An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) by Riyad Hossen, Myles Courtney, Alasdair Sim, Md Abdullah Al Kamran Khan, Heroen Verbruggen, Trevor Bringloe

“…Our method, involving an initial sample treatment, lysis buffer adjustment, and enzyme incubation alterations, outperformed the conventional CTAB and commercial kits in terms of DNA yield and purity. …”
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Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma by Mengzhu Sun, Laure Garnier, Romane Chevalier, Martin Roumain, Chen Wang, Julien Angelillo, Julien Montorfani, Robert Pick, Dale Brighouse, Nadine Fournier, David Tarussio, Stéphanie Tissot, Jean-Marc Lobaccaro, Tatiana V. Petrova, Camilla Jandus, Daniel E. Speiser, Manfred Kopf, Caroline Pot, Christoph Scheiermann, Krisztian Homicsko, Giulio G. Muccioli, Abhishek D. Garg, Stéphanie Hugues

“…We show in a lymphangiogenic murine melanoma that lymphatic endothelial cells (LECs) upregulate the enzyme Ch25h, which catalyzes the formation of 25-hydroxycholesterol (25-HC) from cholesterol and plays important roles in lipid metabolism, gene regulation, and immune activation. …”
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Shigellosis Caused by CTX-M Type ESBL Producing Shigella flexneri in Two Siblings of Rural Nepal: First Case Report from the Country by Narayan Prasad Parajuli, Govardhan Joshi, Bashu Dev Pardhe, Jyotsna Shakya, Anjeela Bhetwal, Shreena Shakya, Roshan Pandit, Sumesh Shreekhanda Shrestha, Puspa Raj Khanal

“…In MDR strains, the third-generation cephalosporins have been used for the treatment, but, unfortunately, emerging reports of enzyme mediated β-lactam resistance among Shigella isolates from various parts of the world have greatly compromised the therapy of pediatric dysentery. …”
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Oxidative stress induced protein aggregation via GGCT produced pyroglutamic acid in drug resistant glioblastoma by Deanna Tiek, Xiao Song, Xiaozhou Yu, Runxin Wu, Rebeca Iglesia, Alicia Catezone, Katy McCortney, Jordain Walshon, Craig Horbinski, Pouya Jamshidi, Rudolph Castellani, Robert Vassar, Jason Miska, Bo Hu, Shi-Yuan Cheng

“…Gamma (γ)-glutamylcyclotranserase (GGCT), an enzyme in the γ-glutamyl cycle for glutathione production, located on chromosome 7 which is commonly amplified in GBM is also increased upon resistance. …”
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Oxidative Stress and Heme Oxygenase-1 Regulated Human Mesenchymal Stem Cells Differentiation by Luca Vanella, Christopher Sanford, Dong Hyun Kim, Nader G. Abraham, Nabil Ebraheim

“…HO, the rate-limiting enzyme in heme catabolism, plays an important role during MSCs differentiation. …”
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Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli by Xiaofeng Liu, Jingru Zhou, Muhammad Zeeshan Ul Haq, Zhineng Fu, Guangtao Gu, Jing Yu, Ya Liu, Dongmei Yang, Huageng Yang, Yougen Wu

“…Under p-HBA stress, silencing the PcCDPK5 homologous gene in Nicotiana tabacum leads to decreased antioxidant enzyme activity and increased malondialdehyde (MDA) content, significantly accumulating reactive oxygen species (ROS) and affecting normal plant growth. …”
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Effects of Tai Chi Training on Antioxidant Capacity in Pre- and Postmenopausal Women by Attakorn Palasuwan, Daroonwan Suksom, Irène Margaritis, Suphan Soogarun, Anne-Sophie Rousseau

“…Besides the well-known effects of TC on balance, flexibility, and maximum leg extensor strength, TC (1) increased erythrocyte glutathione peroxidase activity—an aerobic training-responsive antioxidant enzyme—and plasma total antioxidant status and (2) decreased plasma total homocysteine, a cardiovascular risk marker. …”
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Prevalence of Antibody to Hepatitis C Virus in Saudi Blood Donors by Bandar Al-Knawy, Awad A El-Mekki, Jamal Hamdi, Raja Thiga, Anwar Sheikha

“…The prevalence of antibodies to hepatitis C virus (anti-HCV) was retrospectively determined using a second generation enzyme immunoassay in 3868 blood donors from the southern part of Saudi Arabia in an area with high prevalence of hepatitis B virus (HBV) infection. …”
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Citral Delays Postharvest Senescence of Kiwifruit by Enhancing Antioxidant Capacity under Cold Storage by Ling Wei, Chuying Chen, Chunpeng Wan, Ming Chen, Jinyin Chen

“…In contrast, citral treatment induces the activation of antioxidant enzyme system such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). …”
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12/15-Lipoxygenase Is an Interleukin-13 and Interferon-γ Counterregulated-Mediator of Allergic Airway Inflammation by Alexa R. Lindley, Margaret Crapster-Pregont, Yanjun Liu, Douglas A. Kuperman

“…Interleukin-13 increases expression of the arachidonic acid-metabolizing enzyme, 15-lipoxygenase-1, in a variety of cell types. 15-lipoxygenase-1 is dramatically elevated in the airways of subjects with asthma. …”
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Spontaneous Renal Artery Dissection in COVID-19 Pneumonia: Potential Danger of Cytokine Storm by Jitendra Parmar, Tapan Patel, Sandip Shah, Jay Kothari, Sameer Dani, Sagar Vyas

“…Cytokine storm or direct viral invasion of the vascular endothelial cells through surface angiotensin-converting enzyme 2 receptors may result in endothelial dysfunction which can potentially result in dissection. …”
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Quinoline and Quinazoline Alkaloids against COVID-19: An In Silico Multitarget Approach by Esraa M. O. A. Ismail, Shaza W. Shantier, Mona S. Mohammed, Hassan H. Musa, Wadah Osman, Ramzi A. Mothana

“…Three of them, namely, norquinadoline A, deoxytryptoquivaline, and deoxynortryptoquivaline, showed strong binding to the three targets, SARS-CoV-2 main protease, spike glycoprotein, and human angiotensin-converting enzyme 2. These alkaloids therefore have promise for being further investigated as possible multitarget drugs against COVID-19.…”
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Case Report: Surgery combined with extracorporeal membraneoxygenation for acute type A aortic dissection complicated with acute myocardial infarction by Jianming Xia, Yan Qiu, Shuo Chang, Ying Feng, Heng Zhang, Xiaoqi Wang

“…The 12-lead electrocardiogram revealed ST-segment depression, myocardial enzyme levels were significantly elevated. Emergency physicians diagnosed ATAAD with AMI, and emergency surgery was planned in collaboration with the cardiovascular surgery team. …”
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Involvement of Indoleamine 2,3-Dioxygenase in Impairing Tumor-Infiltrating CD8+ T-Cell Functions in Esophageal Squamous Cell Carcinoma by Ge Zhang, Wan-Li Liu, Lin Zhang, Jun-Ye Wang, Miao-Huan Kuang, Peng Liu, Yue-Hao Lin, Shu-Qin Dai, Jun Du

“…IDO expression and functional enzyme activity in ESCC cell lines could be induced by IFNγ. …”
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Regulation of enzymatic lipid peroxidation in osteoblasts protects against postmenopausal osteoporosis by Qiong-Yi Zhang, Hai-Biao Gong, Man-Ya Jiang, Fujun Jin, Guan Wang, Chang-Yu Yan, Xiang Luo, Wan-Yang Sun, Shu-Hua Ouyang, Yan-Ping Wu, Wen-Jun Duan, Lei Liang, Yun-Feng Cao, Xin-Xin Sun, Meijing Liu, Gen-Long Jiao, Hua-Jun Wang, Kurihara Hiroshi, Xiaogang Wang, Rong-Rong He, Yi-Fang Li

“…Estrogen regulates the transcription of glutathione peroxidase 4 (GPX4), an enzyme crucial for reducing phospholipid peroxides in osteoblasts. …”
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Ilex paraguariensis extract as an alternative to pain medications by Nowacki Luciana C., Stechman-Neto José, Schiefer Elberth M., Santos Andressa F., Stinghen Andréa E. M., Sassaki Guilherme L., De Souza Lauro M., Cristoff Killian E., De Souza Wesley M.

“…Hepatotoxicity, psycho-stimulant test and evaluation of enzyme markers for liver damage were also tested. …”
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Nutrient status alters developmental fates via a switch in mitochondrial homeodynamics by Jie Zhang, Suning Liu, Yang Li, Guanfeng Xu, Huimin Deng, Kirst King-Jones, Sheng Li

“…Pre-NRC starvation reduces prothoracicotropic neuropeptide hormone signaling, insulin signaling, and TORC1 activity in PG cells, which prevent mitochondrial fragmentation and import of Disembodied, a key steroidogenic enzyme. Ultimately, pre-NRC starvation causes severe mitophagy and proteasome dysfunction, blocking steroidogenesis and metamorphosis. …”
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An Escin-loaded Glutaraldehyde-Albumin nanoparticle system for enhancing anticancer activity on lung cancer A549 cells by Murugan Alwarkurichi Munusamy, Muruganantham Bharathi, Abdurahman Hajinur Hirad, Abdullah A. Alarfaj, Samer Hasan Hussein-Al-Ali, Shobana Sampath, Ali Kudumba

“…The NPs were validated through FT-IR and DLS studies, and the WST-1 assay results showed that the synthesized albumin-escin NPs effectively inhibited A549 cells, induced apoptosis, and increased ATPase activity, and LDH enzyme release. The expression of apoptotic proteins and oxidative markers increased, while antiapoptotic protein and antioxidant levels were reduced in the albumin-Escin NPs-treated A549 cells. …”
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Extending Shelf Life of Indonesian Soft Milk Cheese (Dangke) by Lactoperoxidase System and Lysozyme by Ahmad Ni’matullah Al-Baarri, Anang Mohamad Legowo, Septinika Kurnia Arum, Shigeru Hayakawa

“…Preserving dangke in this enzyme combination affected its hardness, but there was no remarkable change in the pH value. …”
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Identification of meibomian gland testosterone metabolites produced by tissue-intrinsic intracrine deactivation activity by Khanh Tien Nguyen Pham, Takahito Miyake, Tomo Suzuki, Shigeru Kinoshita, Yuki Hamada, Hikari Uehara, Mamiko Machida, Takeshi Nakajima, Emi Hasegawa, Masao Doi

“…Here, we performed radioactive tracer studies in combination with pharmacological enzyme inhibition, followed by targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, and found three androgen metabolites—androstanedione, androsterone, and epiandrosterone—in mouse and human meibomian glands. …”
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