Protocol to identify endogenous proximal proteins using biotinylation by antibody recognition by Camilla Rega, Mercedes Pardo, Lesley-Ann Martin, Jyoti Choudhary

“…This approach differs from standard proximity labeling techniques, which rely on genetically engineered enzymes fused to the target protein.For complete details on the use and execution of this protocol, please refer to Rega et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Arachidonic Acid Derivatives and Their Role in Peripheral Nerve Degeneration and Regeneration by Carlos Rodrigo Camara-Lemarroy, Emmanuel Irineo Gonzalez-Moreno, Francisco Javier Guzman-de la Garza, Nancy Esthela Fernandez-Garza

“…Part of this neuroimmune signaling is mediated by the innate immune system, including arachidonic acid (AA) derivatives such as prostaglandins and leukotrienes. The enzymes responsible for their production, cyclooxygenases and lipooxygenases, also participate in nerve degeneration and regeneration. …”
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Alteration of Energy Substrates and ROS Production in Diabetic Cardiomyopathy by O. Lorenzo, E. Ramírez, B. Picatoste, J. Egido, J. Tuñón

“…Despite excess of plasma glucose and lipids, the diabetic heart almost exclusively depends on fatty acid degradation. Glycolytic enzymes and transporters are impaired by fatty acid metabolism, leading to accumulation of glucose derivatives. …”
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Research advances on encapsulation of probiotics with nanomaterials and their repair mechanisms on intestinal barriers by Xiaochen Wang, Mengxi Yu, Jianming Ye, Ting Liu, Lijuan Jian, Xiaoyan Zheng, Yuan Wang, Wei Song, Yane Luo, Tianli Yue

“…However, their viability and bioefficacy are adversely affected by gastrointestinal harsh conditions, such as gastric acid, bile salts and various enzymes. Fortunately, encapsulation based on various nanomaterials shows tremendous potential to protect probiotics. …”
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Lysyl oxidase inhibitors in colorectal cancer progression by Muxian Liu, Jie Wang, Meihong Liu

“…In this paper, we review the role of LOX enzymes in the development and progression of CRC. …”
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Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells by Max Nyström, Magnus Bergenfeldt, Irena Ljungcrantz, Èsa Lindeheim, Kjell Ohlsson

“…Secretory leucocyte protease inhibitor (SLPI) is a potent inhibitor of granulocyte elastase and cathepsin G, and also an inhibitor of pancreatic enzymes like trypsin, chymotrypsin and pancreatic elastase. …”
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The Use of Coupled Plasma Filtration Adsorption in Traumatic Rhabdomyolysis by Mario Pezzi, Silvia Renda, Anna Maria Giglio, Anna Maria Scozzafava, Simona Paola Tiburzi, Patrizia Casella, Fabrizio Iannelli, Mario Verre

“…Severe musculoskeletal injuries induce the release of sarcoplasmic elements such as muscle enzymes, potassium, and myoglobin in the systemic circulation. …”
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Absorption and Metabolism Characteristics of Rutin in Caco-2 Cells by Xiaofang Zhang, Jinhui Song, Xiaopeng Shi, Shan Miao, Yan Li, Aidong Wen

“…These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process.…”
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The structure–activity relationship and computational studies of 1,3-thiazole derivatives as cholinesterase inhibitors with anti-inflammatory activity by Modrić, Marina, Božičević, Marin, Odak, Ilijana, Talić, Stanislava, Barić, Danijela, Mlakić, Milena, Raspudić, Anamarija, Škorić, Irena

“…According to these experimental results, the cholinesterase inhibitory and anti-inflammatory activity of 1,3-thiazoles were correlated and confirmed that the same compounds inhibited LPS-stimulated TNF$\alpha $ cytokine production in PBMCs and enzymes cholinesterases.…”
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Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. by Daniel Manurung Josua, Uju, Santoso Joko, Sudiyani Yanni, Maryana Roni, Chasanah Ekowati

“…After pretreatment, the enzymatic hydrolysis process was carried out using cellulase enzymes on a shaker incubator at a temperature of 50oC, 150 rpm for 72 hours, then the fermentation process for 72 hours using Saccharomices cerevisiae mold. …”
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Surface Modification Chemistries of Materials Used in Diagnostic Platforms with Biomolecules by Mukesh Digambar Sonawane, Satish Balasaheb Nimse

“…Biomolecules including DNA, protein, and enzymes are of prime importance in biomedical field. …”
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Combined metabolomic and transcriptomic analysis to reveal the response of rice to Mn toxicity stress by Feng Li, Yushuang Yao, Jiapeng Ma, Zhengwei Wu, Dianfeng Zheng, Yingbin Xue, Ying Liu

“…Metabolic substances and genes associated with metabolic enzymes were identified. These results enhance our understanding of the molecular processes underlying the responses of rice to Mn toxicity stress and provide a basis for breeding Mn-tolerant rice varieties.…”
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Severe Hepatotoxicity in Mushroom Poisoning by Lepiota brunneoincarnata from Complete Recovery to Liver Transplantation: A Case Series with Review on Liver Function Tests and Liver... by Mohammad Hossein Anbardar, Neda Soleimani, Kourosh Kazemi, Zahra Jafarpour, Mahsa Hasani, Sahand Mohammadzadeh, Parnia Torfehnezhad, Sedighe Jafarian, Mahsa Farhadi, Mina Salari Sardari

“…Other patients followed a recovery process with a considerable drop in liver enzymes, especially ALT, during hospitalization utilizing conservative treatment. …”
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Effect of Dry Aging on Beef Muscle Proteins by Galina V. Gurinovich, Irina S. Patrakova, Vladislav A. Khrenov, Marina V. Patshina, Antonina I. Shevchenko

“…Long-time dry aging triggered proteolysis under the action of endogenous enzymes. The electropherogram analysis showed that the proteolytic changes in high-molecular myofibrillar proteins of high-quality beef became more pronounced after a longer maturation period. …”
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Therapeutic Values of Earthworm Species Extract from Azad Kashmir as Anticoagulant, Antibacterial, and Antioxidant Agents by Rozina Ghulam Mustafa, Andleeb Dr Saiqa, Jorge Domínguez, Madiha Jamil, Saira Manzoor, Samna Wazir, Bushra Shaheen, Asma Parveen, Rida Khan, Shaukat Ali, Nazish Mazhar Ali, Fatima Jalal, Sadaf Azad Raja

“…The coelomic fluid of earthworms possessed several bioactive compounds/enzymes/antioxidants that play an important role in the bacterial inhibition and act as anticoagulant agents. …”
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Analyzing cellulolytic bacteria diversity in mangrove ecosystem soil using 16 svedberg ribosomal ribonucleic acid gene by I. Dewiyanti, D. Darmawi, Z.A. Muchlisin, T.Z. Helmi

“…This bacteria could produce active enzymes needed to improve environmental quality by accelerating the organic matter decomposition. …”
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Maize Herbivore-Induced Volatiles Enhance Xenobiotic Detoxification in Larvae of <i>Spodoptera frugiperda</i> and <i>S. litura</i> by Peng Wang, Qiyue Zeng, Yi Zhao, Xiaomin Sun, Yongqiang Han, Rensen Zeng, Yuanyuan Song, Dongmei Chen, Yibin Lin

“…Furthermore, larval exposure to either maize HIPVs or DIMBOA induced the activities of cytochrome P450 enzymes (P450s), glutathione-s-transferase (GST), and carboxylesterase (CarE) in the midguts and fat bodies of the two insects, while the induction was significantly higher by the two components together. …”
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Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinen... by Gang Jiang, Fengyuan Yan, Yuanfeng Xu, Jianlin Li, Wenrong Feng, Guo an Hua, Wen jing Li, Jun Zhou, Yongkai Tang

“…Results demonstrated significantly elevated levels of antioxidant enzymes (T-AOC, SOD, and CAT), and immune enzymes (AKP, ACP) in treatments Th, Tv, and Thv, with a contrasting decrease in MDA contents. …”
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Host factor PLAC8 is required for pancreas infection by SARS-CoV-2 by Lesly Ibargüen-González, Sandra Heller, Darío López-García, Hanna Dietenberger, Thomas FE Barth, Patricia Gallego, Israel Fernández-Cadenas, Sayoa Alzate-Piñol, Catalina Crespí, Julieth A. Mena-Guerrero, Eugenia Cisneros-Barroso, Alejandro P. Ugalde, Gabriel Bretones, Charlotte Steenblock, Alexander Kleger, Marta L. DeDiego, Carles Barceló

“…Results We find that analysis of circulating pancreatic enzymes aid the stratification of patients according to COVID-19 severity and predicts outcomes. …”
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Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…However, treatment with H2S resulted in an increase in the expression of endogenous H2S enzymes and a decrease in pyroptosis-associated proteins compared to the Model group (p < 0.05). …”
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