Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography by Marius Schmidt

“…To start a reaction in an enzyme, typically, an initially inactive substrate present in the crystal is activated. …”
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A Computational Approach towards the Development of Newer Anticancer Agents by Javed I. Sheikh, H. D. Juneja, V. N. Ingle

“…In silico molecular docking analyses of the novel 1-(2', 4'-dihydroxy-5'-chlorophenyl)-3-aryl-propane-1, 3-diones were performed in the active sites of enzyme cytochrome P450 reductase to obtain new anticancer agents computationally. 1-(2', 4'-dihydroxy-5'-chlorophenyl)-3-(4"-methoxy-phenyl)-propane-1, 3-dione (3f) was found to have maximum affinity for the active sites of enzyme.…”
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m6A demethylase CpALKBH regulates CpZap1 mRNA stability to modulate the development and virulence of chestnut blight fungus by Lijiu Zhao, Xiangyu Wei, Fengyue Chen, Baoshan Chen, Ru Li

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The Effect of Clopidogrel and Ticagrelor on Human Adipose Mesenchymal Stem Cell Osteogenic Differentiation Potential: In Vitro Comparative Study by Sally S. Mohamed, Hala F. Zaki, Shereen N. Raafat

“…In comparison to all study groups, the TICA group showed significant increase in the mineralized extracellular matrix, ALP enzyme activity, and bone markers expression as RUNX2 (P<0.0001), OC, and OPG (P<0.05). …”
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Enhanced Production and Characterization of a Solvent Stable Amylase from Solvent Tolerant Bacillus tequilensis RG-01: Thermostable and Surfactant Resistant by Soni Tiwari, Neha Shukla, Pooja Mishra, Rajeeva Gaur

“…The enzyme was also stable in the presence of SDS, Tween-40, Tween-60, and Tween-80 (1%) and was found stimulatory effect, respectively. …”
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Glycogen Storage Disease Type IX in a 6-year-old Male: A Case Report by Shahab Noorian, Hossein Moravej, Zhila Afshar, Afagh Hassanzadeh Rad, Setila Dalili

“…Background: Glycogen storage diseases (GSDs) are a group of inherited metabolic disorders caused by enzyme deficiencies affecting glycogen synthesis or breakdown. …”
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Characterization of the algC Gene Expression Pattern in the Multidrug Resistant Acinetobacter baumannii AIIMS 7 and Correlation with Biofilm Development on Abiotic Surface by Praveen K. Sahu, Pavithra S. Iyer, Sagar H. Barage, Kailas D. Sonawane, Balu A. Chopade

“…Cloning, heterologous expression, and bioinformatics analyses indicated algC gene product as the bifunctional enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) of ∼53 kDa size, which augmented biofilms significantly in algC clones compared to controls (lacking algC gene), further localized by scanning electron microscopy. …”
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Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries by Hameed Abdul, Akhtar Naveed

“…The highest inhibition of tyrosinase enzyme was also exhibited by WSEA and SNEA (59.6 and 58.7 %) resp. …”
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Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells by Zhongjun Wan, Shanshan Wen, Ran Zheng, Li Li, Wei Jiang, Donghui Zhang

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Altered Thyroid Function Tests Observed in Hypophosphatasia Patients Treated with Asfotase Alfa by Hajime Kato, Naoko Hidaka, Minae Koga, Yuka Kinoshita, Masaomi Nangaku, Noriko Makita, Nobuaki Ito

“…After the initiation of asfotase alfa, three HPP patients showed low free triiodothyronine (FT3) and free thyroxine (FT4) measured with AIA-2000 (Tosoh, Tokyo, Japan), an enzyme immunoassay system that uses ALP as a labeling enzyme, but their thyroid-stimulating hormone (TSH) levels were within the normal range. …”
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Plant and marine-derived natural products: sustainable pathways for future drug discovery and therapeutic development by Muhammad Ahmad, Muhammad Ahmad, Maleha Tahir, Zibin Hong, Muhammad Anjum Zia, Hamza Rafeeq, Muhammad Shaheez Ahmad, Saif ur Rehman, Junming Sun

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Full-length inhibitor protein is the most effective to perturb human dUTPase activity by Bianka Kőhegyi, Zoé S. Tóth, Enikő Gál, Máté Laczkovich, András Benedek, Beáta G. Vértessy, Kinga Nyíri

“…Abstract It has been demonstrated recently that knockout of the dUTPase enzyme leads to early embryonic lethality in mice. …”
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NMR, Novel Pharmacological and In Silico Docking Studies of Oxyacanthine and Tetrandrine: Bisbenzylisoquinoline Alkaloids Isolated from Berberis glaucocarpa Roots by Muhammad Alamzeb, Muhammad Omer, Mamoon Ur-Rashid, Muslim Raza, Saqib Ali, Behramand Khan, Asad Ullah

“…Urease enzyme is responsible for gastric cancer, peptic ulcer, hepatic coma, and urinary stones in millions of people across the world. …”
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Biosensor Based on the Immobilization of Laccase on β‐Cyclodextrin Membrane for the Evaluation of Antioxidant Capacity in Real Samples by Jorge Juárez‐Gómez, Omar Alejandro Báez‐Melga, Dafne Sarahia Guzmán‐Hernández

“…The process of electropolymerization, which was successful even in the presence of the enzyme, was a key step in biosensor synthesis. Variables such as pH, temperature, and enzyme concentration were optimized using a factorial design with two levels for each factor. …”
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Antioxidant capacity in leaves of pear cultivars during the summer season by A. l. Mishko, N. V. Mozhar, A. V. Klyukina, V. V. Vyalkov

“…The control European cultivar ‘Williams’ had a lower level of enzyme activity in the summer of 2021, compared to the Russian cultivars ‘Lyuberskaya’ and ‘Flamenco’. …”
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Optimized extraction and kinetic study of cholesterol oxidase from a newly isolated Escherichia fergusonii strain from local whey samples: insights through a combined experimental... by Simin Khataee, Gholamreza Dehghan, Samaneh Rashtbari, Arezu Marefat, Sina Jamei, Hamed Farzi-Khajeh

“…Furthermore, the ChoX enzyme revealed acceptable stability at a pH value of 6.5 to 8.5, maintaining its initial activity of more than 50.0%. …”
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Enhancements of soil priming effect and carbon sequestration in degraded alpine meadows via supplementation: a comparative study of Tibetan sheep (Ovis aries) and plateau pika (Och... by Qinyao Li, Wenquan Yang, Yanru Wu, Xilai Li, Jiancun Kou, Yanghua Yu, Yangcan Zhang, Xinting He, Yunlong Zhao

“…In particular, TN directly negatively affected PE, SOC and the NO3 −-N:NH4 +-N ratio indirectly affected PE by affecting soil enzyme activity and MBC. Conclusions This study demonstrates that the supplement of animal excrements can improve the extracellular enzyme activity and nitrogen content in soils, which is conducive to PE and net carbon balance. …”
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Effects of the Treatment Technology of Soybean on Volatile Flavor Compounds of Plant-based Compound Milk during Storage by Xue WANG, Youqiang YU, Xiaoyang ZHENG, Hongjiang MA, Shiwei LIU, Wenxin WANG, Fang ZHOU, Shenglin DUAN

“…Headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) combined with electronic nose and human sensory evaluation were used to analyze the differences of volatile flavor substances in the plant-based compound milk, that prepared by two soybean treatment technologies (oxygen separation grinding/enzyme inactivation with high temperature, grinding after enzyme inactivation) at different storage time (0, 90, 180 and 360 d). …”
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L-glutaminase synthesis by Klebsiella pneumoniae (AS KP 23) isolated from clinical strain, and its efficacy against human hepatocellular and breast cancer cell lines by Lina J. Abdel-Hafez, Eman Y. T. Elariny, Asmaa E. Ibrahim, Mahmoud E. F. Abdel-Haliem

“…Further kinetic studies on the purified enzyme were performed. In addition, the pH stability of the L-glutaminase enzyme was slightly affected over the pH range of 7.0–9.0 after 2 h of pre-incubation, and the rate of thermal inactivation of the L-glutaminase enzyme increased with higher temperatures and longer preheating periods. …”
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Evaluation of in-vitro and in-silico antidiabetic potential, glucose metabolism, antioxidant, cytotoxicity and phytochemical content of Ipomoea bolusiana Schinz and Ipomoea crassip... by Garland Kgosi More, Nolitha Nkobole, Charmy Twala, Sechaba Machedi, Tebogo Amos Moswetsa, Ramakwala Christinah Chokwe

“…Methods: In vitro antidiabetic activity was tested with α-amylase enzyme inhibition method and antioxidant activity was evaluated. …”
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