Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells by Juan E. Losa Garcia, María Rosa Martín de Cabo, Fernando Mateos Rodríguez, Jesús Pérez Losada, Antonio Jiménez López, José Luis Pérez Arellano

“…Interleukin-1g (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. At the highest concentration used (200 ng/mL) CsA decreased the basal and stimulated secretion of all the inflammatory cytokines studied in both undifferentiated and differentiated cells, with the only exception of PMA-stim ulated IL-1 secretion by undifferentiated cells. …”
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Development of animal feeding additives from mushroom waste media of shochu lees by Fumio Yagi, Yuji Minami, Masayoshi Yamada, Kyohei Kuroda, Masahito Yamauchi

“…Abstract Purpose Mushroom waste medium can be used as a source of enzymes, but few studies on animal feed improvement using it have been found. …”
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Targeting glycolytic reprogramming by tsRNA-0032 for treating pathological lymphangiogenesis by Fan Ye, Ziran Zhang, Lianjun Shi, Shuting Lu, Xiumiao Li, Wan Mu, Qin Jiang, Biao Yan

“…Overexpression of tsRNA-0032 reduces ATP production and decreases pyruvate and lactate levels by targeting PKM2, a key enzyme in glycolysis. This regulation of glycolysis alters cellular energy and metabolic balance in HLECs, contributing to anti-lymphangiogenic effects. …”
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Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis by Shima Karami, Behrouz Shiran, Rudabeh Ravash

“…On the other hand, it seems that in wheat, in order to deal with oxidative stress, the expression of homologous genes of C4 enzyme and genes involved in heme and siroheme synthesis pathway has increased under stress. …”
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Impact of developmental state, p53 status, and interferon signaling on glioblastoma cell response to radiation and temozolomide treatment. by Artem Berezovsky, Oluwademilade Nuga, Indrani Datta, Kimberly Bergman, Thais Sabedot, Katherine Gurdziel, Susan Irtenkauf, Laura Hasselbach, Yuling Meng, Claudius Mueller, Emanuel F Petricoin, Stephen Brown, Neeraja Purandare, Sidhesh Aras, Tom Mikkelsen, Laila Poisson, Houtan Noushmehr, Douglas Ruden, Ana C deCarvalho

“…We observed that activation of NF-κB was positively correlated with the levels of O-6-methylguanine-DNA methyltransferase (MGMT) protein, a direct DNA repair enzyme leading to TMZ resistance, regardless of MGMT promoter methylation status, further supporting the clinical potential for inhibition of NF-kB signaling in GBM treatment. …”
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Combating antibiotic resistance: mechanisms, challenges, and innovative approaches in antibacterial drug development by Aiswarya M. Rajesh, Shraddha Subhash Pawar, Kruthi Doriya, Rambabu Dandela

“…Bacteria have evolved complex resistance strategies, such as enzyme production to neutralize drugs, modifying drug targets, and using efflux pumps to remove antibiotics, significantly reducing drug efficacy. …”
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Immunosuppressants in modern approaches to treatment of inflammatory bowel diseases by O. B. Schukina, A. G. Kharitonov, A. M. Kharitidis, T. E. Ivashchenko, Yu. A. Nasykhova, T. V. Gabrusskaya, A. Yu. Baranovsky

“…In all other cases (20 patients) allelic variant TPMT*1 with normal enzyme production have been found.Conclusions. There is no need of study for genetic mutations before prescription of thiopurines, however application of effective methods of evaluation of prognosis of bone marrow toxicity development remains an actual issue. …”
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Species Differences in Paraoxonase Mediated Hydrolysis of Several Organophosphorus Insecticide Metabolites by Russell L. Carr, Mary Beth Dail, Howard W. Chambers, Janice E. Chambers

“…Paraoxonase (PON1) is a calcium dependent enzyme that is capable of hydrolyzing organophosphate anticholinesterases. …”
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Hepatitis B e Antigen Seroconversion Is Related with the Function of Dendritic Cells in Chronic Hepatitis B Virus Infection by Chunjing Lin, Hai Zou, Shumin Wang

“…The expressions of surface markers on DC were detected by flow cytometry, and the stimulatory capacity of DC in allogenic mixed leukocyte reaction (MLR) was tested by CCK-8, and the level of cytokines released by DC was analyzed by enzyme-linked immunosorbent assay (ELISA). Results. …”
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A clinical trial evaluates the effect of pomegranate extract as a mouth wash for gingivitis in type 2 diabetic patients by Shayma Ghazi, Dilyar Ahmed Baban, Saif Monem Aziz

“…The aim is to evaluate the efficacy of pomegranate peel extract on the clinical and biochemical measures of (IL-1β) and the enzyme (AST) in saliva of (T2DM) patients suffering from gingivitis in comparison with chlorhexidine. …”
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A Parasite That Should not be Neglected in Patients with Ulcerative Colitis: Entamoeba histolytica by Murat Soylu, Abdurrahman Ekici, Selahattin Aydemir, Şehriban Yürektürk, Önder Akkaş

“…The samples collected were first analyzed macroscopically and then using native-Lugol, trichrome staining, and enzyme-linked immunosorbent assay (ELISA). Results: In the microscopic examination, Entamoeba spp. cysts were found in 22% of the ulcerative colitis patients and 2% of those in the control group. …”
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Pseudogenization of the Slc23a4 gene is necessary for the survival of Xdh-deficient mice by Kazuki Terada, Tamaki Watanabe, Nobuhiro Yasuno, Toshio Ohtsubo, Shigeru Shibata, Kimiyoshi Ichida, Makoto Hosoyamada

“…Hypoxanthine or xanthine levels become excessive and thus toxic in Xdh(− / −) mice because enhancing the activity of hypoxanthine phosphoribosyl transferase (HPRT), which is an enzyme that uses hypoxanthine as a substrate, slightly increases the life span of these mice. …”
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Toxicity of Anethole and the Essential Oils of Lemongrass and Sweet Marigold to the Parasitic Mite Varroa destructor and Their Selectivity for Honey Bee (Apis mellifera) Workers an... by Qodratollah Sabahi, Mollah Md. Hamiduzzaman, Juan S. Barajas-Pérez, Jose M. Tapia-Gonzalez, Ernesto Guzman-Novoa

“…The expression of AChE, a gene that regulates the production of acetyl cholinesterase, a detoxifying enzyme, was not altered in bees treated with the plant compounds at 48 h post-treatment. …”
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IL-17 and IL-22 in Cerebrospinal Fluid and Plasma Are Elevated in Guillain-Barré Syndrome by Shujuan Li, Ming Yu, Haifeng Li, Hongliang Zhang, Yanfang Jiang

“…We hypothesized that interleukin (IL)-17 and IL-22 are elevated in GBS and participate in the autoimmune inflammatory response of GBS. We used sandwich enzyme-linked immunosorbent assay (ELISA) to measure the IL-17 and IL-22 levels in the CSF, and plasma from 22 GBS patients at the acute phase and 18 healthy controls (HC). …”
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Evaluation of the humoral immune responses of brucellosis vaccine (Rev.1) in sheep: A study from Iran by J. Gharekhani, E. Barati, M. Dadar

“…These samples were tested using the modified Rose Bengal plate test (mRBPT), Wright test, 2-mercaptoethanol (2-ME) test, and enzyme-linked immunosorbent assay (ELISA) techniques. …”

Utility of Tetrahydrobiopterin Pathway in the Assessment of Diabetic Foot Ulcer: Significant and Complex Interrelations by Marwan Al-Nimer, Rawa Ratha, Taha Mahwi

“…Serum BH4 markers including NO, Neop, and Biop were determined by using an enzyme-linked immunosorbent assay (ELISA) technology. …”
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Protective Role of rAAV-NDI1, Serotype 5, in an Acute MPTP Mouse Parkinson's Model by Jennifer Barber-Singh, Byoung Boo Seo, Akemi Matsuno-Yagi, Takao Yagi

“…We have recently shown that the non-proton-pumping, internal NADH-ubiquinone oxidoreductase (Ndi1) from Saccharomyces cerevisiae (baker's yeast) can be successfully inserted into the mitochondria of mice and rats, and the enzyme was found to be fully active. Using recombinant adenoassociated virus vectors (serotype 5) carrying our NDI1 gene, we were able to express the Ndi1 protein in the substantia nigra (SN) of C57BL/6 mice with an expression period of two months. …”
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Studies on the preparation of a sufficient carrier from egg protein and carrageenan for cellulase with optimization and application by Marwa I. Wahba, Shireen A. A. Saleh, Walaa A. Abdel Wahab, Faten A. Mostafa

“…It was determined that 60 °C and 2% for free form and 60 °C and 3% for im-cellulase were the optimum temperature and CMC concentration parameters for maximum enzyme activity. Free and im-cellulase were determined to have Km and Vmax of 2.22 mg.ml-1 and 1.76 µmol.ml-1.min-1, and 4.55 mg.ml-1 and 3.33 µmol.ml-1.min-1, respectively. …”
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The Efficacy of On-Site Integration Screening and Microelimination Programs for Chronic Hepatitis C in a Detection Center: A Comparison of the Treatment Outcomes and Characteristic... by Hsuan-Yuan Chang, Su-Hung Wang, Hsing-Tao Kuo, Ming-Jen Sheu, I-Che Feng, Chung-Han Ho, Jui-Yi Chen, Chi-Shu Sun, Chi-Hsing Chen, Cheng-Yi Lin, Chun-Chi Yang

“…However, the patients in the incarcerated group had a significantly different genotype distribution compared to the control group, significantly lower liver enzyme levels, and higher albumin and bilirubin levels compared to those in the control group. …”
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Colorimetric Detection of Dopamine Based on Peroxidase-like Activity of β-CD Functionalized AuNPs by Sara Anderson, Hamish Shepherd, Kiran Boggavarapu, Janak Paudyal

“…Catalytically active nanomaterials, or nanozymes, have gained significant attention as alternatives to natural enzymes due to their low cost, ease of preparation, and enhanced stability. …”
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