Microbial phenolic metabolites 3-(3′,4′-dihydroxyphenyl)propanoic acid and 3′,4′-dihydroxyphenylacetic acid prevent obesity in mice fed with high-fat diet by Wanbing Chen, Ruonan Liu, Xiaoling Zhu, Qun Lu, Hong Yang, Rui Liu

“…Correlation analysis revealed that the differential metabolites are strongly associated with body weight, blood glucose, insulin level, and superoxide dismutase (SOD) enzyme activity. Our results revealed that DHPA and DHAA exert their anti-obesity effect by regulating important metabolites in the glucose, lipid and tyrosine metabolism pathways.…”
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CADD-based discovery of novel oligomeric modulators of PKM2 with antitumor activity in aggressive human glioblastoma models by Maia Cabrera, Romina Armando, Ian Czarnowski, Patricio Chinestrad, Ramiro Blanco, Alejandra Zinni, Daniel Gómez, Diego L. Mengual Gómez, Pablo Lorenzano Menna

“…Pyruvate kinase isoform M2 (PKM2) is a multifunctional enzyme capable of transitioning between monomeric, dimeric, and tetrameric states, with its oligomeric equilibrium playing a pivotal role in tumour progression and survival. …”
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Circulating Total and Active Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinases-1 in Patients with Systemic Lupus Erythomatosus

“…Serum levels of tMMP-9 and TIMP-1 were assessed by an enzyme-linked immunosorbent assay (ELISA) and aMMP-9 by fluorometric assay. …”
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Anti-proteolytic regulation of KRAS by USP9X/NDRG3 in KRAS-driven cancer development by Han Koo, Kyung Chan Park, Hyun Ahm Sohn, Minho Kang, Dong Joon Kim, Zee-Yong Park, Sehoon Park, Sang Hyun Min, Seong-Hwan Park, Yeon-Mi You, Yohan Han, Bo-Kyung Kim, Chul-Ho Lee, Yeon-Soo Kim, Sang J. Chung, Young Il Yeom, Dong Chul Lee

“…Here, we report that KRAS expression is post-translationally up-regulated through deubiquitination when the scaffolding function of NDRG3 (N-Myc downstream-regulated gene 3) promotes specific interaction between KRAS and a deubiquitinating enzyme, USP9X. In KRAS-mutant cancer cells KRAS protein expression, downstream signaling, and cell growth are highly dependent on NDRG3. …”
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Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig by Kunhong Xie, Weidong Cai, Lingjie Li, Bing Yu, Yuheng Luo, Zhiqing Huang, Xiangbing Mao, Jie Yu, Ping Zheng, Hui Yan, Hua Li, Jun He

“…The decrease in intestinal villus height, villous height: crypt depth ratio and digestibility was accompanied by lower expression of ZO1, ZO2, Claudin1, DMT1, CAT1, SGLT1 and PBD114 genes, as well as decreased enzyme activities of intestinal alkaline phosphatase, lactase, sucrase and maltase of piglets in DA group compared to piglets in DCON group. …”
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Thyroid Hormone Activation Regulates the Crosstalk between Breast Cancer and Mesenchymal Stem Cells by Annarita Nappi, Vittoria D’Esposito, Caterina Miro, Alessia Parascandolo, Annunziata Gaetana Cicatiello, Serena Sagliocchi, Lucia Acampora, Sepehr Torabinejad, Federica Restolfer, Maddalena Raia, Melania Murolo, Emery Di Cicco, Pietro Formisano, Monica Dentice

“…MCF7 cells enhanced the intracellular activation of THs through the TH-activating enzyme, D2, fostering their EMT properties and the dialogue with MSCs. …”
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Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos by Yuxin Chen, Ziwen Yang, Zhiqiang Guo, Lemei Zhan, Kenneth B. Storey, Danna Yu, Jiayong Zhang

“…CPF exhibits its toxicity primarily through the inhibition of the acetylcholinesterase (AChE) enzyme, while mitochondrial damage and dysfunction have also been observed. …”
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Systemic delivery of AAV5, AAV8, and AAV9 packaging a C5-12-microdystrophin-FLAG expression cassette in non-human primates by Mengping Liu, Erica Cook, Yanshan Dai, Erich Ehlert, Francois du Plessis, Jacek Lubelski, Bogdan G. Sleczka, Petia Shipkova, Zhuyin Li, Joshua Gamse, David Gordon, Leonard P. Adam, Paul C. Levesque, Glen B. Banks

“…At 1 month post-dose, we compared tissue vector genomes, mRNA, and microdystrophin-FLAG protein levels by meso-scale discovery-enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry, and immunofluorescence. …”
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A novel nomogram to predict all‐cause readmission or death risk in Chinese elderly patients with heart failure by Mengxi Yang, Liyuan Tao, Hui An, Gang Liu, Qiang Tu, Hu Zhang, Li Qin, Zhu Xiao, Yu Wang, Jiaxai Fan, Dongping Feng, Yan Liang, Jingyi Ren

“…Anaemia, abnormal neutrophils, and admission without angiotensin‐converting enzyme inhibitors/angiotensin receptor blockers were the specific independent risk factors of 30 day all‐cause readmission or death (all P < 0.05), whereas serum sodium ≤ 140 mmol/L and admission without beta‐blockers were the specific independent risk factors of 1 year all‐cause readmission or death (all P < 0.05). …”
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Multigenerational effects of cultivating transgenic maize straw on earthworms: A combined laboratory and field experiment by Li Zhang, Wenjing Shen, Zhixiang Fang, Laipan Liu, Ruizong Jia, Jingang Liang, Biao Liu

“…The enzymatic activity assay results revealed no significant differences in superoxide dismutase (SOD) or catalase (CAT) enzyme activity between the GM and non-GM maize varieties. …”
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Nutritional Innovation Using Green Seaweed (Ulva sp.) and Garlic Powder Extracts for White‐Leg Shrimp (Litopenaeus vannamei) Challenged by Vibrio harveyi by Nashwa Abdel‐Razek, Riad H. Khalil, Abeer A. M. Afifi, Afrah F. Alkhuriji, Dina M. Metwally

“…There were significant increases in serum total protein, acid phosphatase activity, alkaline phosphatase, lysosomal enzyme activity, phenoloxidase activity and superoxide dismutase activity with offered diets with increasing the levels of ethanolic extracts of Ulva sp. and garlic powder up to 2.0 g UE/kg diet and 6 g GPE/kg diet, respectively. …”
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Tryptanthrin exerts anti-breast cancer effects both in vitro and in vivo through modulating the inflammatory tumor microenvironment by Zeng Qingfang, Luo Cairong, Cho Junlae, Lai Donna, Shen Xiangchun, Zhang Xiaoyan, Zhou Wei

“…After two weeks of tryptanthrin treatment, samples from serum, tumor tissue and different organs from tumor-bearing mice were collected, and the enzyme-linked immunosorbent assay (ELISA) was performed to assess the regulation of inflammatory molecules in mouse serum. …”
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Screening of West Siberian patients with primary congenital glaucoma for CYP1B1 gene mutations by D. E. Ivanoshchuk, S. V. Mikhailova, O. G. Fenkova, E. V. Shakhtshneider, A. Z. Fursova, I. V. Bychkov, M. I. Voevoda

“…Mutations in the CYP1B1 gene, which encodes cytochrome P450 1B1, are the most common cause of autosomal recessive primary congenital glaucoma. This enzyme is known to be involved in retinoic acid metabolism and is necessary for normal eye development. …”
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Multi-omics reveals that ST6GAL1 promotes colorectal cancer progression through LGALS3BP sialylation by Yuanchao Shi, Zhenzhong Pan, Jingwei Duan, Zexing Wang, Yiliang Fang, Bo Tang, Quanlin Guan

“… ST6 β-galactoside α2,6-sialyltransferase 1 (ST6GAL1), a crucial enzyme for tumor-associated sialic acid modification, has been reported to positively correlate with colorectal cancer (CRC) tumorigenesis; however, the underlying mechanism remains unclear. …”
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Is Amanita phalloides Nephrotoxicity due to Mitochondrial Toxicity? by Jules Weinhard, Justine Serre, Perrine Frère, Clovis Adam, Marie Camille Lafargue, David Buob, Cédric Rafat

“…However, one key mitochondrial function, involving an enzyme called complex IV, appeared to be unaffected.This case suggests that the death cap mushroom may harm kidneys by damaging mitochondria in a way that leaves certain functions intact.…”
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5,10-methylenetetrahydrofolate reductase C677T gene polymorphism as a risk factor for premature coronary artery disease in patients with type 2 diabetes mellitus by Nisreen O. Mohammed, Ibtisam A. Ali, Bahaelddin K. Elamin, Bakri Osman Saeed

“…Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP), using Hinf1 restriction enzyme, were used to determine MTHFR genotypes. Plasma homocysteine levels were determined by enzymatic assay on the Hitachi Cobas Integra® 400 plus. …”
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Micro-/nanobubble oxygenation irrigation enhances soil phosphorus availability and yield by altering soil bacterial community abundance and core microbial populations by Qingyong Bian, Qingyong Bian, Zhiduo Dong, Yupeng Zhao, Yaozu Feng, Yaozu Feng, Yaozu Feng, Yanbo Fu, Yanbo Fu, Zhiguo Wang, Jingquan Zhu, Liang Ma

“…Therefore, we established two irrigation methods, conventional irrigation (CF) and micro-/nanobubble oxygenation irrigation (MB), to investigate the combined effects on enzyme activity, microbial communities, and soil phosphorus availability in the rhizosphere soil of maize.The results showed that compared to the CF treatment, the MB treatment significantly increased available phosphorus content and alkaline phosphatase activity in maize rhizosphere soil by 21.3% and 15.4%, respectively. …”
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Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis by Marielle Garcia Silva, Juliana Santana de Curcio, Mirelle Garcia Silva-Bailão, Raisa Melo Lima, Mariana Vieira Tomazett, Aparecido Ferreira de Souza, Vanessa Rafaela Milhomem Cruz-Leite, Nicolau Sbaraini, Alexandre Melo Bailão, Fernando Rodrigues, Maristela Pereira, Relber Aguiar Gonçales, Célia Maria de Almeida Soares

“…Paracoccidioides spp. present orthologous genes encoding the enzymes necessary for the biosynthesis of hydroxamates, and plasma membrane proteins related to the transport of these molecules. …”
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Targeting HVEM-GPT2 axis: a novel approach to T cell activation and metabolic reprogramming in non-small cell lung cancer therapy by Yuanshan Yao, Chunji Chen, Bin Li, Wen Gao

“…RNA sequencing illuminated a potential relationship between HVEM and GPT2, an enzyme tied to amino acid metabolism and cellular energetics. …”
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Effect of acid whey protein concentrate on the rheological properties, antioxidant capacities, and biological activities of bioaccessible fractions in fermented camel milk by Mutamed Ayyash, Abdelmoneim H. Ali, Anas Al-Nabulsi, Tareq Osaili, Gafar Bamigbade, Athira Subash, Basim Abu-Jdayil, Afaf Kamal-Eldin

“…The biological activities, including α-amylase, α-glucosidase, and angiotensin-converting enzyme inhibition, increased after in vitro digestion of yogurt samples. …”
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