Application of probiotic microcapsules Bacillus cereus BR2 with different doses for the prevention of Aeromonas hydrophila NFC1 infection in catfish Clarias sp. by Nurul Faoziyatunnisa, Munti Yuhana, Dinamella Wahjuningrum, Sri Nuryati, Usamah Afiff

“… This study aims to analyze the administration of microencapsulated probiotic Bacillus cereus BR2 through feed atdifferent doses on the survival, immune response, and digestive enzyme activity of catfish Clarias sp. infected with Aeromonas hydrophila NFC1. …”
Get full text

Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon by ZHANG Yating, LOU Xueyi, WU Jiarui, LIU Hongyuan, YE Binyu, XIAO Jiamin, LI Jiameng, MIAO Yining, CHEN Xinyan, LIU Yilin, TAN Yuyu, WANG Zhichao, LIU Peng

“…Moreover, by conducting pot experiments, the influence of individual fungi, individual biochar, and combined biochar-fungi (AMF+10%B, AMF+20%B) on the physicochemical properties, and enzyme activities of saline soil and alkaline soil, and the photosynthetic parameters of muskmelon were studied. …”
Get full text

Lentivirus‐mediated gene therapy for Fabry disease: 5‐year End‐of‐Study results from the Canadian FACTs trial by Aneal Khan, Dwayne L. Barber, William M. McKillop, C. Anthony Rupar, Christiane Auray‐Blais, Graeme Fraser, Daniel H. Fowler, Alexandra Berger, Ronan Foley, Armand Keating, Michael L. West, Jeffrey A. Medin

“…Our goal was to correct the enzyme deficiency in Fabry patients by transferring the cDNA for α‐gal A into their CD34+ hematopoietic stem/progenitor cells (HSPCs). …”
Get full text

Plasma Levels of Tumor Necrosis Factor-Alpha and Interleukin-6 in Obsessive Compulsive Disorder by N. Konuk, I. O. Tekın, U. Ozturk, L. Atik, N. Atasoy, S. Bektas, A. Erdogan

“…TNF-α and IL-6 concentrations in blood were determined by enzyme-linked immunosorbent assay (ELISA). Results. …”
Get full text

In Silico Screening, Synthesis and In Vitro Evaluation of Some Quinazolinone and Pyridine Derivatives as Dihydrofolate Reductase Inhibitors for Anticancer Activity by A. G. Nerkar, A. K. Saxena, S. A. Ghone, A. K. Thaker

“…Dihydrofolate reductase (DHFR) is the important target for anticancer drugs belonging to the class of antimetabolites as the enzyme plays important role in the de novo purine synthesis. …”
Get full text

Hypobaric Hypoxia Imbalances Mitochondrial Dynamics in Rat Brain Hippocampus by Khushbu Jain, Dipti Prasad, Shashi Bala Singh, Ekta Kohli

“…Mitochondrial morphology, distribution within neurons, enzyme activity of respiratory complexes, Δψm, ADP: ATP, and expression of fission/fusion key proteins were determined. …”
Get full text

The Difference in Interleukin-19 Serum on Degrees of Acne Vulgaris Severity by Moerbono Mochtar, Alamanda Murasmita, M. Eko Irawanto, Indah Julianto, Harijono Kariosentono, Fajar Waskito

“…Sample subjects were patients with acne vulgaris who met the inclusion criteria. Enzyme-linked immunosorbent assay (ELISA) study was applied to measure IL-19 serum. …”
Get full text

Protective Effect of Baccharis trimera Extract on Acute Hepatic Injury in a Model of Inflammation Induced by Acetaminophen by Bruno da Cruz Pádua, Joamyr Victor Rossoni Júnior, Cíntia Lopes de Brito Magalhães, Míriam Martins Chaves, Marcelo Eustáquio Silva, Maria Lucia Pedrosa, Gustavo Henrique Bianco de Souza, Geraldo Célio Brandão, Ivanildes Vasconcelos Rodrigues, Wanderson Geraldo Lima, Daniela Caldeira Costa

“…Furthermore, pretreatment with the extract decreases the activity of the enzyme SOD and increases the activity of catalase and the concentration of total glutathione. …”
Get full text

Assembly of Genetically Engineered Ionizable Protein Nanocage-based Nanozymes for Intracellular Superoxide Scavenging by Qiqi Liu, Zhanxia Gao, Xiangyun Zhang, Qiannan Duan, Yue Zhang, Adam C. Midgley, Li Jiao, Ruming Liu, Mingsheng Zhu, Deling Kong, Jie Zhuang, Xinglu Huang

“…Abstract Nanozymes play a pivotal role in mitigating excessive oxidative stress, however, determining their specific enzyme-mimicking activities for intracellular free radical scavenging is challenging due to endo-lysosomal entrapment. …”
Get full text

Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies by Meilan Liu, Tingting Lin, Liyuan Yao, Hongfeng Chen, Yu Lu, Zhengguo Tao, Haiquan Zhao, Sheng-Xiang Qiu, Liyun Zhao

“…The following virtual screening and enzyme inhibition experiments were performed to analyse the antibacterial mechanisms of the compounds from Galla chinensis. …”
Get full text

Regulatory Effect of Iguratimod on the Balance of Th Subsets and Inhibition of Inflammatory Cytokines in Patients with Rheumatoid Arthritis by Yunzhi Xu, Qi Zhu, Jinglve Song, Hongli Liu, Yutong Miao, Fan Yang, Feiyan Wang, Wenjing Cheng, Yebin Xi, Xiaoyin Niu, Dongyi He, Guangjie Chen

“…Frequencies of Th1, Th17, Tfh, and Treg were quantified using flow cytometry, and serum cytokines were detected by enzyme-linked immunosorbent assay. mRNA expression of cytokines and transcriptional factor were quantified by RT-PCR. …”
Get full text

Hepatic Cholesterol-25-Hydroxylase Overexpression Improves Systemic Insulin Sensitivity in Mice by Britta Noebauer, Alexander Jais, Jelena Todoric, Klaus Gossens, Hedwig Sutterlüty-Fall, Elisa Einwallner

“…Oxysterols are naturally occurring molecules that play important role in various metabolic and inflammatory processes and their levels are elevated in patients suffering from obesity and diabetes. 25-Hydroxycholesterol (25-OHC) is produced in cells from cholesterol by the enzyme cholesterol 25-hydroxylase (Ch25h) and is involved in lipid metabolism, inflammatory processes, and cell proliferation. …”
Get full text

Autoantibody Response to Murine Double Minute 2 Protein in Immunodiagnosis of Hepatocellular Carcinoma by Mei Liu, Su-jun Zheng, Yu Chen, Ning Li, Peng-fei Ren, Li-ping Dai, Zhong-ping Duan, Jian-Ying Zhang

“…In this study, autoantibody responses to MDM2 protein in HCC patient’s serum were evaluated by enzyme-linked immunosorbent assay (ELISA) and part sera were evaluated by Western blotting and indirect immunofluorescence assay. …”
Get full text

Clinical and genetic analysis of a Chinese family with GM1 gangliosidosis caused by a novel mutation in GLB1 gene by Biao Zhang, Xiao-li Huang, Xin-xin Lu, Heng-bin Huang, Yan-an Wu

“…The pathogenic sites of candidate genes were targeted, and the detected exon mutations were verified by Sanger sequencing. Enzyme activity of β-galactosidase was detected in 293T cells transiently transfected with plasmids encoding the detected mutations.ResultsTwo siblings in this family presented with neurological degeneration, and were classified as the late-infantile type. …”
Get full text

Risk Factors Associated with False Positive HIV Test Results in a Low-Risk Urban Obstetric Population by Tamara T. Chao, Jeanne S. Sheffield, George D. Wendel, M. Qasim Ansari, Donald D. McIntire, Scott W. Roberts

“…To examine risk factors for false positive HIV enzyme immunoassay (EIA) testing at delivery. Study Design. …”
Get full text

Serious issues with cryo-EM structures of human prothrombinase by James A. Huntington, Alexandre Faille, Fatma Isik Ustok

“…Thrombin is generated from prothrombin through sequential cleavage at two sites by the enzyme complex prothrombinase, composed of a serine protease, factor (f) Xa and a cofactor, fVa, on phospholipid membranes. …”
Get full text

Experimentally Induced Pulpal Lesion and Substance P Expression: Effect of Ketoprofen—A Preliminary Study by Gian Marco Abbate, Paola Sacerdote, Giada Amodeo, Alessandro Mangano, Luca Levrini

“…SP levels were determined by using a commercially available enzyme immunoassay (ELISA) kit. The subjects were randomly divided into two groups: group 1 received a dose of ketoprofen 30 minutes prior to the experimental procedure. …”
Get full text

The Diagnostic Role of Serum Inflammatory and Soluble Proteins on Dementia Subtypes: Correlation with Cognitive and Functional Decline by Candan Öztürk, Aynur Özge, Osman Özgür Yalın, İ. Arda Yılmaz, Nuran Delialioglu, Çilem Yıldız, Bahar Tesdelen, Cigdem Kudiaki

“…We measured levels of IL-1β, IL-2, IL-6, IL-18, TNF-α, β-Amlyloid 1–40 and α1-antichymotrypsin levels in plasma in groups of total 82 subjects with AD, MCI, VD and controls using enzyme-linked immunosorbent assay (ELISA) method. …”
Get full text

Oxidative Stress and Its Association with COVID-19: A Narrative Review by Hardi Rafat Baqi, Halgord Ali M. Farag, Abdel Hamid H. El Bilbeisi, Rafee Habib Askandar, Amany M. El Afifi

“…Especially the virus’s route to cell entry via angiotensin-converting enzyme 2 (ACE2) receptor is well known that leads to pathogenesis in angiotensin II (AT II) which are critical in NADH/NADPH oxidase inducing ROS generation. …”
Get full text

Adding glucose combined with microalgae to water improves the benefits of the fungal community on the whiteleg shrimp (Litopenaeus vannamei) culture by Bilal Raza, Jiangdong Ke, Lin Chen, Yikai Shi, Jinyong Zhu, Zeyu Shao, Zhongming Zheng, Kaihong Lu, Wen Yang

“…Moreover, Aspergillus was indentified as both the biomarkers and modular nodes in co-occurrence network, domonstrating its strong association with non-specific immune enzyme activities of shrimp. Network topology analysis showed that the N. oculata and glucose combined promoted the complexity and amplified the proportion of negative edges in the fungal network, thereby enhacing the stability of the fungal community. …”
Get full text