A Case–Control Study of the Association of Novel Androgens (11-KT and DHT), Classical Androgens (Testosterone and DHEAS), Hormones (LH, FSH, 17-OHP, Insulin, and SHBG), Insulin Res... by Nirmal Kumar, Neera Sharma, Smita Roy, Indu Chawla, Lokesh Kumar Sharma

“…The estimation of testosterone, DHEAS, 11-KT, and DHT was performed by the enzyme-linked immunoassay (ELISA) method. The assessment of biochemical and hormonal parameters was compared and correlated with 11-KT and DHT. …”
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HIV and the Gut Microbiota, Partners in Crime: Breaking the Vicious Cycle to Unearth New Therapeutic Targets by Kishanda Vyboh, Mohammad-Ali Jenabian, Vikram Mehraj, Jean-Pierre Routy

“…Microbial translocation and their downstream effects such as increased indolamine 2,3-dioxygenase (IDO) enzyme expression and activity create a self-sustaining feedback loop which enhances HIV disease progression and constitute a vicious cycle of inflammation and immune activation combining viral and bacterial factors. …”
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Egg quality evaluation of japanese quail (Coturnix coturnix japonica) supplemented with powdered leaves of jute mallow (Corchorus olitorius) and siam weed (Chromolaena odorata) by Gemma M Guigue, Alejandro Jr B Dianon, Decem Mary Joice D Tolero

“…Additionally, the supplementation stimulates the digestive system, enhancing digestive enzyme production and feed utilization efficiency by improving liver function, as supported by [7]. …”
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Isolation and In Silico Anti-COVID-19 Main Protease (Mpro) Activities of Flavonoids and a Sesquiterpene Lactone from Artemisia sublessingiana by Roza I. Jalmakhanbetova, Yerlan M. Suleimen, Masayoshi Oyama, Eslam B. Elkaeed, Ibrahim. H. Eissa, Raigul N. Suleimen, Ahmed M. Metwaly, Margarita Yu. Ishmuratova

“…The isolated compounds were in silico examined against the COVID-19 main protease (Mpro) enzyme. Compounds 1–6 exhibited promising binding modes showing free energies ranging from −6.39 to −6.81 (kcal/mol). …”
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PENGARUH PEMBERIAN ISOLAT KATEKIN GAMBIR (Uncaria gambir Roxb) TERHADAP KADAR HORMON TESTOSTERON DAN JUMLAH SPERMATOZOA TIKUS Rattus Norvegicus JANTAN HIPERGLIKEMIA by Ririn Marta Sari, Rauza Sukma Rita, Eliza Anas

“…Metode Penelitian ini merupakan penelitian experimental, Pemberian Isolat Katekin Gambir, selanjutnya dilakukan pengambilan darah pada vena retro orbita tikus yang digunakan untuk pemeriksaan kadar hormon testosteron dalam serum, kadar testosteron  di analisis dengan tekhnik Enzyme-Linked Immunosorbent Assay (ELISA) dan Vas Deferrens tikus untuk pengambilan spermatozoa menghitung jumlah spermatozoa dengan analisa sperma. …”
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Rat Hippocampal Neural Stem Cell Modulation Using PDGF, VEGF, PDGF/VEGF, and BDNF by Neus Gomila Pelegri, Catherine A. Gorrie, Jerran Santos

“…Cell growth and differentiation were assessed using immunohistochemistry and glutaminase enzyme assay. Cells were cultured for 14 days and treated with different growth factors at two different concentrations 20 ng/mL and 100 ng/mL. …”
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A Review on The Impact of Thermal Stress on Fish Biochemistry by Manoj Kumar Pati, Mohammad Aftabuddin, Prasun Roychowdhury

“…Other hormones (thyroid hormones, epinephrine, norepinephrine, insulin, glucagon) also play roles in the thermal stress response. Enzymes involved in metabolic pathways have optimal temperature and pH ranges altered by thermal stress. …”
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Vascular endothelial growth factor A: friend or foe in the pathogenesis of HIV and SARS-CoV-2 infections? by Mieke A. van der Mescht, Helen C. Steel, Ronald Anderson, Theresa M. Rossouw

“…We then focus on SARS-CoV-2 infection and show how the binding of the viral pathogen to the angiotensin-converting enzyme 2 receptor, as well as to NRP-1, leads to elevated levels of VEGF-A and consequences such as coagulation, vascular dysfunction, and inflammation. …”
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Extracorporeal Cardiac Shock Wave Therapy Ameliorates Clinical Symptoms and Improves Regional Myocardial Blood Flow in a Patient with Severe Coronary Artery Disease and Refractory... by Christian Prinz, Oliver Lindner, Thomas Bitter, Detlef Hering, Wolfgang Burchert, Dieter Horstkotte, Lothar Faber

“…There was no arrhythmia during or after treatment; enzyme levels were normal at all times. PET imaging showed a substantial improvement of myocardial stress perfusion. …”
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Optimization and Validation of Indirect ELISA Using Truncated TssB Protein for the Serodiagnosis of Glanders amongst Equines by Harisankar Singha, Praveen Malik, Sachin K. Goyal, Sandip K. Khurana, Chiranjay Mukhopadhyay, Vandana K. Eshwara, Raj K. Singh

“…In an attempt to develop recombinant protein based enzyme-linked immunosorbent assay (ELISA), N-terminal 200 amino acid sequences of B. mallei TssB protein—a type 6 secretory effector protein—were expressed in prokaryotic expression system. …”
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Predictive and Prognostic Value of sPRR in Patients with Primary Epithelial Ovarian Cancer by Katrin Kreienbring, Annika Franz, Rolf Richter, Duska Dragun, Harald Heidecke, Ralf Dechend, Dominik N. Muller, Jalid Sehouli, Elena I. Braicu

“…In this study 197 patients with primary EOC in our institution from 2000 to 2011 were included. sPRR was determined by enzyme-linked immunosorbent assay (ELISA) in preoperative taken blood sera. …”
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Ticlopidine in Its Prodrug Form Is a Selective Inhibitor of Human NTPDase1 by Joanna Lecka, Michel Fausther, Beat Künzli, Jean Sévigny

“…Ticlopidine (100 µM) completely inhibited the ATPase activity of NTPDase1 in situ as shown by enzyme histochemistry with human liver and pancreas sections. …”
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Combinatorial protective effect of Cinnamomum verum and Stingless Bee Honey against oxidative stress in isoproterenol-induced cardiotoxicity in Wistar rats by Arul Mozhi Padmanabhan, Victor Arokia Doss

“…Biochemical assays were conducted to measure antioxidant enzyme activities (Superoxide Dismutase, Catalase, Glutathione Peroxidase), non-enzymatic antioxidants (Vitamin C, Vitamin E, Reduced Glutathione), inflammatory markers, and oxidative stress levels. …”
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WY-14643, a Potent Peroxisome Proliferator Activator Receptor-α PPAR-α Agonist Ameliorates the Inflammatory Process Associated to Experimental Periodontitis by Enrico Briguglio, Rosanna Di Paola, Irene Paterniti, Emanuela Mazzon, Giacomo Oteri, Giancarlo Cordasco, Salvatore Cuzzocrea

“…Eighths days after placement of the ligature, we evaluated several markers of inflammation such us (1) myeloperoxidase activity, (2) a cytokines and adhesion molecules expression, (3) NF-κB expression, (4) iNOS expression, (5) the nitration of tyrosine residues, (6) activation of the nuclear enzyme poly(ADP-ribose) polymerase, (7) apoptosis, and (8) the degree of gingivomucosal tissues injury. …”
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Caffeine's Vascular Mechanisms of Action by Darío Echeverri, Félix R. Montes, Mariana Cabrera, Angélica Galán, Angélica Prieto

“…In endothelial cells, it increases intracellular calcium stimulating the production of nitric oxide through the expression of the endothelial nitric oxide synthase enzyme. Nitric oxide is diffused to the vascular smooth muscle cell to produce vasodilation. …”
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Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase by Gülşah P. Özgün, Emel B. Ordu, H. Esra Tütüncü, Emrah Yelboğa, Richard B. Sessions, Nevin Gül Karagüler

“…In NADH regeneration, Candida methylica formate dehydrogenase (cmFDH) is a highly significant enzyme in pharmaceutical industry. In this work, site saturation mutagenesis (SSM) which is a combination of both rational design and directed evolution approaches is applied to alter the coenzyme specificity of NAD+-dependent cmFDH from NAD+ to NADP+ and increase its thermostability. …”
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Hydroxychloroquine and Fabry Disease: Three Case Reports Examining an Unexpected Pathologic Link and a Review of the Literature by Rouhin Sen, Kathleen Borghoff, Kirk W. Foster, Stanley J. Radio, Alan Erickson, Michelene Hearth-Holmes

“…Lastly, we report the very interesting similarity in the intracellular action of hydroxychloroquine to the pathology of Fabry disease (and its associated lysosomal enzyme, α-galactosidase A). Case Presentation. We will examine the case presentations of three female Caucasian patients: a 22-year-old with lupus nephritis class V, a 72-year-old with long-standing systemic lupus erythematosus, and a 74-year-old with undifferentiated connective tissue disease. …”
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Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay by Raybel Muñoz, Jose A. Rodriguez, M. Elena Páez-Hernández, Irais Sánchez-Ortega, Eva M. Santos

“…The quantification of the hydroperoxides produced by reaction of lipoxygenase with linoleic acid allows the determination of the enzyme activity in cereal grains. Under optimal conditions, the linear range of the calibration curve ranges from 1.0 to 5.0 μM, with a limit of detection of 0.34 μM. …”
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Effect of Astaxanthin on Immune Function and Myocardial Injury in Mice with Chronic Sleep Deprivation by FENG Biyun, LI Lingyan, LI Ming, ZHANG Jiayuan, QI Huanyang, CHEN Pingping, XU Guanghui

“…Immune function and myocardial injury were evaluated by measuring organ coefficients as well as using the carbon clearance test and commercial enzyme-linked immunosorbent assay (ELISA) kits. The targets of astaxanthin and related biological processes and pathways were analyzed by network pharmacology. …”
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Challenges in Medicine: The Odyssey of a Patient with Isolated IgG4-Related Eosinophilic Angiocentric Fibrosis Presenting as a Locally Destructive Sinonasal Mass by Snigdha Nutalapati, Richard O’Neal, William O’Connor, Brett T. Comer, Gerhard C. Hildebrandt

“…Our case interestingly also had T-cell clonality and isolated isocitrate dehydrogenase 2 enzyme mutation on next-generation sequencing, suggesting a possible role of novel molecular-targeted therapies in this rare disease. …”
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