Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo by A. A. Lebedeva, N. S. Zakharchenko, E. V. Trubnikova, O. A. Medvedeva, T. V. Kuznetsova, G. A. Masgutova, M. V. Zylkova, Y. I. Buryanov, A. S. Belous

“…It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. …”
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Unseen Enemy: Mechanisms of Multidrug Antimicrobial Resistance in Gram-Negative ESKAPE Pathogens by Giedrė Valdonė Sakalauskienė, Lina Malcienė, Edgaras Stankevičius, Aurelija Radzevičienė

“…Key bacterial mechanisms contributing to resistance include limitations in drug uptake, production of antibiotic-degrading enzymes, alterations in drug target sites, and enhanced drug efflux systems. …”
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Cost-effective whole-cell biosynthesis of ursodeoxycholic acid using engineered Escherichia coli with a multienzyme cascade by Xue Zhang, Xue Zhang, Jiagang Xin, Mengyu Liu, Mengyu Liu, Yue Zhang, Yue Zhang, Haoni Luan, Haoni Luan, Wei Feng, Wei Feng, Fei Wang, Fei Wang, Wei Xu, Wei Xu, Peng Song, Peng Song

“…This strain coexpressed four enzymes under the control of three independent T7 promoters: lactate dehydrogenase (LDH) derived from Lactobacillus delbrueckii, glucose dehydrogenase (GDH) derived from Priestia megaterium, 7α-HSDH derived from E. coli, and 7β-HSDH derived from Ruminococcus torques, enabling the whole-cell catalytic synthesis of UDCA from CDCA. …”
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Mechanistic insight into human milk extracellular vesicle‐intestinal barrier interactions by Xiang Luo, Yunyue Zhang, Ning Ding, Jana Javorovic, Bahijja Tolulope Raimi‐Abraham, Steven Lynham, Xiaoping Yang, Natalie Shenker, Driton Vllasaliu

“…Here, we studied the interaction of human milk EVs with the gut barrier components, including intestinal biofluids, enzymes, mucus and epithelium. Additionally, we probed the endocytic mechanisms mediating the EV intestinal uptake. …”
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Oxidative Stress in Diabetic Nephropathy with Early Chronic Kidney Disease by Alejandra Guillermina Miranda-Díaz, Leonardo Pazarín-Villaseñor, Francisco Gerardo Yanowsky-Escatell, Jorge Andrade-Sierra

“…The overproduction of ROS diminishes expression of the antioxidant enzymes (manganese superoxide dismutase, glutathione peroxidase, and catalase). …”
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<i>Cuphea hookeriana</i>: Phytochemical Profile and the Cosmeceutical and Dermatological Properties of Its Active Fraction from the Whole Plant by Xing Wu, Meng-Fei Wanyan, Bao-Bao Shi, Rong Huang, Hui-Xiang Yang, Xian Wang, Ji-Kai Liu

“…It inhibited the two target enzymes (tyrosinase and MMP-1) engaged in skin whitening and aging with comparable IC₅₀ values to the reference drugs. …”
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Mesenchymal Stem Cells Ameliorate Hepatic Ischemia/Reperfusion Injury via Inhibition of Neutrophil Recruitment by Shihui Li, Xu Zheng, Hui Li, Jun Zheng, Xiaolong Chen, Wei Liu, Yan Tai, Yingcai Zhang, Genshu Wang, Yang Yang

“…Treatment with MSCs protected rat against hepatic IRI, with significantly decreased serum levels of liver enzymes, attenuated hepatic neutrophil infiltration, reduced expression of apoptosis-associated proteins, and ameliorated liver pathological injury. …”
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IL-7 promotes integrated glucose and amino acid sensing during homeostatic CD4+ T cell proliferation by Seema Bachoo, Nancy Gudgeon, Rebecca Mann, Victoria Stavrou, Emma L. Bishop, Audrey Kelly, Alejandro Huerta Uribe, Jordan Loeliger, Corina Frick, Oliver D.K. Maddocks, Paul Lavender, Christoph Hess, Sarah Dimeloe

“…Clinically, CD4+ T cells from stem cell transplant recipients demonstrate coordinated upregulation of glycolytic and TCA cycle enzymes, amino acid-sensing machinery, and mTOR targets, highlighting the potential to therapeutically target this pathway to fine-tune lymphopenia-induced T cell proliferation.…”
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CryoEM structure and small-angle X-ray scattering analyses of porcine retinol-binding protein 3 by Vineeta Kaushik, Luca Gessa, Nelam Kumar, Matyáš Pinkas, Mariusz Czarnocki-Cieciura, Krzysztof Palczewski, Jiří Nováček, Humberto Fernandes

“…The enzymatic isomerization of at-ROL, crucial for restoring the visual pigments and preparing them to receive new light stimuli, relies on various enzymes found in both the photoreceptors and retinal pigment epithelium cells. …”
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Matrix Metalloproteinases: Inflammatory Regulators of Cell Behaviors in Vascular Formation and Remodeling by Qishan Chen, Min Jin, Feng Yang, Jianhua Zhu, Qingzhong Xiao, Li Zhang

“…Matrix metalloproteinases (MMPs), well-known inflammatory mediators are a family of zinc-dependent proteolytic enzymes that degrade various components of ECM and non-ECM molecules mediating tissue remodeling in both physiological and pathological processes. …”
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The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and me... by Jinbin Hao, Liyun Ye, Guoliang Meng, Yanjiao Song, Junsheng Fu, Xiaoping Wu

“…Assessment of the changes in gene expression and metabolites revealed the significantly different expression of several genes encoding key enzymes and metabolites in choline metabolism pathway. …”
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Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials by Alejandro Victoria-Escandell, José Santiago Ibañez-Cabellos, Sergio Bañuls-Sánchez de Cutanda, Ester Berenguer-Pascual, Jesús Beltrán-García, Eva García-López, Federico V. Pallardó, José Luis García-Giménez, Antonio Pallarés-Sabater, Ignacio Zarzosa-López, Manuel Monterde

“…Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. …”
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Impact of Low Light on Photosynthetic Characteristics, Antioxidant Activity, and Yield of <i>Brassica napus</i> L. by Yue Hu, Hafiz Hassan Javed, Li Liu, Yalong Liu, Xiaohui Yang, Feifei Xu, Ying Liu, Xiao Peng, Yongcheng Wu

“…According to the study results, low light reduces key photosynthetic enzymes and sucrose synthase activity in rapeseed leaves, leading to a decrease in leaf carbohydrate accumulation. …”
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Reversible Myocarditis after Black Widow Spider Envenomation by Tarek Dendane, Khalid Abidi, Naoufel Madani, Asmae Benthami, Fatima-Zohra Gueddari, Redoune Abouqal, Amine-Ali Zeggwagh

“…The plasma concentrations of cardiac enzymes were elevated. His first echocardiography showed hypokinesis of the left ventricle (left ventricle ejection fraction 48%). …”
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Antioxidant and Chelating Activity of Nontoxic Jatropha curcas L. Protein Hydrolysates Produced by In Vitro Digestion Using Pepsin and Pancreatin by Santiago Gallegos Tintoré, Cristina Torres Fuentes, Javier Solorza Feria, Manuel Alaiz, Julio Girón Calle, Alma Leticia Martínez Ayala, Luis Chel Guerrero, Javier Vioque

“…The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse phase chromatography. …”
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Altered Phenotype of Circulating Dendritic Cells and Regulatory T Cells from Patients with Acute Myocarditis by Paola del Carmen Guerra-de-Blas, Daniela Cruz-González, Elena B. Martínez-Shio, Roberto González-Amaro, Héctor González-Pacheco, Esther Layseca-Espinosa, Carlos D. Escobedo-Uribe, Adriana E. Monsiváis-Urenda

“…Clinically, the increased levels of mDCs and their higher expression of costimulatory molecules correlate with a worse myocardial function, higher levels of acute phase reactants, and higher cardiac enzymes. This study shows an activating phenotype of circulating DCs from myocarditis patients. …”
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Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability by Guilong Wang, Zimin Liu, Li Xu, Yunjun Yan

“…The activities of mutant enzymes dropped to some extent towards p-nitrophenyl palmitate (pNPC16) and their optimum temperature was 35°C, which was 5°C lower than that of the wild-type. …”
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Alternative treatment methods for bovine mastitis: prospects and limitations (review) by V. D. Zubareva, O. V. Sokolova, M. V. Bytov, A. S. Krivonogova, S. V. Volskaya

“…This review describes the following alternative remedies: probiotics, bacteriocins, bacteriophages, phage enzymes (endolysins), nanoparticles, plant extracts, essential oils and immunobiological agents (vaccines). …”
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Mild Adrenal Steroidogenic Defects and ACTH-Dependent Aldosterone Secretion in High Blood Pressure: Preliminary Evidence by João Martin Martins, Sónia do Vale, Ana Filipa Martins

“…Mild defects of the 21-hydroxylase (21OHD) and 11-hydroxylase (11OHD) enzymes were common (42%). ACTH-dependent aldosterone secretion was found in most patients (54%) and characteristically in those with mild defects of adrenal steroidogenesis (>60%), PA (>75%), and otherwise in patients with apparent essential HBP (EHBP) (32%). …”
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Screening of α-glucosidase inhibitors in large-leaf yellow tea by offline bioassay coupled with liquid chromatography tandem mass spectrometry by You Wu, Zisheng Han, Mingchun Wen, Chi-Tang Ho, Zongde Jiang, Yijun Wang, Na Xu, Zhongwen Xie, Jinsong Zhang, Liang Zhang, Xiaochun Wan

“…Fluorescence-quenching analysis and protein-binding test revealed that the compounds of ethyl acetate fraction could inhibit α-glucosidase and α-amylase activities through binding to enzymes or other mechanisms. All chromatographic peaks of high-performance liquid chromatography (HPLC) of ethyl acetate fraction were separated and collected. …”
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