Harnessing the synergy of Urochloa brizantha and Amazonian Dark Earth microbiomes for enhanced pasture recovery by Anderson Santos de Freitas, Luís Felipe Guandalin Zagatto, Gabriel Silvestre Rocha, Franciele Muchalak, Guilherme Lucio Martins, Solange dos Santos Silva-Zagatto, Rogério Eiji Hanada, Aleksander Westphal Muniz, Siu Mui Tsai

“…The combined influence of U. brizantha roots and ADEs exhibited synergies that enhanced prokaryotic diversity and enzyme activity. This balance supported plant growth and increased the general availability of beneficial bacteria in the soil, such as Chujaibacter and Curtobacterium while reducing the presence of potentially pathogenic taxa. …”
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Optimization of Starch Saccharification Process and Structural Characterization of Starch Using Low Transglycosidation Activity Glucoamylase GluM3 by Haiyue FAN, Zhao YU, Zhengjie ZHANG, Ming LI, Yingguo BAI, Feng YU, Zhongyuan LI

“…The results showed that the optimal saccharification conditions were a liquefaction DE value of 16%, temperature at 66 ℃, pH at 3.7, time at 3 h, enzyme dosage at 450 U/g with pullulanase dosage at 1.25 U/g. …”
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Evaluation of Cottonseed Meal as an Alternative to Fish Meal in Diet for Juvenile Asian Red-Tailed Catfish Hemibagrus wyckioides by Biwei Li, Linhai Su, Yan Sun, Han Huang, Junming Deng, Zhiyong Cao

“…These results indicated that dietary CSM inclusion level up to 17.2% improved the growth rate, feed cost, digestive enzyme activity, and protein metabolism without compromising antioxidant capacity of H. wyckioide, whereas these parameters were depressed by further inclusion of CSM. …”
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Nationality, Gender, Age, and Body Mass Index Influences on Vitamin D Concentration among Elderly Patients and Young Iraqi and Jordanian in Jordan by Hanan Al-Horani, Wael Abu Dayyih, Eyad Mallah, Mohammed Hamad, Mohammad Mima, Riad Awad, Tawfiq Arafat

“…Vitamin D levels were evaluated using enzyme-linked immunosorbent assay. For young healthy group subjects, vitamin D levels were 20.60 ± 5.94 ng/mL for Jordanian and 27.59 ± 7.74 ng/mL for Iraqi. …”
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Computational Analysis of nsSNPs of ADA Gene in Severe Combined Immunodeficiency Using Molecular Modeling and Dynamics Simulation by Soukaina Essadssi, Al Mehdi Krami, Lamiae Elkhattabi, Zouhair Elkarhat, Ghita Amalou, Houria Abdelghaffar, Hassan Rouba, Abdelhamid Barakat

“…The ADA gene encodes adenosine deaminase, an enzyme that catalyzes the irreversible deamination of adenosine and deoxyadenosine in the catabolic pathway of purine. …”
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CHST1 as a prognostic-related biomarker in gastric cancer correlating with immune infiltration by Long Gui, Jin-hao Yu, Jia-nan Tan, Guang-yu Zhong, Yong-xue Wang, Lin Zhong, Jiao-jiao Su, Dong Hou, Bin Yang, Fang-hai Han, Sheng-ning Zhou

“…Carbohydrate sulfotransferase (CHST) is a Golgi membrane-associated enzyme with potential as a cancer diagnostic marker. …”
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An in-vivo herb-drug interaction study of IME-9 and glimepiride in nicotinamide-STZ-induced diabetic rats by Archana K. Thikekar, Asha B. Thomas, Sohan S. Chitlange

“…In silico molecular docking investigations were conducted using Autodock Vina 1.2.3 to assess the binding affinity of the 46 primary phytoconstituents present in IME 9 with CYP2C9 enzymes. Toxicity assessments were carried out utilizing the MTT assay. …”
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Secretory phospholipase A2 inhibitory activity in Tragia hispida: A novel and promising therapeutic agent for dengue fever by D.V. Dayangi Hemalika, U.G. Chandrika, Ajita M. Abeysekera, Sameera R. Samarakoon, Ananda Wijewickrama, Gathsaurie Neelika Malavige

“…Secretory phospholipase A2 (sPLA2), an inflammatory enzyme, plays a critical role in producing platelet-activating factor (PAF), a key mediator of vascular leak. …”
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Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal:... by S. Gautam, N. R. Bhattarai, K. Rai, A. Poudyal, B. Khanal

“…Carbapenem resistance among Gram-negative isolates caused by the production of the metallo-β-lactamase (MBL) enzyme is being increasingly reported worldwide. One of the newly emerged metallo-β-lactamases is New Delhi metallo-β-lactamase. …”
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Decursinol angelate relieves inflammatory bowel disease by inhibiting the ROS/TXNIP/NLRP3 pathway and pyroptosis by Yudi Wang, Yudi Wang, Jiamin Wang, Yonghu Chen, Xuezheng Li, Xuezheng Li, Zhe Jiang, Zhe Jiang

“…IntroductionDespite evidence of the efficacy of decursinol angelate (DA), a prescription medication derived farom traditional Chinese medicine, in alleviating inflammatory bowel disease (IBD), the precise mechanisms behind its action remain unclear.MethodsLipopolysaccharides (LPS) and dextran sodium sulfate (DSS) induction were used as in vitro and in vivo models of IBD, respectively, to assess the role of DA in alleviating IBD. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression levels of pro-inflammatory cytokines in mouse serum, Western blot was performed to detect the expression of TXNIP/NLRP3 pathway tight junction (TJ) proteins in colon tissues and cells, and immunohistochemistry, immunofluorescence and immunohistochemistry, immunofluorescence and qRT-PCR were used to validate the proteins related to this signaling pathway. …”
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Salivary Levels of Interleukin-17 in Iranian Patients With Systemic Sclerosis by Ali Tadayon, Javad Moayedi, Mohamad Ali Nazarinia, Mahyar Malekzadeh, Sara Kazemi, Maryam Mardani

“…The salivary levels of IL-17 in all samples were measured using a commercially available enzyme–linked immunosorbent assay (ELISA) kit. The mean salivary levels of IL-17 in patients with SSc were significantly higher than the control group (199.6±38.9 pg/mL vs. 112.7±39.4 pg/mL, P<0.0001). …”
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A meta-analysis on plant growth and heavy metals uptake with the application of 2,4-epibrassinolide in contaminated soils by Kuiju Niu, Hong Xiao, Yong Wang, Ting Cui, Chunxu Zhao

“…EBR application enhanced photosynthesis and the mitigation of oxidative damage by significantly boosting antioxidant enzyme activity, non-enzymatic antioxidants, and metabolites. …”
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The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition by Dongfang Zhao, Bingqian Zhou, Bo Hong, Jiajun Mao, Hu Chen, Junjie Wu, Li Liao, Chunyun Guan, Mei Guan

“…Fatty acids play various roles in plants, but the functions of the genes involved in the fatty acid composition during plant development remain unclear. β-Ketoacyl-CoA synthase (KCS) is a key enzyme involved in the elongation of fatty acids. …”
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A Case of Concurrent MPO-/PR3-Negative ANCA-Associated Glomerulonephritis and Membranous Glomerulopathy by Yasuyuki Nakada, Nobuo Tsuboi, Yasuto Takahashi, Hiraku Yoshida, Yoriko Hara, Hideo Okonogi, Tetsuya Kawamura, Yoshihiro Arimura, Takashi Yokoo

“…Indirect immunofluorescence examination of the patient’s neutrophils showed a perinuclear pattern. Enzyme-linked immunosorbent assays revealed that the ANCA in this case did not target myeloperoxidase (MPO) or proteinase 3 (PR3) but bactericidal-/permeability-increasing protein, elastase, and lysosome. …”
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A thorny pathway of macrophage activating factor (GcMAF): from bench to bedside by A. A. Ostanin, S. S. Kirikovich, E. V. Dolgova, А. S. Proskurina, E. R. Chernykh, S. S. Bogachev

“…Besides its anti-tumor activity, GcMAF exerts a potential against a number of viral and neurodegenerative diseases associated with increased activity of N-acetylgalactosaminidase (nagalase) in the blood serum of patients. Nagalase is an enzyme that completely (rather than selectively) deglycosylates DBP so it cannot be converted to GcMAF, leading to immunodeficiency. …”
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Genomic identification, evolutionary analysis, and transcript profiling of protein phosphatase 2C in Solanum lycopersicum by Miah Mohammad Sakib, Md. Sifatul Islam, Asifur Rob Bhuya, Md. Rihan Kabir Shuvo, Mohammad Abdullah-Al-Shoeb, Muhammad Abul Kalam Azad, Ajit Ghosh

“…Abstract Protein phosphatases (PPs) are a class of enzymes that play a critical role in cellular regulation by catalyzing the removal of phosphate groups from proteins. …”
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Tear neuropeptides are associated with clinical symptoms and signs of dry eye patients by Yirou Zhang, Hong Zhang, Xingyu Zhu, Han Ye, Kan Yang, Xujiao Zhou, Jiaxu Hong

“…Tear fluid samples were collected and enzyme-linked immunoassay (ELISA was performed to analyze levels of calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and substance P (SP), as well as the association among the 3 neuropeptides and clinical findings.Results Compared to normal controls, levels of tear CGRP (p=.003) and SP (p=.002) were significantly decreased in dry eye patients yet not NPY concentrations. …”
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Relationship between Serum Cytokeratin-18, Control Attenuation Parameter, NAFLD Fibrosis Score, and Liver Steatosis in Nonalcoholic Fatty Liver Disease by Sumitro Kosasih, Wong Zhi Qin, Rafiz Abdul Rani, Nazefah Abd Hamid, Ngiu Chai Soon, Shamsul Azhar Shah, Yazmin Yaakob, Raja Affendi Raja Ali

“…Patients who underwent abdominal US were recruited, followed with measurement of CAP using Fibroscan® and serum fragmented CK-18 using enzyme-linked immunosorbent assay. The degree of liver steatosis assessed by US was categorized into mild (S1), moderate (S2), and severe (S3). …”
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Relieving Effect of <i>Artemisia ordosica Krasch</i> Extract on DSS-Induced Colitis by Regulating Immunity, Antioxidant Function, Gut Microbiota, and Bile Acid Metabolism in Mice by Min Jiang, Xuekai Zhang, Xiao Jin, Binlin Shi, Yuanqing Xu, Zheqi Wang

“…In vivo studies demonstrated that AOE reduced DSS-induced colitis in mice by increasing the colon length, enhancing antioxidant enzyme activity, inhibiting inflammatory cell infiltration, suppressing the formation of TNF-α and IL-6, and reducing malondialdehyde (MDA) levels. qPCR analysis revealed that AOE reversed the down-regulation of <i>Claudin</i> mRNA expression, and altered the composition of cecal microbiota, thus mitigating DSS-induced colitis. …”
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A Cross-Sectional Trait Versus State Biomarker Analysis of Inflammatory Cytokines and miRNAs in Patients with Major Depressive Disorder by Christopher Rockson, Chandrashekaran Girish, Harivenkatesh Natarajan, Vikas Menon

“…Serum levels of IL-6, IL-1β, TNF-α, hs-CRP, and IL-10 were evaluated using the Enzyme-Linked Immunosorbent Assay (ELISA) method. …”
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