Complete Genome Sequence and Comparative Genome Analysis of Variovorax sp. Strains PAMC28711, PAMC26660, and PAMC28562 and Trehalose Metabolic Pathways in Antarctica Isolates by Prasansah Shrestha, Jayram Karmacharya, So-Ra Han, Jun Hyuck Lee, Hyun Park, Tae-Jin Oh

“…Likewise, CAZyme annotation (dbCAN2 Meta server) was performed to predict the CAZyme family responsible for trehalose biosynthesis and degradation enzymes. Trehalose has been found to respond to osmotic stress and extreme temperatures. …”
Get full text

PMA1-containing extracellular vesicles of Candida albicans triggers immune responses and colitis progression by Zhen Xu, Shuping Qiao, Zelin Wang, Chen Peng, Yayi Hou, Baorui Liu, Guochun Cao, Tingting Wang

“…CARD9 deficiency led to decreased enzyme activity of GAPDH and decreased glycolysis of DCs. …”
Get full text

Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice by Hiroki Goto, Aiko Inoue, Limei Piao, Lina Hu, Zhe Huang, Xiangkun Meng, Yusuke Suzuki, Hiroyuki Umegaki, Masafumi Kuzuya, Xian Wu Cheng

“…On day 3 postinjury, rPLF-1 increased the levels of proteins or genes for p-Akt, p-mTOR, p-GSK3α/β, p-Erk1/2, p-p38MAPK, interleukin-10, Pax7, MyoD, and Cyclin B1, and it increased the numbers of CD34+/integrin-α7+ muscle stem cells and proliferating cells in the muscles and/or bone marrow of CTX mice. An enzyme-linked immunosorbent assay revealed that rPLF-1 suppressed the levels of plasma tumor necrosis factor-α and interleukin-1β in CTX mice. …”
Get full text

Association of leptin receptor Gln223Arg genetic polymorphism with obesity and non-alcoholic fatty liver disease by A. V. Morozova, N. V. Mal'tseva, Y. A. Gorbatovsky, O. F. Lykova, S. V. Arkhipova

“…Cholesterol and triglycerides serum levels were estimated by enzyme colorimetric method, leptin concentration — by immunoenzyme method. …”
Get full text

Dipeptidyl Peptidase 4 Activity Is Related to Body Composition, Measures of Adiposity, and Insulin Resistance in Subjects with Excessive Adiposity and Different Degrees of Glucose... by Wellington S. Silva Júnior, Maria das Graças C. Souza, José Firmino Nogueira Neto, Eliete Bouskela, Luiz Guilherme Kraemer-Aguiar

“…Background. The enzyme dipeptidyl peptidase 4 (DPP4) has been recently recognized as an adipo-myokine. …”
Get full text

Serum choline, leptin and interleukin-6 levels in fibromyalgia syndrome-induced pain: a case–control study by Elif Baris, Izel Topaloglu, Elif Akalin, Emre Hamurtekin, Seray Kabaran, Ayse Gelal, Reyhan Ucku, Mualla Aylin Arici

“…Serum choline, leptin and (interleukin)IL-6 levels were measured from fasting blood samples of volunteers with enzyme-linked immunosorbent assays (ELISA). Results All FMS patients (n = 38) and healthy volunteers (n = 38) were female. …”
Get full text

Synergistic attenuation of complete freund’s adjuvant-induced inflammation in mice using shinbaro-pelubiprofen: a novel therapeutic complex by Hyunseong Kim, Jin Young Hong, Wan-Jin Jeon, Hyun Kim, Changhwan Yeo, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha

“…Viability and cytotoxicity assays, immunohistochemistry, enzyme-linked immunosorbent assays, real-time polymerase chain reaction assays, nociception assays, writhing tests, and blood biochemical analyses were performed. …”
Get full text

Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation by Yanqin Wang, Xinxin Li, Xiaoyun Xing, Haibo Xue, Ruiqun Qi, Hong Ji, Lei Ma

“…Quantitative real-time RT-PCR detected Hes1 mRNA expression. Enzyme-linked immunosorbent assay and western blot measured IL-17A serum concentration and protein expression. …”
Get full text

Effect of non-surgical periodontal therapy on salivary and gingival crevicular fluid concentration of visfatin in periodontal health and disease by Hira Ismail, Anjani Kumar Pathak, Nand Lal, Pavitra Rastogi, Kaleem Ahmad, Mohd. Aamir Khan

“…It serves the roles of a cytokine, an enzyme involved in energy metabolism, and a growth factor. …”
Get full text

<i>Weizmannia coagulans</i> BC99 Attenuates Oxidative Stress Induced by Acute Alcoholic Liver Injury via Nrf2/SKN-1 Pathway and Liver Metabolism Regulation by Ying Wu, Cheng Li, Yinyin Gao, Jie Zhang, Yao Dong, Lina Zhao, Yuwan Li, Shaobin Gu

“…However, intervention with BC99 suppressed CYP2E1 expression and enhanced antioxidant enzyme activities through the Nrf2/SKN-1 pathway, thereby alleviating oxidative stress. …”
Get full text

Associations of CAG repeat polymorphism in the androgen receptor gene with steroid hormone levels and anthropometrics among men: the role of the ethnic factor by L. V. Osadchuk, G. V. Vasiliev, A. V. Osadchuk

“…Serum testosterone and estradiol were determined by enzyme immunoassay. Genotyping of the AR CAG repeats was performed using fragment analysis and capillary electrophoresis. …”
Get full text

A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance a... by Ning Gan, Ping Xiong, Ji Wang, Tianhua Li, Futao Hu, Yuting Cao, Lei Zheng

“…The amount of Δf was proportional to (CRP) from 0.003 to 200 ng mL−1 with a low detection limit of 1 pg mL−1. Compared with the enzyme-linked immunosorbent assay (ELISA), the immunoassay shows greatly improved sensitivity due to the significant amount of HRP labeled on signal tag. …”
Get full text

Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…The expression of inflammatory factors, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and interleukin (IL)-8, was evaluated using enzyme-linked immunosorbent assay (ELISA). Dual-luciferase reporter assays validated the targeting relationship between miR-182-5p and FGF9. …”
Get full text

Exploring diazotrophic diversity: unveiling Nif core distribution and evolutionary patterns in nitrogen-fixing organisms by Bruno Thiago de Lima Nichio, Roxana Beatriz Ribeiro Chaves, Fábio de Oliveira Pedrosa, Roberto Tadeu Raittz

“…Abstract Background Diazotrophs carry out biological nitrogen fixation (BNF) using the nitrogenase enzyme complex (NEC), which relies on nitrogenase encoded by nif genes. …”
Get full text

Peroxiredoxin 6 maintains mitochondrial homeostasis and promotes tumor progression through ROS/JNK/p38 MAPK signaling pathway in multiple myeloma by Dandan Gao, Yang Lv, Fei Hong, Dong Wu, Ting Wang, Gongzhizi Gao, Zujie Lin, Ruoyu Yang, Jinsong Hu, Aili He, Pengyu Zhang

“…Mechanically, PRDX6 serves as an anti-oxidative enzyme, and its deficiency led to over-accumulation of reactive oxygen species (ROS), resulting in oxidative stress, following the activation of MAPK signaling pathway, which manifested as phosphorylation of JNK and p38. …”
Get full text

Apigenin suppresses osteoarthritis progression by inhibiting pyroptosis via SIRT1/mTOR pathway-mediated mitophagy by Jiahao Ying, Shijie Li, Anna Ying, Yindong Ding, Xiaoyin Zhu, Jiahao Lin, Kehe Yu

“…Techniques such as Western blot analysis, Enzyme-linked Immunosorbent Assay (ELISA), and immunofluorescence staining were applied to examine protein level changes in vitro experiments. …”
Get full text

N-acetylglutamate synthetase deficiency - literature review by Krystian Wdowiak, Agnieszka Maciocha, Julia Wąż, Aleksandra Witas, Justyna Drogoń, Weronika Chachaj, Natalia Szymkowiak, Ewa Gardocka

“…Brief Description of the State of Knowledge:  Symptoms of NAGSD typically appear in the neonatal period. The NAGS enzyme is essential for catalyzing the synthesis of N-acetylglutamate, an allosteric activator of carbamoylphosphate synthetase 1 (CPS1), crucial for the urea cycle. …”
Get full text

Essential Oils from Citrus Peels Promote Calcium Overload-Induced Calcicoptosis in U251 Cells by Yurong Li, Juanjuan Wei, Zimao Ye, Chen Ji, Wenji Li, Li Xu, Zhiqin Zhou

“…Additionally, the antioxidant enzyme system (SOD, POD, CAT, and GR) and the nonenzymatic defense system (GSH) were inhibited, leading to an increase in lipid peroxidation. qRT–PCR indicated the significant upregulation of intracellular calcium ion signaling pathways and the upregulation of mitochondrial apoptosis-related genes. …”
Get full text

Tetramycin ameliorates tebuconazole·azoxystrobin to control leaf spot and viral diseases of Taizishen by Bing Tian, Bing Tian, Chenglin Tang, Jiaqi Liu, Boya Jin, Cheng Zhang

“…Concurrently, their combined application could notably enhance total soluble flavonoids, total soluble phenols, protective enzyme activity, IC, intracellular water metabolism, nutrient transport, and metabolic activity, while reducing its MDA, IR, IZ, IXc, and IXL. …”
Get full text

Anti-motilities and anti-biofilm effects of Ageratum conyzoides L. methanol extract by Eli Compaoré, Moussa Compaoré, Vincent Ouédraogo, Ablassé Rouamba, Alimata Bancé, Mignini R. Dofini, Martin Kiendrebeogo

“…The anti-biofilm effect was conducted in microtiter plates using the crystal violet method. The antioxidant and enzyme inhibition activities were evaluated using 2,2-diphényl-1-picrylhydrazyl; 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulfonic acid), Ferric Reducing Antioxidant Power and conducting lipoxygenase test. …”
Get full text