Levo-Tetrahydropalmatine Attenuates Bone Cancer Pain by Inhibiting Microglial Cells Activation by Mao-yin Zhang, Yue-peng Liu, Lian-yi Zhang, Dong-mei Yue, Dun-yi Qi, Gong-jian Liu, Su Liu

“…The concentrations of TNF-α, IL-1β, and IL-18 in spinal cord were measured by enzyme-linked immunosorbent assay. Western blot was used to test the activation of astrocytes and microglial cells in spinal cord after TCI treatment. …”
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Cell–cell heterogeneity in phosphoenolpyruvate carboxylase biases early cell fate priming in Dictyostelium discoideum by Kenichi Abe, Hidenori Hashimura, Haruka Hiraoka, Shoko Fujishiro, Narufumi Kameya, Kazuteru Taoka, Satoshi Kuwana, Masashi Fukuzawa, Satoshi Sawai, Satoshi Sawai, Satoshi Sawai

“…Our results suggest the importance of cell–cell heterogeneity in the levels of metabolic enzymes during early cell fate priming.…”
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Positive feedback loop involving AMPK and CLYBL acetylation links metabolic rewiring and inflammatory responses by Wenke Wang, Boquan Wu, Mingjun Hao, Sichong Chen, Ruiting Cong, Wenjie Wu, Pengbo Wang, Qiaoyi Zhang, Pengyu Jia, Yuequn Song, Bo Liu, Siyao Qu, Jian-Fei Pei, Da Li, Naijin Zhang

“…Mechanistically, we found a crucial AMPK-CLYBL acetylation positive feedback loop, triggered by toll-like receptors (TLRs), involving AMPK hypophosphorylation and CLYBL hyperacetylation. The deacetylase enzyme SIRT2 acted as the bridge between AMPK phosphorylation and CLYBL acetylation, thereby regulating macrophage polarization and the release of pro-inflammatory cytokines. …”
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Investigating the Effect of Testosterone Supplement on Heart and Muscle Damage in Rats Applied with Swimming Exercise by Ömer Kulaksız, Sefa Lök

“…When between-groups values of troponin I, ck-MB, and LDH, which are indicators of heart damage, and enzyme CK, which is an indicator of muscle damage,for control, exercise, testosterone, and testosterone+exercise groups were compared, a statistically significant difference was determined between control, exercise, testosterone, and testosterone+exercise groups (p lt;0.05). …”
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Exploring Echinacea angustifolia for anti-viral compounds against Zika virus RNA-dependent RNA polymerase: a computational study by Mai M. El-Daly, Leena H. Bajrai, Thamir A. Alandijany, Isra M. Alsaady, Hattan S. Gattan, Meshari M. Alhamdan, Vivek Dhar Dwivedi, Esam I. Azhar

“…This study explores the potential of compounds from Echinacea angustifolia (E. angustifolia) to inhibit the activity of ZIKV’s RNA-dependent RNA polymerase (RDRP), a key enzyme in the viral replication process and an ideal candidate for antiviral therapy. …”
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Methylome profiling of cell-free DNA during the early life course in (un)complicated pregnancies using MeD-seq: Protocol for a cohort study embedded in the prospective Rotterdam pe... by Marjolein M van Vliet, Sam Schoenmakers, Ruben G Boers, Lotte E van der Meeren, Joost Gribnau, Régine P M Steegers-Theunissen

“…A methylation-dependent restriction enzyme (LpnPI) will be used to generate DNA fragments followed by sequencing on the Illumina NextSeq2000 platform. …”
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Correlation of serum Vitamin D levels and disease severity in patients with atopic dermatitis: A hospital-based, cross-sectional study from North India by Shreya Malhan, Loveleen Kaur, Maninder Kaur, Dimple Chopra, Sharang Gupta, Snehdeep Kaur

“…Disease severity was determined according to the SCORing Atopic Dermatitis (SCORAD) index, and Vitamin D levels were estimated by performing an enzyme-linked immunosorbent assay technique on the blood sample taken from the study participants. …”
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Molecular Mechanism of Macrophage Activation by Red Ginseng Acidic Polysaccharide from Korean Red Ginseng by Se Eun Byeon, Jaehwi Lee, Ji Hye Kim, Woo Seok Yang, Yi-Seong Kwak, Sun Young Kim, Eui Su Choung, Man Hee Rhee, Jae Youl Cho

“…According to pharmacological evaluation with specific enzyme inhibitors, Western blot analysis of intracellular signaling proteins and inhibitory pattern using blocking antibodies, ERK, and JNK were found to be the most important signaling enzymes compared to LPS signaling cascade. …”
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Perbedaan Kadar Hepsidin pada Remaja Putri Anemia dan Non Anemia by Putri Engla Pasalina, Nirmala Sari, Hendri Devita

“…Kadar hepsidin merupakan kadar yang diperoleh dari hasil pengukuran serum hepcidin-25 responden yang diukur dengan metode Enzyme-Linked Immunosorbent Assays (ELISA). Uji statistik yang digunakan adalah uji Mann Whitney karena data tidak berdistribusi normal. …”
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A Korean Patient With Leber Congenital Amaurosis and a Homozygous RPE65 Variant Originating From a Paternal Uniparental Isodisomy by Hane Lee, Dongseok Moon, Rin Khang, Go Hun Seo, Chang Ki Yoon, Un Chul Park, Kyu Hyung Park, Eun Kyoung Lee

“…RPE65, one of the causative genes for LCA, encodes retinoid isomerohydrolase, an enzyme that plays a critical role in regenerating visual pigment in photoreceptor cells. …”
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Clinicopathological characteristics and gene mutations in 11 patients with lipoprotein glomerulopathy by Yan Qin, Xiao-Jing Sun, Yi-Fang Hu, Meng Jing, Xiao-Juan Yu, Ming-Hui Zhao, Ying Tan

“…Following the confirmation of LPG diagnosis, treatment with angiotensin-converting enzyme inhibitor (ACEI)/angiotensin receptor blocker (ARB) was initiated, and the disease progressed slowly.Conclusion LPG is histologically characterized by lamellated lipoprotein thrombi in glomeruli, and kidney biopsy is essential for diagnosis. …”
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Synthesis, Molecular Docking, MEP and SAR Analysis, ADME-Tox Predictions, and Antimicrobial Evaluation of Novel Mono- and Tetra-Alkylated Pyrazole and Triazole Ligands by Y. Kaddouri, B. Bouchal, F. Abrigach, M. El Kodadi, M. Bellaoui, R. Touzani

“…Of particular interest, these different MEPs were evident in ligand protein docking, suggesting that compound 12 has better affinity with MGL enzyme than compound 13. All these findings suggested that these novel compounds represent promising antibacterial lead compounds.…”
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Optical control of sphingolipid biosynthesis using photoswitchable sphingosines by Matthijs Kol, Alexander J.E. Novak, Johannes Morstein, Christian Schröer, Tolulope Sokoya, Svenja Mensing, Sergei M. Korneev, Dirk Trauner, Joost C.M. Holthuis

“…Sphingolipid metabolism comprises a complex interconnected web of enzymes, metabolites, and modes of regulation that influence a wide range of cellular and physiological processes. …”
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Central Interleukin-1β Suppresses the Nocturnal Secretion of Melatonin by A. P. Herman, J. Bochenek, K. Król, A. Krawczyńska, H. Antushevich, B. Pawlina, A. Herman, K. Romanowicz, D. Tomaszewska-Zaremba

“…IL-1β also inhibited (P<0.05) the expression of the melatonin rhythm enzyme arylalkylamine-N-acetyltransferase and hydroxyindole-O-methyltransferase. …”
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Effects of IQW and IRW on Inflammation and Gut Microbiota in ETEC-Induced Diarrhea by Naiyuan Liu, Lingsi Zhou, Jun Fang, Hongmei Jiang, Gang Liu

“…., TNF-α, IL-1β, IL-6, and IL-10) using the enzyme-linked immunosorbent assay (ELISA). The microbiota in the colon was sequenced using 16S ribosomal RNA. …”
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Circ-PDE1C/miR-766-3p/SGTB axis regulates the IL-1β-induced apoptosis, inflammation and oxidative stress in human chondrocytes by Lixia Gao, Tao He, Qingkui Hu, Yan Ma

“…The inflammatory cytokines were measured by Enzyme-linked immunosorbent assay (ELISA). Oxidative stress was assessed by commercial kits. …”
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BvBZR1 improves parenchyma cell development and sucrose accumulation in sugar beet (Beta vulgaris L.) taproot by Ningning Li, Wei Wang, Wei Wang, Xiaotong Guo, Yaqing Sun, Guolong Li, Shaoying Zhang

“…These results suggest that BvBZR1 can regulate the expression of genes related to cell wall and sucrose metabolism, improve corresponding enzyme activity, and promote the development of each cambium ring and parenchyma cell, thereby promoting the growth and development of sugar beet taproots.…”
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Assessment of liver fibrosis by acoustic radiation force impulse elastography in rheumatic disease patients on long-term methotrexate treatment by Arindam Nandy Roy, Gayatri Senapathy, Vijay Kumar Bongu, Yarram Ashok Kumar, Syeda Sana Fatima, Regina Lata Thadigiri

“…Liver fibrosis is rare in rheumatoid arthritis patients treated with a high cumulative dose of methotrexate, and is not accurately detected in patients with liver enzyme abnormalities. Conclusion: Considering the risk and benefit ratio of liver biopsy, ARFI may be a useful non- invasive diagnostic tool for the monitoring hepatic toxicity in patients with rheumatic disease undergoing methotrexate treatment.…”
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Complement Factor H Gene Variant in a Patient with Thrombotic Microangiopathy on a Mixed Clinical Background by Yoichi Iwafuchi, Tetsuo Morioka, Yuko Oyama, Shin Goto, Ichiei Narita

“…She was able to withdraw from hemodialysis therapy two weeks after the initiation of an angiotensin-converting enzyme inhibitor. Based on her clinical course and kidney biopsy findings, she was diagnosed with thrombotic microangiopathy with a very rare CFH variant. …”
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A case report: interstitial pneumonia following treatment of gastric cancer with sintilimab in combination with S-1 by Pei Zhu, Qingming Sun, Sheng Xu, Wanhui Dong

“…The treatment was discontinued, and after receiving adequate glucocorticoid therapy, the pulmonary lesions showed slight improvement.ConclusionThis case provides a clinical reference, indicating that prior touse of sintilimab in combination with S-1 antitumor regimen, a comprehensive baseline assessment should be conducted, including blood routine examination, enzyme tests, and pulmonary imaging examination, with close monitoring of the patient’s pulmonary condition. …”
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