P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury by Ruidong Cheng, Genying Zhu, Chengtao Ni, Rui Wang, Peng Sun, Liang Tian, Li Zhang, Jie Zhang, Xiangming Ye, Benyan Luo

“…The expression levels of P2Y2R, GFAP, nestin, Tuj1, and CD34 were detected by immunofluorescence staining, and the expression levels of TNF-α, IL-1β, and IL-6 were detected by enzyme-linked immunosorbent assay. The locomotor score in the model group was significantly lower than the sham group. …”
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Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus by Liwen Zhang, Yu Wan, Liang Ma, Kaihong Xu, Baojin Cheng

“…BALB/c mice (6-8 weeks old, 20±1.1 g) were used as study models. Enzyme-linked immunosorbent assay (ELISA), quantitative real time PCR, western blot analysis, immunohistochemical staining, and flow cytometry analysis were performed to examine relevant indicators. …”
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Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples by Yong DOU, Jing DONG, Cheng CHEN, Peihong HU, Xinchi LIU, Lingwei KONG

“…The changes in cell permeability, antioxidant enzyme activities, cellular soluble protein and nucleic acid leakage, malonaldehyde content, and reactive oxygen species of B. cinerea were also investigated. …”
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Evaluation of various RNA-seq approaches for identification of gene outrons in the flatworm Opisthorchis felineus by N. I. Ershov, D. E. Maslov, N. P. Bondar

“…One is based on the enzymatic elimination of overrepresented cDNAs, the other utilizes exonucleolytic degradation of uncapped RNA by Terminator enzyme. By using the outron-targeting methods, we were not able to obtain the enrichment of RNA preparations by processed outrons, which is most likely indicative of a rapid turnover of these trans-splicing intermediate products. …”
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Programmable Multiplexed Nucleic Acid Detection by Harnessing Specificity Defect of CRISPR‐Cas12a by Xin Guan, Rui Yang, Jiongyu Zhang, Jeong Moon, Chengyu Hou, Chong Guo, Lori Avery, Danielle Scarola, Daniel S. Roberts, Rocco LaSala, Changchun Liu

“…By harnessing the specificity defect of the CRISPR‐Cas12a enzyme, a dual‐mode detection strategy is designed, which includes approximate matching and precise querying of target sequences and develop a programmable multiplexed nucleic acid assay. …”
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Role and Effective Therapeutic Target of Gut Microbiota in Heart Failure by Qiujin Jia, Hao Li, Huan Zhou, Xiaonan Zhang, Ao Zhang, Yingyu Xie, Yanyang Li, Shichao Lv, Junping Zhang

“…At the same time, regulating intestinal microflora through diet, probiotics, antibiotics, fecal transplantation and microbial enzyme inhibitors has grown up to be a potential treatment for many metabolic disorders.…”
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Comparison of VIDAS and Radioimmunoassay Methods for Measurement of Cortisol Concentration in Bovine Serum by Daniela Proverbio, Roberta Perego, Eva Spada, Giada Bagnagatti de Giorgi, Angelo Belloli, Davide Pravettoni

“…The VIDAS cortisol test is an enzyme-linked fluorescent assay designed for the MiniVidas system. …”
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Factors associated with early-onset androgenetic alopecia: A scoping review. by Li-Ping Liu, Mary Adumo Wariboko, Xiao Hu, Zi-Han Wang, Qian Wu, Yu-Mei Li

“…Early-onset AGA may also be associated with hormonal profiles, 5α-reductase enzyme activity, androgen receptor genes, and some susceptibility loci. …”
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Expression of Angiogenesis Regulatory Proteins and Epithelial-Mesenchymal Transition Factors in Platelets of the Breast Cancer Patients by Hui Han, Fang-Li Cao, Bao-Zhong Wang, Xue-Ru Mu, Guang-Yao Li, Xiu-Wen Wang

“…Vascular endothelial growth factor (VEGF), platelet-derived growth factor BB (PDGF-BB), thrombospondin-1 (TSP-1), platelet factor 4 (PF4), and transforming growth factor-β1 (TGF-β1) were measured by enzyme-linked immunosorbent assay. Angiogenesis-associated expression of VEGF (2.1 pg/106 platelets versus 0.9 pg/106 platelets, P < 0.001), PF4 (21.2 ng/106 platelets versus 10.2 ng/106 platelets, P < 0.001), PDGF-BB (42.9 pg/106 platelets versus 19.1 pg/106 platelets, P < 0.001), and TGF-β1 (15.3 ng/106 platelets versus 4.3 ng/106 platelets, P < 0.001) differed in the PP samples of cancer and control subjects. …”
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Angiogenic Response to Major Lung Resection for Non-Small Cell Lung Cancer with Video-Assisted Thoracic Surgical and Open Access by Calvin S. H. Ng, Song Wan, Randolph H. L. Wong, Anthony M. H. Ho, Anthony P. C. Yim

“…Blood samples were collected preoperatively and postoperatively on days (POD) 1 and 3 for enzyme linked immunosorbent assay determination of angiogenic factors. …”
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Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells by Si-Zhe Li, Xin-Xing Jin, Xiao-Lei Ge, Ya-Gang Zuo, Hong-Zhong Jin

“…TSLP levels in sera and blister fluids were measured by enzyme-linked immunosorbent assay. The TSLP expression in the BP lesional skin was detected by immunohistochemical staining. …”
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Ameliorative Effects of Polygonatum kingianum Polysaccharides on Lipid Metabolism Disorders and Brain Function Impairments in Obese Mice by XU Yuncong, YAN Qiaojuan, ZHU Chunhua, YANG Shaoqing, LI Houjiang, JIANG Zhengqiang

“…To study the effects of Polygonatum kingianum polysaccharides (PKP) on lipid metabolism disorders and brain function impairments in obese mice, the obese mice were treated with PKP, prepared by enzyme-assisted extraction, for 12 weeks. Changes in body mass and blood lipids, as well as pathological changes in liver and adipose tissue were observed. …”
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Manganese biofortification in grapevine by foliar spraying improves volatile profiles of Cabernet Sauvignon grapes and wine sensory traits by Ruihua Ren, Miaomiao Wang, Lijian Zhang, Fuxian Ren, Bowei Yang, Huangzhao Chen, Zhenwen Zhang, Qingqing Zeng

“…Manganese (Mn) is involved in plant metabolism as an enzyme cofactor. However, the role of Mn in the formation of volatile compounds in grapes has rarely been studied. …”
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Serum Vascular Endothelial Growth Factor Levels before and after Intravitreous Ranibizumab Injection for Retinopathy of Prematurity by Xuting Chen, Lin Zhou, Qi Zhang, Yu Xu, Peiquan Zhao, Hongping Xia

“…Serum VEGF level was measured by the enzyme-linked immunosorbent assay (ELISA). Results. …”
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Could serum Raftlin and GPER-1levels be new biomarkers for early detection of non-small cell lung cancer? by Aykut Eliçora, Berrak Güven, Hüseyin Engin, Gokcen Tugba Çevik, Hüseyin Fatih Sezer

“…GPER-1 and Raftlin levels were measured by Enzyme-Linked Immunosorbent Assay (ELISA) in blood samples taken from patients diagnosed with lung cancer and healthy volunteers. …”
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Zinc oxide seed priming enhances drought tolerance in wheat seedlings by improving antioxidant activity and osmoprotection by Rasha M. El-Shazoly, A. A. Othman, Muhammad Saqlain Zaheer, Ahmed F. Al-Hossainy, Dalia A. Abdel-Wahab

“…Zinc is an essential element for several enzymes involved in metabolism, cell elongation, preservation of the strength and integrity of cell membranes, seed development, and resistance to environmental stress. …”
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Matrix metalloproteinase-3 is a potent prognostic factor associated with cell proliferation and migration in prostate cancer by Ai Sato, Kiyoshi Takagi, Mio Yamaguchi-Tanaka, Jotaro Okushima, Yuto Yamazaki, Akihiro Ito, Takashi Suzuki

“…Matrix metalloproteinase-3 (MMP3) is an enzyme that degrades several ECM components and is implicated in human malignancies. …”
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Evaluating Methods for Aflatoxin B1 Monitoring in Selected Food Crops Within Decentralized Agricultural Systems by Haadia Tanveer, Hannah Glesener, Blake Su, Brooke Bolsinger, Rosa Krajmalnik-Brown, Lee E. Voth-Gaeddert

“…The objectives of this study were: (1) to evaluate common AFB1 detection methods, including enzyme-linked immunosorbent assays (ELISA) and lateral-flow assays (LFA), validated via high-performance liquid chromatography (HPLC), focusing on their suitability for possible applications in decentralized, low-resource settings; and (2) to conduct a barriers-to-use assessment for commonly available AFB1 detection methods and their applicability in low-resource settings. …”
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Scurvy as a Cause of Leg Pain and Limp in Pediatric and Adolescent Patients by Christopher J. DeFrancesco, MD, David Kell, BA, Sean Owens, BS, Matt Grady, MD, Naomi Brown, MD

“…Vitamin C deficiency (VCD) results in collagen dysfunction due to decreased lysyl and prolyl hydroxylase enzyme activity. The resulting disease, known as scurvy, can present with various findings, including gingival bleeding, bruising, other skin lesions, poor wound healing, and tooth and bone abnormalities. …”
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Transcriptional Regulation of Δ6-Desaturase by Peroxisome Proliferative-Activated Receptor δ Agonist in Human Pancreatic Cancer Cells: Role of MEK/ERK1/2 Pathway by Maryam Darabi, Shima Byagowi, Shabnam Fayezi, Masoud Darabi, Shahab Mirshahvaladi, Mehdi Sahmani

“…The Δ6-desaturase (Δ6D), also known as fatty acid desaturase 2, is a regulatory enzyme in de novo fatty acid synthesis, which has been linked to obesity and diabetes. …”
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