Effect of Different Anti-hypertensive Drugs on Circadian Blood Pressure Profile in Elderly Hypertensive Patients by Bingqing YU, Jinping ZHOU, Zhongxian FENG, Junxia XU

“…According to the survey of drug use, 204 elderly hypertension patients who were treated in cadre's wards of Fuzhou General Hospital were divided into five groups: 1) long-acting calcium channel blockers (CCB group)(<italic>n</italic>=50);2) angiotensin converting enzyme inhibitors (ACEIs) or angiotensinⅡreceptor blockers (ARBs)(AA group)(<italic>n</italic>=22);3) treated with joint anti-hypertensive scheme including ACEIs or ARBs (but not including diuretics)(AAND group)(<italic>n</italic>=60);4) treated with joint anti-hypertensive scheme including diuretics (but not including ACEIs or ARBs)(DNAA group)(<italic>n</italic>=19);5) treated with joint anti-hypertensive scheme including diuretics and ACEIs or ARBs group (DAA group)(<italic>n</italic>=53), to observe the profile on circadian blood pressure in each group, binary <italic>Logistic</italic> regression analysis was used to evaluate the correlation between circadian blood pressure profile and its influencing factors.Results:Compared to the CCB group, the level of all day average SBP and daytime SBP in the DAA group were both lower (all <italic>P</italic>&lt;0.01), the decreasing level of SBP at night in the DAA group was higher (<italic>P</italic>&lt;0.05). …”
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Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner I... by Lianet Lopez, Hannah Shuster-Hyman, Eden Marco, Hasna Khan, Avishai Gasner, Aleksandra Uzelac, Brandon Wyse, Poonam Mander, Mugundhine Sangaralingam, Joseph Fish, Ariel Gorodensky, Samar Mouazz, Amanda Kauffman, Denis Gallagher, Andrée Gauthier-Fisher, Clifford L. Librach

“…Serum tumor necrosis factor alpha and C-reactive protein levels were measured using enzyme-linked immunosorbent assays. Transwell coculture models were used to study the paracrine effects of multiple lines of human umbilical cord cells on human melanoma cell lines as well as breast cancer cell lines. …”
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On the Biosynthesis of Bioactive Tryptamines in Black Cohosh (<i>Actaea racemosa</i> L.) by Martin J. Spiering, James F. Parsons, Edward Eisenstein

“…Co-expression in yeast of the high-activity black cohosh TDCs with the next enzyme in serotonin biosynthesis, tryptamine 5-hydroxylase (T5H), from rice (<i>Oryza sativa</i>) resulted in the production of serotonin, whereas co-expression with low-activity TDCs did not, suggesting that TDC activity is a rate-limiting step in serotonin biosynthesis. …”
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Evaluating the Sustainability of Wheat–Maize System with a Long-Term Fertilization Experiment by Yun Shao, Jiahui An, Xueping Wang, Shouchen Ma, Ye Meng, Yang Gao, Shoutian Ma

“…The results indicated that following prolonged fertilization, the soil nutrient content, enzyme activity, and crop yield of each organic fertilizer treatment were significantly greater than those of the chemical fertilizer treatment alone, resulting in a more stable yield. …”
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Exogenous organic acids promoted phytoremediation by Hydrangea macrophylla in cadmium‑contaminated soil by Yunjing Song, Qian Cheng, Bing Zhao

“…Additionally, organic acids increased the soil pH, accompanied by changes in soil enzyme activities. 10 mmol·kg−1AA and MA reduced the available Cd concentration by 20.42 % and 31.65 %, respectively. …”
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Postdiagnostic use of antihypertensive medications and survival in colorectal, lung, corpus uteri, melanoma and kidney cancer patients with hypertension by Rūta Everatt, Irena Kuzmickienė, Birutė Brasiūnienė, Ieva Vincerževskienė, Birutė Intaitė, Saulius Cicėnas, Ingrida Lisauskienė

“…Results We found a statistically significant decrease in mortality among colorectal cancer patients who were users of angiotensin receptor blockers (ARBs) (HR: 0.68, 95% CI: 0.47–0.98) or angiotensin converting enzyme inhibitors (ACEIs) (HR: 0.69, 95% CI: 0.52–0.91). …”
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Lipid metabolic remodeling delays senescence of T cells to potentiate their immunity against solid tumors by Lu Wang, Li Ding, Gang Chen, Zhou Xu, Yanqing Zhang, Bin Deng, Xiya Wang, Yemin Xu, Mengyue Wu, Yinhe Xia, Zhennan Cao, Ruilong Song

“…Our objective is to provide a feasible strategy for enhancing T cell treatment efficacy against solid tumors.Methods In this study, liposomal arachidonyl trifluoromethyl ketone (ATK) was anchored onto the adoptive T cell surface via bioorthogonal reactions, aiming to specifically inhibit the group IVA cytosolic phospholipase A2α (cPLA2α), a key enzyme facilitating phospholipid metabolism and senescent state of T cells.Results The surface engineering exerted rare side effects on the activation and migration of T cells, but local and sustained extravasation of ATK downregulated cPLA2α expression, reprogrammed lipid metabolism, and inhibited lipid droplet accumulation. …”
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Implementation of Antigen-Based Diagnostic Assays for Detection of Histoplasmosis and Cryptococcosis among Patients with Advanced HIV in Trinidad and Tobago: A Cross-Sectional Stud... by Ayanna Sebro, Jonathan Edwards, Omar Sued, Leon-Omari Lavia, Tricia Elder, Robert Jeffrey Edwards, Patrick Eberechi Akpaka, Nadia Ram-Bhola, Roanna Morton-Williams Bynoe, Yanink Caro-Vega, Isshad John, Freddy Perez

“…Diagnostic testing was performed using the Clarus Histoplasma Galactomannan Enzyme Immunoassay (EIA), and the Immy CrAg^® LFA Cryptococcal Antigen Lateral Flow Assay on urine and blood samples, respectively. …”
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Straw return was more beneficial to improving saline soil quality and crop productivity than biochar in the short term by Ping Cong, Ping Cong, Jiashen Song, Jianxin Dong, Wenyan Su, Wenyan Su, Wenhao Feng, Hongyuan Zhang

“…The linear model further supported that soil enzyme activities are positively related to available nutrient contents and negatively correlated with salt content. …”
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Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway by Atamanalp Muhammed, Kırıcı Muammer, Köktürk Mine, Kırıcı Mahinur, Alwazeer Duried, Kocaman Esat Mahmut, Ucar Arzu, Parlak Veysel, Özcan Sinan, Alak Gonca

“…In addition, acetylcholinesterase enzyme (AChE) activity, which is important in neurotoxicity pathways in the brain, was determined. …”
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Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency by Yunting Zhou, Junming Zhou, Yumin Zhang, Jun Tang, Bo Sun, Wei Xu, Xiaohang Wang, Yang Chen, Zilin Sun

“…The intestinal morphology, intestinal permeability, and inflammatory response activation signaling pathway were assessed using histological staining, western blots, quantitative-PCR, and enzyme-linked immunosorbent assays. Results. VAD diet-fed mice displayed reduction of tissue VA levels, increased area under the curve (AUC) of glucose challenge, reduced glucose-stimulated insulin secretion, and loss of β cell mass. …”
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Inactivation of glutathione S-transferase alpha 4 blocks Enterococcus faecalis-induced bystander effect by promoting macrophage ferroptosis by Yuanyuan Ju, Chunhua Ma, Lin Huang, Yumei Tao, Tianqi Li, Haibo Li, Mark M. Huycke, Yonghong Yang, Xingmin Wang

“…Glutathione S-transferase alpha 4 (Gsta4), a specific detoxifying enzyme for 4-HNE, is overexpressed in human CRC and E. faecalis-induced murine CRC. …”
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Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab′)2-Based SPECT/CT Imaging by Dai Shi, Yiqiu Zhang, Zhan Xu, Zhan Si, Yuan Cheng, Dengfeng Cheng, Guobing Liu

“…Cet-F(ab′)2 was synthesized using immunoglobulin G-degrading enzyme of Streptococcus pyogenes (IdeS) protease and purified with protein A beads. …”
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Growth Arrest Specific Gene 6 Protein Concentration in Cerebrospinal Fluid Correlates with Relapse Severity in Multiple Sclerosis by P. P. Sainaghi, L. Collimedaglia, F. Alciato, R. Molinari, D. Sola, E. Ranza, P. Naldi, F. Monaco, M. Leone, M. Pirisi, G. C. Avanzi

“…Gas6 was measured (CSF, plasma) by in-house-enzyme-linked immunoassay (ELISA). Results. Higher CSF Gas6 concentrations were observed in relapses lasting ≤60 days (8.7 ± 3.9 ng/mL) versus >60 days (6.5 ± 2.6) or controls (6.5 ± 2.4; P=0.05), with ≤2 FS involved (8.5 ± 3.8) versus >2 FS (5.6 ± 2.5) (P<0.05) and EDSS change ≤2.5 points (8.8 ± 3.7) versus >2.5 (6.5 ± 3.5) (P=0.04). …”
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The influence of external factors on the development of olfactory disorders in patients with the coronavirus disease 2019: an immunomorphological assessment by I.S. Shponka, O.M. Usova

“…Mucosal samples were stained with standard hematoxylin and eosin and using an immunohistochemical method with antibodies to neuron-specific beta-III tubulin (TuJ-1), olfactory marker protein (OMP) and angiotensin-converting enzyme (ACE-2). The distribution of expression variants of receptors to OMP, TuJ-1 and ACE-2 in sections with signs of structural rearrangement and without signs of structural rearrangement at a magnification of 200x showed a significant difference (p<0.05): the number of OMP-positive cells in sections of the olfactory epithelium of the first group on average was 43.5 in the field of view (16.0-59.0), TuJ-1-positive cells in these sections – 44.5 in the field of view (17.0-61.0), ACE-2-positive cells – 37 in the field of view (14.0-55.0), and in the samples of the olfactory epithelium of the second group, the expression of receptors to OMP was only partially positive (18 (12.0-25.0) cells), TuJ-1 – 17.5 (14.0-24.0) cells, ACE-2 – 14 (9.0-18.0) cells. …”
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Diabetic Nephropathy Induced by Increased Ace Gene Dosage Is Associated with High Renal Levels of Angiotensin (1–7) and Bradykinin by Nádia Bertoncello, Roseli Peres Moreira, Danielle Yuri Arita, Danielle S. Aragão, Ingrid Kazue Mizuno Watanabe, Patricia S. Dantas, Ralmony Santos, Rodolfo Mattar-Rosa, Rodrigo Yokota, Tatiana Sousa Cunha, Dulce Elena Casarini

“…The susceptibility to the development of DN in 3 copies of animals is associated with an imbalance in activity of RAS enzymes leading to increased synthesis of Ang II and Ang-(1–7). …”
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Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type <i>Acinetobacter baumannii</i> in Mouse Sepsis and Burn Skin Infection Models by Alexander I. Borzilov, Nikolay V. Volozhantsev, Olga V. Korobova, Lyubov V. Kolupaeva, Evgenia S. Pereskokova, Tatiana I. Kombarova, Mikhail M. Shneider, Konstantin A. Miroshnikov, Ivan A. Dyatlov, Anastasia V. Popova

“…In the case of delayed treatment, mouse survival decreased to 70% when injected with the phage and to 40% when treated with the recombinant enzyme. In the mouse burn skin infection model, the number of <i>A. baumannii</i> cells on the surface of the wound and in the deep layers of the skin decreased by several-fold after treatment with both the K9-specific phage and the recombinant depolymerase. …”
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Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin by Eman Ibrahem, Ali Osman, Hefnawy Taha, Mohamed F. Abo El-Maati, Basel Sitohy, Basel Sitohy, Mahmoud Sitohy

“…IntroductionThis study investigated the tryptic hydrolysis of β-lactoglobulin (BLG) for 30, 60, 90, and 120 min at 1/200 E/S (enzyme/substrate ratio, w/w) to prepare potentially anticarcinogenic peptides.MethodsThe properties of hydrolysates were characterized, including degree of hydrolysis, free amino acids, SDS-PAGE, FTIR, and antioxidant activity employing DPPH-assay, β-carotene/linoleic acid, and FRAP assay.ResultsBLG tryptic hydrolysate produced after 60 min hydrolysis recorded the highest antioxidant activity, and LCMS analysis revealed 162 peptides of molecular masses ranging from 800 to 5671Da, most of them are of hydrophobic nature. …”
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Fc-binding nanodisc restores antiviral efficacy of antibodies with reduced neutralizing effects against evolving SARS-CoV-2 variants by Jaehyeon Hwang, Soyun Choi, Beom Kyu Kim, Sumin Son, Jeong Hyeon Yoon, Kyung Won Kim, Wonbeom Park, Hyunjoo Choo, Suhyun Kim, Soomin Kim, Seokhyeon Yu, Sangwon Jung, Sang Taek Jung, Min-Suk Song, Sang Jick Kim, Dae-Hyuk Kweon

“…Furthermore, the Fc-binding nanodisc significantly boosted the antiviral efficacy of the soluble angiotensin-converting enzyme 2 (sACE2) Fc fusion protein, which possesses broad but modest antiviral activity. …”
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Oxidative Stress and Risk of Dementia in Older Patients with Depression: A Longitudinal Cohort Study Using Plasma Biomarkers by Yoo-Jin Jang, Min-Ji Kim, Su-Jin Lee, Shinn-Won Lim, Doh-Kwan Kim

“…Biomarkers, such as nitrotyrosine, protein carbonyl, F2-isoprostanes, malondialdehyde, 4-hydroxynonenal, and 8-hydroxy-2′-deoxyguanosine, were collected at baseline and measured using an enzyme-linked immunosorbent assay. AD conversion was determined using comprehensive neuropsychological assessment. …”
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