Evaluation of Southern African wild edible plants for potential herb-drug interactions through ex vivo p-glycoprotein and in vitro cytochrome P450 3A4 inhibitory effects by P.S. Kheoane, K.C. Mokhetho, S. Mokhele, K.C. Mbara, C.M. Leonard, T.H. Gadaga, C. Tarirai

“…However, these commonly used plant materials interact with orthodox or conventional drugs through both p-glycoprotein (P-gp) and cytochrome P450 enzymes inhibition. Hence, it is vital to explore the possibility of herb-drug interactions when concomitantly taking conventional drug dosage forms with some of the WEPs. …”
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The Combined Effect of Garlic (Allium sativum) and Turmeric (Curcuma longa) Extracts to Reduce the Adverse Effects of Aflatoxin B1 in Rats by Hadi Sarir, Saeedeh Zakerian, masood didarkhah

“…The use of garlic, turmeric extract, and their combination improves liver function indicators and reduces liver enzymes such as AST, ALT, ALP, and total bilirubin. …”
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Whole genome sequencing of a novel carrageenan-degrading bacterium Photobacterium rosenbergii and oligosaccharide preparation by Jing Chen, Jing Chen, Runmin Chen, Kit-Leong Cheong, Zhuo Wang, Rui Li, Xuejing Jia, Qiaoli Zhao, Xiaofei Liu, Bingbing Song, Saiyi Zhong, Saiyi Zhong

“…GDSX-4 exhibited remarkable carrageenan-degrading activity, with a specific enzyme activity of 46.94 U/mg. Optimal hydrolysis conditions were determined to be 40°C and pH 7.0, with the enzyme retaining 80% of its activity below 30°C and across a pH range of 4.0–10.0. …”
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Insights into microbiota community dynamics and flavor development mechanism during golden pomfret (Trachinotus ovatus) fermentation based on single-molecule real-time sequencing a... by Yueqi Wang, Qian Chen, Huan Xiang, Dongxiao Sun-Waterhouse, Shengjun Chen, Yongqiang Zhao, Laihao Li, Yanyan Wu

“…Volatile profiles resulted from the interactions among the microorganisms and derived enzymes, with the main metabolic pathways being amino acid biosynthesis/metabolism, carbon metabolism, and glycolysis/gluconeogenesis. …”
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Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations by Lucas Tricoli, Sunetra Sase, Julia L. Hacker, Vi Pham, Maxwell Chappell, Laura Breda, Stephanie N. Hurwitz, Naoto Tanaka, Carlo Castruccio Castracani, Amaliris Guerra, Zhongqi Hou, Lars Schlotawa, Karthikeyan Radhakrishnan, Matthew Hogenauer, Aoife Roche, John Everett, Frederic Bushman, Peter Kurre, Rebecca Ahrens-Nicklas, Laura A. Adang, Adeline L. Vanderver, Stefano Rivella

“…Insufficient enzyme levels can lead to the progression of motor symptoms, undermining treatment goals. …”
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Interactions of the emerging fungus Candida auris with Acanthamoeba castellanii reveal phenotypic changes with direct implications on the response to stress and virulence by Michele Ramos Valente, Lucas Martins Alcântara, Deborah Santos Cintra, Susana Ruiz Mendoza, Elisa Gonçalves Medeiros, Kamilla Xavier Gomes, Leandro Honorato, Marcos de Abreu Almeida, Carmen Baur Vieira, Joshua Daniel Nosanchuk, Diana Bridon da Graça Sgarbi, Marcia Ribeiro Pinto, Leonardo Nimrichter, Allan Jefferson Guimarães

“…However, both isolates were more tolerant to itraconazole and caspofungin, particularly MMC2, which showed differential expression of ergosterol biosynthesis enzymes and increased cell wall polysaccharides. This study reveals that interactions with A. castellanii modulate C. auris physiology and virulence, contributing to its environmental adaptability and resistance to antifungals.IMPORTANCECandida auris has emerged as a critical public health concern due to its resistance to multiple antifungal drugs and ability to survive on surfaces under harsh conditions, mainly due to biofilm formation. …”
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T Cell-Derived Apoptotic Extracellular Vesicles Ameliorate Bone Loss via CD39 and CD73-Mediated ATP Hydrolysis by Yang X, Zhou Y, Zhou F, Bao L, Wang Z, Li Z, Ding F, Kuang H, Liu H, Tan S, Qiu X, Jing H, Liu S, Ma D

“…We observed increased levels of extracellular ATP and a decrease in CD39 and CD73, key enzymes in ATP-to-adenosine conversion in bone marrow of OVX mice. …”
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Optimizing selenium-enriched yeast supplementation in laying hens: Enhancing egg quality, selenium concentration in eggs, antioxidant defense, and liver health by Longfei Zhang, Jianmin Zhou, Uchechukwu Edna Obianwuna, Cheng Long, Kai Qiu, Haijun Zhang, Xiaolong Qi, Shugeng Wu

“…Increased activities of liver enzymes including alanine transaminase, alkaline phosphatase, and aspartate transaminase, alongside elevated levels of uric acid were notable in the SY60 group (P < 0.05). …”
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Sleeve gastrectomy reveals the plasticity of the human gastric epithelium by Amit Elad, Botros Moalem, Dana Sender, Aya Bardugo, Ki-Suk Kim, Yhara Arad, Haya Benhayon, Ayelet Gal Etzyoni, Nehemia Greenstein, Aviv Halfon, Sarah Knapp, Michelle Malis, Bailey Peck, Itia Samuel, Amram Kupietzky, Saleh Daher, Esther Forkosh, David Hakimian, Tiberiu Hershcovici, Nadav Ilani, Lior Katz, Moshe Rottenstreich, Elez Vainer, Yuval Ishay, Eitan Zlotnick, Abed Nasereddin, Idid Shiff, Ariel Benson, Ronit Grinbaum, Siddhartha Mishra, Shlomi Kotler, Linda C. Samuelson, Darleen A. Sandoval, Rachel Ben-Haroush Schyr, Danny Ben-Zvi

“…Using histology, and single-cell RNA sequencing of the gastric epithelium in 12 women, we observed that SG is associated with an increase in a sub-population of acid-secreting parietal cells that overexpress respiratory enzymes and an increase in histamine-secreting enterochromaffin-like cells (ECLs). …”
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Cancer cells avoid ferroptosis induced by immune cells via fatty acid binding proteins by Maria Angelica Freitas-Cortez, Fatemeh Masrorpour, Hong Jiang, Iqbal Mahmud, Yue Lu, Ailing Huang, Lisa K. Duong, Qi Wang, Tiffany A. Voss, Claudia S. Kettlun Leyton, Bo Wei, Wai-Kin Chan, Kevin Lin, Jie Zhang, Efrosini Tsouko, Shonik Ganjoo, Hampartsoum B. Barsoumian, Thomas S. Riad, Yun Hu, Carola Leuschner, Nahum Puebla-Osorio, Jing Wang, Jian Hu, Michael A. Davies, Vinay K. Puduvalli, Cyrielle Billon, Thomas P. Burris, Philip L. Lorenzi, Boyi Gan, James W. Welsh

“…This involves lipid peroxidation and enzymes like lysophosphatidylcholine acyltransferase 3 (Lpcat3), which makes cells more prone to ferroptosis. …”
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Characterisation of pregnancy-induced alterations in apolipoproteins and their associations with maternal metabolic risk factors and offspring birth outcomes: a preconception and l... by Li Chen, Karen Mei-Ling Tan, Melvin Khee-Shing Leow, Kok Hian Tan, Jerry Kok Yen Chan, Shiao-Yng Chan, Yap Seng Chong, Peter D. Gluckman, Johan G. Eriksson, Markus R. Wenk, Sartaj Ahmad Mir

“…Plasma apolipoproteins were strongly associated with biochemical measures including lipidomic profiles, lipoprotein features and fat-soluble vitamins, as well as metabolic risk factors including glycaemic traits, liver enzymes, inflammatory markers, albumin, and blood pressure. …”
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Ultrasensitive interferons quantification reveals different cytokine profile secretion in inflammatory myopathies and can serve as biomarkers of activity in dermatomyositis by Loïs Bolko, Loïs Bolko, Loïs Bolko, Céline Anquetil, Céline Anquetil, Alba Llibre, Solène Maillard, Damien Amelin, Karim Dorgham, Vincent Bondet, Océane Landon-Cardinal, Océane Landon-Cardinal, Océane Landon-Cardinal, Ségolène Toquet, Ségolène Toquet, Kuberaka Mariampillai, Samuel Malatre, Alexandrine Mahoudeau, Baptiste Hervier, Mathieu Rodero, Guy Gorochov, Darragh Duffy, Olivier Benveniste, Olivier Benveniste, Yves Allenbach, Yves Allenbach

“…ObjectiveThe objective of this study was to evaluate the presence of different types of interferon in idiopathic inflammatory myopathies (IIM) and their subgroups using ultrasensitive cytokine detection techniques (SIMOA) and to assess their potential as activity biomarkers.MethodsDisease activity was measured at the time of serum collection and assessed by manual muscle testing eight (MMT8 score 0-150), muscle enzymes to calculate the Physician Global Assessment (PGA) (0-10). …”
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Duckweed: a starch-hyperaccumulating plant under cultivation with a combination of nutrient limitation and elevated CO2 by Ling Guo, Ling Guo, Yang Fang, Songhu Wang, Yao Xiao, Yanqiang Ding, Yanling Jin, Xueping Tian, Anping Du, Zhihua Liao, Kaize He, Shuang Chen, Yonggui Zhao, Li Tan, Zhuolin Yi, Yuqing Che, Lanchai Chen, Jinmeng Li, Leyi Zhao, Peng Zhang, Zhengbiao Gu, Fangyuan Zhang, Yan Hong, Qing Zhang, Hai Zhao

“…This phenomenon is associated with the regulation of DNA methylation and transcription factors as well as the significantly upregulated transcription levels and the increased activities of key enzymes involved in starch biosynthesis. Moreover, while nitrogen redistribution was increased, sucrose biosynthesis and transportation and lignocellulose biosynthesis were reduced. …”
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Design of the Inhibitors for Pseudorabies Virus Replication by Reinforcement Learning from HSV‑1 DNA Polymerase Inhibitors by Lin Wei, Yang Hu, Licheng Bai, Chenxu Xiao, Zhang Liu, Yun You, Keke Wang, Yunyuan Huang, Junfei Zhu, Jun Weng, Wenling Zhou, Han Li, Honghe Zhao, Zhiyong Wu, Meng Mei, Zigong Wei

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Ammonia-Assimilating Bacteria Promote Wheat (<i>Triticum aestivum</i>) Growth and Nitrogen Utilization by Yuqian Gao, Qi Zhang, Yuannan Chen, Yanqing Yang, Chenxiao Zhou, Jiayang Yu, Yanan Li, Liyou Qiu

“…However, root GS, GOGAT enzyme activities, and ammonia assimilation-related gene expressions were lower than the controls. …”
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Purification and characterization of arginine deiminase from Klebsiella pneumoniae by Taif Hussien Alameedy, Mohammed Abdullah Jebor

“…Results: After 5.9 folds of purification and 38.7% enzyme recovery, the specific activity of the purified enzyme reached 164.2 U/mg. …”
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Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver by Hossein Tayefi-Nasrabadi, Reza Rahmani

“…Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. …”
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Production and Characterization of Highly Thermostable β-Glucosidase during the Biodegradation of Methyl Cellulose by Fusarium oxysporum by Folasade M. Olajuyigbe, Chidinma M. Nlekerem, Olusola A. Ogunyewo

“…Optimized production of β-glucosidase using the cellulosic substrate that supported highest yield of enzyme was examined over 192 h fermentation period and varied pH of 3.0–11.0. …”
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A Novel Process to Prepare Chitosan Macrospheres without Shrinkage and its Application to Immobilize β-Galactosidase by Su-Fang Sun, Yan Zhang

“…A new process for the preparation of chitosan macrospheres, which was simple and practicable, was suggested in this paper and various chitosans with different molecular weight were used as materials to immobilize β-galactosidase and the chitosan macrospheres with the lowest molecular weight (500 000) was selected as enzyme immobilization carrier based on the highest enzyme activity. …”
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Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus by Ritian Jin, Guanglei Wei, Rong Lin, Wenfeng Lin, Jude Juventus Aweya, Duo Liang, Wuyin Weng, Shen Yang

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