The Use of Perovskia abrotanoides Extract in Ameliorating Heat Stress-Induced Oxidative Damage and Improving Growth Efficiency in Carp Juveniles (Cyprinus carpio) by Hossein Adineh, Saeed Zahedi, Morteza Yousefi, Zeynab Sedaghat, Sevdan Yilmaz, Ebrahim Gholamalipour Alamdari, Mohammad Farhangi

“…In conclusion, the results showed, for the first time, that dietary supplementation with ~1% PAE can improve growth performance, stimulated the digestive enzymes, and enchanced antioxidant status as well as immune parameters and prevented high-temperature stress of common carp.…”
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Investigation of the Protective Effect of Probiotic Lactobacillus plantarum Ep-M17 on the Hepatopancreas of Penaeus vannamei by Xiaoman Hu, Wenlong Xu, Hao Li, Bowen Lu, Yang Du, Jiong Chen

“…To address this knowledge gap, our present study investigated the histological changes, enzyme activity, gene transcription, and metabolite levels in the hepatopancreas of shrimp after a 4-week diet supplemented with Ep-M17. …”
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Urinary Microvesicle-Bound Uromodulin: A Potential Molecular Biomarker in Diabetic Kidney Disease by Neng-jun Lou, Yi-hong Ni, Hong-ying Jia, Jing-ti Deng, Lu Jiang, Feng-jie Zheng, Ai-li Sun

“…Urinary microvesicle-bound uromodulin and total urinary uromodulin levels were determined by enzyme-linked immunosorbent assay (ELISA). Our results showed that the levels of urinary microvesicle-bound uromodulin in DN1 and DN2 groups were significantly higher than those in control group and DM group (P<0.01). …”
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Association of Autoimmune Thyroid Disease with Anti-GAD Antibody ELISA Test Positivity and Risk for Insulin Deficiency in Slowly Progressive Type 1 Diabetes by Masahito Katahira, Hidetada Ogata, Takahiro Ito, Tsutomu Miwata, Megumi Goto, Shizuka Nakamura, Hiromi Takashima

“…We examined the factors influencing GADA determination by radioimmunoassay (GADA-RIA) and by enzyme-linked immunosorbent assay (GADA-ELISA). Sixty patients with SPT1D and 154 patients with type 2 diabetes were examined by both GADA-RIA and GADA-ELISA and for the presence of autoimmune thyroid disease (AITD). …”
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Active Antialopecia Chemical Identification of Merremia peltata Leaves and Computational Study toward Androgen Receptor Using Molecular Docking and Molecular Dynamic Simulation by Syawal Abdurrahman, Ruslin Ruslin, Aliya N. Hasanah, Resmi Mustarichie, Mus Ifaya

“…One of the methods developed to treat alopecia is through inhibition of the enzyme 5-α-reductase, which converts testosterone into its more potent metabolite, dihydrotestosterone (DHT). …”
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Helicobacter pylori CagA promotes gastric cancer immune escape by upregulating SQLE by Sifan Liu, Nan Zhang, Xu Ji, Shuyue Yang, Zheng Zhao, Peng Li

“…This study revealed that H. pylori CagA upregulated squalene epoxidase (SQLE) expression, a key enzyme in the cholesterol biosynthesis pathway. Elevated SQLE activity increased cellular palmitoyl-CoA levels, enhancing PD-L1 palmitoylation while decreasing its ubiquitination. …”
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Synthesis, Biological Activity, and Molecular Modeling Studies of Pyrazole and Triazole Derivatives as Selective COX-2 Inhibitors by Mohyeddin Assali, Murad Abualhasan, Hadeel Sawaftah, Mohammed Hawash, Ahmed Mousa

“…In the diaryltriazole derivatives, compound 15a showed the best inhibitory activity in comparison to all synthesized compounds including the reference celecoxib with IC50 = 0.002 μM and SI = 162.5 as it could better fit the extra hydrophobic pocket which is present in the COX-2 enzyme. Moreover, the docking study supports the obtained SAR data and binding similarities and differences on both isozymes.…”
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Promoting Immortalized Adipose-Derived Stem Cell Transdifferentiation and Proliferation into Neuronal-Like Cells through Consecutive 525 nm and 825 nm Photobiomodulation by Madeleen Jansen van Rensburg, Anine Crous, Heidi Abrahamse

“…The protein detection of an early neuronal marker, neuron-specific enolase (NSE), and late, ciliary neurotrophic factor (CNTF), was determined with enzyme-linked immunosorbent assays (ELISAs). The genetic expression was also explored through real-time PCR. …”
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Macular Changes in a Mucopolysaccharidosis Type I Patient with Earlier Systemic Therapies by Augusto Magalhães, Ana Maria Cunha, Rodrigo Vilares-Morgado, Elisa Leão-Teles, Esmeralda Rodrigues, Manuel Falcão, Ângela Carneiro, Jorge Breda, Fernando Falcão-Reis

“…To describe retinal findings in a patient with mucopolysaccharidosis type I (MPS I) that underwent an early treatment with hematopoietic stem cell transplantation (HSCT) and enzyme replacement therapy (ERT). Case Report. We describe a case of a 12-year-old female with a biochemical and genetic diagnosis of MPS I. …”
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The Protective Effect of Naringenin on Airway Remodeling after Mycoplasma Pneumoniae Infection by Inhibiting Autophagy-Mediated Lung Inflammation and Fibrosis by Yan Lin, Dan Tan, Qianna Kan, Zhen Xiao, Zhiyan Jiang

“…Here, we studied 124 children with or without MPP and compared inflammatory cytokines and fibrinogen-related protein expression with enzyme-linked immunosorbent assay. We also employed a BALB/c mouse model of MPP and divided the mice into three groups: ctrl (normal control mice), MPP (MP-infected mice), and MPP + NRG (MP-infected mice treated with NRG). …”
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Study of Phenotypic and Molecular Approaches of Potyvirus plumpoxi Resistance Evaluation in New Czech Apricot Hybrids by Eliška Zezulová, Tomáš Kiss, Kateřina Baránková, Tomáš Nečas

“…This study investigates the resistance of 17 apricot hybrids to Potyvirus plumpoxi (PPV) with the use of phenotyping, serological assays [double-antibody-sandwich enzyme-linked immunosorbent assay (DAS-ELISA)], and molecular marker analyses. …”
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Seroprevalence of <i>Borrelia</i>, <i>Anaplasma</i>, <i>Bartonella</i>, <i>Toxoplasma</i>, <i>Mycoplasma</i>, <i>Yersinia</i>, and <i>Chlamydia</i> in Human Population from Eastern... by Angelina Wójcik-Fatla, Anna Sawczyn-Domańska, Anna Kloc, Joanna Krzowska-Firych, Jacek Sroka

“…Anti-<i>Borrelia burgdorferi</i> sensu lato (s.l.) antibodies were tested with ELISA and Western blot (WB) tests; the detection of anti-<i>Toxoplasma gondii</i> antibodies was performed using an enzyme linked fluorescence assay (ELFA). Antibodies to bartonellosis, anaplasmosis, and chlamydiosis were determined by indirect immunofluorescent test (IFA), whereas antibodies to yersiniosis and mycoplasmosis were confirmed in the ELISA test. …”
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Adenosine-Induced NLRP11 in B Lymphoblasts Suppresses Human CD4+ T Helper Cell Responses by Irem Ozel, Ilgin Akkaya, Ece Oylumlu, Goksu Uzel, Ceren Ciraci

“…NLRP11 was significantly upregulated after induction with adenosine at both the mRNA and protein levels, which led to the interaction of endogenous NLRP11 with the ASC adaptor protein; however, this interaction did not result in the activation of the caspase-1 enzyme. Furthermore, cocultures of NLRP11-expressing Burkitt’s lymphoma cells and naïve human peripheral CD4+ T lymphocytes had reduced IFN-γ and IL-17A production, whereas IL-13 and IL-10 cytokines did not change. …”
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Review of the potential of bioactive compounds in seaweed to reduce histamine formation in fish and fish products by Muhammad Maskur, Asep Awaludin Prihanto, Muhamad Firdaus, Rovina Kobun, Rahmi Nurdiani

“…However, simply relying on temperature control is insufficient, as certain bacteria can produce the enzyme histidine decarboxylase even at temperatures below 5°C. …”
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Antibacterial and Antioxidant Activities of Triterpenoids and Cyclic 1,7-Diarylheptanoids from the Stem Bark of Myrica salicifolia: A Combined Experimental and Computational Study by Abraham Dilnesa Gashaw, Kibrom Gebreheiwot Bedane, Taye B. Demissie, Japheth O. Ombito, Estifanos Ele Yaya, Mekonnen Abebayehu Desta

“…The lowest binding affinity for compound 7 was found to be −7.26 to −10.35 kcal/mol against PqsA protein of P. aeruginosa, pyruvate kinase (PK) enzyme of S. aureus, LuxS protein of S. pyogenes, and DNA gyrase B of E. coli, which showed better binding affinity compared to the standard drug ampicillin (−7.36 to −8.03 kcal/mol) and ciprofloxacin (−6.19 to −6.83 kcal/mol). …”
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Room temperature CRISPR diagnostics for low-resource settings by Eric A. Nalefski, Selma Sinan, Jason L. Cantera, Anastasia G. Kim, Remy M. Kooistra, Rafael E. Rivera, Jordyn P. Janshen, Sanchita Bhadra, Joshua D. Bishop, Andrew D. Ellington, Ilya J. Finklestein, Damian Madan

“…To mitigate these limitations, we identify the ortholog TsCas12a as a highly active nuclease at 25 °C and develop a single-protocol RPA-Cas12a detection reaction with this enzyme. A quantitative kinetic analysis reveals that fast nuclease activation is more critical than higher steady-state trans-cleavage activity for room temperature diagnostic applications. …”
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Terminalia catappa Extract Palliates Redox Imbalance and Inflammation in Diabetic Rats by Upregulating Nrf-2 Gene by Franklyn Nonso Iheagwam, Gaber El-Saber Batiha, Olubanke Olujoke Ogunlana, Shalom Nwodo Chinedu

“…Experimental rats were treated orally with 400 and 800 mg/kg bw TCA daily for four weeks. Antioxidant enzyme activities, plasma glucose concentration, protein concentration, oxidative stress, and inflammation biomarkers were assayed using standard methods. …”
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Plasma amino acid profiles of dogs with the hepatocutaneous syndrome and dogs with other chronic liver diseases by Rommaneeya Leela‐arporn, Karah Burns DeMarle, Cailin R. Heinze, Cynthia R. L. Webster

“…Dogs with chronic serum liver enzyme increases were recruited after hepatic biopsy. …”
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Anticataract Effect of the Aqueous Extract of the Flowers of Aspilia africana in Murine Model of Diabetic and Age-Related Cataracts by Adwoa Frema Amanfo, Samuel Kyei, Yaw Duah Boakye, Clement Osei Akoto, Justice Kwaku Addo, Kofi Oduro Yeboah, Newman Osafo

“…In vitro aldose reductase inhibitory property of the extract on rat lenses revealed that the AAE inhibited the enzyme activity with IC50 of 12.12 µg/ml. For the anticataract investigations, 30, 100, and 300 mg·kg−1AAE-treated rats recorded significantly low (p≤0.0001) cataract scores compared to the negative control rats, indicating a delay in cataractogenesis from the second week of treatment in the galactose-induced cataractogenesis. …”
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46,XY Disorder of Sex Development Caused by 17α-Hydroxylase/17,20-Lyase Deficiency due to Homozygous Mutation of CYP17A1 Gene: Consequences of Late Diagnosis by Giampaolo Papi, Rosa Maria Paragliola, Paola Concolino, Carlo Di Donato, Alfredo Pontecorvi, Salvatore Maria Corsello

“…Congenital adrenal hyperplasia (CAH) is an autosomal recessive disease due to specific enzyme deficiencies in the adrenal steroidogenesis pathway. …”
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