Unveiling the Potency of Gardenia Extract Against <i>H. pylori</i>: Insights from In Vitro and In Vivo Studies by Pornpen Werawatganone, Duangporn Werawatganon, Nattida Noonak, Maneerat Chayanupatkul, Tanittha Chatsuwan, Naruemon Klaikeaw, Walaisiri Muangsiri, Prasong Siriviriyakul

“…The serum and stomach tissue of the rats were evaluated using enzyme-linked immunosorbent assay, histopathology, and immunohistochemistry. …”
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Early Changes in Tumor Perfusion from T1-Weighted Dynamic Contrast-Enhanced MRI following Neural Stem Cell-Mediated Therapy of Recurrent High-Grade Glioma Correlate with Overall Su... by Prativa Sahoo, Paul Frankel, Julie Ressler, Margarita Gutova, Alexander J. Annala, Behnam Badie, Jana Portnow, Karen S. Aboody, Massimo D’Apuzzo, Russell C. Rockne

“…The aim of this study was to correlate T1-weighted dynamic contrast-enhanced MRI- (DCE-MRI-) derived perfusion parameters with overall survival of recurrent high-grade glioma patients who received neural stem cell- (NSC-) mediated enzyme/prodrug gene therapy. Methods. A total of 12 patients were included in this retrospective study. …”
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Effects of Long-Term Dietary Administration of Kale ( Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats by Katarzyna Papierska, Ewa Ignatowicz, Jadwiga Jodynis-Liebert, Małgorzata Kujawska, Róża Biegańska-Marecik

“…The changes in hepatic antioxidant enzymes and plasma TEAC suggest beneficial effect of kale leaves on the antioxidant status in rats. …”
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Assembly of Genetically Engineered Ionizable Protein Nanocage-based Nanozymes for Intracellular Superoxide Scavenging by Qiqi Liu, Zhanxia Gao, Xiangyun Zhang, Qiannan Duan, Yue Zhang, Adam C. Midgley, Li Jiao, Ruming Liu, Mingsheng Zhu, Deling Kong, Jie Zhuang, Xinglu Huang

“…Abstract Nanozymes play a pivotal role in mitigating excessive oxidative stress, however, determining their specific enzyme-mimicking activities for intracellular free radical scavenging is challenging due to endo-lysosomal entrapment. …”
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Revealing novel protein interaction partners of glyphosate in Escherichia coli by Alix Sarah Aldehoff, Dominique Türkowsky, Patrick Lohmann, Masun Nabhan Homsi, Ulrike Rolle–Kampczyk, Elke Ueberham, Jörg Lehmann, Martin von Bergen, Nico Jehmlich, Sven–Bastiaan Haange

“…Extracellular riboflavin concentrations were elevated upon glyphosate exposure, while intracellular levels remained stable. Beyond the target enzyme EPSPS, thermal proteome profiling indicated an effect of glyphosate on the thermal stability of certain proteins, including AroH and ProA, indicating interactions. …”
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The assessment of marrow adiposity in type 1 diabetic rabbits through magnetic resonance spectroscopy is linked to bone resorption by Wei Li, Wei Wang, Minlan Zhang, Qi Chen, Fengyi Li, Shaojun Li

“…Biochemical analyses covered plasma glucose, enzyme levels, lipid profiles, blood urea nitrogen, creatinine levels, and markers of bone turnover. …”
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Comparison of Effect Sevoflurane-Based Anesthesia and Propofol-Based Anesthesia on the Early Postoperative Renal Function of Living Kidney Transplant Donors: A Randomized Controlle... by Cai J, Kang F, Han M, Huang X, Yan W, Wan F, Li J

“…Kidney injury marker-1 (KIM-1), interleukin-18 (IL-18) and tissue inhibitor of metalloproteinase-2 (TIMP-2) were measured by enzyme-linked immunosorbent assay. Record the cystatin C, glomerular filtration rate, urine output during perioperative period.Results: There were both increases in biomarkers of kidney injury before and 1, 2 and 6 days after the anaesthetic surgery in donors, However, no statistical differences in KIM-1 (P (0.42 pg/mL (95% CI 0.21 to 0.63 pg/mL)) vs S (0.26 pg/mL (95% CI 0.02 to 0.49 pg/mL)), − 0.16 pg/mL (95% CI − 0.48 to 0.16 pg/mL)), IL-18 (P (178.54 pg/mL (95% CI 110.15 to 24693 pg/mL)) vs S (175.86 pg/mL (95% CI 100.35 to 251.38 pg/mL)), − 2.68 pg/mL (95% CI − 105.61 to 100.25 pg/mL)), and TIMP-2 (P (12.88 ng/mL (95% CI 8.69 to 17.07 ng/mL)) vs S (14.85 ng/mL (95% CI 10.23 to 19.46 ng/mL)), 1.97 ng/mL (95% CI − 4.30 to 8.23 ng/mL)) concentration changes between the two types of anaesthesia.Conclusion: There was no difference between sevoflurane and propofol anaesthesia on postoperative changes in biomarkers of renal injury in living kidney transplant donors.Keywords: general anesthetics, sevoflurane, propofol, renal transplantation…”
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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The aims of this work were i) to determine whether it is possible to create recombinant K. phaffii K51 clones overexpressing functionally active proteinase K from T. album and ii) to analyze the enzymatic activity of the resulting recombinant enzyme. The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Ion suppression correction and normalization for non-targeted metabolomics by Iqbal Mahmud, Bo Wei, Lucas Veillon, Lin Tan, Sara Martinez, Bao Tran, Alexander Raskind, Felice de Jong, Yiwei Liu, Jibin Ding, Yun Xiong, Wai-kin Chan, Rehan Akbani, John N. Weinstein, Chris Beecher, Philip L. Lorenzi

“…To demonstrate a routine application of the Workflow, we employ the Workflow to study ovarian cancer cell response to the enzyme-drug L-asparaginase (ASNase). The IROA-normalized data reveal significant alterations in peptide metabolism, which have not been reported previously. …”
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Growth performance, intestinal health, and non-specific immunity were significantly affected by feeding different compound lactic acid bacteria supplementation in sea urchin (Stron... by Yuntian Zhang, Yuntian Zhang, Rongwei Zhang, Rongwei Zhang, Yi Chen, Yi Chen, Zhixu Guo, Zhixu Guo, Xiangyu Meng, Xiangyu Meng, Yuzhe Han, Yuzhe Han, Xiaoran Zhao, Xiaoran Zhao, Tongjun Ren, Tongjun Ren

“…Notably, compound lactic acid bacteria improved digestive enzyme activities in the intestine and reduced the feed conversion ratio (FCR). …”
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A novel platform for engineered AAV-based vaccines by Sabrina Babutzka, Miranda Gehrke, Anastasia Papadopoulou, Maria Diedrichs-Möhring, Maria Giannaki, Lena Hennis, Bastian Föhr, Cale Kooyman, Andreas Osterman, Evangelia Yannaki, Gerhild Wildner, Hermann Ammer, Stylianos Michalakis

“…This engineering endowed AAV.RBDs with SARS-CoV-2-like properties, such as angiotensin-converting enzyme 2 receptor affinity. In line with this, AAV.RBDs were neutralized by sera from human donors vaccinated against SARS-CoV-2. …”
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Comprehensive metabolite profiling and evaluation of anti-nociceptive and anti-inflammatory potencies of Nypa fruticans (Wurmb.) leaves: Experimental and in-silico approaches by Farhana Islam, Md. Aktaruzzaman, Md. Tarikul Islam, Fariya Islam Rodru, Saquiba Yesmine

“…The identified compounds were subsequently evaluated by computational studies against the cyclooxygenase-2 enzyme. EENF demonstrated significant antioxidant activity in both the DPPH scavenging assay (IC50: 105.18 μg/mL) and the reducing power assay (RC50:1752.76 μg/mL). …”
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Molecular Characterization, Oxidative Stress-Mediated Genotoxicity, and Hemato-Biochemical Changes in Domestic Water Buffaloes Naturally Infected with <i>Trypanosoma evansi</i> Und... by Waqas Ahmad, Muhammad Yasin Tipu, Muti ur Rehman Khan, Haroon Akbar, Aftab Ahmad Anjum, Muhammad Ovais Omer

“…Alongside significant (<i>p</i> < 0.05) reductions in red cell indices, a marked elevation in leukocyte counts and serum hepatic enzyme levels was recorded in the affected buffaloes. (4) Conclusion: <i>T. evansi</i> isolates of buffaloes from Multan, Pakistan, have genetic similarities to Indian isolates. …”
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Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening by Wei Pu, Jinhui Feng, Jiuzhou Chen, Jiao Liu, Xuan Guo, Lixian Wang, Xiaojia Zhao, Ningyun Cai, Wenjuan Zhou, Yu Wang, Ping Zheng, Jibin Sun

“…Subsequently, the SerRF104I-based biosensor was employed for high-throughput screening of the superior enzyme mutants of l-homoserine dehydrogenase and γ-glutamyl kinase, which are critical enzymes in the biosynthesis of l-threonine and l-proline, respectively. …”
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Free Fatty Acids in CSF and Neurological Clinical Scores: Prognostic Value for Stroke Severity in ICU by Sayed Gaber, Sherine Ibrahim ElGazzar, Mahmoud Qenawi, Nora Ismail Mohamed Abbas

“…The FFA levels were measured using the sensitive enzyme-based colorimetric method. The NIHSS, GCS, and mRS were evaluated at admission and at 30 days. …”
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Inhibition Mechanism of Pleurotus citrinipileatus Sing. Ergothioneine on Pancreatic Lipase Activity by Zhangchen XIA, Xiaohui MENG, Ru FANG, Yanqing MAO, Junwen CHENG, Yanbin WANG, Juan XU, Liang HE

“…The interaction between Pleurotus citrinipileatus Sing. ergothioneine (PCEGT) and pancreatic lipase (PL) was studied by analyzing multiple spectroscopies including enzyme kinetics, ultraviolet absorption (UV), fluorescence spectroscopy, synchronous fluorescence spectroscopy and 3D fluorescence spectroscopy as well as the PCEGR-PL interaction mechanism by molecular docking in order to clarify its inhibitory mechanism. …”
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Evaluation of Inflammatory Cytokine Secretion by Human Alveolar Macrophages by J. E. Losa García, F. M. Rodríguez, M. R. Martín de Cabo, M. J. García Salgado, J. P. Losada, L. G. Villarón, A. J. López, J. L. P. Arellano

“…IL-1β, TNF-α, IL-6 and IL-8 levels were measured in BAL fluid and culture supernatant using specific enzyme-linked immunosorbent assays. The substance found to stimulate the secretion of inflammatory cytokines to the greatest extent was LPS at a concentration of 10 μg/ml. …”
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Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis by Liming Liu, Cuiqing Zhao, Ying Yang, Xiaoxia Kong, Tuo Shao, Li Ren, Xinyu Zhuang, Baishuang Yin, Gerald Dryden, Craig McClain, Weimin Luan, Wenke Feng

“…Lipolysis index and adipose lipolytic enzymes were determined. In addition, 3T3-L1 cells were pretreated with IL-6, followed by recombinant human FGF21 (rhFGF21) treatment; lipolysis was assessed. …”
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Comparative analysis of the primary structure and production of recombinant poly(ADP-ribose)polymerase 1 of long-lived Heterocephalus glaber by K. N. Naumenko, A. R. Nurislamov, K. D. Nazarov, V. S. Fishman, A. A. Popov, I. O. Petruseva, A. N. Evdokimov, O. I. Lavrik

“…The central regulator of the DNA repair process is the enzyme poly(ADP-ribose)polymerase 1 (PARP1). PARP1 catalyzes the synthesis of poly(ADPribose) polymer (PAR) upon DNA damage using nicotinamide adenine dinucleotide (NAD+) as a substrate. …”
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Antioxidant, Anti-Tyrosinase, and Wound-Healing Capacities of Soy Protein Hydrolysates Obtained by Hydrolysis with Papaya and Cantaloupe Juices Showing Proteolytic Activity by Thi-Phuong Nguyen, Quang Thai Le, Mai Linh Thi Tran, Kim Nhung Ta, Khoa Thi Nguyen

“…A higher DH (about 26%) was obtained by the hydrolysis with cantaloupe juice at the same juice to substrate ratio and treatment conditions. Papain used at the enzyme to substrate ratio of 0.625:2 ( w/w ) broke down SPI in a similar DH as papaya juice at the juice to substrate ratio of 2.5:2 ( v/w ). …”
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