Multi-protective effects of wheat embryo globulin on D-gal-induced aging mice by Aimei Liaoa, Xing Lyu, Jiarui Ma, Yinchen Hou, Ming Hui, Na Liu, Yi Zhao, Yixiang Cui, Jihong Huang

“…Aged mouse model were established by subcutaneous injection of D-galactose (D-gal), and the effects of WEG on learning, memory, and antioxidant capacity in aging mice were explored through behavioural tests and antioxidant enzyme activities determination. Compared with the Model group, WEG improved the percentage of the platform quadrant, increased the number of crossing platforms, and enhanced the identification indexs. …”
Get full text

Active learning-assisted directed evolution by Jason Yang, Ravi G. Lal, James C. Bowden, Raul Astudillo, Mikhail A. Hameedi, Sukhvinder Kaur, Matthew Hill, Yisong Yue, Frances H. Arnold

“…We apply ALDE to an engineering landscape that is challenging for DE: optimization of five epistatic residues in the active site of an enzyme. In three rounds of wet-lab experimentation, we improve the yield of a desired product of a non-native cyclopropanation reaction from 12% to 93%. …”
Get full text

Targeting MDM2 affects spastin protein levels and functions: implications for HSP treatment by Francesca Sardina, Federica Polverino, Sonia Valentini, Claudia Carsetti, Elisabetta Falvo, Giada Tisci, Silvia Soddu, Fabiola Moretti, Alessandro Paiardini, Cinzia Rinaldo

“…Abstract Spastin is a microtubule (MT) severing enzyme that regulates several cell functions associated with MT dynamics. …”
Get full text

Side-Alternating Vibration Training Improves Muscle Performance in a Patient with Late-Onset Pompe Disease by Aneal Khan, Barbara Ramage, Ion Robu, Laura Benard

“…Side-alternating vibration training (SAVT) was used for 15 weeks in a patient with Late-onset Pompe disease who had never used enzyme replacement or chaperone therapy. Prior to the use of SAVT, the patient had experienced declining muscle performance and her 6-minute walk distance decreased from 210 to 155 metres in 6 months. …”
Get full text

A Severe Case of Drug-Induced Liver Injury after Gemcitabine Administration: A Highly Probable Causality Grading as Assessed by the Updated RUCAM Diagnostic Scoring System by Ilenia Mascherona, Caterina Maggioli, Maira Biggiogero, Oreste Mora, Lucia Marelli

“…Common side effects include bone marrow suppression, fatigue, diarrhea, nausea, gastrointestinal upset, rash, alopecia, and stomatitis. Transient serum enzyme elevations could be observed during therapy, but clinically significant acute liver injury has been rarely associated with its use. …”
Get full text

A Parasite That Should not be Neglected in Patients with Ulcerative Colitis: Entamoeba histolytica by Murat Soylu, Abdurrahman Ekici, Selahattin Aydemir, Şehriban Yürektürk, Önder Akkaş

“…The samples collected were first analyzed macroscopically and then using native-Lugol, trichrome staining, and enzyme-linked immunosorbent assay (ELISA). Results: In the microscopic examination, Entamoeba spp. cysts were found in 22% of the ulcerative colitis patients and 2% of those in the control group. …”
Get full text

Involvement of Nitric Oxide in a Rat Model of Carrageenin-Induced Pleurisy by Masahiro Iwata, Shigeyuki Suzuki, Yuji Asai, Takayuki Inoue, Kenji Takagi

“…NOC-18 and L-arginine reduced the migration of inflammatory cells and edema, lowered oxidative stress, and normalized antioxidant enzyme activities. NO synthase inhibitors increased the exudate formation and inflammatory cell number, contributed to oxidative stress, induced an oxidant/antioxidant imbalance by maintaining high O−2, and enhanced the production of pro-inflammatory mediators. …”
Get full text

Grape stems as sources of tryptophan and selenium: Functional properties and antioxidant potential by Raquel Fernandes, Concepción Medrano-Padial, Rui Dias-Costa, Raúl Domínguez-Perles, Cláudia Botelho, Rúben Fernandes, Ana Novo Barros

“…Tryptophan, selenium, and phenolic compounds, which regulate cognitive functions, protein and enzyme synthesis, and antioxidant processes, were analysed. …”
Get full text

Nutrigenomic regulation of sensory plasticity by Hayeon Sung, Anoumid Vaziri, Daniel Wilinski, Riley KR Woerner, Lydia Freddolino, Monica Dus

“…Here, we show that the metabolic enzyme O-GlcNAc Transferase (OGT) moonlights on the chromatin of the D. melanogaster gustatory neurons to instruct changes in chromatin accessibility and transcription that underlie sensory adaptations to a high-sugar diet. …”
Get full text

Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody by Yuyao Zhang, Li Qian, Qian Zhang, Yu Li, Yu Liu, Dechen Jiang

“…The detection limit is almost two orders lower than the value using the classic enzyme-linked immunosorbent assay, which offers a new design to enhance ECL emissions and the resultant analytical performance.…”
Get full text

Machine learning based QSAR and Molecular Dynamics simulations in the structural design and mechanism of action of imidazole derivatives with anti-melanoma activity by Rosalvo Ferreira de Oliveira Neto, Sérgio Ruschi Bergamachi Silva, Cintia Emi Yanaguibashi Leal, Edilson Beserra de Alencar Filho

“…Additionally, the imidazopyrazine nucleus was compared to a known inhibitor of the Aurora Kinase enzyme, an important target in cancer therapy. Thus, strategic imidazopyrazines were subjected to comparative molecular dynamics calculations, providing inferences about their possible mechanisms of action. …”
Get full text

Development of tolerance to bedaquiline by overexpression of trypanosomal acetate: succinate CoA transferase in Mycobacterium smegmatis by Gloria Mavinga Bundutidi, Kota Mochizuki, Yuichi Matsuo, Mizuki Hayashishita, Takaya Sakura, Yuri Ando, Gregory Murray Cook, Acharjee Rajib, Frédéric Bringaud, Michael Boshart, Shinjiro Hamano, Masakazu Sekijima, Kenji Hirayama, Kiyoshi Kita, Daniel Ken Inaoka

“…We introduced into Mycobacterium smegmatis an alternative ATP production pathway by substrate-level phosphorylation, specifically through overexpression of trypanosomal acetate:succinate co-enzyme A (CoA) transferase (ASCT). Intriguingly, the overexpression of ASCT partially restored intracellular ATP levels and resulted in acquired tolerance to BDQ growth inhibition at low, but not high concentrations of BDQ. …”
Get full text

Current Concepts of Hyperinflammation in Chronic Granulomatous Disease by Nikolaus Rieber, Andreas Hector, Taco Kuijpers, Dirk Roos, Dominik Hartl

“…The underlying pathomechanisms driving hyperinflammatory reactions in CGD are poorly understood, but recent studies implicate reduced neutrophil apoptosis and efferocytosis, dysbalanced innate immune receptors, altered T-cell surface redox levels, induction of Th17 cells, the enzyme indolamine-2,3-dioxygenase (IDO), impaired Nrf2 activity, and inflammasome activation. …”
Get full text

A Case of Nonfunctioning Pituitary Carcinoma That Responded to Temozolomide Treatment by Haruko Morokuma, Takao Ando, Takuya Hayashida, Ichiro Horie, Naoko Inoshita, Fumi Murata, Ikuko Ueki, Kan Nakamura, Misa Imaizumi, Toshiro Usa, Atsushi Kawakami

“…There was an apparent antitumor response seen in MRI. MGMT, an enzyme antagonized by TMZ, was negative in the tumor. …”
Get full text

Determination of Cell Permeabilization and Beta-Galactosidase Extraction from Aspergillus oryzae CCT 0977 Grown in Cheese Whey by Caroline dos Santos Viana, Denise Renata Pedrinho, Luiz Rodrigo Ito Morioka, Hélio Hiroshi Suguimoto

“…The objective of this work was to define the parameters for the determination of cell permeabilization and extraction of the enzyme from Aspergillus oryzae CCT 0977 biomass, with high enzymatic activity. …”
Get full text

Crystallographic and computational characterization and in silico target fishing of six aromatic and aliphatic sulfonamide derivatives by Anh Van Nguyen, Anh Thi Ngoc Vu, Andrey N. Utenyshev, Valeriy Tkachev, Nadezhda Polyanskaya, Dmitriy Shchevnikov, Magrarita Vasil’eva, Hieu Tran-Trung, Xuan Ha Nguyen, Olga V. Kovalchukova

“…In silico analyses predicted low toxicity and potential enzyme inhibition, along with antiprotozoal properties, suggesting these compounds as candidates against protozoan pathogens. …”
Get full text

Identification of pathogenic bacteria by biosensors in water and wastewater by Sakineh Hargolzadeh, Soheila Kashanian, Maryam Nazari

“…Therefore, timely diagnosis of these pathogens is very important. the use of specialized personnel, machines, and tools in molecular methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) are expensive, and traditional methods such as plate culture are time-consuming. …”
Get full text

Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma by Karakurt Serdar, Kandir Sinan, Gökçek-Saraç Çiğdem

“…Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (p < 0.001), which leads the cells to trigger apoptosis. …”
Get full text

Biosynthetic Pathways and the Role of the Mas Receptor in the Effects of Angiotensin-(1–7) in Smooth Muscles by Marcio Augusto Fressatto de Godoy, Larissa Pernomian, Ana Maria de Oliveira, Satish Rattan

“…Ang-(1–7) is produced via degradation of Ang II by the human angiotensin converting enzyme, also known as ACE2. In the cardiovascular system, Ang-(1–7) has been shown to produce effects that are opposite to those of Ang II. …”
Get full text

Comparative effects of biocontrol agent and pathogen on Nicotiana tabacum: insights into fungal-plant interactions by Quanyu Yin, Zhichao Ren, Dongling Wu, Zhao Feng, Zhengkang Zhu, Amit Jaisi, Hui Wang, Mengquan Yang

“…T. harzianum significantly enhanced plant growth, biomass accumulation, root system development, and physiological attributes such as photosynthetic pigment levels and antioxidant enzyme activity. In contrast, P. nicotianae negatively impacted these parameters, inhibiting growth and physiological function. …”
Get full text