Effects of Mulberry Pomace Addition and Transglutaminase Treatment on the Quality of Pasta Enriched with Antioxidants and Dietary Fiber by Thi Minh Ngoc Ta, Chi Hieu Hoang, Thao Mi Nguyen, Thi Thu Tra Tran, Nu Minh Nguyet Ton, Le Van Viet Man

“…The quality improvement of 10% mulberry pomace fortified pasta was investigated by adding a transglutaminase preparation with enzyme dosage from 0.25 to 1.00 U/g protein. The fortified pasta treated with transglutaminase at 0.50 U/g protein showed a significant improvement in chewiness, tensile strength and elongation rate but was not significantly affected in terms of the swelling index and water absorption. …”
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Photosynthetic performance and carbon metabolism in the ear organs of oats under drought stress by Jiaqi Fang, Yuan Zhan, Baowen Zhao, Yan Zhao, Youjun Chen, Qingping Zhou, Hui Wang

“…Paraffin cross-section structures were observed, and the absolute water content, photosynthetic enzyme activities, carbohydrate content, dry matter weight, and total C and total N accumulation were determined in the glumes, lemmas and flag leaves. …”
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Coproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals by Dina Aboelsoued, Nagwa I. Toaleb, Kadria N. Abdel Megeed

“…Therefore, this study aimed to evaluate an early diagnosis of cryptosporidiosis using the anti-Cryptosporidium parvum oocyst immunoglobulin IgG polyclonal antibodies (anti-C. parvum IgG PAbs)-based sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Cryptosporidium oocyst antigens in fecal samples of farm animals in Egypt. …”
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Treatment of Vascular Dementia by Using Fuyuan Capsule Combined with "Xingnao Kaiqiao" Acupuncture by LI Hongmei, WANG Xiaoqin, GUO Yang, SHI Hongxing, GUO Xiao, WANG Xue

“…The expression of protein interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) before and after treatment were determined by enzyme-linked immunoassay. Improvement in MMSE score was used as an index to assess the total effective rate in the two groups after treatment. …”
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Gap junction intercellular communications regulates activation of SARM1 and protects against axonal degeneration by Wen Jie Zhu, Jun Liu, Wan Hua Li, Zhi Ying Zhao, Chongquan Huang, Jian Yuan Yang, Hon Cheung Lee, Yong Juan Zhao

“…Abstract Sterile alpha and Toll/interleukin-1 receptor motif containing 1 (SARM1), a nicotinamide adenine dinucleotide (NAD)-utilizing enzyme, mediates axon degeneration (AxD) in various neurodegenerative diseases. …”
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Effects of hypoxia on the heart of the juvenile four-finger threadfin (Eleutheronema tetradactylum) based on physiological indicators and transcriptome analysis by Yi Lu, Eric Amenyogbe, Ye- Yang, Zhong-liang Wang, Zhong-liang Wang, Zhong-liang Wang, Jing-hui Jin, Rui-tao Xie, Eric Kwabena Droepenu, Jian-sheng Huang, Jian-sheng Huang, Jian-sheng Huang

“…Hypoxia significantly increased serum myocardial enzyme activities, including creatine kinase (CK), creatine kinase-MB isoenzyme, lactate dehydrogenase (LDH), and α-hydroxybutyrate dehydrogenase (HDBH). …”
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Dynamics of the mammalian pyruvate dehydrogenase complex revealed by in-situ structural analysis by Chen Wang, Cheng Ma, Yuanyou Xu, Shenghai Chang, Hangjun Wu, Chunlan Yan, Jinghua Chen, Yongping Wu, Shaoya An, Jiaqi Xu, Qin Han, Yujie Jiang, Zhinong Jiang, Xiakun Chu, Haichun Gao, Xing Zhang, Yunjie Chang

“…Abstract The multi-enzyme pyruvate dehydrogenase complex (PDHc) links glycolysis to the citric acid cycle and plays vital roles in metabolism, energy production, and cellular signaling. …”
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The relation of MEFV gene variants to clinical phenotype and selected laboratory markers in Egyptian patients with familial Mediterranean fever by Hala T. El-Bassyouni, Ghada Nour Eldeen, Mona F. Sokkar, Mohamed B. Taher, Mohammed M. Sayed-Ahmed, Iman I. Salama, Hala M. Raslan

“…C-reactive protein, serum amyloid A (SAA) protein and vitamin D were measured using enzyme-linked immuno-sorbent assay technique, while erythrocyte sedimentation rate was measured by Westergren method. …”
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Matrix Metalloproteinase-2 Isoforms Differ within the Aortic Wall of Ascending Aortic Aneurysms Associated with Bicuspid Aortic Valve by Ramona Schmitt, Anke Tscheuschler, Philipp Laschinski, Philipp Discher, Jana Fuchs, Fabian A. Kari

“…We therefore analyzed aTAA tissue levels of MMP-2 (matrix metalloproteinase-2) isoforms (Pro-MMP-2, active MMP-2, and total MMP-2) and tissue levels of MMP-14, TIMP-2 (tissue inhibitor of metalloproteinase-2), MMP-9, and TIMP-1 in 19 patients with BAV and 23 patients with TAV via gelatin zymography and enzyme-linked immunosorbent assay (ELISA), respectively. …”
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IFN-γ/TNF-α Synergism Induces Pro-Inflammatory Cytokine and Chemokine Production by In Vitro Canine Keratinocytes by Kyungsook Jung, Ji-Yeong Ku, Je-Seong Kwon, Gayeon Won, Hakyoung Yoon, Sang-Ik Oh, Mi Hye Kim, Chongchan Kim, Ji-Seon Yoon

“…Culture supernatants were analyzed for cytokine concentration, including chemokine ligand (CXCL) 8, IL-10, IL-6, IL-1ß, IL-12, and chemokine ligand 2 (CCL2) by enzyme-linked immunosorbent assay. CPEKs incubated with the IFN-γ/TNF-α mixture showed significantly increased IL-6 concentration. …”
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Occurrence of Aflatoxin M₁ in Raw and Processed Milk: A Contribution to Human Exposure Assessment After 12 Years of Investigation by Simona Summa, Sonia Lo Magro, Valeria Vita, Cinzia Franchino, Valentina Scopece, Pasqualino D’Antini, Marco Iammarino, Rita De Pace, Marilena Muscarella

“…A total of 1017 milk samples were analyzed first proceeding with screening analysis by enzyme-linked immunosorbent assay (ELISA), and suspected non-compliant samples (AFM₁ concentration higher than 0.042 µg/kg) were then analyzed by high performance liquid chromatographic with fluorimetric detection (HPLC/FLD) confirmation method. …”
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Enhancing phosphorus availability and dynamics in acidic soils through Rice straw biochar application: a sustainable alternative to chemical fertilizers by Wei He, Wei He, Wei He, Jun Zhang, Jun Zhang, Weichun Gao, Weichun Gao, Yifei Chen, Yifei Chen, Zheng Wei, Zheng Wei

“…The results indicate that biochar significantly increased phosphorus availability, enhanced soil enzyme activity, and boosted microbial phosphorus content, especially in the phosphorus-deficient red soil. …”
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Joint Analysis of Transcriptome and Metabolome at Different Leaf Color Stages of Cymbidium sinense 'Red Sun' by Jie GAO, Chuyan XIANG, Junhong ZHAO, Qiming PAN, Fengxi YANG

“…【Conclusion】The reduction in the content of anthocyanin synthesis precursors and the expression levels of related synthetic enzyme genes leads to the gradual disappearance of the red color in leaves of C. sinense 'Red Sun', and the high expression of photosynthetic pigment synthesis-related genes is the main reason for the leaves of C. sinense 'Red Sun' changing from yellow to green.…”
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Preassembled complexes of hAgo2 and ssRNA delivered by nanoparticles: a novel silencing gene expression approach overcoming the absence of the canonical pathway of siRNA processing... by Shimaa A. E-S. El-Sayed, Mohamed A. Rizk, Hang Li, Uday Kumar Mohanta, Iqra Zafar, Shengwei Ji, Zhuowei Ma, Thom Do, Yongchang Li, Daisuke Kondoh, Jerzy Jaroszewski, Xuenan Xuan

“…However, the primary constraint of this approach is the lack of Babesia to RNA-induced silencing complex (RISC) enzymes, making siRNA impractical. In this study, we preassembled complexes with the human enzyme argonaute 2 (hAgo2) and a small interfering RNA (siRNA)/single-stranded RNA (ssRNA) against B. gibsoni and B. microti metabolite transporters. …”
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Eugenol targeting CrtM inhibits the biosynthesis of staphyloxanthin in Staphylococcus aureus by Jiang Chang, Bo Chen, Zeqian Du, Bowen Zhao, Jiahui Li, Ziyi Li, Kannappan Arunachalam, Ting Shi, Dongqing Wei, Chunlei Shi

“…The quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM enzyme. Taken together, we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat S. aureus especially MRSA infections.…”
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Lipoprotein-Associated Phospholipase A2 Mass Level Is Increased in Elderly Subjects with Type 2 Diabetes Mellitus by J. Fortunato, V. Bláha, J. Bis, J. Št’ásek, C. Andrýs, J. Vojáček, B. Jurašková, L. Sobotka, P. Polanský, M. Brtko

“…Lp-PLA2 mass was measured using an enzyme-linked immunosorbent assay kit (USCN Life Science, China) before and 3 days after the procedure. …”
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Population Structure Based on Microsatellite Length Polymorphism, Antifungal Susceptibility Profile, and Enzymatic Activity of <i>Candida auris</i> Clinical Isolates in Russia by Ellina Oganesyan, Victoria Klimenteva, Irina Vybornova, Valentina Venchakova, Ekaterina Parshikova, Sergey Kovyrshin, Olga Orlova, Alexander Kruglov, Svetlana Gordeeva, Natalya Vasilyeva, Anastasiya Taraskina

“…Antifungal susceptibility was tested using Sensititre™ YeastOne™ plates, and hydrolytic enzyme production was measured by the plate method. …”
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Short-Term Activation of Peroxisome Proliferator-Activated Receptors α and γ Induces Tissue-Specific Effects on Lipid Metabolism and Fatty Acid Composition in Male Wistar Rats by Elin Strand, Vegard Lysne, Mari Lausund Grinna, Pavol Bohov, Asbjørn Svardal, Ottar Nygård, Rolf K. Berge, Bodil Bjørndal

“…Lipids and FA methyl esters were measured in plasma, liver, and heart and gene expression was measured in liver and adipose tissue, while enzyme activities were measured in liver. Treatment with the PPARα agonist was associated with higher liver mass relative to body weight (liver index), lower plasma, and hepatic total cholesterol, as well as lower plasma carnitine and acylcarnitines, compared with control. …”
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Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury by Vladislav N. Karkischenko, Veronika I. Skvortsova, Melik T. Gasanov, Yuriy V. Fokin, Maxim S. Nesterov, Nataliya V. Petrova, Oxana V. Alimkina, Igor A. Pomytkin

“…The mechanism of action involves preventing HMGB1’s hyperacetylation at critical lysine residues within nuclear localization sites, as well as promoting the expression of sirtuin 1 (SIRT1), an enzyme known to deacetylate HMGB1. Leytragin’s effects are mediated by opioid receptors, since naloxone, an antagonist of opioid receptors, abrogates the leytragin effect on SIRT1 expression. …”
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Colostrum-Derived Melatonin Plus PEG Microspheres Modulate the Oxidative Metabolism of Human Colostrum Phagocytes by Caroline G. Silva, Viviane F. Luz, Victor L. Nunes, Ana B. M. Verzoto, Aron C. de M. Cotrim, Wagner B. dos Santos, Eduardo L. França, Adenilda C. Honorio-França

“…Oxidative metabolism was assessed by measuring the release of the superoxide anion and superoxide enzymes. A control was conducted using commercial melatonin. …”
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