Characteristics of metabolic syndrome in the servicemen with erosive esophagitis in wartime conditions by L.M. Mosiychuk, O.M. Shevtsova, O.M. Tatarchuk, O.P. Petishko

“…Insulin resistance was evaluated using the HOMA-IR index, for the calculation of which the level of insulin was determined by enzyme-linked immunosorbent assay systems of the Xema company (Ukraine) on the Stat Fax 303 Plus analyzer (USA). …”
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Correlation of irisin with lipid profile in type 2 diabetes mellitus by Wahengbam Diana Devi, Oinam Prabita Devi, Ursula Khwairakpam, Takhelmayum Paras Singh

“…Materials and Methods: We conducted a case–control study on 40 T2DM patients and 40 healthy controls. Enzyme-linked immunosorbent assay was employed for the estimation of serum irisin level, estimation of FBS by glucose oxidase-phenol 4 amino phenazone method (GOD/PAP), and lipid profile by enzymatic colorimetric method. …”
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Omentin-1, a Protective Adipokine for Irritable Bowel Syndrome by Gao Y, Chen C, Huang X, Liu Y, Zhou Z, Pan Y

“…Our study aimed to investigate associations of omentin-1 (a newly discovered adipokine) with IBS.Methods: In the current study, serum levels of omentin-1 were measured in 209 patients with IBS (including three subtypes) and 188 healthy controls by enzyme-linked immunosorbent assay (ELISA). The somatic symptoms of IBS were determined by the 5-item IBS symptoms severity score (IBS-SSS), quality of life (QOL) by 34-item IBS-QOL questionnaire, and psychological disorders by Patient Health Questionnaire (PHQ-9), Hospital Anxiety and Depression Scale (HADS), Visceral Sensitivity Index (VSI). …”
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LIN28A-dependent lncRNA NEAT1 aggravates sepsis-induced acute respiratory distress syndrome through destabilizing ACE2 mRNA by RNA methylation by Jun Liu, Xiang Li, Peng Yang, Yufeng He, Weilong Hong, Yawei Feng, Zhiqiang Ye

“…Results This study revealed that long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) aggravated lung injury through suppressing angiotensin-converting enzyme 2 (ACE2) in sepsis-induced ARDS models in vitro and in vivo. …”
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Optimization of the extraction methods and evaluation of the hypoglycemic effect of Adhatoda Zeylanica extracts on artificially induced diabetic mice by Md Fahim Ahmed, Khalid Hasan Raby, Nishat Tasnim, Md Tariful Islam, Mahbub Chowdhury, Zarin Tasnim Juthi, Md Ashik Mia, Lubna Jahan, A.K.M. Zakir Hossain, Shoeb Ahmed

“…This study explored four extraction methods, including reflux distillation (RD), ultrasound assisted extraction (UAE), microwave assisted extraction (MAE), and enzyme assisted extraction (EAE) to optimize the yield of crude leaf extract and vasicine from Adhatoda zeylanica. …”
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Detection of nontoxic BoNT/A levels in post-facial Botox injection breastmilk by Helene Gu, Zhenyu Xu, Renata Koviazina, Pengcheng Tan, Changcheng Zheng, Ferdinand Kappes, Domna G. Kotsifaki, Fangrong Shen, Anastasia Tsigkou

“…BoNT/A concentrations in samples were detected using standard Enzyme-Linked Immunosorbent Assay (ELISA), unreduced and reduced Western Blotting, confocal micro-Raman Spectroscopy, and Mass Spectrometry (LC-MS). …”
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Salt Stress Enhanced Bioactivity of Quinoa Leaf Extracts: An In Vitro and In Silico Study of Acetylcholinesterase and Tyrosinase Inhibition for Sustainable Drug Development by Narmine Slimani, Soumaya Arraouadi, Hafedh Hajlaoui, Antonio Cid-Samamed, Mohamed Ali Borgi, Mejdi Snoussi

“…<b>Conclusions:</b> Salt stress not only promotes the production of bioactive phenolic compounds in quinoa leaves but also enhances their inhibitory effects on key enzymes associated with neurodegenerative and pigmentary disorders. …”
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Lack of intracranial atherosclerosis in various atherosclerotic mouse models by Diewertje I Bink, Katja Ritz, Claire Mackaaij, Olga Stam, Sanny Scheffer, Mark R Mizee, Hanneke J Ploegmakers, Bert J van het Hof, Onno J de Boer, Judith C Sluimer, Guido R Y De Meyer, Louise van der Weerd, Helga E de Vries, Mat J A P Daemen

“…Compared to extracranial sections, intracranially the number of elastic layers decreased, tight junction markers increased, and antioxidant enzyme heme oxygenase (HO)-1 increased. Higher HO-1 expression was also shown in human intracranial arteries. …”
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Prevalidation of an ELISA for Detection of a New Clinical Entity: Leishmania donovani-Induced Cutaneous Leishmaniasis by Bhagya Deepachandi, Sudath Weerasinghe, Himali Gunathilake, Thisira P. Andrahennadi, Mahendra N. Wickramanayake, Shantha Siri, Vishvanath Chandrasekharan, Preethi Soysa, Yamuna Siriwardana

“…A suitable serology-based assay is an essential primary step in such a study. An indirect enzyme-linked immunosorbent assay (ELISA) based on Leishmania promastigote crude antigen (Ag) was designed and optimized in order to utilize in further serological studies on this new clinical entity. …”
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Combined oven/freeze drying as a cost and energy-efficient drying method for preserving quality attributes and volatile compounds of carrot slices by Zobabalo Progress Mina, Tafadzwa Kaseke, Tafadzwa Kaseke, Tobi Fadiji, Yardjouma Silue, Olaniyi Amos Fawole, Olaniyi Amos Fawole

“…Combined drying represents a novel approach that addresses the shortcomings of conventional methods by balancing energy consumption, cost, and product quality.MethodsThis study evaluated the impact of combining oven drying (OD) with freeze-drying (FD) on drying behavior, energy requirements, costs, enzyme activity, and the physicochemical and sensory properties of dried carrots. …”
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Correlation between Apelin and Some Angiogenic Factors in the Pathogenesis of Preeclampsia: Apelin-13 as Novel Drug for Treating Preeclampsia and Its Physiological Effects on Place... by Reham Z. Hamza, Abdel Aziz A. Diab, Mansour H. Zahra, Ali K. Asalah, Suzan M. M. Moursi, Najah M. Al-Baqami, Fawziah A. Al-Salmi, Mai S. Attia

“…Moreover, serum apelin, placental growth factor (PLGF), vascular endothelial growth factor (VEGF), soluble fms-like tyrosine kinase-1 (sFlt-1), soluble endoglin (sEng), interferon-gamma (IFN-γ), and interleukin-10 (IL-10) levels and serum superoxide dismutase enzyme (SOD) and catalase (CAT) activities of all groups were estimated at the end of experiment. …”
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Lower Serum IL-10 Linked to Oral Manifestations in Diabetes Patients by Nur'aeny N, Widiasta A, Novianti Y, Zakiawati D, Pratidina NB, Sufiawati I

“…Serum levels of IL-10 were measured using an enzyme-linked immunosorbent assay (ELISA) kit.Results: Among the 35 DM patients with oral manifestations, xerostomia was the most common (80%). …”
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Longitudinal analysis of academic stress and its effects on salivary cortisol, alpha-amylase, and academic outcomes: Study protocol. by Juan Luis Castillo-Navarrete, Alejandra Guzmán-Castillo, Claudio Bustos

“…Students will also collect saliva samples three times a week to measure cortisol levels, and alpha-amylase enzyme activity.<h4>Statistical analysis</h4>(i) Descriptive analysis of variables will be performed using measures of central tendency and dispersion for continuous variables and frequencies and percentages for categorical variables. …”
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Sleep-Aiding Effect of Casein-Derived Peptide TPTLN and Its Underlying Mechanism by DENG Shijie, LIU Ling, HUANG Ju, FENG Fengqin, ZHAO Minjie, DU Juan

“…Following 4 more days of administration, the mice were euthanized and dissected to collect blood and tissue samples for analysis. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), 5-hydroxyindoleacetic acid (5-HIAA), glutamate (Glu), β-endorphin (β-EP), cortisol, norepinephrine (NE) in the serum and brain tissue, and the GABA/Glu ratio was evaluated. real-time quantitative polymerase chain reaction (qPCR) was used to detect the effect of TPTLN on the mRNA relative expression levels of γ-aminobutyric acid A receptor α1 (GABAA-α1) and 5-hydroxytryptamine receptor 1A (5-HT1A) in the hypothalamus. …”
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Prohexadione Calcium Improves Rice Yield Under Salt Stress by Regulating Source–Sink Relationships During the Filling Period by Rui Deng, Dianfeng Zheng, Naijie Feng, Aaqil Khan, Jianqin Zhang, Zhiyuan Sun, Jiahuan Li, Jian Xiong, Linchong Ding, Xiaohui Yang, Zihui Huang, Yuecen Liao

“…In this study, we used Guanghong 3 (salt-tolerant variety) and Huanghuazhan (salt-sensitive variety) as experimental materials to investigate the dynamic changes in the synthesis and partitioning of nonstructural carbohydrates among source–sink, the dynamic changes in related enzyme activities, the effects of the source–sink metabolism on yield in rice under salt stress and the effect of Pro-Ca during the filling period. …”
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Effect of Swimming on Gut Microbiota and Inflammation in Colorectal Cancer Cells Transplanted Mice by LI Jiapeng, XIE Qiurong, WEN Ying, CHEN Haichun, PENG Jun, SHEN Aling

“…The functional enrichment of gut microbiota in mice was evaluated by KEGG to compare the differences of metabolic pathways of functional genes in the intestinal flora between the two groups. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of TNF-α, IL-1β and IL-10 in serum of mice. …”
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To Develop Biomarkers for Diabetic Nephropathy Based on Genes Related to Fibrosis and Propionate Metabolism and Their Functional Validation by Sha Li, Jingshan Chen, Wenjing Zhou, Yonglan Liu, Di Zhang, Qian Yang, Yuerong Feng, Chunli Cha, Li Li, Guoyong He, Jun Li

“…Seven biomarkers, namely, SLC37A4, ACOX2, GPD1, angiotensin-converting enzyme 2 (ACE2), SLC9A3, AGT, and PLG, were acquired via RF and RFE analyses, and they were found to be involved in various mechanisms such as glomerulus development, fatty acid metabolism, and peroxisome. …”
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Genetically edited human placental organoids cast new light on the role of ACE2 by Anya L. Arthurs, Bianca Dietrich, Martin Knöfler, Caleb J. Lushington, Paul Q. Thomas, Fatwa Adikusuma, Jessica M. Williamson, Susan Babikha, Tyla Damhuis, Tanja Jankovic-Karasoulos, Melanie D. Smith, Kirsty G. Pringle, Claire T. Roberts

“…Despite increased expression of ACE2 protein, ACE2 enzyme activity was significantly decreased in ACE2 rs2074192 TT placental organoids. …”
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Response mechanism of ethanol-tolerant Saccharomyces cerevisiae strain ES-42 to increased ethanol during continuous ethanol fermentation by Xue-Xue Ji, Quan Zhang, Bai-Xue Yang, Qing-Ran Song, Zhao-Yong Sun, Cai-Yun Xie, Yue-Qin Tang

“…At lower ethanol concentrations (nearly 70 g/L), genes involved in mitochondrial ribosomes, oxidative phosphorylation, the tricarboxylic acid (TCA) cycle, antioxidant enzymes, ergosterol synthesis, and glycerol biosynthesis were specifically upregulated compared to those at 60 g/L. …”
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Organic Amendments Enhance the Remediation Potential of Economically Important Crops in Weakly Alkaline Heavy Metal-Contaminated Bauxite Residues by Xingfeng Zhang, Qiankui Yu, Bo Gao, Maosheng Hu, Hongxu Chen, Yexi Liang, Haifeng Yi

“…Additionally, the use of organic amendments enhanced BR enzyme activity, with microbial fertilizer demonstrating a substantial increase in BR invertase activity from 131.49 to 687.61%. …”
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