Preliminary results about the use of injected Lysozime (LIZOVET) to improve the response to synchronization protocol for insemination in buffalo heifers by Francisco Javier Vargas Ortiz, Johnny Ronnel Bracho Ylarraza

“… Lysozyme is a natural enzyme present in many body fluids in animals and humans, where it fulfills immunostimulant, bactericidal, virucidal, and anti-inflammatory functions. …”
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Synthesis, antibacterial evaluation and in silico studies of novel 2-(benzo[d]thiazol-2-yl)-N-arylacetamides and their derivatives as potential DHFR inhibitors by Nadia Hanafy Metwally, Galal Hamza Elgemeie, Aya Ragab Abdelrazek, Salwa Magdy Eldaly

“…Additionally, compounds 2c and 4m showed enzyme inhibition against dihydrofolate reductase in Escherichia coli with greater potency (IC50 for 2c = 3.796 µM, IC50 for 4m = 2.442 µM) than the standard antibiotic trimethoprim (IC50 = 8.706 µM). …”
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GABA promotes peroxisome proliferation in Triticum monococcum leaves by Yunus Şahin, Taras Nazarov, Ercan Selçuk Ünlü, Andrei Smertenko, Nusret Zencrici

“…CAT3 is an essential peroxisome biogenesis factor and a key enzyme of ROS homeostasis. Furthermore, exogenous application of GABA resulted in higher peroxisome abundance and transcriptional upregulation of BADH and a gene encoding another peroxisome biogenesis factor responsible for peroxisome fission, PEROXIN11C (PEX11C), in leaves. …”
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Exercise Training Prevents Decrease in Luminal Capillary Diameter of Skeletal Muscles in Rats with Type 2 Diabetes by Takeshi Morifuji, Shinichiro Murakami, Naoto Fujita, Hiroyo Kondo, Hidemi Fujino

“…The purpose of this study was to examine whether exercise training can prevent microangiopathy of skeletal muscles in rats with type 2 diabetes and if succinate dehydrogenase (SDH) activity, an indicator of mitochondrial oxidative enzyme activity, is involved in the prevention of microangiopathy. …”
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Serological Investigation of Akabane Virus Infection in Cattle and Sheep in Nigeria by Daniel Oladimeji Oluwayelu, Comfort Oluladun Aiki-Raji, Emmanuel Chibuzor Umeh, Samat Odunayo Mustapha, Adebowale Idris Adebiyi

“…Therefore, in investigating this disease, serum samples from 184 (abattoir and farm) head of cattle and 184 intensively reared sheep from two states in southwest Nigeria were screened for antibodies against AKAV using enzyme-linked immunosorbent assay. An overall seropositivity of 70.1% (129/184) was obtained with antibodies being detectable in 73.8% of abattoir (trade) cattle and 40.0% in farm cattle, while 4.3% (8/184) seropositivity was observed in sheep. …”
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Identification and characterization of a novel tetrapeptide from enzymatic hydrolysates of Baijiu byproduct by Qiang Wu, Changqing Zhong, Guirong Zeng, Xu Zhang, Liping Xiang, Chang Wan, Yougui Yu

“…In order to prepare angiotensin I-converting enzyme (ACE) inhibitory peptides, distilled spent grains of Chinese strong-flavor Baijiu were hydrolyzed by alcalase followed by papain under optimized conditions. …”
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Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody by Yuyao Zhang, Li Qian, Qian Zhang, Yu Li, Yu Liu, Dechen Jiang

“…The detection limit is almost two orders lower than the value using the classic enzyme-linked immunosorbent assay, which offers a new design to enhance ECL emissions and the resultant analytical performance.…”
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Effects of Endurance Training and Chlorogenic Acid on Oxidative and Nitrosative Stress Markers in Prediabetic Male Mice by Sahar Ghasemi Pour, Sayyed M. Marandi

“…Next, 24 hours after the last intervention, tissue samples were taken from the quadriceps femoris muscles and analyzed using the enzyme-linked immunosorbent assay (ELISA) method. …”
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Comparative evaluation of the efficacy of nicotine chewing gum and nicotine patches as nicotine replacement therapy using salivary cotinine levels as a biochemical validation measu... by T Sivasankari, Avudaiappan Sankaran, Senthil Murugappan, Veni Subramanyam, Balanehru Subramanian, RC Jagat Reddy, S Vandana

“…At baseline and at the end of the trial period, the saliva samples were analyzed for cotinine levels using an enzyme-linked immunosorbent assay (ELISA). The severity of smoking was assessed using the Fagerstrom Test for Nicotine Dependence (FTND). …”
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An indirect ELISA for the detection of antibodies against Dirofilaria spp. in cats by Lívia Perles, Mariaelisa Carbonara, Jairo Alfonso Mendoza-Roldan, Luigi Venco, Simona Gabrielli, Domenico Otranto

“…To improve detection of antibodies against Dirofilaria spp. in cats, an indirect enzyme-linked immunosorbent assay (ELISA) using somatic antigens of D. immitis was standardized. …”
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In Vitro α-Amylase and Protein Glycation Inhibitory Activity of the Aqueous Extract of Flueggea leucopyrus Willd by Vishmi Sachindra Thrikawala, Srianthie A. Deraniyagala, Chamira Dilanka Fernando, Dinusha Nishani Udukala

“…This study shows that Flueggea leucopyrus Willd not only inhibits α-amylase enzyme, which is known to break starch to glucose, but also inhibits the formation of AGEs, which occur due to chronic hyperglycemia that leads to the onset of diabetic complications.…”
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Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents by Mar Quiñones, Cintia Folgueira, Estrella Sánchez-Rebordelo, Omar Al-Massadi

“…Irisin levels were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Radioimmunoassay (RIA). …”
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Elevated Osteopontin Levels in Mild Cognitive Impairment and Alzheimer’s Disease by Yuan Sun, Xue Song Yin, Hong Guo, Rong Kun Han, Rui Dong He, Li Jun Chi

“…Concentrations of OPN in the CSF and plasma were determined by enzyme-linked immunosorbent assay. During a 3-year clinical followup, 13 MCI patients converted to AD (MCI converters), and 18 were clinically stable (MCI nonconverters). …”
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Occurrence of Bordetella Infection in Pigs in Northern India by Sandeep Kumar, Bhoj R. Singh, Monika Bhardwaj, Vidya Singh

“…In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs. …”
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Efficient Production of Fuel Ethanol via the Simultaneous Use of Distillery Stillage Biomass and Beet Molasses by Katarzyna Osmolak, Dawid Mikulski, Grzegorz Kłosowski

“…The maximum glucose concentration of about 12 g/L (hydrolysis efficiency about 35.5%) was achieved even with the lowest enzyme dose, i.e., 7.5 FPU per gram of biomass. The yeast also showed high fermentation activity in media prepared from stillage biomass and molasses, producing about 50 g/L of ethanol regardless of the fermentation strategy used. …”
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ATP-Binding Pocket-Targeted Suppression of Src and Syk by Luteolin Contributes to Its Anti-Inflammatory Action by Jeong-Oog Lee, Deok Jeong, Mi-Yeon Kim, Jae Youl Cho

“…In the present study, the anti-inflammatory mode of action in activated macrophages of luteolin from Artemisia asiatica was examined by employing immunoblotting analysis, a luciferase reporter gene assay, enzyme assays, and an overexpression strategy. Luteolin dose-dependently inhibited the secretion of nitric oxide (NO) and prostaglandin E2 (PGE2) and diminished the levels of mRNA transcripts of inducible NO synthase (iNOS), tumor necrosis factor- (TNF-) α, and cyclooxygenase-2 (COX-2) in lipopolysaccharide- (LPS-) and pam3CSK-treated macrophage-like RAW264.7 cells without displaying cytotoxicity. …”
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Plasminogen Activator Inhibitor-1 Level Correlates with Lipoprotein Subfractions in Obese Nondiabetic Subjects by Sándor Somodi, Ildikó Seres, Hajnalka Lőrincz, Mariann Harangi, Péter Fülöp, György Paragh

“…Plasma PAI-1, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and myeloperoxidase (MPO) concentrations were determined with enzyme-linked immunosorbent assay (ELISA), while serum paraoxonase-1 (PON1) activities were measured by spectrophotometry. …”
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Supplementation with Alpha-Tocopherol and Ascorbic Acid to Nonalcoholic Fatty Liver Disease’s Statin Therapy in Men by Nikola Hadzi-Petrushev, Katerina Dimovska, Nikola Jankulovski, Dine Mitrov, Mitko Mladenov

“…We hypothesized that the addition of antioxidant vitamins C and E to atorvastatin therapy is associated with improved serum enzyme antioxidant status. NAFLD-related serum parameters and the activity of antioxidant enzymes, before and after 3 months of treatment, were determined in patients receiving atorvastatin alone or atorvastatin plus antioxidants. …”
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Recent advances in genetics of aggressive behavior by J. D. Davydova, S. S. Litvinov, R. F. Enikeeva, S. B.  Malykh, E. K. Khusnutdinova

“…The most studied candidate genes for aggressive behavior are serotonergic (TPH1, TPH2, HTR2A, SLC6A4) and dopaminergic (DRD4, SLC6A3) system genes, as well as the serotonin or catecholamine metabolizing enzyme genes (COMT, MAOA). In addition, there is evidence that the hypothalamic-pituitary system genes (OXT, OXTR, AVPR1A, AVPR1B), the sex hormone receptors genes (ER1, AR), neurotrophin (BDNF) and neuronal apoptosis genes (CASP3, BAX) may also be involved in development of aggressive behavior. …”
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Coronary Artery Spasm: New Insights by Anthony Matta, Frederic Bouisset, Thibault Lhermusier, Fran Campelo-Parada, Meyer Elbaz, Didier Carrié, Jerome Roncalli

“…However, the prevalence of CAS tends to decrease in correlation with the increasing use of medicines such as calcium channel blockers, angiotensin converting enzyme inhibitor, and statins, the controlling management of atherosclerotic risk factors, and the decreased habitude to perform a functional reactivity test in highly active cardiac catheterization centers. …”
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