Search for Anti-EA(D) Antibodies in Subjects with an “Isolated VCA IgG” Pattern by Massimo De Paschale, Debora Cagnin, Teresa Cerulli, Maria Teresa Manco, Carlo Agrappi, Paola Mirri, Arianna Gatti, Cristina Rescaldani, Pierangelo Clerici

“…However, one alternative is to use an enzyme-linked immunosorbent assay (ELISA) to detect anti-early antigen (EA) antibodies, which can be found in about 85% of subjects with acute Epstein-Barr virus (EBV) infections. …”
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Diarrheal Diseases Hospitalization in Yemen before and after Rotavirus Vaccination by Mohammed Amood AL-Kamarany, Lina Al-Areqi, Abulatif Mujally, Fawzya Alkarshy, Arwa Nasser, Aisha O. Jumaan

“…Rotavirus was detected by Enzyme Linkage Immunosorbent Assay (ELISA). The incidence of rotavirus hospitalization decreased from 43.79% in 2009 to 10.54% in 2014. …”
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Heterologous and High Production of Ergothioneine in <i>Bacillus licheniformis</i> by Using Genes from Anaerobic Bacteria by Zhe Liu, Fengxu Xiao, Yupeng Zhang, Jiawei Lu, Youran Li, Guiyang Shi

“…Given the value of EGT and the application of <i>Bacillus licheniformis</i> in enzyme preparation production, we cloned the key enzymes (EanA and EanB) from <i>Chlorbium limicola</i>. …”
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Effects of three kinds of growth regulators on flowering and branch physiological characteristics of Paeonia rockii by LIU Jiamiao, TANG Hong, ZHU Jiachen, TAO Xuanlin, XUE Nana

“…The changes of physiological indexes, including flowering at full-bloom stage, antioxidant enzyme activity, and osmotic adjustment substance content of branches, were investigated. …”
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Understanding Cardiovascular Events With JAK Inhibitors: Tofacitinib Reduces Synovial and Vascular Inflammation but not the Prothrombotic Effects of Inflammatory Cytokines on Endot... by Aliki Zavoriti, Pierre Miossec

“…A dose‐response of tofacitinib was tested on interferon γ, interleukin (IL) 17A, IL‐10, IL‐6, and IL‐1β, and cytokine production was measured by enzyme‐linked immunosorbent assay at 48 hours. Endothelial activation was induced with IL‐17A and tumor necrosis factor (TNF) or on contact with PBMCs. …”
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Evaluation of In-House ELISA for Antirabies Antibodies Detection in Domestic Canine by Yul Fitria, Niko Febrianto, Rahmi E. Putri, Ibenu Rahmadani, Didik T. Subekti

“…Serological methods for monitoring rabies-specific antibody titers can be carried out using enzyme-linked immunosorbent assay (ELISA) methods as recommended by the World Organization for Animal Health (WOAH). …”
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Effects of Dietary Aflatoxin B1 on Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Growth, Intestinal Health, and Muscle Quality by Hao Liu, Ruitao Xie, Weibin Huang, Yuanzhi Yang, Menglong Zhou, Baiquan Lu, Biao Li, Beiping Tan, Xiaohui Dong

“…In conclusion, diets with AFB1 levels exceeding 30 μg kg−1 inhibited hybrid grouper growth, while levels surpassing 445 μg kg−1 resulted in hindgut ROS accumulation, inflammation, elevated intestinal permeability, reduced digestive enzyme activity, and compromised muscle quality.…”
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Are horned cows in loose housing more stressed than hornless cows? a cross-sectional study in organic dairy farms by A. Ebinghaus, G. Thiessen, S. Ivemeyer, U. Knierim

“…Repeated faecal samples were collected from 674 cows (n = 2 625 samples), and FCMs were analysed by enzyme immunoassay. A total of 22 housing and management factors relating to guideline recommendations were recorded. …”
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Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice by Xiao Meng Zhang, Hao Deng, Jin Dong Tong, Yi Zhen Wang, Xu Chao Ning, Xiu Hong Yang, Fang Qiang Zhou, Hui Min Jin

“…Both Pyr-ORS and 1% pyruvate showed comparable therapeutic effectiveness with controls of body weight and blood sugar, increases of blood insulin levels, and improvement of renal function and pathological changes. Aberrant key enzyme activities in glucometabolic pathways, AR, PK, and PDK/PDH, were also restored; indexes of oxidative stress and inflammation, NAD+/NADH ratio, and AGEs in the kidneys were mostly significantly preserved after pyruvate treatments. …”
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Antibacterial Mechanisms of Constituents from Galla chinensis Revealed by Experimental and Virtual Screening-Based Studies by Meilan Liu, Tingting Lin, Liyuan Yao, Hongfeng Chen, Yu Lu, Zhengguo Tao, Haiquan Zhao, Sheng-Xiang Qiu, Liyun Zhao

“…The following virtual screening and enzyme inhibition experiments were performed to analyse the antibacterial mechanisms of the compounds from Galla chinensis. …”
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Comparison of Protein Acetyltransferase Action of CRTAase with the Prototypes of HAT by Prija Ponnan, Ajit Kumar, Prabhjot Singh, Prachi Gupta, Rini Joshi, Marco Gaspari, Luciano Saso, Ashok K. Prasad, Ramesh C. Rastogi, Virinder S. Parmar, Hanumantharao G. Raj

“…Our laboratory is credited for the discovery of enzymatic acetylation of protein, a phenomenon unknown till we identified an enzyme termed acetoxy drug: protein transacetylase (TAase), catalyzing the transfer of acetyl group from polyphenolic acetates to receptor proteins (RP). …”
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miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1 by Miaomiao Liu, Ying Zhang, Xiantong Cao, Tao Shi, Yang Yan

“…Then, we investigated the role of miR-197 in LPS-induced H9c2 cells by CCK-8 assay, flow cytometry, lactate dehydrogenase (LDH) measurement, enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blot. …”
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Optimization of Enzymatic Hydrolysis Process of Morchella esculenta and Analysis of Volatile Flavor Compounds in Enzymatic Hydrolysate by Xiaofeng ZENG, Mi YAN, Zhixing GE, Lunjiang GAO, Xumin YIN, Yuan DIAO, Sang SHANG, Shunde ZENG

“…Results indications were as follows: enzyme addition amount of 5000 U/g, pH6.7, enzymolysis temperature at 44 ℃ for 2 h, the hydrolysis degree was 34.11% under the above mentioned conditions and a total of 23 volatile flavor compounds were identified by GC-MS analysis, among which 17 werein blank group and 21 in enzymatic group. …”
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‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes by Xingli Zhang, Chong Teng, Kaidi Lyu, Shanhua Lyu, Yinglun Fan

“…The ligation efficiency was significantly higher because the restriction enzyme sites utilized were removed from the vector after being successfully constructed. …”
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The Potential Role of Matrix Metalloproteinases 8 and 9 and Myeloperoxidase in Predicting Outcomes of Bacterial Meningitis of Childhood by Okko Savonius, Irmeli Roine, Saeed Alassiri, Taina Tervahartiala, Otto Helve, Josefina Fernández, Heikki Peltola, Timo Sorsa, Tuula Pelkonen

“…MMP-9 levels in the CSF were assessed by zymography, while MMP-8, MPO, and TIMP-1 concentrations were determined with immunofluorometric and enzyme-linked immunosorbent assays. Results. MPO correlated positively with MMP-8 (rho 0.496, P<0.001) and MMP-9 (rho 0.153, P=0.02) but negatively with TIMP-1 (rho -0.361, P<0.001). …”
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Structural basis for RNA-guided DNA degradation by Cas5-HNH/Cascade complex by Yanan Liu, Lin Wang, Qian Zhang, Pengyu Fu, Lingling Zhang, Ying Yu, Heng Zhang, Hongtao Zhu

“…Notably, we also find that divalent ions such as zinc, cobalt, and nickel down-regulate enzyme activity by destabilizing the Cascade complex. …”
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Effect of Intensive Blood Pressure Control on Cardiovascular Remodeling in Hypertensive Patients with Nephrosclerosis by Otelio Randall, John Kwagyan, Tamrat Retta, Kenneth Jamerson, Velvie Pogue, Keith Norris, Muluemebet Ketete, Shichen Xu, Tom Greene, Xuelei Wang, Lawrence Agodoa

“…Subjects were randomly assigned to usual MAP goal (102–107 mmHg) or a lower MAP goal (≤92 mmHg) and randomized to beta-blocker, angiotensin converting enzyme inhibitor, or calcium channel blocker. After covariate adjustment, a higher PP was associated with increased risk of CV outcome (RR = 1.28, CI = 1.11–1.47, P<0.01) and new LVH (RR = 1.26, CI = 1.04–1.54, P=0.02). …”
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Evaluating the effect of virgin coconut oil pulling on viral load, bacterial load and inflammatory mediator levels in chronic periodontitis – A clinical study by Monika M, Rudhra K, Vennila K, Elanchezhiyan, Sri Harini P, S. Shakeron

“…Both pre- and post-treatment samples were subjected to real-time PCR (rtPCR) and enzyme-linked immunosorbent assay (ELISA) to quantify viral and bacterial loads, as well as levels of key inflammatory mediators. …”
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Strategic Feeding of Ammonium and Metal Ions for Enhanced GLA-Rich Lipid Accumulation in Cunninghamella bainieri 2A1 by Shuwahida Shuib, Wan Nazatul Naziah Wan Nawi, Ekhlass M. Taha, Othman Omar, Abdul Jalil Abdul Kader, Mohd Sahaid Kalil, Aidil Abdul Hamid

“…Reinstatement of the enzymes activities was achieved by feeding of ammonium tartrate, but no increment in the lipid content was observed. …”
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Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders by R. L. Luciano, A. L. S. P. Cardoso, G. F. Z. Stoppa, A. M. I. Kanashiro, A. G. M. de Castro, E. N. C. Tessari

“…Serum from commercial poultry breeders (𝑛=2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. …”
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