Role and Effective Therapeutic Target of Gut Microbiota in Heart Failure by Qiujin Jia, Hao Li, Huan Zhou, Xiaonan Zhang, Ao Zhang, Yingyu Xie, Yanyang Li, Shichao Lv, Junping Zhang

“…At the same time, regulating intestinal microflora through diet, probiotics, antibiotics, fecal transplantation and microbial enzyme inhibitors has grown up to be a potential treatment for many metabolic disorders.…”
Get full text

Humoral Autoimmune Responses to Insulin-Like Growth Factor II mRNA-Binding Proteins IMP1 and p62/IMP2 in Ovarian Cancer by Xinxin Liu, Hua Ye, Liuxia Li, Wenjie Li, Yi Zhang, Jian-Ying Zhang

“…In this study, autoantibody responses to two members (IMP1 and p62/IMP2) of IMPs were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting, and indirect immunofluorescence assay in sera from patients with ovarian cancer and normal human individuals. …”
Get full text

Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice by Xiao Meng Zhang, Hao Deng, Jin Dong Tong, Yi Zhen Wang, Xu Chao Ning, Xiu Hong Yang, Fang Qiang Zhou, Hui Min Jin

“…Both Pyr-ORS and 1% pyruvate showed comparable therapeutic effectiveness with controls of body weight and blood sugar, increases of blood insulin levels, and improvement of renal function and pathological changes. Aberrant key enzyme activities in glucometabolic pathways, AR, PK, and PDK/PDH, were also restored; indexes of oxidative stress and inflammation, NAD+/NADH ratio, and AGEs in the kidneys were mostly significantly preserved after pyruvate treatments. …”
Get full text

Matrix metalloproteinase-3 is a potent prognostic factor associated with cell proliferation and migration in prostate cancer by Ai Sato, Kiyoshi Takagi, Mio Yamaguchi-Tanaka, Jotaro Okushima, Yuto Yamazaki, Akihiro Ito, Takashi Suzuki

“…Matrix metalloproteinase-3 (MMP3) is an enzyme that degrades several ECM components and is implicated in human malignancies. …”
Get full text

Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics by Qiyan Li, Riran Zhu, Jun Li, Xiaobing Wang, Lihua Xu, Yanshen Li, Peng Li

“…A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. …”
Get full text

Antihepatitis B Virus Activity of a Protein-Enriched Fraction from Housefly (Musca domestica) in a Stable HBV-Producing Cell Line by Xuemei Lu, Xiaobao Jin, Jie Wang, Fujiang Chu, Jiayong Zhu

“…HBsAg and HBeAg in the culture medium were measured by enzyme-linked immunosorbent assay. HBV-DNA was quantified by fluorescent quantification PCR. …”
Get full text

Current Trends in Treatment for Acid-Dependent Diseases: Clinical Efficacy and Safety of Rabeprazole by O. D. Lopina, B. K. Nurgalieva, T. L. Lapina

“…A lower rabeprazole metabolic dependence on cytochrome P-450 enzyme system renders its antisecretory effect predictable and reduces the risk of interactions with other drugs metabolised through this system. …”
Get full text

IL-10 in Antilipopolysaccharide Immunity Against Systemic Klebsiella Infections by Tomislav Rukavina, Brigita Ticac, Vanja Vasiljev

“…We measured plasma levels of interleukin-10 at different time points by enzyme immunoassay and analyzed the relation between interleukin-10 and proinflammatory cytokines interleukin-6 and tumor necrosis factor-α in order to determine the association of these ratios with the outcome of infection. …”
Get full text

Efficient reduction of β-lactoglobulin allergenicity in milk using Clostridium tyrobutyricum Z816 by Qianru Zhao, Yuwei Wang, Zhengming Zhu, Quanyu Zhao, Liying Zhu, Ling Jiang

“…Finally, the concentration and digestibility of β-LG in actual samples was quantified using enzyme-linked immunosorbent assay (ELISA) and gastrointestinal digestion simulation experiments. …”
Get full text

Overview of Novel Antipsychotic Drugs: State of the Art, New Mechanisms, and Clinical Aspects of Promising Compounds by Letizia Biso, Marco Carli, Marco Scarselli, Biancamaria Longoni

“…In particular, we analyzed the main classes of investigational antipsychotics, such as monoamine, glutamate, acetylcholine, cannabinoid receptor modulators, enzyme inhibitors, ion channel modulators, and mixed receptor modulators. …”
Get full text

Circadian Rhythm and Stress Response in Droppings of Serinus canaria by Maura Turriani, Nicola Bernabò, Barbara Barboni, Gianluca Todisco, Luigi Montini, Paolo Berardinelli

“…Serinus canaria is a widespread domestic ornamental songbird, whose limited knowledge of biology make compelling studies aimed to monitor stress. Here, a commercial enzyme immunoassay was adopted to measure immunoreactive corticosterone (CORT) in single Serinus canaria dropping sample, to monitor the daily fecal excretion of CORT in birds bred singly or in-group and to detect the effect promoted by aviary or small transport cage restraint. …”
Get full text

New advances in biological preservation technology for aquatic products by Xiaobao Nie, Zhijie Zuo, Ruichang Zhang, Si Luo, Yongzhou Chi, Xiangyang Yuan, Chengwen Song, Yongjiang Wu

“…Moreover, the preservation mechanisms of bio-preservatives, including antioxidant activity, inhibition of spoilage bacteria and enzyme activity, and the formation of protective films are reviewed. …”
Get full text

NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy by Nik M. Mustapha, Joanna M. Tarr, Eva M. Kohner, Rakesh Chibber

“…Intracellular glucose concentration, ROS formation and Nε-(carboxymethyl) lysine (CML) content were measured using Amplex Red assay kit, dihydroethidium (DHE), and competitive immunoabsorbant enzyme-linked assay (ELISA), respectively. Results. …”
Get full text

Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway by Chenlong Wang, Chuangjiang Chen, MengYa Wang, Sajid Ur Rahman, Bin Wei, Hongyan Ding, Wanyue Huang, Xichun Wang

“…Additionally Rutin lowered the expression of p53, ALOX12, and ACSL4 while significantly improving cytokinesis, antioxidant enzyme activity, and SLC7A11, GPX4, Nrf2, FTH1, thereby inhibiting cellular ferroptosis. …”
Get full text

Tribbles 3 Regulates the Fibrosis Cytokine TGF-β1 through ERK1/2-MAPK Signaling Pathway in Diabetic Nephropathy by Luwei Zhang, Jinhang Zhang, Xinnong Liu, Shengli Liu, Jun Tian

“…To reveal the expression and possible role of tribbles homolog 3 (TRB3) in the incidence of type 2 diabetic nephropathy, we used immunohistochemistry, real-time quantitative PCR, western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to study the expression of TRB3, extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase (ERK1/2 MAPK), transforming growth factor β1 (TGF-β1), and collagen type IV in kidneys of db/db diabetic mice and in murine renal mesangial cells stimulated with high glucose. …”
Get full text

Species Differences in Paraoxonase Mediated Hydrolysis of Several Organophosphorus Insecticide Metabolites by Russell L. Carr, Mary Beth Dail, Howard W. Chambers, Janice E. Chambers

“…Paraoxonase (PON1) is a calcium dependent enzyme that is capable of hydrolyzing organophosphate anticholinesterases. …”
Get full text

Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model... by Ferizović Harisa, Spasojević Nataša, Janković Milica, Stefanović Bojana, Dronjak Slađana

“…One promising therapeutic strategy for treating different diseases is inhibiting the enzyme fatty acid amide hydrolase (FAAH), which increases the availability of endogenous cannabinoids. …”
Get full text

Toxicity of Anethole and the Essential Oils of Lemongrass and Sweet Marigold to the Parasitic Mite Varroa destructor and Their Selectivity for Honey Bee (Apis mellifera) Workers an... by Qodratollah Sabahi, Mollah Md. Hamiduzzaman, Juan S. Barajas-Pérez, Jose M. Tapia-Gonzalez, Ernesto Guzman-Novoa

“…The expression of AChE, a gene that regulates the production of acetyl cholinesterase, a detoxifying enzyme, was not altered in bees treated with the plant compounds at 48 h post-treatment. …”
Get full text

Underlying anti-hypertensive mechanism of the Mizuhopecten yessoensis derived peptide NCW in spontaneously hypertensive rats via widely targeted kidney metabolomics by Wenjun Xue, Wenzhu Zhao, Sijia Wu, Zhipeng Yu

“…The angiotensin-converting enzyme (ACE) inhibitory peptide NCW derived from Mizuhopecten yessoensis has been demonstrated to have significant in vivo anti-hypertensive effects, however, its anti-hypertensive mechanism is still not fully clarified. …”
Get full text

Solid-Phase Extraction of Pesticides by Using Bioinspired Peptide Receptors by Valentina Lanzone, Manuel Sergi, Marcello Mascini, Rossana Scarpone, Flavio Della Pelle, Michele Del Carlo, Giampiero Scortichini, Dario Compagnone

“…A virtual development of hexapeptide receptors bioinspired by the acetylcholinesterase enzyme active site is proposed. A semicombinatorial approach was applied to generate a virtual hexapeptides library with different affinity properties towards organophosphate and carbamate pesticides. …”
Get full text