Metabolic engineering approaches for the biosynthesis of antibiotics by Geunsoo Yook, Jiwoo Nam, Yeonseo Jo, Hyunji Yoon, Dongsoo Yang

“…To address this challenge, metabolic engineering has recently become crucial in developing efficient enzymes and cell factories capable of producing both existing antibiotics and a wide range of new derivatives and analogs. …”
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Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains of Bacillus subtilis in Submerged and Solid State Fermentatio... by Hamid Mukhtar, Ikramul Haq

“…In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease by Bacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. …”
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Antibody to Hepatitis C Virus in Risk Groups in Canada by RK Chaudhary, Theresa Mo

“…The number of samples positive for antibodies to HCV (anti-HCV) was higher with enzyme immunoassay than by RIBA HCV in most cases. …”
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Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay by Felipe Antunes Calil, Juliana Maria Lima, Arthur Henrique Cavalcante de Oliveira, Christiane Mariotini-Moura, Juliana Lopes Rangel Fietto, Carmen Lucia Cardoso

“…The use of IMERs (Immobilized Enzyme Reactors) as a stationary phase coupled to high performance chromatographic systems is an interesting approach in the screening of new ligands. …”
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Alzheimer-mutant γ-secretase complexes stall amyloid β-peptide production by Parnian Arafi, Sujan Devkota, Emily Williams, Masato Maesako, Michael S Wolfe

“…We recently reported evidence that FAD mutations in APP and PSEN1 cause deficiencies in early steps in processive proteolysis of APP substrate C99 and that this results from stalled γ-secretase enzyme-substrate and/or enzyme-intermediate complexes. …”
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Investigating the impact of Multiwalled Carbon Nanotubes exposure on enzymatic activities and histopathological variations in Swiss albino mice by Iqra Nasim, Nadia Ghani, Rab Nawaz, Emilio Mateev, Yousef A. Bin Jardan

“…Serum samples were extracted at 7 and 14 days after the experiment by centrifuging whole blood for 15 min at 3,000 rpm. An enzyme-linked immunosorbent test (ELISA) was used to measure many enzyme assays, such as Angiotensin Converting Enzymes (ACE), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), and Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase enzyme. …”
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DICER1: The Argonaute Endonuclease Family Member and Its Role in Pediatric and Youth Pathology by Consolato M. Sergi, Fabrizio Minervini

“…In 2001, two enzyme-encoding genes were recognized in the fruit fly <i>Drosophila melanogaster</i>. …”
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An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids by Suleyman Aydin, Elif Emre, Kader Ugur, Mustafa Ata Aydin, İbrahim Sahin, Vedat Cinar, Taner Akbulut

“…The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. …”
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The protein interactome of Escherichia coli carbohydrate metabolism. by Shomeek Chowdhury, Stephen S Fong, Peter Uetz

“…We specifically investigated the stoichiometry of 378 PPIs involving carbohydrate metabolic enzymes. In 48 interactions, the interactors were much more abundant than the enzyme and are thus likely to affect enzyme activity and carbohydrate metabolism. …”
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Perioperative infarction during coronary bypass surgery: an attempt to refine the diagnostic criteria using data from a retrospective case–control study by Lars Niclauss, Liam-Kani Roulet, Piergiorgio Tozzi, Filip Dulguerov, Ziyad Gunga, Anna Nowacka, Matthias Kirsch

“…Objective The definition of coronary artery bypass graft (CABG)-associated myocardial infarction (MI) is controversial because the postoperative increases in cardiac enzyme activities are multifactorial in origin. Methods We performed a retrospective case–control study of patients who experienced perioperative MI (cardiac enzyme release, electrocardiographic changes, dysfunction on echocardiography) and those without ischemia to identify risk factors and enzyme activity thresholds. …”
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Immobilization of α-Chymotrypsin on the Surface of Magnetic/Gold Core/Shell Nanoparticles by Mahmoud Kamal Ahmadi, Manouchehr Vossoughi

“…FTIR and fluorescence spectroscopy were utilized to study secondary and tertiary structures of the immobilized enzyme. Results show that the secondary and tertiary structures of the enzyme remain virtually unchanged after immobilization on the nanoparticles surface. …”
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Biochemical responses of painted spiny lobster Panulirus versicolor fed with molluscs meal and fish meal by Agus Kurnia, Muhaimin Hamzah, Muhammad Idris, Wellem Henrik Muskita, La Ode Baytul Abidin, Kadir Sabilu, Yusnaini Yusnaini, Abdul Muis Balubi, La Usaha La Usaha, Famawati Fatmawati, La Ode Abdul Razak, Khairunnisa Khairunnisa

“…The results showed that the enzyme activities of lobsters fed with combined mollusc meals were higher than the enzyme activity in the lobsters fed with the fish meal combination diet. …”
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A one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers by Li Chen, Yunrong Li, Xuanxuan Zhang, Lixin Ma, Cheng Zhang, Huanhuan Chen

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Conformation and Catalytic Properties Studies of Candida rugosa Lip7 via Enantioselective Esterification of Ibuprofen in Organic Solvents and Ionic Liquids by Xiang Li, Shuangshuang Huang, Li Xu, Yunjun Yan

“…Enantioselective esterification of ibuprofen was conducted to evaluate the enzyme activity and ees of lipase from Candida rugosa (CRL7) in ten conventional organic solvents and three ionic liquids. …”
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Effect of pH on the Hydrolytic Kinetics of Gamma-Glutamyl Transferase from Bacillus subtilis by Sharath Balakrishna, Asmita Prabhune

“…We also analysed the effect of pH on the structure of the enzyme. The circular dichroism spectra of the enzyme sample at pH 9.0 and 11.0 were coincidental in both far and near UV regions indicating conservation of the secondary and tertiary structures, respectively. …”
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Bubbling insights: unveiling the true sophorolipid biosynthetic pathway by Starmerella bombicola by Sophie L. K. W. Roelants, Stijn Bovijn, Elvira Bytyqi, Nicolas de Fooz, Goedele Luyten, Martijn Castelein, Thibo Van de Craen, Zhoujian Diao, Karolien Maes, Tom Delmulle, Maarten De Mol, Sofie L. De Maeseneire, Bart Devreese, Wim K. Soetaert

“…These results indicate that the AT enzyme is not the only enzyme responsible for acetylation of sophorolipids, while the SBLE enzyme performs an intramolecular transesterification reaction on bolaform glycolipids instead of an esterification reaction on acidic sophorolipids. …”
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Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease by Tanveer Majeed, Romana Tabassum, William J. Orts, Charles C. Lee

“…In addition, the enzyme had an elevated optimum temperature (60°C). …”
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Biodesulfurization enhancement via targeted re-insertion of the flavin reductase dszD in the genome of the model strain Rhodococcus qingshengii IGTS8 by Olga Martzoukou, Fotios Klenias, Eleni Kopsini, Dimitris G. Hatzinikolaou

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A comprehensive in silico analysis of structural and functional impacts of natural nonsynonymous SNPs in the ALDH2_HUMAN gene by Mehr Ali Mahmood Janlou

“…Mitochondrial aldehyde dehydrogenase 2 (ALDH2) is a key enzyme involved in the metabolism of acetaldehyde to acetate through a coenzyme-dependent mechanism. …”
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Structural Characterization of Functional Ingredient Levan Synthesized by Bacillus siamensis Isolated from Traditional Fermented Food in Thailand by Nattapong Thakham, Supphasin Thaweesak, Nuttinee Teerakulkittipong, Natthiwut Traiosot, Autaipohn Kaikaew, Gary Antonio Lirio, Witawat Jangiam

“…To address the problem, the present work is aimed at isolating levansucrase enzyme-producing microorganisms from traditional fermented food in Thailand. …”
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