Constitutive Expression of Functionally Active Protease-Activated Receptors 1 and 2 in Human Conjunctival Epithelial Cells by Timothy J. Nickel, Mohammad H. Kabir, Jaya Talreja, Daniel J. Stechschulte, Kottarappat N. Dileepan

“…The results show that HCECs constitutively express PAR1 and PAR2 mRNA and proteins, and produce significant amounts of IL-6 when incubated with specific PAR-activating enzymes or agonist peptides. Thrombin- and tryptase-induced HCEC activation was blocked by PAR1 and PAR2 neutralizing antibodies, respectively, and by specific enzyme inhibitors. …”
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Shigellosis Caused by CTX-M Type ESBL Producing Shigella flexneri in Two Siblings of Rural Nepal: First Case Report from the Country by Narayan Prasad Parajuli, Govardhan Joshi, Bashu Dev Pardhe, Jyotsna Shakya, Anjeela Bhetwal, Shreena Shakya, Roshan Pandit, Sumesh Shreekhanda Shrestha, Puspa Raj Khanal

“…In MDR strains, the third-generation cephalosporins have been used for the treatment, but, unfortunately, emerging reports of enzyme mediated β-lactam resistance among Shigella isolates from various parts of the world have greatly compromised the therapy of pediatric dysentery. …”
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High Level Aminoglycoside Resistance and Distribution of Aminoglycoside Resistant Genes among Clinical Isolates of Enterococcus Species in Chennai, India by Elango Padmasini, R. Padmaraj, S. Srivani Ramesh

“…Detection of aminoglycoside modifying enzyme encoding genes (AME) in enterococci was identified by multiplex PCR for aac(6′)-Ie-aph(2′′)-Ia; aph(2′′)-Ib; aph(2′′)-Ic; aph(2′′)-Id and aph(3′)-IIIa genes. 38.2% isolates carried aac(6′)-Ie-aph(2′′)-Ia gene and 40.4% isolates carried aph(3′)-IIIa gene. aph(2′′)-Ib; aph(2′′)-Ic; aph(2′′)-Id were not detected among our study isolates. aac(6′)-Ie-aph(2′′)-Ia and aph(3′)-IIIa genes were also observed in HLAR E. durans, E. avium, E. hirae, and E. gallinarum isolates. …”
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Sustainable Protein Sources: Functional Analysis of <i>Tenebrio molitor</i> Hydrolysates and Attitudes of Consumers in Poland and Spain Toward Insect-Based Foods by Marcelina Maciejewska, Anna Dąbrowska, Marina Cano-Lamadrid

“…The study optimized the process of enzyme hydrolysis using serine protease from <i>Cucurbita ficifolia</i>, thereby enhancing DPPH scavenging capacity increased from 3.15 ± 0.53 to 8.17 ± 0.62 µM Trolox/mL and ABTS decolorization capacity increased from 4.29 ± 0.01 to 10.29 ± 0.01 µM Trolox/mL after 5 h of hydrolysis. …”
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Insights into the mechanism of antiproliferative effects of primaquine-cinnamic acid conjugates on MCF-7 cells by Mabeta Peace, Pavić Kristina, Zorc Branka

“…Further analysis to determine the effects of test compounds on caspase activity and on the levels of apoptosis proteins were undertaken using the enzyme-linked immunosorbent assay (ELISA). Haematoxylin and eosin staining revealed that compounds 1 and 3 induced morphological changes in MCF-7 cells characteristic of apoptosis, while 2-treated cells were in interphase. …”
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Evaluation of Irisin Level in Iraqi Patients with Type 2 Diabetes and Pre-Diabetes Status as a Predictive Factor by Hiba Mohammed Hatem, Kadhim Khudhair Ghudhaib

“…The current study included 138 subjects divided into three groups based on fasting blood glucose (FBG) and glycosylated hemoglobin criteria, including T2DM, 46 patients, pre-diabetes, 46 participants, and healthy subject groups, 46 persons) when they enrolled in the National Diabetes Center/ Al-Mustansiriya University. Enzyme-linked immunosorbent assays were used to detect the levels of irisin and insulin, and spectrophotometric techniques were used to determine the levels of FBG and lipid profile. …”
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GutBugDB: a web resource to predict the human gut microbiome-mediated biotransformation of biotic and xenobiotic molecules by Usha Longwani, Ashok K. Sharma, Aditya S. Malwe, Shubham K. Jaiswal, Vineet K. Sharma

“…It provides information on gut microbiome enzyme-mediated metabolic biotransformation for 1439 FDA-approved drugs and nutraceuticals. …”
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Effect of Ozone Combined with 1-MCP on the Color and Quality of Nectarines during Postharvest Storage by Chen LIU, Yibin ZHANG, Xiaoyu JIA, Zhiyong ZHAO

“…Compared with 1-MCP group alone, 1-MCP group combined with ozone treatment had higher anthocyanin content, centaurin-3-O-glucoside content, centaurin-3-O-rutin content and enzyme activity related to anthocyanin metabolism at the later stage of storage. …”
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Acute Kidney Injury Secondary to Necrotizing Sarcoid Granulomatosis by Varun Mamidi, Manikantan Shekar, Jaiju James Chakola, Vamsi Krishna Makkena, Jayakumar Matcha

“…All the markers of tuberculosis were negative and angiotensin converting enzyme levels were elevated. Patient improved with 1 mg/kg/day oral steroid therapy and is on regular follow-up with minimal dose of steroids. …”
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Intracellular Shuttle: The Lactate Aerobic Metabolism by Rogério Santos de Oliveira Cruz, Rafael Alves de Aguiar, Tiago Turnes, Rafael Penteado Dos Santos, Mariana Fernandes Mendes de Oliveira, Fabrizio Caputo

“…A new hypothesis has been presented based on the fact that lactate-to-pyruvate conversion cannot occur in cytosol, because the LDH enzyme characteristics and cytosolic environment do not allow the reaction in this way. …”
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Peroxidase-like activity of citrate-functionalized magnetite nanoparticles augmented its antibacterial potency by Haitham Al-Madhagi, Abd Alraouf Tarabishi, Fatima Al-Zahra Hamsho, Zaher Tahan, Lama Alchab, Wassim Abdelwahed

“…The classic utilization of nanomaterials has now transformed to serve enzyme-like activities (nanozymes) that found different applications, particularly as antibacterial agents. …”
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Self‐Driving Lab for Solid‐Phase Extraction Process Optimization and Application to Nucleic Acid Purification by Sebastian Putz, Jonathan Döttling, Tim Ballweg, Andre Tschöpe, Vitaly Biniyaminov, Matthias Franzreb

“…Sorptive bioprocesses are the basis for numerous biotechnological applications such as enzyme immobilization, biosensors, controlled drug delivery, water treatment, and molecular purification. …”
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Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments by José A. O'Daly, Humberto M. Spinetti, Joe Gleason, María B. Rodríguez

“…Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P<0.001), with a diagnostic value from 1.50 to 1.84. …”
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Exogenous dopamine application ameliorates chilling injury and preserves quality of kiwifruit during cold storage by Morteza Soleimani Aghdam, Zeinab Asle-Mohammadi, Amin Ebrahimi, Farhang Razavi

“…In addition, higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity arising from higher phenols and flavonoids accumulation in kiwifruits treated with 150 µM dopamine could be ascribed to higher phenylalanine ammonia-lyase (PAL) enzyme activity. Additionally, lower endogenous hydrogen peroxide (H2O2) accumulation along with higher ascorbic acid accumulation in kiwifruits treated with 150 µM dopamine could be attributed to lower superoxide dismutase (SOD) along with higher catalase (CAT) enzymes activity. …”
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Molecular, Biochemical, and Clinical Characterization of Thirteen Patients with Glycogen Storage Disease 1a in Malaysia by Siti Aishah Abdul Wahab, Yusnita Yakob, Mohd Khairul Nizam Mohd Khalid, Noraishah Ali, Huey Yin Leong, Lock Hock Ngu

“…GSD1a is caused by a mutation in the G6PC gene encoding glucose-6-phosphatase (G6Pase); an enzyme that catalyses the hydrolysis of glucose-6-phosphate (G6P) to phosphate and glucose. …”
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Metabolic pathways of eicosanoids—derivatives of arachidonic acid and their significance in skin by Michał Biernacki, Elżbieta Skrzydlewska

“…Phospholipids are subject to continuous transformation, including enzymatic hydrolysis (with the participation of phospholipases A, C, and D) to free polyunsaturated fatty acids (PUFAs), which under the influence of cyclooxygenases (COX1/2), lipoxygenases (LOXs), and cytochrome P450 (CYPs P450) are metabolized to various classes of oxylipins, depending on the type of PUFA being metabolized and the enzyme acting. The most frequently analyzed oxylipins, especially in skin cells, are eicosanoids, which are derivatives of arachidonic acid (AA). …”
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Network Pharmacology Analysis and Experimental Validation to Explore the Anti-inflammatory Mechanism of Asiatic Acid on Alcoholic Steatohepatitis by Tao Jiang, Jinhong Xu, Yang Lu, Xiaojin Chen, Yongxia Li

“…In addition, in the cellular model, real-time reverse transcriptase-polymerase chain reaction (Real-Time RT-PCR) and enzyme-linked immunosorbent assay (ELISA) results showed that AA could alleviate alcohol-induced cellular inflammation, while western blot and immunofluorescence results showed that AA might alleviate alcohol-induced cellular inflammation by inhibiting the nuclear factor-κB (NF-κB) pathway. …”
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One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal by Juliana R. Machado, Emanuelle E. Severo, Janaina M. G. de Oliveira, Joana da C. Ores, Adriano Brandelli, Susana J. Kalil

“…The one-step ultrafiltration process with the membrane molecular mass cutoff of 10 kDa at pH 8.0 and operating pressure of 0.147 MPa showed an enzyme recovery of 87.8% and a 4.1-fold purification factor. …”
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Autoantibody Response to Murine Double Minute 2 Protein in Immunodiagnosis of Hepatocellular Carcinoma by Mei Liu, Su-jun Zheng, Yu Chen, Ning Li, Peng-fei Ren, Li-ping Dai, Zhong-ping Duan, Jian-Ying Zhang

“…In this study, autoantibody responses to MDM2 protein in HCC patient’s serum were evaluated by enzyme-linked immunosorbent assay (ELISA) and part sera were evaluated by Western blotting and indirect immunofluorescence assay. …”
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The Effects of Laurencia caspica Algae Extract on Hemato-Immunological Parameters, Antioxidant Defense, and Resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis... by Majid Khanzadeh, Babak Beikzadeh, Seyed Hossein Hoseinifar

“…In addition, feeding with L. caspica significantly increased the antioxidant enzyme activities on the 25th day (L. caspica 1% and 2% in peroxidase [POD] and superoxide dismutase [SOD] in all groups) and 50th day (catalase [CAT], SOD and L. caspica 1% and 2% in POD) in the spleen. …”
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