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1741
Diethyldithiocarbamate Delayed Wound-Induced Callus Formation in Potato Tubers by Inhibiting Superoxide Dismutase
Published 2024-12-01“…We determined the StSODs gene expression and activity of SOD, reactive oxygen species (ROS) content, phenylpropanoid metabolism-related enzyme activity, total phenols and lignin contents at the wound site during the healing process. …”
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1742
Clinical and genetic analysis of a Chinese family with GM1 gangliosidosis caused by a novel mutation in GLB1 gene
Published 2025-01-01“…The pathogenic sites of candidate genes were targeted, and the detected exon mutations were verified by Sanger sequencing. Enzyme activity of β-galactosidase was detected in 293T cells transiently transfected with plasmids encoding the detected mutations.ResultsTwo siblings in this family presented with neurological degeneration, and were classified as the late-infantile type. …”
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1743
Aqueous Levels of Pigment Epithelium-Derived Factor and Macular Choroidal Thickness in High Myopia
Published 2015-01-01“…Serum and aqueous levels of PEDF were measured by enzyme-linked immunosorbent assay in 36 high myopia patients (36 eyes) with no CNV (non-CNV group), 14 high myopia patients (14 eyes) with CNV (CNV group), and 42 nonmyopia patients (42 eyes) (control group). …”
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1744
Optimization of a fermented pumpkin-based beverage to improve Lactobacillus mali survival and α-glucosidase inhibitory activity: A response surface methodology approach
Published 2018-03-01“…Relationship between fermentation temperature, inoculum and ingredient concentration with response variables (fermentation time at the fermentation endpoint pH 4.5, survival rate of Lactobacillus mali K8 in pumpkin-based beverage treated with simulated gastrointestinal tract enzyme fluids, α-glucosidase inhibitory activity and sensory overall acceptability after 4 weeks of refrigerated storage) was investigated using response surface methodology. …”
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1745
Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination
Published 2025-02-01“…Changes in endogenous NO production, Fe content, starch mobilisation, and antioxidant enzyme activity were examined at the early (24 h) and late (72 h) germination stages. …”
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1746
MicroRNA-155 Participates in the Expression of LSD1 and Proinflammatory Cytokines in Rheumatoid Synovial Cells
Published 2020-01-01“…Moreover, RT-qPCR was used to explore the relative LSD1 miRNA expression in FLS when stimulated by miRNA-155 molecules, and Western blot and immunofluorescence assay were applied to probe into the expression level of LSD1. Finally, enzyme-linked immunosorbent assay was employed to analyze the secreting level of proinflammatory cytokines in FLS when stimulated by miRNA-155 molecules. …”
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1747
Changes in Circulating BDNF in relation to Sex, Diet, and Exercise: A 12-Week Randomized Controlled Study in Overweight and Obese Participants
Published 2019-01-01“…Using a previously published 12-week randomized study, serum BDNF was assessed at baseline and after 12 weeks using an enzyme-linked immunosorbent assay method. Fifty overweight or obese but healthy individuals (26 women, mean age of 36.4 ± 7.9 years; 24 men, mean age of 38.0 ± 5.9 years) were included and allocated into three groups: exercise-only (EXO; 12 weeks of aerobic exercise and isocaloric diet), diet-only (DIO; 8 weeks of very low energy diet (VLED 600 kcal/day) followed by a 4-week weight maintenance diet), or diet and exercise (DEX; 12 weeks of aerobic exercise in parallel with 8 weeks of VLED (800 kcal/day) followed by a 4-week weight maintenance diet). …”
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1748
TNF-α and Microalbuminuria in Patients with Type 2 Diabetes Mellitus
Published 2014-01-01“…Patients with microalbuminuria were older, with higher glycosylated hemoglobin levels (HbA1c) and they more frequently had diabetic retinopathy, neuropathy, and cardiovascular disease and were on treatment with angiotensin converting enzyme inhibitors (ACEi) and/or angiotensin receptor blockers (ARBs). …”
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1749
Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells
Published 2013-01-01“…The α1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y. …”
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1750
Antisense oligonucleotides for the arterial hypertension mechanisms study and therapy
Published 2018-04-01“…The review discusses the results of the use of titanium dioxide (TiO2) containing antisense nanocomposites for the angiotensin converting enzyme in rats with stress induced arterial hypertension (ISIAH). …”
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1751
Characterization of Autoantibodies against the E1𝜶 Subunit of Branched-Chain 2-Oxoacid Dehydrogenase in Patients with Primary Biliary Cirrhosis
Published 2012-01-01“…Sera with anti-BCOADC-E1𝛼 antibodies strongly inhibited the enzyme activity. These findings suggest that the E1𝛼 subunit as part of the native BCOADC complex is an immunogen, and that determinant spreading is involved in the pathogenesis of AMA production.…”
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1752
KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14)
Published 2025-02-01“…We find that KAI2-dependent signaling positively controls the expression of MpLONELY GUY (MpLOG), encoding a cytokinin biosynthesis enzyme. Disruption of the MpLOG function decreases endogenous cytokinin levels and causes defects similar to KAI2-dependent signaling mutants. …”
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1753
Vitamin E and N-Acetylcysteine as Antioxidant Adjuvant Therapy in Children with Acute Lymphoblastic Leukemia
Published 2009-01-01“…They were evaluated clinically for the occurrence of complications and by the laboratory parameters (blood levels of glutathione peroxidase (Glu.PX) antioxidant enzyme, malondialdehyde (MDA), tumor necrosis factor-𝛼 (TNF-𝛼), liver enzymes, and bone marrow picture). …”
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1754
RNA N4‐acetylcytidine modification and its role in health and diseases
Published 2025-01-01“…This review first outlines the key regulatory enzyme mediating ac4C modification, N‐acetyltransferase 10 (NAT10), including its critical roles in regulating RNA stability, transcriptional efficiency, and translational fidelity. …”
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1755
Biomarkers of Oxidative Stress in Huntington's Disease and Other Neurological Disorders: a Comparative Study
Published 2023-12-01“…<br /><strong>Results:</strong> there were significant differences in malonyldialdehyde in Huntington's disease compared to the control (p=0.02), but not with the rest of the groups. The enzyme superoxide dismutase in Huntington's disease was statistically lower compared to amyotrophic lateral sclerosis, although for catalase it was higher in relation to the rest of the patients. …”
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1756
Emodin promotes the recovery of rheumatoid arthritis by regulating the crosstalk between macrophage subsets and synovial fibroblast subsets
Published 2025-01-01“…In vitro experimental methods such as enzyme linked immunosorbent assay (ELISA), Western blotting, Transwell assays, CCK‐8 assays and cell coculture were used to assess cell proliferation, migration and secretion of inflammatory factors. …”
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1757
Analysis of Phenolic and Flavonoid Content, α-Amylase Inhibitory and Free Radical Scavenging Activities of Some Medicinal Plants
Published 2022-01-01“…The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the antioxidant potential. The α-amylase enzyme inhibition activity was performed to evaluate the antidiabetic activity of plant extracts. …”
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1758
The Diagnostic Role of Serum Inflammatory and Soluble Proteins on Dementia Subtypes: Correlation with Cognitive and Functional Decline
Published 2007-01-01“…We measured levels of IL-1β, IL-2, IL-6, IL-18, TNF-α, β-Amlyloid 1–40 and α1-antichymotrypsin levels in plasma in groups of total 82 subjects with AD, MCI, VD and controls using enzyme-linked immunosorbent assay (ELISA) method. …”
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1759
Antihepatitis B Virus Activity of a Protein-Enriched Fraction from Housefly (Musca domestica) in a Stable HBV-Producing Cell Line
Published 2014-01-01“…HBsAg and HBeAg in the culture medium were measured by enzyme-linked immunosorbent assay. HBV-DNA was quantified by fluorescent quantification PCR. …”
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1760
Optimization of Extraction of Compound Flavonoids from Chinese Herbal Medicines Based on Quantification Theory and Evaluation of Their Antioxidant Activity
Published 2022-01-01“…The highest CFs yield was obtained under the following extraction condition: liquid-solid ratio of 35 : 1 mL/g, extraction temperature of 75°C, extraction duration of 75 min, and extraction mode enzyme-assisted extraction. The forecasted yield was 37.62%. …”
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