Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 by Hanie Pooshideh, Jalal Jafarzade, Ali Heidarpour, Mojtaba Taghizade Armaki, saeid Mahdavi Omran, Firoozeh Kermani, Mohsen Karami

“…The PCR-RFLP method and the restriction enzymes Msp I and Bln I were used for the differentiation of Candida species, and the restriction enzyme Alw I for Aspergillus species. …”
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Immune response profiling of HERV-W envelope proteins in multiple sclerosis: potential biomarkers for disease progression by Stefano Ruberto, Stefano Ruberto, María I. Domınguez-Mozo, M. Angel Garcıa-Martınez, Davide Cossu, Davide Cossu, Leonardo A. Sechi, Leonardo A. Sechi, Roberto Alvarez-Lafuente

“…This study aims to evaluate both humoral and cell-mediated immune response to antigenic peptides derived from these proteins across different clinical forms and inflammatory phases of MS.MethodsIndirect enzyme-linked immunosorbent assay (ELISA) was employed to measure immunoglobulin G (IgG) responses to syncytin-1env 486-500 and pHERV-Wenv 486-504 peptides in MS patients. …”
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Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases in Escherichia coli by Ayesha Siddiqui, Muhammad Mudassar Iqbal, Asad Ali, Iqra Fatima, Hazrat Ali, Aamir Shehzad, Sameer H. Qari, Ghulam Raza, Muhammad Aamer Mehmood, Peter J. Nixon, Niaz Ahmad

“…Furthermore, the activity assays confirmed that the recombinant enzymes maintained their activity at elevated temperatures. …”
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Hepatotoxicity of statins: a real-world study based on the US Food and Drug Administration Adverse Event Reporting System database by Bojing Wang, Shu Huang, Shu Huang, Shiqi Li, Yaqi Deng, Ziyan Li, Yizhou Wang, Xiaomin Shi, Wei Zhang, Lei Shi, Xiaohong Wang, Xiaowei Tang

“…Fluvastatin also showed notable signal strength in autoimmune hepatitis, while simvastatin had a relatively weaker signal strength for hepatic enzyme increased.ConclusionThis study discovered specific adverse event signal values, revealing potential hepatotoxic risks associated with the use of statin drugs. …”
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Genetic Deletion and Pharmacological Inhibition of PI3Kγ Reduces Neutrophilic Airway Inflammation and Lung Damage in Mice with Cystic Fibrosis-Like Lung Disease by Maria Galluzzo, Elisa Ciraolo, Monica Lucattelli, Eriola Hoxha, Martina Ulrich, Carlo Cosimo Campa, Giuseppe Lungarella, Gerd Doring, Zhe Zhou-Suckow, Marcus Mall, Emilio Hirsch, Virginia De Rose

“…Treatment with the PI3Kγ inhibitor AS-605240 decreased the number of neutrophils in BALF of βENaC-Tg mice, reproducing the effect observed with genetic deletion of the enzyme. Conclusions. These results demonstrate the biological efficacy of both genetic deletion and pharmacological inhibition of PI3Kγ in reducing chronic neutrophilic inflammation in CF-like lung disease in vivo.…”
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Fisiologi dan Gangguan Keseimbangan Natrium, Kalium dan Klorida serta Pemeriksaan Laboratorium by Rismawati Yaswir, Ira Ferawati

“…Hipo- happened when the electrolyte concentration in body go down more than some<br />miliekuivalen under normal values and hyper- when the concentration of mounting above normal.Laboratory<br />findings to determine concentration of natrium, chloride and potassium are with ion selective electrode (ISE)<br />method, flame emission spectrophotometry (FES), atomic absorption spectrophotometry, spektrofotometry<br />pursuant to enzyme activation, determine concentration of chloride with titration method of merkurimeter, and with<br />titration method of colorimetry-amperometry.…”
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Dual Role of &alpha;-MSH in Colitis Progression: Mediating Neutrophil Differentiation via Bone Marrow by Liao X, Liu H, Li Y, Zhang W, Dai Q, Wei H, Zhou J, Xie X, Zhou H

“…This study aims to investigate the alterations in the HPA and its potential roles during IBD development.Methods: We utilized a dextran sodium sulfate (DSS)-induced colitis model in mice and employed immunofluorescence, real-time quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), among other techniques, to evaluate the impact of colitis on the HPA. …”
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The prognostic role of vasculoendothelial growth factor A in patients with ST-segment elevation acute myocardial infarction during 6-months follow-up period by N.P. Kopytsya, I.M. Кutya, I.V. Rodionova, Ya.V. Hilova

“…The level of VEGF-A was determined by enzyme immunoassay. Blood for determining VEGF-A was taken on the 5-7th day of the disease. …”
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Vitamin D3/VDR alleviates double-stranded RNA virus -induced biliary epithelial cell damage by inhibiting autophagy by Na Liu, Pu Zhao, Ping Cao, JunPeng Hui, YongKang Pan, Jiwen Cheng

“…The secretory levels of inflammatory factors were analyzed using enzyme-linked immunosorbent assay. A BA mouse model was established through the intraperitoneal sequential injection of rhesus rotavirus (RRV). …”
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Unveiling the Potency of Gardenia Extract Against <i>H. pylori</i>: Insights from In Vitro and In Vivo Studies by Pornpen Werawatganone, Duangporn Werawatganon, Nattida Noonak, Maneerat Chayanupatkul, Tanittha Chatsuwan, Naruemon Klaikeaw, Walaisiri Muangsiri, Prasong Siriviriyakul

“…The serum and stomach tissue of the rats were evaluated using enzyme-linked immunosorbent assay, histopathology, and immunohistochemistry. …”
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Association between Metrnl and carotid atherosclerosis in patients with type 2 diabetes mellitus by Chunmei Li, Chunmei Li, Qiongzhu Huang, Qiongzhu Huang, Yanqian Zhuang, Yanqian Zhuang, Pin Chen, Pin Chen, Yiyang Lin, Yiyang Lin

“…CIMT was measured by color doppler ultrasonography, while serum Metrnl level was measured by Enzyme-linked Immunosorbent Assay (ELISA) assay. According to the CIMT status, the subjects were divided into two groups: T2DM without atherosclerosis (DM-AS, CIMT&lt;1.0mm) and T2DM with atherosclerosis (DM+AS, CIMT ≥1.0mm or carotid plaque).ResultsSerum Metrnl level was significantly increased in DM+AS group as compared to DM-AS group (0.77 ± 0.24 vs 0.51 ± 0.28 ng/ml, P &lt;0.05). …”
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Early Changes in Tumor Perfusion from T1-Weighted Dynamic Contrast-Enhanced MRI following Neural Stem Cell-Mediated Therapy of Recurrent High-Grade Glioma Correlate with Overall Su... by Prativa Sahoo, Paul Frankel, Julie Ressler, Margarita Gutova, Alexander J. Annala, Behnam Badie, Jana Portnow, Karen S. Aboody, Massimo D’Apuzzo, Russell C. Rockne

“…The aim of this study was to correlate T1-weighted dynamic contrast-enhanced MRI- (DCE-MRI-) derived perfusion parameters with overall survival of recurrent high-grade glioma patients who received neural stem cell- (NSC-) mediated enzyme/prodrug gene therapy. Methods. A total of 12 patients were included in this retrospective study. …”
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Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats by Katarzyna Papierska, Ewa Ignatowicz, Jadwiga Jodynis-Liebert, Małgorzata Kujawska, Róża Biegańska-Marecik

“…The changes in hepatic antioxidant enzymes and plasma TEAC suggest beneficial effect of kale leaves on the antioxidant status in rats. …”
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Assembly of Genetically Engineered Ionizable Protein Nanocage-based Nanozymes for Intracellular Superoxide Scavenging by Qiqi Liu, Zhanxia Gao, Xiangyun Zhang, Qiannan Duan, Yue Zhang, Adam C. Midgley, Li Jiao, Ruming Liu, Mingsheng Zhu, Deling Kong, Jie Zhuang, Xinglu Huang

“…Abstract Nanozymes play a pivotal role in mitigating excessive oxidative stress, however, determining their specific enzyme-mimicking activities for intracellular free radical scavenging is challenging due to endo-lysosomal entrapment. …”
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Revealing novel protein interaction partners of glyphosate in Escherichia coli by Alix Sarah Aldehoff, Dominique Türkowsky, Patrick Lohmann, Masun Nabhan Homsi, Ulrike Rolle–Kampczyk, Elke Ueberham, Jörg Lehmann, Martin von Bergen, Nico Jehmlich, Sven–Bastiaan Haange

“…Extracellular riboflavin concentrations were elevated upon glyphosate exposure, while intracellular levels remained stable. Beyond the target enzyme EPSPS, thermal proteome profiling indicated an effect of glyphosate on the thermal stability of certain proteins, including AroH and ProA, indicating interactions. …”
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The assessment of marrow adiposity in type 1 diabetic rabbits through magnetic resonance spectroscopy is linked to bone resorption by Wei Li, Wei Wang, Minlan Zhang, Qi Chen, Fengyi Li, Shaojun Li

“…Biochemical analyses covered plasma glucose, enzyme levels, lipid profiles, blood urea nitrogen, creatinine levels, and markers of bone turnover. …”
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Comparison of Effect Sevoflurane-Based Anesthesia and Propofol-Based Anesthesia on the Early Postoperative Renal Function of Living Kidney Transplant Donors: A Randomized Controlle... by Cai J, Kang F, Han M, Huang X, Yan W, Wan F, Li J

“…Kidney injury marker-1 (KIM-1), interleukin-18 (IL-18) and tissue inhibitor of metalloproteinase-2 (TIMP-2) were measured by enzyme-linked immunosorbent assay. Record the cystatin C, glomerular filtration rate, urine output during perioperative period.Results: There were both increases in biomarkers of kidney injury before and 1, 2 and 6 days after the anaesthetic surgery in donors, However, no statistical differences in KIM-1 (P (0.42 pg/mL (95% CI 0.21 to 0.63 pg/mL)) vs S (0.26 pg/mL (95% CI 0.02 to 0.49 pg/mL)), − 0.16 pg/mL (95% CI − 0.48 to 0.16 pg/mL)), IL-18 (P (178.54 pg/mL (95% CI 110.15 to 24693 pg/mL)) vs S (175.86 pg/mL (95% CI 100.35 to 251.38 pg/mL)), − 2.68 pg/mL (95% CI − 105.61 to 100.25 pg/mL)), and TIMP-2 (P (12.88 ng/mL (95% CI 8.69 to 17.07 ng/mL)) vs S (14.85 ng/mL (95% CI 10.23 to 19.46 ng/mL)), 1.97 ng/mL (95% CI − 4.30 to 8.23 ng/mL)) concentration changes between the two types of anaesthesia.Conclusion: There was no difference between sevoflurane and propofol anaesthesia on postoperative changes in biomarkers of renal injury in living kidney transplant donors.Keywords: general anesthetics, sevoflurane, propofol, renal transplantation…”
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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The aims of this work were i) to determine whether it is possible to create recombinant K. phaffii K51 clones overexpressing functionally active proteinase K from T. album and ii) to analyze the enzymatic activity of the resulting recombinant enzyme. The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Cardiac Myosin-binding Protein-C as a Biomarker in the Early Diagnosis of Acute Coronary Syndrome and Differentiation of Its Types by Ali Abdul Rasool Hussein, Abdulkareem Hamadi Issa, Abbas Naji Muslem Al Shareeifi

“…For each study subject, cMybp-C and hsCT-I serum levels on admission were measured using the enzyme-linked immunosorbent assay kits. For each ACS patient, serum level of hsCTn-I level was measured 3 h after admission. …”
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Ion suppression correction and normalization for non-targeted metabolomics by Iqbal Mahmud, Bo Wei, Lucas Veillon, Lin Tan, Sara Martinez, Bao Tran, Alexander Raskind, Felice de Jong, Yiwei Liu, Jibin Ding, Yun Xiong, Wai-kin Chan, Rehan Akbani, John N. Weinstein, Chris Beecher, Philip L. Lorenzi

“…To demonstrate a routine application of the Workflow, we employ the Workflow to study ovarian cancer cell response to the enzyme-drug L-asparaginase (ASNase). The IROA-normalized data reveal significant alterations in peptide metabolism, which have not been reported previously. …”
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