Development of a Luciferase Immunosorbent Assay for Detecting Crimean–Congo Hemorrhagic Fever Virus IgG Antibodies Based on Nucleoprotein by Qi Chen, Yuting Fang, Ning Zhang, Chengsong Wan

“…In addition, we compared the efficacy of our LISA to a commercial enzyme-linked immunosorbent assay (ELISA). Our results demonstrated that the optimal antigen for detecting anti-CCHFV IgG was located within the stalk cut-off domain of the nucleoprotein. …”
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Glial cell line-derived neurotrophic factor in patients with age-related cataract by A.A. Shpak, A.B. Guekht, T.A. Druzhkova, A.A. Troshina, N.V. Gulyaeva

“…The concentration of GDNF was measured using an enzyme immunoassay. Results. The concentration of GDNF in the AH was 88.9±46.9 (2.0–212.5) pg/ml, in the BS – 201.1±44.6 (103.2–287.2) pg/ml, in the LF – 343.6±133.7 (172.8–683.0) pg/ml. …”
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Alleviation of Brain Injury-Induced Cerebral Metabolic Depression by Amphetamine: A Cytochrome Oxidase Histochemistry Study by Richard L. Sutton, David A. Hovda, Michael J. Chen, Dennis M. Feeney

“…Brain tissue was processed for cytochrome oxidase histochemistry, and 12 bilateral cerebral areas were measured, using optical density as an index of the relative amounts of the enzyme. Compared with that of the control groups, cytochrome oxidase in the injured animals was significantly reduced throughout the cerebral cortex and in 5 of II subcortical structures. …”
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Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin by Mariam Abdullah, Fazliny Abd. Rahman, Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Noor Hayaty Abu Kasim, Sabri Musa

“…Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail. …”
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Plasma α-Glutathione S-Transferase Evaluation in Patients with Acute and Chronic Liver Injury by Jolanta Czuczejko, Celestyna Mila-Kierzenkowska, Karolina Szewczyk-Golec

“…High concentration of alpha-glutathione S-transferase (α-GST) in the liver and its short half-life make this enzyme clinically useful in assessing hepatocellular damage. …”
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In Vitro and In Silico Cytotoxic and Antibacterial Activities of a Diterpene from Cousinia alata Schrenk by Almira Zhanzhaxina, Yerlan Suleimen, Ahmed M. Metwaly, Ibrahim H. Eissa, Eslam B. Elkaeed, Raigul Suleimen, Margarita Ishmuratova, Kydyrmolla Akatan, Walter Luyten

“…The presence of the essential pharmacophoric features of histone deacetylase (HDAC) inhibitors in the structure of grindelic acid encouraged us to run a molecular docking study against the HDAC enzyme to understand its mechanism of action on a molecular level. …”
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Effects of Lysozyme, Proteinase K, and Cephalosporins on Biofilm Formation by Clinical Isolates of Pseudomonas aeruginosa by Mohamed Eladawy, Mohammed El-Mowafy, Mohamed Mohamed Adel El-Sokkary, Rasha Barwa

“…The effects of lysozyme (hydrolytic enzyme) and proteinase K (protease) on biofilm formation were evaluated at different concentrations. …”
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Serum levels of neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, and cystatin-C in renal artery stenosis: A pilot study by Raghunandan Prasad, Reeta Choudhary, Anuradha Singh, Surabhi Agarwal, P Kaushik, Kritika Singh, Rajanikant R Yadav, Amar Singh Yadav, Dharmendra Singh Bhadauria, Hira Lal

“…Serum was collected from participants to check for serum levels of NGAL, KIM-1, and Cys-c by quantitative enzyme immunoassay. The data of RAS patients were compared and analyzed against the data of healthy controls. …”
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Geometric Simulation Approach for Grading and Assessing the Thermostability of CALBs by B. Senthilkumar, D. Meshachpaul, R. Rajasekaran

“…Candida antarctica lipase B (CALB) is a known stable and highly active enzyme used widely in biodiesel synthesis. In this work, the stability of native (4K6G) and mutant (4K5Q) CALB was studied through various structural parameters using conformational sampling approach. …”
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Effect of Periodontal Therapy on Crevicular Fluid Interleukin-6 and Interleukin-8 Levels in Chronic Periodontitis by Paschalina Goutoudi, Evdoxia Diza, Malamatenia Arvanitidou

“…IL-6 and IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). Results. …”
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The role of circadian rhythm regulator PERs in oxidative stress, immunity, and cancer development by Baimei Zhao, Eugenie Nepovimova, Qinghua Wu

“…PER1 orchestrates the cooperation of the enzyme GPX1, modulating mitochondrial dynamics in sync with daily rhythms and oxidative stress, thus regulating the mechanisms managing energy substrates. …”
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Synthesis and Evaluation of the Acetylcholinesterase Inhibitory Activities of Some Flavonoids Derived from Naringenin by The-Huan Tran, Thi-Thu-Hien Vo, Thi-Quynh-Nhi Vo, Thi-Cam-Nhung Cao, Thai-Son Tran

“…The molecular docking revealed that strong interactions with amino acid residue Ser200 of the catalytic triad and those of the peripheral region of the enzyme were crucial for strong effects against AChE. …”
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A Cytokine That Is Involved in Immune Responses in Chronic Hepatitis B Virus Patients by Noor R. Abady, Zeana Shakir Al-Al Hindi

“…Materials and Methods: There were four groups in the study: asymptomatic HBsAg carriers (n = 42), chronic hepatitis B (CHB, n = 57), liver cirrhosis (LC, n = 59), and the normal control group (n = 80). an Enzyme-Linked was used to measure the amounts of IL-17, while reverse transcription polymerase chain reaction was used to find IL-17 mRNA in peripheral blood mononuclear cells (PBMC). …”
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Effects and Mechanisms of Silibinin on Influenza A/H1N1 Pathogenesis in a Mouse Model by Mohsen Keshavarz, Mohsen Ghorbani, Forough Shamsizadeh, Haideh Namdari, Vahid Salimi, Farhad Rezaei

“…For this, bronchoalveolar lavage (BAL) fluids were collected from the thoracic cavity 5 days after the intervention, and viral quantification was performed using TaqMan Real-time PCR. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate IFN‐γ and IL-10 levels in serum and BAL samples. …”
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Estimates of Total Dietary Folic Acid Intake in the Australian Population Following Mandatory Folic Acid Fortification of Bread by Jacinta Dugbaza, Judy Cunningham

“…A modification to the triple enzyme microbiological method was used to measure folic acid in the individual bread samples. …”
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Toward <i>Mycobacterium tuberculosis</i> Virulence Inhibition: Beyond Cell Wall by Maria S. Kotliarova, Mikhail S. Shumkov, Anna V. Goncharenko

“…Additionally, models for the search of virulence inhibitors are discussed, such as virtual screening in silico, in vitro enzyme inhibition assay, the use of recombinant Mtb strains with reporter constructs, phenotypic analysis using in vitro cell infection models and specific environments.…”
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An In Silico Study of the Interactions of Alkaloids from Cryptolepis sanguinolenta with Plasmodium falciparum Dihydrofolate Reductase and Dihydroorotate Dehydrogenase by Lois Kwane Kyei, Edward Ntim Gasu, Gilbert Boadu Ampomah, Jehoshaphat Oppong Mensah, Lawrence Sheringham Borquaye

“…Also, our results show that alkaloids of the pg class can inhibit PfDHODH as FMN decoys, as well as direct enzyme inhibitors, thereby halting crucial protein function.…”
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Association of anti-phospholipid antibodies and hepatitis B virus infection in Najaf Governorate by Ameer Mohammed Kadhim, Hawraa Ameer Mubark, Dhefaf Hameed Al-Mudhafar, Zainab Hayder Abdulmajeed

“…They were tested for Hepatitis B core (HBc) total and immunoglobulin M (IgM) Abs and then tested for APLA by enzyme-linked immunosorbent assay (ELISA) technique. …”
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Pan-Cancer Analysis of B4GALNT1 as a Potential Prognostic and Immunological Biomarker by Hang Yi, Yiwen Lin, Yutong Li, Yeqi Guo, Ligong Yuan, Yousheng Mao

“…Gangliosides act as important roles in tumor progression. B4GALNT1 is a key enzyme in ganglioside biosynthesis. B4GALNT1 expression is linked to tumorigenesis and the prognosis of tumor patients. …”
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Haplotype tagging efficiency and tagSNP sets portability in worldwide populations in NAT2 gene by Audrey Sabbagh, Pierre Darlu, André Langaney, Estella S. Poloni

“…Genetic polymorphism in the NAT2 gene is responsible for pronounced interindividual differences in the acetylation activity of the N-acetyltransferase 2 (NAT2) enzyme, which plays a crucial role in the metabolism of many clinically useful drugs and exogenous chemicals. …”
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