Structural and functional features of phytoene synthase isoforms PSY1 and PSY2 in pepper Capsicum annuum L. cultivars by E. A. Dyachenko, M. A. Filyushin, G. I. Efremov, E. A. Dzhos, A. V. Shchennikova, E. Z. Kochieva

“…The Capsicum annuum genome contains two isoforms of this enzyme, localized in leaf (PSY2) and fruit (PSY1) plastids. …”
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Exploring the Analgesic Effect of Acupuncture on Knee Osteoarthritis Based on MLT/cAMP/PKA/CREB Signaling Pathway by Zhang C, Yu M, Zhang L, Zhou X, Han J, Fu B, Xue H, Zhang C

“…Meanwhile, MLT in serum, cyclic adenosine monophosphate (cAMP) in cartilage, and matrix metalloproteinase-3 (MMP-3) in joint fluid were detected by enzyme-linked immunosorbent assay. In addition, the expression of aromatic L-amino acid N-acetyltransferase (AANAT), melatonin receptor 1 (MT1) and 2 (MT2) mRNAs in cartilage was determined by real-time quantitative reverse transcription-polymerase chain reaction, and the levels of proteins related to PKA/CREB signaling pathway were detected by Western blotting.Results: Based on the results of Lequesne MG score and Pain assessment by hot plate test experimental data, the treatment group presented significant improvements in knee pain and overall function relative to OA (Osteoarthritis) group. …”
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Identification and Analysis of Melon (<i>Cucumis melo</i> L.) SHMT Gene Family Members and Their Functional Studies on Tolerance to Low-Temperature Stress by Yanmin Liu, Dandan He, Yizhou Wu, Kangqi Zhao, Changyi Yang, Yulu Zhong, Liuyang Yang, Haiyue Niu, Sushuang Liu

“…Serine hydroxymethyltransferase (SHMT), a conserved phosphopyridoxal-dependent enzyme, plays a crucial role in plant resistance to abiotic stressors. …”
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Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China by Yuxin Li, Yinan Wang, Xin Gao, Lihong Luo, Bohan Zhang, Xiao Wang, Jing Li, Ruijia Wu, Lixin He, Wenxuan Li, Changwei Qiu

“…The expression of two aminoglycoside modifying enzyme genes, <i>aacA-aphD</i> and <i>aac (6′)-Ib</i>, and three 16S rRNA methyltransferase genes, <i>rmtB</i>, <i>rmtE</i> and <i>npmA</i>, were analyzed by PCR. …”
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Telomerase inhibiting phytochemicals derived from Blumea eriantha for cancer treatment: A comprehensive computational analysis by Rahil Ummar Faruk Abbu, Shaik Mohammad Abdul Fayaz, Divyashree M S, Raghu Chandrashekhar H, Prasanna Kumar Reddy Gayam, Archana Mahadev Rao

“…Background: One potential treatment target for cancer cells is telomerase, an essential enzyme involved in the longevity of cancer cells. …”
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Postoperative Tongqi Formula ameliorates postoperative ileus via p38 MAPK signaling pathway and metabolic disorder by Mengmeng Yang, Yuxuan Lu, Shufan Jin, Wanqiu Liu, Miaoshi Yao, Zhiwei Jiang, Yachun Shu

“…Inflammatory cytokine levels were quantified using enzyme-linked immunosorbent assay (ELISA). Subsequently, Western blot analysis was performed to examine the p38 MAPK signaling pathway. …”
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Efficacy and safety of tripterygium glycosides combined with ACEI/ARB on diabetic nephropathy: a meta-analysis by Zhuan’E. Yao, Zhuan’E. Yao, Pengbo Wang, Qinjuan Fu, Qiong Song, Haojian Xu, Peng Zhang

“…AimsThis study aims to evaluate the efficacy and safety of tripterygium glycosides combined with angiotensin-converting enzyme inhibitors/angiotensin receptor blockers (ACEI/ARBs) in treating Diabetic nephropathy and provide high-level evidence to support its standardized application.MethodsLiteratures were retrieved from PubMed, Web of Science, EMBASE, Cochrane Library, CNKI, Wanfang and VIP databases, the search time frame was defined as from the time of establishment to April 2023. …”
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Development of <i>Aspergillus oryzae</i> BCC7051 as a Robust Cell Factory Towards the Transcriptional Regulation of Protease-Encoding Genes for Industrial Applications by Sarocha Panchanawaporn, Chanikul Chutrakul, Sukanya Jeennor, Jutamas Anantayanon, Kobkul Laoteng

“…Enzyme-mediated protein degradation is a major concern in industrial fungal strain improvement, making low-proteolytic strains preferable for enhanced protein production. …”
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Association between ABCB4 variants and intrahepatic cholestasis of pregnancy by Dekun Zhang, Yuhong Li, Shufeng He, Jing Shu, Tiechen Li, Qing Sun

“…Abstract The ABCB4 gene encodes multidrug resistance protein 3(MDR3), which is a phosphatidylcholine(PC) transfer enzyme that transfers lecithin from the inner part of the phospholipid bilayer to the extracellular bile. …”
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Aurora kinase as a putative target to tick control by Bruno Moraes, Helga Gomes, Luiz Saramago, Valdir Braz, Luís Fernando Parizi, Gloria Braz, Itabajara da Silva Vaz, Carlos Logullo, Jorge Moraes, Ala Tabor

“…The characterization of exclusive regions of the enzyme will enable new studies aimed at promoting species-specific enzymatic inhibition in ectoparasites.…”
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Excessive copper induces lignin biosynthesis in the leaves and roots of two citrus species: Physiological, metabolomic and anatomical aspects by Xin Zhou, Xing Xiong, Fei Lu, Wenqing Shi, Yu Zhou, Ningwei Lai, Li-Song Chen, Zeng-Rong Huang

“…The histochemical staining supported that Cu stress improved the deposition of both guaiacy and syringy lignin units in citrus roots. The enzyme activity and gene expression revealed that activating lignin-biosynthetic enzymes, such as L-phenylalanine ammonia-lyase, peroxidase and laccase, played an essential role in lignin biosynthesis. …”
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Effects of supplementation with vitamin D3 on growth performance, lipid metabolism and cecal microbiota in broiler chickens by Jiawei Li, Ximei Li, Jiamin Tian, Linna Xu, Yan Chen, Susu Jiang, Guohua Zhang, Jianxiong Lu

“…VD3 increased mRNA expressions of genes related to de novo lipogenesis (DNL) (mTOR, SREBP-1c, FAS and ACC), lipid oxidation (AMPK, PPARα, CPT-1α and ACO) and lipid transport (ApoB and MTTP), and FAS, ACC and CPT1 enzyme activities in the liver. However, mRNA levels of genes involved in DNL and cellular lipid uptake (LPL and FATP1) and LPL activity were decreased in abdominal adipose tissue, and that of genes involved in lipid oxidation and lipolysis (HSL and ATGL) was increased by VD3. …”
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Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health by Raden Ayu Tanzila, Irfannuddin Irfannuddin, Yudianita Kesuma, Iche Andriyani Liberty, Miranti Dwi Hartanti, Ni Made Elva Mayasari, Wieke Anggraini, Liza Chairani, Thia Prameswarie, Indri Ramayanti, Wulan Aristi, Delvi Larasari

“…Standard laboratory protocols for serum lipid measurements, deoxyribonucleic acid (DNA) extraction, allele analysis by polymerase chain reaction (PCR), and LEP and insulin analysis by enzyme-linked immunosorbent assay (ELISA) were employed. …”
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Antigenicity, Solubility and Digestibility of β-Lactoglobulin Combined with Different Plant Polyphenols by Liyan NONG, Xueming LIU, Jingrong CHENG, Jinhao ZOU, Wenhong CAO, Daobang TANG, Huaigu YANG

“…By means of the determination of allergenicity in vitro (through enzyme linked immunosorbent assay and immunoblotting), the six kind of plant polyphenols extracts could reduce the antigenicity (specific IgG binding capacity) of β-lactoglobulin to different extents (under alkaline and enzymatic treatment), in which longan pericarp and seed polyphenols exhibited the best (the mean of antigenic inhibition rate: 76.8%, 77.2%, respectively). …”
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Influence of Titanium Alloy Scaffolds on Enzymatic Defense against Oxidative Stress and Bone Marrow Cell Differentiation by Lais Morandini Rodrigues, Elis Andrade Lima Zutin, Elisa Mattias Sartori, Daniela Baccelli Silveira Mendonça, Gustavo Mendonça, Yasmin Rodarte Carvalho, Luana Marotta Reis de Vasconcellos

“…After 24 hours, cells plated on the scaffolds were analyzed by scanning electron microscopy (SEM). The antioxidant enzyme activity was measured 72 hours after cell plating. …”
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Lower Plasma β-Amyloid 1-42 Levels in Amnestic Mild Cognitive Impairment Compared to Healthy Individuals by Cynthia Cynthia, Jusak Nugraha, Muhammad Hamdan, Hari Basuki Notobroto, Silvia Francina Lumempouw, Rahajuningsih Dharma

“…The plasma levels of β-Amyloid 1-42 were measured using an enzyme-linked immunosorbent assay (ELISA) methods, while the ApoE ε4 allele genotyping was conducted using polymerase chain reaction (PCR) techniques. …”
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Effects of Omega-3 Fatty Acid in Nonalcoholic Fatty Liver Disease: A Meta-Analysis by Wenxia Lu, Sainan Li, Jingjing Li, Jianrong Wang, Rong Zhang, Yuqing Zhou, Qin Yin, Yuanyuan Zheng, Fan Wang, Yujing Xia, Kan Chen, Tong Liu, Jie Lu, Yingqun Zhou, Chuanyong Guo

“…A meta-analysis was conducted to assess the effect of omega-3 fatty acid supplementation (n-3 PUFAs) in lowering liver fat, liver enzyme (alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyltransferase (GGT) levels), and blood lipids (triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), and low density lipoprotein (LDL)) in patients with nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH). …”
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Neutrophil extracellular traps (NETs) are increased in rheumatoid arthritis-associated interstitial lung disease by Jing Xue, Miaomiao Nian, Yangyang Liang, Zeqin Zhu, Zhenyu Hu, Yuanyuan Jia, Shuhong Chi, Juan Chen

“…The clinical relevance of plasma myeloperoxidase-DNA (MPO-DNA), citrullinated histone 3 (Cit-H3), and cell-free DNA was evaluated in RA-ILD patients with different imaging types via a commercial enzyme-linked immunosorbent assay (ELISA). Results In the ZYM-treated SKG mouse model, which recapitulates key features of RA-ILD, an increased population of neutrophils in the lung tissue was primarily responsible for NET formation. …”
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Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice by Guangshan Zhao, Jianyuan Teng, Ruixia Dong, Qiuyan Ban, Lian Yang, Kang Du, Yifei Wang, Hanlin Pu, Chung S. Yang, Zhe Ren

“…The underlying mechanisms involve downregulation of gluconeogenesis (lower protein levels of TXNIP and FBP and enzyme activity of FBP), upregulation of lipid catabolism (higher protein levels of CPT-1α and PPARα), downregulation of lipogenesis (lower protein level of SREBP-1), and modification of the structure and abundance of gut microbiota to modulate metabolic homeostasis. …”
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The value of inflammation-related indicators in chemotherapy efficacy and disease-free survival of triple-negative breast cancer by Jie Zhu, Jiale Cheng, Yuyuan Ma, Ying Wang, Zhonghua Zou, Wenjie Wang, Haihua Shi, You Meng

“…Levels of interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assays (ELISAs). Platelet-to-lymphocyte ratio (PLR) and immune inflammatory index (SII) were obtained from blood routine. …”
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