Hybrid Approach in the Analysis of Bovine Milk Protein Hydrolysates as a Source of Peptides Containing Di- and Tripeptide Bitterness Indicators by Anna Iwaniak, Piotr Minkiewicz, Monika Hrynkiewicz, Justyna Bucholska, Małgorzata Darewicz

“…Hydrolysis was simulated using the BIOPEP-UWM “Enzyme(s) action” tool. Potentially released peptides (called parent peptides) were analyzed for the presence of shorter peptide regions with bitter taste. …”
Get full text

Extraction of Saponins from Xanthoceras sorbifolium Bunge Leaves Using Deep Eutectic Solvents and Their Inhibitory Activity against Postharvest Pathogenic Fungi by Xue LI, Xinyu CHU, Erzheng SU, Jiahong WANG

“…Among the pathogenic fungi studied, the spore germination of Rhizopus oryzae was inhibited with the best effect, the inhibition rate reached 78.76%±2.56%, and XLs saponin inhibited the growth of the fungus by suppressing the antioxidant enzyme activity. These results suggest promising applications for XLs saponins in the postharvest preservation of fruits and vegetables.…”
Get full text

A study examining the effects of changed rules of wrestling competitions on the muscular damage levels of the elite wrestlers by C. Berkan Alpay, Hasan Eker, Ozkan Isık, Serkan Hazar

“…For looking at the levels of Creatine Kinase (CK), Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT), serums were obtained by centrifuging the blood samples those taken to determine the levels of the skeletal muscle damages of the wrestlers at 1000 RPM for 20 minutes. The enzyme levels of the blood samples (the serum of which was decomposed) were determined by the SIEMENS ADVIA biochemistry auto-analyzer using the SIEMENS kit. …”
Get full text

CLINICAL AND LABORATORY FEATURES OF LYME ARTHRITIS AND ARTHRITIDES OF OTHER ETIOLOGIES IN CHILDREN OF LVIV REGION by Halyna Lytvyn, Natella Basa

“…The laboratory criterion for Lyme arthritis is positive IgG to B. burgdorferi in enzyme immunoassays and immunoblot analyses. Significantly (p=0.02) higher levels of IgG >200 IU/ml were more often found in 42.9% of patients with Lyme arthritis. …”
Get full text

Surface topography modulates initial platelet adhesion to titanium substrata by Cecilia Yan Guo, Raymond Mo, Hugh Kim

“…In addition, the secretion of critical molecules stored in platelet granules (platelet factor 4, PF4; soluble P-selectin, sCD62P; transforming growth factor-beta1, TGF-β1; platelet-derived growth factor-AB, PDGF-AB) was measured by enzyme-linked immunosorbent assay (ELISA) analysis of the supernatants. …”
Get full text

Evaluation of TNF-α, IL-10 and IL-6 Cytokine Production and Their Correlation with Genotype Variants amongst Tuberculosis Patients and Their Household Contacts. by Lavanya Joshi, Meenakshi Ponnana, Ramya Sivangala, Lakshmi Kiran Chelluri, Prathiba Nallari, Sitaramaraju Penmetsa, Vijayalakshmi Valluri, Sumanlatha Gaddam

“…<h4>Methods</h4>The cytokine levels were estimated in serum by enzyme-linked immunosorbent assay (ELISA) and their polymorphisms were studied by amplification refractory mutation system polymerase chain reaction (ARMs PCR) in active pulmonary tuberculosis patients (APTB = 150), household contacts (HHC = 190), and healthy controls (HC = 150).…”
Get full text

Pengaruh Timbal (Pb) Terhadap Kadar MDA Serum Tikus Putih Jantan by Endrinaldi ., Asterina .

“…Pb toxicityinhibitsan enzyme that acts as an antioxidant and liver cell damage.…”
Get full text

Typical and atypical metabolic characteristics of three iridaceae isoflavone components: in vitro and in silico studies by Jifeng Gu, Jifeng Gu, Huishan Zhang, Mei Wang, Yuyang Zhou, Yuyang Zhou, Zhipeng Deng, Rong Shi

“…However, the metabolic characteristics of isoflavone aglycones remain unclear.MethodsIn this study, human liver microsomes (HLMs) and Cytochrome P450 (CYP) recombinant enzymes were used to study the metabolic stability, identify the metabolic pathways and enzyme kinetics of these three components, and elucidate their possible binding sites through molecular docking.ResultsWhen tectorigenin, irigenin, and irisflorentin were co-incubated with HLMs and CYP recombinant enzymes, hydroxylation metabolite for tectorigenin, demethylated metabolite for irigenin, and 6,7-dihydroxy-5,3′,4′,5′-tetramethoxy isoflavone originating from irisflorentin were identified. …”
Get full text

Cryoablation combined with dual immune checkpoint blockade enhances antitumor efficacy in hepatocellular carcinoma model mice by Jun Gu, Zepeng Yu, Xiangxiang Tang, Wenying Chen, Xuedong Deng, Xiaoli Zhu

“…The numbers of infiltrating T lymphocytes were checked by immunohistochemistry, and the levels of T-cell-associated cytokines were detected by Quantitative real-time Polymerase Chain Reaction (qRT-PCR) assays and Enzyme-Linked Immunosorbent Assays (ELISA) assays.Results Cryo + ICB inhibited the growth of right untreated tumors, promoted tumor cell apoptosis, and prolonged the survival time of mice. …”
Get full text

Isolation and identification of novel casein-derived bioactive peptides and potential functions in fermented casein with Lactobacillus helveticus by Mengzhu Fan, Tingting Guo, Wanru Li, Jing Chen, Fushuo Li, Chao Wang, Yi Shi, David Xi-an Li, Shaohui Zhang

“…Forty-four of the identified peptides were previously described as bioactive, including angiotensin-converting enzyme (ACE)-inhibitory, antioxidant, immunomodulating, antimicrobial, DPP-IV inhibitory, antiamnesic and anticancer effects and so on. …”
Get full text

Nanoparticles for Biomedical Use Derived from Natural Biomolecules: Tannic Acid and Arginine by Mehtap Sahiner, Selin S. Suner, Nurettin Sahiner

“…The α-glucosidase inhibition of p(TA-co-ARG) particles (2 mg/mL) 1:1 and 1:2 mole ratios revealed significant enzyme inhibition ability, e.g., 91.3 ± 3.1% and 77.6 ± 12.0%. …”
Get full text

Synthesis and Evaluation of N-[1-(((3,4-Diphenylthiazol-2(3H)-ylidene)amino)methyl)cyclopentyl]acetamide Derivatives for the Treatment of Diseases Belonging to MAOs by Gülhan Turan-Zitouni, Aouatef Tabbi, Weiam Hussein, Abdullah Burak Karaduman, Begüm Nurpelin Sağlık, Yusuf Özkay

“…The inhibitory activity of compounds (4a-4i) against hMAO-A and hMAO-B enzymes was elucidated by using in vitro fluorometric method using Amplex Red® reagent. …”
Get full text

The effect of Tai Chi on plasma homocysteine in 1176 adults: a propensity score matching-based study by Yingchun Gu, Juncai Bai, Yang Li, Ling Han, Dongwei Wang

“…Plasma Hcy in the two groups was determined by using the enzyme-linked immunosorbent assay (ELISA) Kit for Hcy. …”
Get full text

Interferon-γ and Interleukin-10 Responses during Clinical Malaria Episodes in Infants Aged 0–2 Years Prenatally Exposed to Plasmodium falciparum: Tanzanian Birth Cohort by Boniphace Sylvester, Dinah B. Gasarasi, Said Aboud, Donath Tarimo, Siriel Masawe, Rose Mpembeni, Göte Swedberg

“…This prospective cohort study involved 215 infants aged 0-2 years born to mothers with or without placental malaria. Enzyme-linked immunosorbent assay (ELISA) was used to determine levels of IL-10 and IFN-γ in infants and detect IgM in cord blood. …”
Get full text

Occupationally Acquired American Cutaneous Leishmaniasis by Maria Edileuza Felinto de Brito, Maria Sandra Andrade, Éricka Lima de Almeida, Ângela Cristina Rapela Medeiros, Roberto Pereira Werkhäuser, Ana Isabele Freitas de Araújo, Sinval Pinto Brandão-Filho, Alzira Maria Paiva de Almeida, Eduardo Henrique Gomes Rodrigues

“…One isolate was obtained by culture (from patient 2 biopsy samples) and characterized as Leishmania (Viannia) naiffi through an indirect immunofluorescence assay (IFA) with species-specific monoclonal antibodies (mAbs) and by multilocus enzyme electrophoresis (MLEE). Patients were successfully treated with N-methyl-glucamine. …”
Get full text

Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli by Xiaofeng Liu, Jingru Zhou, Muhammad Zeeshan Ul Haq, Zhineng Fu, Guangtao Gu, Jing Yu, Ya Liu, Dongmei Yang, Huageng Yang, Yougen Wu

“…Under p-HBA stress, silencing the PcCDPK5 homologous gene in Nicotiana tabacum leads to decreased antioxidant enzyme activity and increased malondialdehyde (MDA) content, significantly accumulating reactive oxygen species (ROS) and affecting normal plant growth. …”
Get full text

High-quality instant black tea manufactured using fresh tea leaves by two-stage submerged enzymatic processing by Junhao Kong, Xiufang Yang, Xiaobo Zuo, Xiaoqin Su, Bing Hu, Xinle Liang

“…The changes of aroma profiles and chemical constituents including theaflavins (TFs), thearubigins (TRs), theabrownines (TBs) and TRs/TFs ratio in converted tea brews under two-stage enzyme catalyzed fermentation and extraction conditions were further compared between different treatments, based on which the optimised conditions were determined. …”
Get full text

Subtilisin-like protease 4 regulates cold tolerance through cell wall modification in rice by Jingyan Liu, Fei He, Zhicai Chen, Meng Liu, Yingni Xiao, Ying Wang, YuMeng Cai, Jin Du, Weiwei Jin, Xuejun Liu

“…Plant subtilisin-like proteases (SUBs or SBTs) are protein-hydrolyzing enzymes which play important roles in various aspects of plant growth as well as the plant response to biotic and abiotic stress. …”
Get full text

Synthesis and Physico-Chemical Analysis of Dextran from Maltodextrin via pH Controlled Fermentation by <i>Gluconobacter oxydans</i> by Seung-Min Baek, Bo-Ram Park, Legesse Shiferaw Chewaka, Yun-Sang So, Ji-Hye Jung, Seul Lee, Ji Young Park

“…This study examined how jar fermentor conditions impact the growth and enzyme activity of <i>G. oxydans</i>, with a focus on the effects of pH on dextran synthesis via bioconversion (without pH control, pH 4.5, and pH 5.0; Jp-UC, Jp-4.5, and Jp-5.0). …”
Get full text

Do all patients with rheumatic Diseases have a higher risk of COVID 19? Initial results from the Karnataka Rheumatology Association COVID 19 Cohort Study (KRACC) by Vineeta Shobha, K Chanakya, Vikram Haridas, Sharath Kumar, Pramod Chebbi, Benzeeta Pinto, Vikramraj Jain, Subramaniam Ramaswamy, Shiv Prasad, Abhishek Patil, Vijay K Rao, G C Yathish, B G Dharmanand, Ramesh Jois, Ashwini Kamath, Chethana Dharmapalaiah, K N Sangeeta, Ramya Janardana, C Srinivasa, A S Harshini, Nagaraj Srinivasulu, Yogesh Preet Singh, Shweta Singhai, K M Mahendranath, S Chandrashekara

“…Age >60 years (P = 0.01), diabetes (P = 0.009), hypertension (P = 0.001), preexisting lung disease (P = 0.0002), current prescription of either angiotensin-converting enzyme inhibitor or angiotensin receptor blockers (P = 0.01), and higher glucocorticoids dosage (P = 0.002) were identified as potential risk factors in our cohort. …”
Get full text