Usefulness of Immunological Detection of the Human Telomerase Reverse Transcriptase by Annalisa Volpi, Sara Bravaccini, Laura Medri, Serenella Cerasoli, Michele Gaudio, Dino Amadori

“…Whether hTERT protein detected by immunostaining with hTERT-specific Tel 3 36-10 antibody is actually the degraded form of the protein that retains hTERT antigenicity but not enzymatic function, or whether it represents the real, potentially functional catalytic subunit of the enzyme, immunohistochemistry would not seem to represent a useful tool to investigate the role of telomerase and the mechanisms involved in its regulation.…”
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Rheumatoid Arthritis Effects on Kidney and Liver and their Correlations with CDAI by Tamara Salman Mohammed, Raya Ezat Maroof, Ali Hussein Al-Hafidh

“…This study aims to assess the influence of RA duration on kidney and liver enzymes and study the correlation between these enzyme levels and RA disease activity (CDAI). …”
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A Pilot Seroprevalence Study Suggests Silent Zika virus Transmission in Bhopal Region of Central India by Shashwati Nema, Dipesh Kale, Romesh Jain, Ajay Halder, Manisha Shrivastava, Deepak Vaishnav, Ashvini Kumar Yadav, Divya Namdeo, Debasis Biswas

“…We tested blood samples of febrile patients for ZIKV RNA using real-time polymerase chain reaction (PCR), and for the healthy individuals, we determined anti-zika immunoglobulin G (IgG) antibodies using enzyme-linked immunosorbent assay. Results: ZIKV RNA was not detected in any of the 75 samples tested by real-time PCR assay. …”
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Discovery of Anti-inflammatory Peptides from Channa argus Using Virtual Screening, Molecular Docking, and Cell Model by XIANG Huan, LU Meiming, CHEN Shengjun, HUANG Hui, HU Xiao, ZHAO Yongqiang, WEI Ya

“…Virtual hydrolysis was used to select the best enzyme for obtaining bioactive peptides from C. argus proteins. …”
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Assessment of Some Biochemical Parameters in Iraqi Women with Recurrent Miscarriage by Ruqaya K. Abbas, Amani M. Jasim, Qater Al-nada A. Al-Ibady

“…Calcium and Zinc were evaluated in serum of miscarriage and healthy control group, serum Malondialdehyde MDA, an indicator of lipid peroxidation, serum SOD, antioxidant enzyme, and superoxide dismutase. The calcium concentration was low in aborted women compared with the healthy pregnant control group. …”
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A Case of Alport Syndrome with Posttransplant Antiglomerular Basement Membrane Disease despite Negative Antiglomerular Basement Membrane Antibodies by EIA Treated with Plasmapheres... by Sumiko I. Armstead, Thomas Hellmark, Jorgen Wieslander, Xin J. Zhou, Ramesh Saxena, Nilum Rajora

“…The serum anti-GBM IgG was negative by enzyme immunoassay (EIA). On biopsy, he had crescentic glomerulonephritis with linear GBM fixation of IgG. …”
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The genetic diversity of reed canarygrass (Phalaris arundinaceae L.) assessed by isozyme markers by R. S. Yudina, E. K. Khlestkina

“…In the current study, the genetic diversity of a reed canarygrass collection (42 populations collected in meadow biocenoses of several regions in Russia and some other countries) was investigated using isozyme markers IDH (isocitrate dehydrogenase), GDH (glutamate dehydrogenase), MDH (malate dehydrogenase), ME (malic enzyme), and SKDH (shikimate dehydrogenase). Genetic control of these enzymes was determined in reed canarygrass for the first time. …”
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Development of a Luciferase Immunosorbent Assay for Detecting Crimean–Congo Hemorrhagic Fever Virus IgG Antibodies Based on Nucleoprotein by Qi Chen, Yuting Fang, Ning Zhang, Chengsong Wan

“…In addition, we compared the efficacy of our LISA to a commercial enzyme-linked immunosorbent assay (ELISA). Our results demonstrated that the optimal antigen for detecting anti-CCHFV IgG was located within the stalk cut-off domain of the nucleoprotein. …”
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Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide by Jeffrey S. Rush, Svetlana Zamakhaeva, Nicholas R. Murner, Pan Deng, Andrew J. Morris, Cameron W. Kenner, Ian Black, Christian Heiss, Parastoo Azadi, Konstantin V. Korotkov, Göran Widmalm, Natalia Korotkova

“…The minor Glc modifications are β-Glc linked to position 4 of 3-rhamnose installed by SccP and SccQ glycosyltransferases, and α-Glc attached to position 4 of 2-rhamnose installed by SccN working in tandem with an unknown enzyme. Both the major and the minor β-Glc modifications control bacterial morphology, but only the GroP and major Glc modifications are critical for biofilm formation.…”
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The Association between Platelet Glycocalicin and High Microsatellite Instability in Colorectal Cancer by Zeng-yao Liu, Qing-chun Jia, Wen Wang, Yu-xi Liu, Rui-tao Wang, Jia-yu Li

“…The glycocalicin levels were measured with an enzyme-linked immunosorbent assay, and the cut-off point was determined with the receiver-operating characteristics curve. …”
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Anderson–Fabry Disease: An Overview of Current Diagnosis, Arrhythmic Risk Stratification, and Therapeutic Strategies by Chiara Tognola, Giacomo Ruzzenenti, Alessandro Maloberti, Marisa Varrenti, Patrizio Mazzone, Cristina Giannattasio, Fabrizio Guarracini

“…Current therapeutic interventions, including enzyme replacement therapy and antiarrhythmic medications, are reviewed in the context of their efficacy and limitations. …”
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Chronic Benign Tubular Albuminuria From Compound Heterozygous Variants in : A Case Report by Adam Pietrobon, Mark D. Elliott

“…Interventions: Neither angiotensin-converting enzyme (ACE) inhibition nor angiotensin Type II (AT-II) receptor blockade reduced his degree of albuminuria. …”
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Resistance of <i>Wolbachia</i> to Trimethoprim: Insights into Genes Encoding Dihydrofolate Reductase, Thymidylate Synthase and Serine Hydroxymethyltransferase in the Rickettsiales by Ann M. Fallon

“…Bacterial and eukaryotic dihydrofolate reductase (DHFR) enzymes are essential for DNA synthesis and are differentially sensitive to the competitive inhibitors trimethoprim and methotrexate. …”
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Mesenchymal Stem Cells in Renal Fibrosis: The Flame of Cytotherapy by Quan Zhuang, Ruoyu Ma, Yanshuang Yin, Tianhao Lan, Meng Yu, Yingzi Ming

“…Yet, the current method of treating renal fibrosis is fairly limited, including angiotensin-converting enzyme inhibition, angiotensin receptor blockade, optimal blood pressure control, and sodium bicarbonate for metabolic acidosis. …”
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Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin by Mariam Abdullah, Fazliny Abd. Rahman, Nareshwaran Gnanasegaran, Vijayendran Govindasamy, Noor Hayaty Abu Kasim, Sabri Musa

“…Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail. …”
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The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> by Xueying Yang, Wenjuan Zhao, Hui Li, Zhenxia Zhao, Jianbo Zhu, Jin Li

“…When exposed to low temperatures, we found that <i>PsLEA4</i> overexpression can regulate proline metabolism and antioxidant enzyme activity in tomatoes compared to wild tomatoes. …”
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Rosiglitazone and Pioglitazone Alter Aromatase Kinetic Properties in Human Granulosa Cells by Takako Araki, Miroslava Varadinova, Michael Goldman, Zev Rosenwaks, Leonid Poretsky, Donna Seto-Young

“…In the current paper, we explore the effects of rosiglitazone and pioglitazone on the aromatase enzyme kinetic properties in human granulosa cells. …”
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Antidiabetic Potential of Senna siamea: α-Glucosidase Inhibition, Postprandial Blood Glucose Reduction, Toxicity Evaluation, and Molecular Docking by Suthinee Sangkanu, Armad Heemman, Sathianpong Phoopha, Thanet Pitakbut, Wandee Udomuksorn, Sukanya Dej-adisai

“…The ethanolic extract of S. siamea heartwood exhibited activity against α-glucosidase enzyme with IC50 values of 54.4 μg/mL. Moreover, S. siamea extract (250–1000 mg/kg BW) was tested using normal rats with and without sucrose of 3 g/kg BW administration. …”
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Effects of different processing methods on the lipid composition of hazelnut oil: A lipidomics analysis by Jiayang Sun, Xiaonuo Feng, Chunmao Lyu, Shuang Zhou, Zixuan Liu

“…Here, we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil (CPO), ultrasonic-assisted hexane hazelnut oil (UHO) and enzyme-assisted aqueous hazelnut oil (EAO). A total of 10 subclasses of 98 lipids were identified, including 35 glycerolipids (GLs), 56 glycerophospholipids (GPs) and 7 sphingolipids (SPs). …”
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Borassus aethiopum (Mart.) ethanol fruit extract reverses alloxan-treatment alterations in experimental animals by Moses Dele, Adams, Ejike, Daniel Eze

“…While alloxan substantially (p < 0.05) increased the levels of some biological molecules and enzyme activity, it lowered those of others. The extract however significantly (p < 0.05) reversed all the alloxan-induced alterations, with the extract at 100 mg/kg b.w producing figures that compared (p > 0.05) well with those of the d.w treated non-diabetic animals and metformin-treated diabetic animals. …”
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