assay of HBeAg, HBsAg titer, Anti-HBs, and Anti-HBc to 59 HBsAg-positive pregnant women and identified HBV DNA using nested PCR in their cord blood during delivery for evidence of exposure to HBV DNA. …”
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Mediators of intercellular interactions and histological signs of chronic viral liver disease by P. V. Koroy, A. V. Yagoda, G. I. Gilyazova, I. S. Mukhoramova

“…Scores of ICAM-1, VCAM-1, Е-selectin, L-selectin and the Р-selectin were determined by enzyme-linked immunoassay method and were compared to values of Knodell histological activity index and Desmet fibrosis score as well as to E.M. …”
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Impact of Power Ultrasound on Antihypertensive Activity, Functional Properties, and Thermal Stability of Rapeseed Protein Hydrolysates by Asif Wali, Haile Ma, Muhammad Shahnawaz, Khizar Hayat, Jian Xiaong, Li Jing

“…The effects of power ultrasound pretreatments on the degree of hydrolysis (DH), angiotensin-I-converting enzyme (ACE) inhibitory activity, amino acid composition, surface hydrophobicity, protein solubility, and thermal stability of ACE inhibition of rapeseed protein hydrolysates were evaluated. …”
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Freeze-Drying Microencapsulation of <i>Ruellia tuberosa</i> L. Extracts: A Comparative Study Using Different Polymers as Encapsulants by Firza Rajasa Gunawan, Siti Mariyah Ulfa, Anna Safitri

“…Alpha-amylase inhibition tests showed that microcapsules effectively inhibit the enzyme, with the coating combination performing best, followed by gum Arabic and then maltodextrin. …”
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Cognitive Status Correlates with CXCL10/IP-10 Levels in Parkinson’s Disease by Natália Pessoa Rocha, Paula Luciana Scalzo, Izabela Guimarães Barbosa, Mariana Soares Souza, Isabela Boechat Morato, Érica Leandro Marciano Vieira, Paulo Pereira Christo, Antônio Lúcio Teixeira, Helton José Reis

“…Peripheral blood was drawn and plasma levels of CCL2/MCP-1, CCL11/eotaxin, CCL24/eotaxin-2, and CXCL10/IP-10 were measured by enzyme-linked immunosorbent assay. PD patients and control individuals presented comparable plasma concentrations of all the evaluated chemokines. …”
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Allergic Contact Dermatitis Is Associated with Significant Oxidative Stress by S. Kaur, K. Zilmer, V. Leping, M. Zilmer

“…In 40 patients, interleukin- (IL-) 6, monocyte chemoattractant protein (MCP-1), and IL-10 levels were measured in sera with the Evidence Investigator Cytokine & Growth factors High-Sensitivity Array, total peroxide concentration (TPX) and total antioxidant capacity (TAC) by means of spectrophotometry, and the plasma concentrations of adiponectin and leptin by the quantitative sandwich enzyme immunoassay technique. Results. TNF-α level (P < 0.01) and TPX (P < 0.0001) were increased whilst IL-10 (P < 0.05) and TAC (P < 0.0001) were decreased in the patients as compared to controls. …”
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Electrochemical Glucose Sensor Based on Dual Redox Mediators by Changyun Quan, Yue Zhang, Yuanyuan Liu, Liping Wen, Haixia Yang, Xueqin Huang, Minghui Yang, Binjie Xu

“…The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. …”
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Programmable Multiplexed Nucleic Acid Detection by Harnessing Specificity Defect of CRISPR‐Cas12a by Xin Guan, Rui Yang, Jiongyu Zhang, Jeong Moon, Chengyu Hou, Chong Guo, Lori Avery, Danielle Scarola, Daniel S. Roberts, Rocco LaSala, Changchun Liu

“…By harnessing the specificity defect of the CRISPR‐Cas12a enzyme, a dual‐mode detection strategy is designed, which includes approximate matching and precise querying of target sequences and develop a programmable multiplexed nucleic acid assay. …”
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Simvastatin Does Not Affect Vitamin D Status, but Low Vitamin D Levels Are Associated with Dyslipidemia: Results from a Randomised, Controlled Trial by Lars Rejnmark, Peter Vestergaard, Lene Heickendorff, Leif Mosekilde

“…Statin drugs act as inhibitors of the 3-hydroxy-3methylglutaryl coenzyme A (HMG-CoA) reductase enzyme early in the mevalonate pathway, thereby reducing the endogenous cholesterol synthesis. …”
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