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3581
Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements
Published 2025-01-01“…The anticoagulation assay showed that the four P2′ single-point mutants still had apparent inhibitory anticoagulation activity that selectively inhibited the human intrinsic coagulation pathway and had no influence on the extrinsic coagulation pathway or common coagulation pathway, which indicated that the single-point mutants had minimal effects on the anticoagulation activity of BF9-N17K. Interestingly, the enzyme inhibitor assay experiments showed that the XIa and plasmin inhibitory activities were significantly changed by the P2′ amino acid replacements. …”
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3582
gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16
Published 2025-01-01“…We found that gga-miR-6634-5p significantly down-regulated the mRNA levels of proliferation-related genes (CCND1, CDK1, and CDK6), decreased cell viability, the number of EdU-labelled positive cells, and the percentage of S-phase cells, as analysed by quantitative real time PCR (qRT-PCR), cell counting kit-8 (CCK-8), 5-ethynyl-2′deoxyuridine (EdU) and flow cytometry analyses (P < 0.01 or P < 0.05). qRT-PCR and enzyme-linked immunosorbent assay (ELISA) results demonstrated that gga-miR-6634-5p up-regulated the expression of steroid synthesis-related genes (CYP19A1, 3β-HSD, StAR and FSHR) (P < 0.01 or P < 0.05), as well as the secretion of estradiol (E2) and progesterone (P4) (P < 0.01 or P < 0.05). …”
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3583
Metabolic reprogramming of macrophages by PKM2 promotes IL-10 production via adenosine
Published 2025-01-01“…The tetramerization of the glycolytic enzyme pyruvate kinase M2 (PKM2) induces the production of the anti-inflammatory cytokine interleukin (IL)-10 in vivo, but the underlying mechanism remains elusive. …”
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3584
A Series of Novel Alleles of <i>Ehd2</i> Modulating Heading and Salt Tolerance in Rice
Published 2025-01-01“…<i>Ehd2</i> was revealed as a modulator of salt tolerance, likely through the regulation of ion transport, enzyme activity, and antioxidant systems. This study establishes <i>Ehd2</i> as a pivotal factor in promoting heading while negatively regulating salt tolerance in rice.…”
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3585
Biosynthesis inhibition of miR-142-5p in a N 6-methyladenosine-dependent manner induces neuropathic pain through CDK5/TRPV1 signaling
Published 2025-01-01“…In addition, an in vivo rat chronic constriction injury (CCI) model was established, and miR-142-5p agomir or antagomir was injected intrathecally. An enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of IL-6 and TNF. …”
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3586
A phase 1b clinical trial to determine the safety, tolerability and immunogenicity of simian adenovirus and poxvirus vectored vaccines against Mycobacterium avium complex subspecie...
Published 2025-03-01“…Secondary objectives of immunogenicity were assessed by ELISpot (enzyme-linked immunosorbent spot) and clinical response by Crohn’s Disease Activity Index (CDAI) and Simple Endoscopic Score for Crohn’s Disease (SES-CD). …”
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3587
Decreasing of heterogeneity in black tiger prawn (Penaeus monodon) and white shrimp (Litopenaeus vannamei) broodstocks and their fry produced in hatchery
Published 2009-01-01“…Amplification of black tiger shrimp mtDNA used primers designed based on 16S rDNA sequences, and the products were restricted by Nde II enzyme. While the primers for white shrimp was based on COIL and COIH sequences, and their PCR products were digested with Nla III. …”
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3588
Characterization of the Phosphotransferase from <i>Bacillus subtilis</i> 1101 That Is Responsible for the Biotransformation of Zearalenone
Published 2025-01-01“…This study contributes to the enzymatic removal of ZEA and broadens the spectrum of strain and enzyme options available to researchers for ZEA detoxification efforts.…”
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3589
Effect of the Iridoid-rich Fraction from <italic>Valeriana jatamansi</italic> Jones on Oxidative Stress in Rats with Spinal Cord Injury
Published 2019-06-01“…The results of the biochemical kit tests showed that the activity of GSH-PX and CAT antioxidant enzymes in the spinal cord of the model group was lower than that in the sham-operated group; compared with the model group, in the treatment group with iridoid-rich fraction from <italic>Valeriana jatamansi</italic> Jones the activity of the antioxidant enzymes of GSH-PX(<italic>P</italic>< 0.05)and CAT(<italic>P</italic>< 0.01)of the spinal cord tissue increased significantly. …”
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3590
PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp.
Published 2003-05-01“…Development of digestive enzymes; protease, lipase and amylase were observed in patin catfish, Pangasius hypophthalmus, larvae. …”
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3591
Transketolase attenuates the chemotherapy sensitivity of glioma cells by modulating R-loop formation
Published 2025-01-01“…Here, we show that transketolase (TKT), a metabolic enzyme in the pentose phosphate pathway (PPP), attenuates the chemotherapy sensitivity of glioma cells in a manner independent of catalytic activity. …”
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3592
Effect of Xingbi Gel Nasal Drops on Rats with Allergic Rhinitis Based on the Nasal Mucosa-Hippocampus Neuroimmune Mechanism
Published 2024-08-01“…The AR rat behavioral scoring criteria was used to evaluate rhinitis behavior of rats; Morris water maze test was used to evaluate learning and memory function of rats; enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of immunoglobulin E (IgE) and transforming growth factor-β1 (TGF-β1); hematoxylin-eosin (HE) staining was used to observe pathological changes of nasal mucosa and eosinophil (EOS) count; immunohistochemistry method was used to detect expression levels of non-receptor tyrosine kinases Fyn, substance P (SP), vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the hippocampus; and real-time polymerase chain reaction (RT-PCR) was used to detect mRNA transcription levels of Fyn, SP, VIP and NPY in the hippocampus.Results(1) Behavioral scores of rhinitis: compared with the normal group, behavioral scores of rhinitis were significantly higher in the model group, the traditional Chinese medicine group and the western medicine group before treatment (<italic>P</italic><0.05). …”
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3593
ENO1 promotes PDAC progression by inhibiting CD8+ T cell infiltration through upregulating PD-L1 expression via HIF-1α signaling
Published 2025-02-01“…Under hypoxic conditions, the “Warburg effect” occurs to meet the nutrient and energy demands of rapid genome replication, remodeling the tumor microenvironment (TME) and influencing tumor immunity. α-Enolase (ENO1) is a multifunctional protein, acting as a glycolytic enzyme that catalyzes the conversion of 2-phosphoglyceric acid to phosphoenolpyruvic acid. …”
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3594
Hesperidin enhances broiler growth performance by augmenting gastric acid secretion via the proton pump pathway
Published 2025-02-01“…Proventriculus and gizzard tissues were used to evaluate pepsinogen levels, perform hematoxylin-eosin (H&E) staining, conduct enzyme-linked immunosorbent assays (ELISAs) for key proton pump components, and assess proton pump activity. …”
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3595
Epithelial NSD2 maintains FMO‐mediated taurine biosynthesis to prevent intestinal barrier disruption
Published 2024-12-01“…Mechanistically, NSD2 loss led to downregulation of H3K36me2 and flavin‐containing monooxygenase (FMO) (taurine‐synthesis enzyme) mRNA, resulting in decreased taurine biosynthesis in IECs. …”
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3596
Effect of Simultaneous Use of Different Amounts of Biochar and Soil Application of Common Sulfur and Spraying of Nanosulfur on Yield and Yield Components and Some Phytochemical Cha...
Published 2025-09-01“…Sulfur is a part of an enzyme necessary for nitrogen absorption. Its deficiency can significantly hinder nitrogen metabolism, as sulfur and nitrogen are required to form amino acids essential for protein synthesis. …”
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3597
SLC31A1 promotes chemoresistance through inducing CPT1A-mediated fatty acid oxidation in ER-positive breast cancer
Published 2025-03-01“…Mechanistically, we observed that in chemoresistant breast cancer cell lines, SLC31A1 knockdown resulted in a substantial decrease in the expression of carnitine palmitoyltransferase 1A (CPT1A), a rate-limiting enzyme of fatty acid oxidation (FAO). RNA-Seq analysis indicated that FAO might be implicated in SLC31A1-mediated breast cancer progression. …”
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3598
Microbiology /
Published 2005Table of Contents: “…The history and scope of microbiology -- The study of microbial structure: microscopy and specimen preparation -- Procaryotic cell structure and function -- Eucaryotic cell structure and function -- Microbial nutrition -- Microbial growth -- Control of microorganisms by physical and chemical agents -- Metabolism: energy, enzymes, and regulation -- Metabolism: energy release and conservation -- Metabolism: the use of energy in biosynthesis -- Genes: structure, replication, and mutation -- Genes: expression and regulation -- Microbial recombination and plasmids -- Recombinant DNA technology -- Microbial Genomics -- The viruses: introduction and general characteristics -- The viruses: bacteriophages -- The viruses: viruses of eucaryotes -- Microbial taxonomy -- The archaea -- Bacteria: the deinococci and nonproteobacteria gram negatives -- Bacteria: the proteobacteria -- Bacteria: the Low G 1 C gram positives -- Bacteria: the High G 1 C gram positivies -- The fungi (eumycota), slime molds, and water molds -- The algae -- The protozoa -- Microorganism interactions and microbial ecology -- Microorganisms in aquatic environments -- Microorganisms in terrestrial environments -- Normal microbiota and nonspecific host resistance -- Specific immunity -- Medical immunology -- Pathogenicity of microorganisms -- Antimicrobial chemotherapy -- Clinical microbiology -- The epidemiology of infectious disease -- Human diseases caused by viruses -- Human diseases caused by bacteria -- Human diseases causedd by fungi and protozoa -- Microbiology of food -- Industrial microbiology and biotechnology.…”
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3599
A humanized anti-MSLN×4-1BB bispecific antibody exhibits potent antitumour activity through 4-1BB signaling activation and fc function without systemic toxicity
Published 2025-01-01“…Methods A novel anti-MSLN×4-1BB bispecific antibody (bsAb) was generated via antibody engineering, and its affinity and activity were detected via enzyme-linked immunosorbent assay (ELISA), flow cytometry, and T-cell activation and luciferase reporter assays. …”
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3600
Beyond Dermatological Findings: Multisystem Involvement in Prolidase Deficiency
Published 2025-01-01“…Metabolic evaluations revealed low prolidase enzyme activity in P1 and P2. Conclusion: Beyond the well-known classical dermatological findings, the presence of recurrent opportunistic infections, gastrointestinal involvement, malignancy, and flow cytometry findings suggestive of combined immunodeficiency indicate that the diagnosis of prolidase deficiency may be underestimated. …”
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