Evaluating Methods for Aflatoxin B1 Monitoring in Selected Food Crops Within Decentralized Agricultural Systems by Haadia Tanveer, Hannah Glesener, Blake Su, Brooke Bolsinger, Rosa Krajmalnik-Brown, Lee E. Voth-Gaeddert

“…The objectives of this study were: (1) to evaluate common AFB1 detection methods, including enzyme-linked immunosorbent assays (ELISA) and lateral-flow assays (LFA), validated via high-performance liquid chromatography (HPLC), focusing on their suitability for possible applications in decentralized, low-resource settings; and (2) to conduct a barriers-to-use assessment for commonly available AFB1 detection methods and their applicability in low-resource settings. …”
Get full text

Scurvy as a Cause of Leg Pain and Limp in Pediatric and Adolescent Patients by Christopher J. DeFrancesco, MD, David Kell, BA, Sean Owens, BS, Matt Grady, MD, Naomi Brown, MD

“…Vitamin C deficiency (VCD) results in collagen dysfunction due to decreased lysyl and prolyl hydroxylase enzyme activity. The resulting disease, known as scurvy, can present with various findings, including gingival bleeding, bruising, other skin lesions, poor wound healing, and tooth and bone abnormalities. …”
Get full text

Transcriptional Regulation of Δ6-Desaturase by Peroxisome Proliferative-Activated Receptor δ Agonist in Human Pancreatic Cancer Cells: Role of MEK/ERK1/2 Pathway by Maryam Darabi, Shima Byagowi, Shabnam Fayezi, Masoud Darabi, Shahab Mirshahvaladi, Mehdi Sahmani

“…The Δ6-desaturase (Δ6D), also known as fatty acid desaturase 2, is a regulatory enzyme in de novo fatty acid synthesis, which has been linked to obesity and diabetes. …”
Get full text

Evaluation of Pentraxin-3 and Interleukin-6 Levels in Serum and Gingival Crevicular Fluid in Patients with Generalized Periodontitis and Periodontal Health Controls before and afte... by Siamak Yaghobee, Sadegh Hasannia, Fatemeh Hamidzadeh, Saman Valadan Tahbaz, Rojin Shahmohammadi, Farzaneh Poursafar

“…PTX3 and IL-6 levels in serum and GCF samples were evaluated by enzyme-linked immunosorbent assay. Data analysis was performed using Mann-Whitney U and Wilcoxon tests (P<0.05). …”
Get full text

Cloning and expression characterisation of LiDXS2 gene in Oriental Lily (Lilium 'Sorbonne') by Yu Jiaxuan, Wu Nana, Fu Ruyu, Xue Lili, Zhang Jinzhu, Dong Jie, Yang Tao, Fan Jinping

“…1-Deoxy-D-xylulose-5-phosphate synthase (DXS) is the first key enzyme in the MEP responsible for monoterpene synthesis. …”
Get full text

The immunohistochemical combination of low SGLT2 expression and high PRDX4 expression independently predicts shortened survival in patients undergoing surgical resection for hepato... by Yao Liu, Jia Han, Akihiro Shioya, Yang-Xian Zhang, Vu Anh Dung, Takeru Oyama, Xin Guo, Qian Yang, Tohru Ito, Sohsuke Yamada

“…Peroxiredoxin 4 (PRDX4) is an antioxidant enzyme with a secretory function and is located in the cytoplasmic endoplasmic reticulum. …”
Get full text

Synthesis, Characterization, and In Vivo Cytokinome Profile of IL-12-Loaded PLGA Nanospheres by Ryan A. Lacinski, Justin E. Markel, Jabeen Noore, Hillary G. Pratt, Brock A. Lindsey

“…Furthermore, elution of rmIL-12 from IL-12-NS at a concentration of 500 million particles/mL was characterized using enzyme-linked immunosorbent assay (ELISA). When IL-12-NS was administered in vivo to female BALB/c mice through retroorbital injection, IL-12-NS produced a favorable systemic cytokine profile for tumoricidal activity within the peripheral blood. …”
Get full text

Interleukin-10 in the Pathophysiology of Inflammatory Bowel Disease: Increased Serum Concentrations During the Recovery Phase by Keiichi Mitsuyama, Nobuo Tomiyasu, Kosuke Takaki, Junya Masuda, Hiroshi Yamasaki, Kotaro Kuwaki, Teiko Takeda, Shigehiko Kitazaki, Osamu Tsuruta, Michio Sata

“…Using a specific enzyme-linked immunosorbent assay, IL-10 concentrations were measured in serum from 62 patients with ulcerative colitis (UC), 43 with Crohn's disease (CD), 25 with other colitides, and 44 normal control subjects. …”
Get full text

Tracheal Resection for Critical Airway Obstruction in Morquio A Syndrome by Claire Frauenfelder, Elizabeth Maughan, Johnny Kenth, Reema Nandi, Simon Jones, Robert Walker, Bill Walsh, Nagarajan Muthialu, Iain Bruce, Richard Hewitt, Colin Butler

“…The relative pathophysiological contributions of an inherent cartilage processing defect versus a mismatch in longitudinal growth between the trachea and the thoracic cage are currently a subject of debate. Enzyme replacement therapy (ERT) and multidisciplinary management continue to improve life expectancy for Morquio A patients by slowing many of the multisystem pathological consequences of the disease but are not as effective at reversing established pathology. …”
Get full text

P-glycoprotein and/or Histone Deacetylase 2 Regulates Steroid Responsiveness in Childhood Nephrotic Syndrome by Harshit Singh, Narayan Prasad, Durga Prasanna Misra, Akhilesh Kumar Jaiswal, Vikas Agarwal

“…Glucocorticoids suppress NFκB-associated coactivator activity by deacetylation of histone by enzyme histone deacetylase (HDAC)-2. Interaction between HDAC2 activity and P-gp expression in childhood NS patients is not clear. …”
Get full text

Association of AGTR1 A1166C and CYP2C9∗3 Gene Polymorphisms with the Antihypertensive Effect of Valsartan by Yi Liu, Xiaomu Kong, Yongwei Jiang, Meimei Zhao, Peng Gao, Xiao Cong, Yongtong Cao, Liang Ma

“…The present study aimed to investigate whether the antihypertensive effect of the ARB drug valsartan was associated with angiotensin II type 1 receptor (AGTR1) gene polymorphism (A1166 C) and cytochrome P450 enzyme 2C9 (CYP2C9) gene polymorphism (CYP2C9∗3). …”
Get full text

Perioperative Treatment with Rivaroxaban and Dabigatran on Changes of Coagulation and Platelet Activation Biomarkers following Left Atrial Appendage Closure by Yao Yao, Yanli Li, Qinchun Jin, Xiaoye Li, Xiaochun Zhang, Qianzhou Lv

“…The biochemical indicators, thrombin-antithrombin complex (TAT), soluble P-selectin (sP-selectin), von Willebrand factor (vWF), and CD40 ligand (CD40L), were measured by enzyme-linked immunosorbent assay. The primary endpoints of this study were the efficacy and safety characteristics of different antithrombotic strategies, including DRT incidence, stroke or transient ischemic attack, systemic embolism, and clinical major and nonmajor bleeding complications during the follow-up of 180 days. …”
Get full text

Anti-Tumor Effect of Rutin on Human Neuroblastoma Cell Lines through Inducing G2/M Cell Cycle Arrest and Promoting Apoptosis by Hongyan Chen, Qing Miao, Miao Geng, Jing Liu, Yazhuo Hu, Lei Tian, Jingkun Pan, Yi Yang

“…Secreted TNF-α level were determined using specific enzyme-linked immunosorbent assay kits. Results. Rutin significantly inhibited the growth of LAN-5 cells and chemotactic ability. …”
Get full text

Clinical value of serum biomarkers, squamous cell carcinoma antigen and apolipoprotein C-II in follow-up of patients with locally advanced cervical squamous cell carcinoma treated... by Yoko Harima, Takuro Ariga, Yuko Kaneyasu, Hitoshi Ikushima, Sunao Tokumaru, Shigetoshi Shimamoto, Takeo Takahashi, Noriko Ii, Kayoko Tsujino, Anneyuko I Saito, Hiroki Ushijima, Takafumi Toita, Tatsuya Ohno

“…ApoC-II was measured using a monoclonal antibody-based enzyme-linked immunosorbent assay, which was developed for this purpose. …”
Get full text

Screening and Characterization of Thermostable Amylase-Producing Bacteria Isolated from Soil Samples of Afdera, Afar Region, and Molecular Detection of Amylase-Coding Gene by Semira Nureddin Yassin, Tamene Milkessa Jiru, Meera Indracanti

“…Studies on the effect of temperature revealed that the crude enzyme had a maximum activity and stability at 75°C, 70°C, and 65°C for isolates M13, M8, and M2, respectively. …”
Get full text

Anti-Myxovirus Resistance Protein-1 Immunoglobulin A Autoantibody in Idiopathic Pulmonary Fibrosis by Toru Arai, Masaki Hirose, Yoshimasa Hamano, Tomoko Kagawa, Akihiro Murakami, Hiroshi Kida, Atsushi Kumanogoh, Yoshikazu Inoue

“…We measured anti-MX1 immunoglobulin (Ig) G, IgA, and IgM autoantibody levels by enzyme-linked immunosorbent assay in serum collected at the time of diagnosis from 71 patients with IPF diagnosed according to the 2018 IPF guideline. …”
Get full text

Advanced Efficient Feature Selection Integrating Augmented Extreme Learning Machine and Particle Swarm Optimization for Predicting Nitrogen Use Efficiency and Yield in Corn by Josselin Bontemps, Isa Ebtehaj, Gabriel Deslauriers, Alain N. Rousseau, Hossein Bonakdari, Jacynthe Dessureault-Rompré

“…The results highlight that integrating soil health indicators such as enzyme activities (β-glucosidase [BG] and N-acetyl-β-D-glucosaminidase [NAG]) and soil proteins into nitrogen management models improves prediction accuracy, leading to enhanced productivity and environmental sustainability. …”
Get full text

The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients by Ade Yonata, Zulkhair Ali, Taufik Indrajaya, Erial Bahar, Ian Effendi, Novadian Suhaimi, Suprapti Suprapti

“…The level of activin A was measured by enzyme-linked immunosorbent assay. Meanwhile, cIMT measurements were collected by B-mode ultrasound imaging. …”
Get full text

Angiotensin receptor neprilysin inhibition versus individualized RAAS blockade: design and rationale of the PARALLAX trial by Rolf Wachter, Sanjiv J. Shah, Martin R. Cowie, Peter Szecsödy, Victor Shi, Ghionul Ibram, Ziqiang Zhao, Jianjian Gong, Sven Klebs, Burkert Pieske

“…In addition, no prior studies have compared sacubitril/valsartan with angiotensin‐converting enzyme inhibitor therapy. We sought to compare the effect of ARNI to background‐medication‐based individualized comparators (BMICs) on N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP), functional capacity [6 min walk distance (6MWD)], symptoms, and quality of life [Kansas City Cardiomyopathy Questionnaire (KCCQ)] in patients with HF and EF >40% in a randomized clinical trial. …”
Get full text

Exploring the NANOG-TET2 interaction interface. Effects of a selected mutation and hypothesis on the clinical correlation with anemias by Claudia Testi, Roberta Piacentini, Roberta Piacentini, Alessandro Perrone, Chiara Bartoli, Daniele Leso, Domitilla Pavia, Elisa Pistolesi, Flavio Scipione, Irene Cotronea, Marco Adinolfi Falcone, Marco Ierani, Alberto Boffi, Lorenzo Di Rienzo

“…In this study, we focused on the computational analysis of a selected single-point mutation identified by a NGS screening panel in the TET2 enzyme classified as “variant of uncertain clinical significance.” …”
Get full text