Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord by Ryan J. Emnett, Aparna Kaul, Aleksandar Babic, Vicki Geiler, Donna Regan, Gilad Gross, Salem Akel

“…To support the development of a cGMP-compliant method, we have examined an enzyme-free isolation method utilizing tissue homogenization (t-H) followed by culture in human platelet lysate (PL) supplemented media. …”
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The Serum S100B Level as a Biomarker of Enteroglial Activation in Patients with Ulcerative Colitis by Asuman Celikbilek, Mehmet Celikbilek, Seda Sabah, Nermin Tanık, Elif Borekci, Serkan Dogan, Yavuz Akin, Suleyman Baldane, Kemal Deniz, Neziha Yilmaz, Omer Ozbakir, Mehmet Yucesoy

“…Serum samples were analyzed for human GFAP and S100B using commercial enzyme-linked immunosorbent assay kits. Results. GFAP was not detected in the serum of either UC patients or controls (P>0.05). …”
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The Potential of Defatted Yellow Mealworm (Tenebrio molitor) Meal as an Alternative Protein Source for Juvenile Chinese Mitten Crab (Eriocheir sinensis) by Wenxiang Yao, Chunyan Zhang, Sai Zhang, Guoan Hua, Sitong Zhao, Huiyang Shuang, Ningyang Sun, Yijun Sun, Lumpan Poolsawat, Jianguo Wang, Quan Wang

“…The results showed that the growth, serum immune and antioxidant indexes, digestive enzyme activities, intestinal histology, and microbiota composition of DYM25, DYM50, and DYM75 groups all reached the similar level as the CON group. …”
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Influence of multimodal low-opioid anesthesia on the dynamics of аnnexin V in blood plasma during cardiac surgery by S.R. Maruniak, O.A. Loskutov, O.M. Druzhyna, I.R. Malish, N.V. Korotchuk

“…The determination of the level of аnnexin V in the blood was carried out before CPB and after bringing the sternum by enzyme-linked immunosorbent assay. Results. The low-opioid scheme of anesthetic management was associated with significantly (by 1.5 times) lower level of аnnexin V and by 28.38 % lower level of interleukin-6 at the end of the surgery as compared to the standard scheme. …”
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The pro-inflammatory cytokine Tumor Necrosis Factor alpha (TNF-α) in Diabetic Nephropathy by Alia KH Mahmoud, Muhammed Ahmed Abd El Moety, Amal Khalifa Ahmed, Samer A. El-Sawy

“…TNF-α level in the serum was measured in 25 individuals with diabetes, 25 individuals with diabetic nephropathy, and 25 healthy controls by enzyme-linked immun-osorbent assay (ELISA). Results: As compared to healthy controls, the diabetes and DN groups had significantly higher serum levels of TNF-α. …”
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Nephrotic Syndrome and Idiopathic Membranous Nephropathy Associated with Autosomal-Dominant Polycystic Kidney Disease by Ramón Peces, Jorge Martínez-Ara, Carlos Peces, Mariluz Picazo, Emilio Cuesta-López, Cristina Vega, Sebastián Azorín, Rafael Selgas

“…Treatment with chlorambucil 0.2 mg/kg daily, low-dose prednisone, plus an angiotensin-converting enzyme inhibitor (ACEI) and an angiotensin II receptor blocker (ARB) for 6 weeks resulted in partial remission of his nephrotic syndrome for a duration of 10 months. …”
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Frequency and progression of azotemia during acute and chronic treatment of congestive heart failure in cats by Sarah Rogg, Jonathan P. Mochel, Debosmita Kundu, Melissa A. Tropf, Allison K. Masters, Darcy B. Adin, Jessica L. Ward

“…Treatment with an angiotensin converting enzyme inhibitor was associated with fewer lifetime KI events (P = .02). …”
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Heterologous Expression and Functional Analysis of <i>Exiguobacterium</i> Algin Lyase Gene by <i>Pichia pastoris</i> by Hanwen Wu, Kai Hou, Yutong Jiang, Mingjian Luan, Yuxia Sun, Xi He, Xiangzhong Zhao

“…The activity of the recombinant enzyme in 5 L high-density fermentation reached 1306 U/mg protein, 3.9 times that of the original <i>Exiguobacterium</i> strain. …”
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Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke by Chengbo Tan, Hideo Shichinohe, Zifeng Wang, Shuji Hamauchi, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Tsuneo Ito, Kohsuke Kudo, Shigeru Takamoto, Kiyohiro Houkin

“…Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. …”
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Human Tyrosinase Displayed on the Surface of Chinese Hamster Ovary Cells for Ligand Fishing of Tyrosinase Inhibitors from Medicinal Plants by Xiao-Rui Zhai, Ming-Jie Li, Xiang Yin, Ayzohra Ablat, Yuan Wang, Peng Shu, Xun Liao

“…The localization of hTYR on the CHO cell surface was verified by an enzyme activity test and fluorescence microscopy. …”
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Counteracting negative effects of salinity on Lepidium sativum L. seedlings by prepared biochar by Amira Mohamed Abd El-Sattar, Zeinab Ashour Shedeed

“…The use of prepared biochar at 0.5 and 1% reduced the damaging effects of salt stress by enhancing the activity of the α-amylase enzyme, resulting in a significant rise in germination (95% at 5000 mg/L by 0.5% of biochar). …”
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Eating Habits, Anxiety and Depression in Patients with Irritable Bowel Syndrome: Clinical and Laboratory Comparisons by O. V. Gaus, M. A. Livzan

“…All individuals included in the study were assessed for diet and eating habits using the WHO CINDI program questionnaire, “Information on Nutrition and Eating Behavior”, the severity of anxiety and depression according to the HADS questionnaire, the level of specific anxiety in relation to gastrointestinal symptoms according to the VSI questionnaire, quality of life according to the IBS-QoL questionnaire. In addition, the enzyme immunoassay method was used to assess the levels of cortisol in the morning and evening portions of saliva, serotonin in the blood serum and dopamine in the blood plasma.Results. …”
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Molecular and serological investigation of hepatitis B virus in patients with acute undifferentiated febrile illness at Tha Song Yang hospital, Tak Province, Thailand by Narin Thippornchai, Anon Sae-Oueng, Akanitt Jittmittraphap, Wang Nguitragool, Pornsawan Leaungwutiwong

“…The serum samples were subjected to enzyme-linked immunosorbent assay to detect antibodies against hepatitis B core antigen (HBcAg) and hepatitis B surface antigen (HBsAg). …”
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Exploring oral candidiasis among cancer patients undergoing chemotherapy in eastern Iran by Abdol Sattar Pagheh, Fatemeh Kardan, Aynaz Ghojoghi, Ahmad Reza Sebzari, Maryam Erfaninejad, Parvin Askari, Seyed Reza Aghili, Eisa Nazar, Masood Ziaee

“…Subsequently, Candida isolates were molecularly identified using the PCR-RFLP method with the restriction enzyme Msp1. Furthermore, the demographic characteristics, risk factors, and clinical history of the patients were extracted and scrutinized using a multiple logistic regression model. …”
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Fatal Renal Failure in a Spinal Cord Injury Patient with Vesicoureteric Reflux Who Underwent Repeated Ureteric Reimplantations Unsuccessfully: Treatment Should Focus on Abolition o... by Subramanian Vaidyanathan, Bakul Soni, Kottarathil Abraham Abraham, Peter Hughes, Gurpreet Singh

“…Angiotensin-converting enzyme inhibitors or angiotensin-receptor-blocking agents should be prescribed even in the absence of hypertension when a spinal cord injury patient develops vesicoureteral reflux and proteinuria.…”
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Discovery and Characterization of Two Selective Inhibitors for a Mu-Class Glutathione S-Transferase of 25 kDa from <i>Taenia solium</i> Using Computational and Bioinformatics Tools by César Sánchez-Juárez, Roberto Flores-López, Lluvia de Carolina Sánchez-Pérez, Ponciano García-Gutiérrez, Lucía Jiménez, Abraham Landa, Rafael A. Zubillaga

“…The two compounds were found to reduce the enzyme’s activity by 50–70% under assay conditions, while showing a reduction of only 30–35% for human mu-class GSTM1, demonstrating selectivity for Ts25GST. …”
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Progress Analysis and Development Prospects of Soil Ecological and Environmental Effects of Interlayer Construction by JIA Zhenjiang, WU Yangyang, LI Wangcheng, MA Dongxiang, CHEN Jihong, GAO Susu, NIU Xiaoxiao, XU Tianyuan

“…[Methods] The effects and regulation mechanisms of interlayer construction on soil physical structure, soil water-salt-heat, soil nutrient status, soil enzyme activities, soil microbial properties, and crop productivity were comprehensively addressed. …”
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Malyngamide F Possesses Anti-Inflammatory and Antinociceptive Activity in Rat Models of Inflammation by Zhuocheng Li, Lei Zhang, Zhichao Zhao

“…Levels of TNF-α, PGE-2, and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA). Results. …”
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Effects of Luteolin Treatment on Postharvest Quality and Antioxidant Capacity of Nanfeng Tangerines by Wenjuan Dong, Xiaohan Wang, Miaolian Xiang, Jinyin Chen, Jiaoke Zeng, Ming Chen

“…Furthermore, luteolin treatment inhibited malondialdehyde (MDA) and H₂O₂ accumulation by enhancing the content of total phenols and flavonoids content, augmenting antioxidant enzyme activities (peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD)), and elevating the overall antioxidant capacity measured through the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging rate. …”
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Korelasi Kadar Adiponektin dengan Indeks Aterogenik Plasma pada Penyandang Obes by Kartika Aulia Sari, Rismawati Yaswir, Tuty Prihandani, Efrida Efrida

“…Pemeriksaan kadar adiponektin dengan metode enzyme linked immunoassay (ELISA). Pemeriksaan kolesterol high density lipoprotein (HDL) dan trigliserida menggunakan alat kimia klinik otomatis. …”
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