Growth performance, intestinal health, and non-specific immunity were significantly affected by feeding different compound lactic acid bacteria supplementation in sea urchin (Stron... by Yuntian Zhang, Yuntian Zhang, Rongwei Zhang, Rongwei Zhang, Yi Chen, Yi Chen, Zhixu Guo, Zhixu Guo, Xiangyu Meng, Xiangyu Meng, Yuzhe Han, Yuzhe Han, Xiaoran Zhao, Xiaoran Zhao, Tongjun Ren, Tongjun Ren

“…Notably, compound lactic acid bacteria improved digestive enzyme activities in the intestine and reduced the feed conversion ratio (FCR). …”
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A novel platform for engineered AAV-based vaccines by Sabrina Babutzka, Miranda Gehrke, Anastasia Papadopoulou, Maria Diedrichs-Möhring, Maria Giannaki, Lena Hennis, Bastian Föhr, Cale Kooyman, Andreas Osterman, Evangelia Yannaki, Gerhild Wildner, Hermann Ammer, Stylianos Michalakis

“…This engineering endowed AAV.RBDs with SARS-CoV-2-like properties, such as angiotensin-converting enzyme 2 receptor affinity. In line with this, AAV.RBDs were neutralized by sera from human donors vaccinated against SARS-CoV-2. …”
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Comprehensive metabolite profiling and evaluation of anti-nociceptive and anti-inflammatory potencies of Nypa fruticans (Wurmb.) leaves: Experimental and in-silico approaches by Farhana Islam, Md. Aktaruzzaman, Md. Tarikul Islam, Fariya Islam Rodru, Saquiba Yesmine

“…The identified compounds were subsequently evaluated by computational studies against the cyclooxygenase-2 enzyme. EENF demonstrated significant antioxidant activity in both the DPPH scavenging assay (IC50: 105.18 μg/mL) and the reducing power assay (RC50:1752.76 μg/mL). …”
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Molecular Characterization, Oxidative Stress-Mediated Genotoxicity, and Hemato-Biochemical Changes in Domestic Water Buffaloes Naturally Infected with <i>Trypanosoma evansi</i> Und... by Waqas Ahmad, Muhammad Yasin Tipu, Muti ur Rehman Khan, Haroon Akbar, Aftab Ahmad Anjum, Muhammad Ovais Omer

“…Alongside significant (<i>p</i> < 0.05) reductions in red cell indices, a marked elevation in leukocyte counts and serum hepatic enzyme levels was recorded in the affected buffaloes. (4) Conclusion: <i>T. evansi</i> isolates of buffaloes from Multan, Pakistan, have genetic similarities to Indian isolates. …”
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Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening by Wei Pu, Jinhui Feng, Jiuzhou Chen, Jiao Liu, Xuan Guo, Lixian Wang, Xiaojia Zhao, Ningyun Cai, Wenjuan Zhou, Yu Wang, Ping Zheng, Jibin Sun

“…Subsequently, the SerRF104I-based biosensor was employed for high-throughput screening of the superior enzyme mutants of l-homoserine dehydrogenase and γ-glutamyl kinase, which are critical enzymes in the biosynthesis of l-threonine and l-proline, respectively. …”
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Free Fatty Acids in CSF and Neurological Clinical Scores: Prognostic Value for Stroke Severity in ICU by Sayed Gaber, Sherine Ibrahim ElGazzar, Mahmoud Qenawi, Nora Ismail Mohamed Abbas

“…The FFA levels were measured using the sensitive enzyme-based colorimetric method. The NIHSS, GCS, and mRS were evaluated at admission and at 30 days. …”
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Inhibition Mechanism of Pleurotus citrinipileatus Sing. Ergothioneine on Pancreatic Lipase Activity by Zhangchen XIA, Xiaohui MENG, Ru FANG, Yanqing MAO, Junwen CHENG, Yanbin WANG, Juan XU, Liang HE

“…The interaction between Pleurotus citrinipileatus Sing. ergothioneine (PCEGT) and pancreatic lipase (PL) was studied by analyzing multiple spectroscopies including enzyme kinetics, ultraviolet absorption (UV), fluorescence spectroscopy, synchronous fluorescence spectroscopy and 3D fluorescence spectroscopy as well as the PCEGR-PL interaction mechanism by molecular docking in order to clarify its inhibitory mechanism. …”
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Evaluation of Inflammatory Cytokine Secretion by Human Alveolar Macrophages by J. E. Losa García, F. M. Rodríguez, M. R. Martín de Cabo, M. J. García Salgado, J. P. Losada, L. G. Villarón, A. J. López, J. L. P. Arellano

“…IL-1β, TNF-α, IL-6 and IL-8 levels were measured in BAL fluid and culture supernatant using specific enzyme-linked immunosorbent assays. The substance found to stimulate the secretion of inflammatory cytokines to the greatest extent was LPS at a concentration of 10 μg/ml. …”
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Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis by Liming Liu, Cuiqing Zhao, Ying Yang, Xiaoxia Kong, Tuo Shao, Li Ren, Xinyu Zhuang, Baishuang Yin, Gerald Dryden, Craig McClain, Weimin Luan, Wenke Feng

“…Lipolysis index and adipose lipolytic enzymes were determined. In addition, 3T3-L1 cells were pretreated with IL-6, followed by recombinant human FGF21 (rhFGF21) treatment; lipolysis was assessed. …”
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Comparative analysis of the primary structure and production of recombinant poly(ADP-ribose)polymerase 1 of long-lived Heterocephalus glaber by K. N. Naumenko, A. R. Nurislamov, K. D. Nazarov, V. S. Fishman, A. A. Popov, I. O. Petruseva, A. N. Evdokimov, O. I. Lavrik

“…The central regulator of the DNA repair process is the enzyme poly(ADP-ribose)polymerase 1 (PARP1). PARP1 catalyzes the synthesis of poly(ADPribose) polymer (PAR) upon DNA damage using nicotinamide adenine dinucleotide (NAD+) as a substrate. …”
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Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics by Huifang Li, Ni Deng, Jiayi Yang, Yang Zhao, Xiaoxuan Jin, Ang Cai, Navindra P. Seeram, Hang Ma, Dongli Li, Huilan Yang, Chang Liu, Chang Liu

“…This was supported by findings from several cellular assays showing that oleuropein reduced the level of IL-1β and inhibited the activity of caspase-1/IL-1 converting enzyme, as well as ameliorated pyroptosis in HaCaT cells. …”
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Antioxidant, Anti-Tyrosinase, and Wound-Healing Capacities of Soy Protein Hydrolysates Obtained by Hydrolysis with Papaya and Cantaloupe Juices Showing Proteolytic Activity by Thi-Phuong Nguyen, Quang Thai Le, Mai Linh Thi Tran, Kim Nhung Ta, Khoa Thi Nguyen

“…A higher DH (about 26%) was obtained by the hydrolysis with cantaloupe juice at the same juice to substrate ratio and treatment conditions. Papain used at the enzyme to substrate ratio of 0.625:2 ( w/w ) broke down SPI in a similar DH as papaya juice at the juice to substrate ratio of 2.5:2 ( v/w ). …”
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The Role of Mature Brain-Derived Neurotrophic Factor and Its Precursor in Predicting Early-Onset Insomnia in Stroke Patients Experiencing Early Neurological Deterioration by Shi G, Yu P, Wang Z, Xu M, Guo M, Wang X, Zhou R

“…Serum levels of mBDNF and proBDNF were quantified using enzyme-linked immunosorbent assays. EOI was diagnosed according to the International Classification of Sleep Disorders, Third Edition (ICSD-3). …”
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Evolocumab safety and efficacy in hypercholesteremia patients with or without diabetes: a retrospective real-world analysis by Sultan Ibrahim Alraddadi, Hind Almodaimegh, Abdullah Kharbosh, Hadeel Alharbi, Ahmed Ibrahim Fathelrahman, Mona Yaser Alsheikh, Lama Alfehaid

“…Efficacy was measured by LDL-C reduction and target achievement, while safety was assessed by examining glycemic control changes, new-onset diabetes (NOD) and hepatic enzyme levels. Data analysis included descriptive and comparative methods, with significance set at p < 0.05. …”
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Phytochemical and Biological Investigations of Crude Extracts of <i>Astragalus pisidicus</i> by Esra Aydemir, Elif Odabaş Köse, Serap Özkaya Gül, Alaaddin Korkut, A. Cansu Kilit, Mehmet Engin Celep, Mustafa Yavuz, R. Süleyman Göktürk, Cengiz Sarikurkcu

“…The elevation of caspase 2, 3, 6, 8, and 9 enzyme activities, along with DNA fragmentation, signifies that the mode of cell death is apoptosis. …”
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Inflammatory Cytokine Profile Associated with Metabolic Syndrome in Adult Patients with Type 1 Diabetes by Aldo Ferreira-Hermosillo, Mario Molina-Ayala, Claudia Ramírez-Rentería, Guadalupe Vargas, Baldomero Gonzalez, Armando Isibasi, Irma Archundia-Riveros, Victoria Mendoza

“…We determined serum IL-6, IL-8, IL-10, and TNF with flow cytometry and adiponectin and resistin concentrations with enzyme linked immunosorbent assay in patients with and without metabolic syndrome. …”
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Date pomace polysaccharides-capped selenium nanoparticles: Biosynthesis, optimization, physicochemical characterization, biological activities, stability and gut microbiota modulat... by Gafar Babatunde Bamigbade, Athira Subhash, Mohammed Abdin, Hebah Jarusheh, Basim Abu-Jdayil, Shao-Quan Liu, Giovanni Palmisano, Abdelmoneim Ali, Afaf Kamal Eldin, Mutamed Ayyash

“…They also showed concentration-dependent enzyme inhibition: 86.8 % α-amylase, 53.9 % α-glucosidase, and 42.4 % ACE inhibition at 100 mg/L, along with anticancer effects against Caco-2 (32.3 %) and MCF-7 (11.3 %) at 50 mg/L. …”
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Preparation and Characterisation of Polypyrrole-Iron Oxyhydroxide Nanocomposite as Sensing Material by Farah Nabila Diauudin, Siti Aminah Mohd Noor, Jahwarhar Izuan Abdul Rashid, Victor Feizal Knight, Wan Md Zin Wan Yunus, Keat Khim Ong, Noor Azilah Mohd Kasim, Safura Taufik, Alinda Samsuri, Intan Juliana Shamsudin, Wahhida Latip

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Cyclophilin–CD147 interaction enables SARS-CoV-2 infection of human monocytes and their activation via Toll-like receptors 7 and 8 by Gabor Tajti, Laura Gebetsberger, Gregor Pamlitschka, Katharina Aigner-Radakovics, Judith Leitner, Peter Steinberger, Hannes Stockinger, Anna Ohradanova-Repic

“…Firstly, SARS-CoV-2 infects monocytes in a process independent of the S protein and the prime SARS-CoV-2 receptor angiotensin-converting enzyme 2. Instead, the alternative SARS-CoV-2 receptor CD147, which is highly expressed on monocytes, recognizes its well-known interaction partners cyclophilins A and B that are incorporated into SARS-CoV-2 virions. …”
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Inhibition of p70 Ribosomal S6K1 Protects the Myocardium against Ischemia/Reperfusion-Induced Necrosis through Downregulation of RIP3 by Hui Shang, Jinjin Shi, Jun Zhu, Yunfeng Guo, Xiaoyan Wang

“…The expression levels of cardiac troponin-1 (cTn-1), lactate dehydrogenase (LDH), creatine kinase MB (CK-MB), and aspartate aminotransferase (AST) were determined by enzyme-linked immunosorbent assay (ELISA). Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining and propidium iodide (PI) staining were used to examine the apoptosis and necrosis of myocardial tissues. …”
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