Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean by Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith

“…Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence‐specificity, careful design of the editing reagents can also reduce unintended edits at highly homologous sites. …”
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Research note: Increased lipid accumulation within broiler preadipocytes during differentiation in vitro at atmospheric oxygen tension by Jéssica Moraes Cruvinel, Elizabeth S. Greene, Rachel W. Read, Kichoon Lee, Sami Dridi, Paula R. Chen

“…Abundance of glutathione peroxidase 4 (GPX4) was increased in all the differentiation cultures compared to the controls, regardless of oxygen tension; however, differences in the abundance of other antioxidant enzymes were not observed. Overall, exposure to atmospheric oxygen tension promotes lipid accumulation within chicken preadipocytes, which may need to be considered when developing in vitro models of this process.…”
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Multi-stage structure-based virtual screening approach combining 3D pharmacophore, docking and molecular dynamic simulation towards the identification of potential selective PARP-1... by Mahmoud A. El Hassab, Wagdy M. Eldehna, Ghaneya S. Hassan, Sahar M. Abou-Seri

“…Abstract Presently, humanity is confronted with a range of diseases that have high death rates, especially those linked to cancerous growths. Several enzymes and proteins have been discovered as highly attractive targets for cancer treatment. …”
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Split Application of Potassium Reduces Rice Chalkiness by Regulating Starch Accumulation Process Under High Temperatures by Xinyue Zhang, Youfa Li, Junjie Dong, Yuanze Sun, Haowei Fu

“…Similarly, the gene expression levels of enzymes involved in this process were decreased by 3.52–24.12% at 5 DAA but increased by 8.61–30.00% at 20 DAA by the split application of K fertilizer. …”
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Molecular Epidemiology and Antimicrobial Resistance of Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella pneumoniae in Retail Cattle Meat by Nasrin Akter Liza, Hemayet Hossain, Md. Shahidur Rahman Chowdhury, Jarin Al Naser, Rayhan Mahmud Lasker, Asikur Rahman, Md. Ariful Haque, Md. Al Mamun, Md. Mukter Hossain, Md. Mahfujur Rahman

“…Antimicrobial susceptibilities were assessed using disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI, 2020) guidelines. Genes encoding ESBL enzymes were detected by the double-disk synergy test (DDST) and multiplex PCR. …”
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GENOME POLITICS: STRATEGY AND TACTICS by Jonas Rubikas

“…This is clearly seen when the comparison between the information content (the gene number) and the life cycle of small genomes (plasmids, viruses, singlecellular prokaryotic organisms) is made: large space is occupied by information on replication enzymes and the sequence of replication origin. In higher eukaryotic multicellular organisms the increased complexity of the genome could be expressed as plus function information: for metabolism, for relations with environment, for nervous system. …”
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Hemoprotein dalam Tubuh Manusia by Husnil Kadri

“…Base on Fe ion state in heme group, hemoproteins are divided into: (1) Hemoproteins have ion Fe2+ state that can bind oxygen, such as hemoglobin, myoglobin, neuroglobin, and cytoglobin. (2) Hemoproteins have ion Fe3+ state that act as oxidoreductase enzymes, such as cytochrome P450, cytochromes in oxidative phosphorylation, catalase, tryptophan pyrolase, and NO synthase. …”
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Endophytic strategies decoded by genome and transcriptome analysis of Fusarium nematophilum strain NQ8GII4 by Siyuan Yan, Qingchen Zhang, Shuxin Jia, Miaomiao Guo, Qiangqiang Zhang, Peiwen Gu, Peiwen Gu

“…During the early stages of symbiosis establishment, genes encoding glycosyltransferases (GTs), fungal cell wall-degrading enzymes (FCWDEs), and steroid-14α-demethylase (CYP51) were significantly downregulated, potentially suppressing hyphal growth of the fungus. …”
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Physalis Calyx seu Fructus relieves chicken intestinal damage to heat via improving the antioxidant ability by Bin Yin, Changning Juan, Rongling Zhang, Shifa Yang, Haiting Wang, Yueyue Liu, Shikai Song, Zunxiang Yan, Yunpeng Yi, Zengcheng Zhao, Zhongli Huang, Shuqian Lin

“…The mitigating effect was assessed through the oxidation-related enzymes and key genes, histopathology, and inflammatory factors. …”
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Phenotypic Profiling of Selected Cellulolytic Strains to Develop a Crop Residue-Decomposing Bacterial Consortium by Arman Shamshitov, Egidija Satkevičiūtė, Francesca Decorosi, Carlo Viti, Skaidrė Supronienė

“…However, this solution often focuses mostly on selecting microorganisms based on the appropriate enzymic capabilities and neglects the metabolic versatility required to utilize both structural and non-structural components of residues. …”
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Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal by Roshan Pandit, Balkrishna Awal, Sumesh Shreekhanda Shrestha, Govardhan Joshi, Basista Prasad Rijal, Narayan Prasad Parajuli

“…Escherichia coli (n = 154), the predominant pathogen associated with UTI, was further investigated for the existence of ESBL enzymes by using conventional phenotypic as well as molecular approaches. …”
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Impact of strict IGF1 control on quality-of-life scores in patients with acromegaly by Chaitanya Gandhi, Marie-Claire Denis, Daniel Holmes, Juan Rivera, Stan van Uum, Shereen Ezzat, Constance Chik

“…Hemoglobin A1c and liver enzymes did not differ and repeat MRI of the sella at 6 months showed no change.ConclusionsIn this pilot study, stricter control of modest IGF1 elevations led to symptomatic improvement as measured by the PASQ score. …”
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High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas by Shiwani Singh, Maneesh S. Bhandari, Neeraj Dwivedi

“…Conclusion: This standardised buffer condition and protocol led to high-quality DNA isolation, which was found good for restriction digestion using endonuclease enzymes, PCR and other molecular biology techniques, highlighting the ongoing efforts to enhance the reliability of genetic studies in medicinal plants. …”
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Tetrahydrobiopterin as a rheostat of cell resistance to oxidant injury by R. Steven Traeger, James Woodcock, Sidhartha Tan, Zhongjie Shi, Jeannette Vasquez-Vivar

“…The relative efficiency of oxidant species at causing variations in BH4/BH2 levels in cells furnished with several antioxidant enzymes has not yet been systematically analyzed. …”
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L-Borneol 7-O-[β-D-Apiofuranosyl-(1→6)]-β-D-Glucopyranoside Alleviates Myocardial Ischemia-Reperfusion Injury in Rats and Hypoxic/Reoxygenated Injured Myocardial Cells via Regulati... by Ziyi Tong, Gaowen Li, Chengxiao Su, Liyan Zhou, Ling Zhang, Qun Chen, Qing Xia

“…The influences of LBAG on the content concentration of cardiac enzymes in the serum were measured by ELISA. Moreover, H9c2 cells were exposed to LBAG or combined with AKT inhibitor (perifosine) and then exposed to H/R for simulating the cardiac injury process. …”
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Phytotoxic Effects of Polystyrene Microplastics on Growth Morphology, Photosynthesis, Gaseous Exchange and Oxidative Stress of Wheat Vary with Concentration and Shape by Komal Riaz, Tahira Yasmeen, Kotb A. Attia, Itoh Kimiko, Muhammad Saleem Arif

“…Interestingly, increasing the PS concentration tended to up-regulate the activity of antioxidant enzymes for all shapes, indicating potential complexity and a highly time-dependent response related to various reactive oxygen species (ROS). …”
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Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury by Farhin Patel, Kirti Parwani, Dhara Patel, Palash Mandal

“…In vivo, combined administration of probiotic V and Met ameliorated the histopathological changes, inflammatory response, hepatic markers (liver enzymes), and lipid metabolism induced by ethanol. …”
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Sarco(endo)plasmic Reticulum Ca2+-ATPase-2 Gene: Structure and Transcriptional Regulation of the Human Gene by Angel Zarain-Herzberg, Georgina Alvarez-Fernandez

“…The sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs) belong to a family of active calcium transport enzymes encoded by the SERCA1, 2, and 3 genes. In this study, we describe the complete structure of the human SERCA2 gene and its 5’ -regulatory region. …”
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Unraveling the Beneficial Role of Resveratrol in Fructose-Induced Non-Alcoholic Steatohepatitis with a Focus on the AMPK/Nrf2 Signaling Axis by Soha S. Zakaria, Safaa M. Hanafy

“…We performed histological evaluations and assessed blood lipids and liver enzymes to study resveratrol’s impact on NASH. Quantitative real-time PCR was used to assess the mRNA expression of nuclear factor E2-related factor 2 (Nrf2) in the liver samples. …”
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Impact of ZnO nanopriming on physiological and biochemical traits of wheat (Triticum aestivum L.) seedling by Priyanka Pandya, Sushil Kumar, Ghanshyam Patil, Monil Mankad, Zarna Shah

“…Additionally, the presence of ZnO NPs led to increased activity of antioxidant enzymes like superoxide dismutase (SOD) and catalase in a dose-dependent mananer. …”
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