Study Imbalance of Some Cytokines and Vaginitis Agents in Women with Recurrent Abortion Women by Muna Hamid Al-sallami, Samara Abd Al-Hameed Ouda Al-Salihi, Mayyada Farhan Darweesh

“…Two milliliters of blood samples were collected from all participants to measure interleukin-17 (IL-17), interleukin-23 (IL-23), and interleukin-10 (IL-10) levels by enzyme-linked immunosorbent assay (ELISA). High vaginal swabs were taken from all patients, cultured on differential media for 24 h at 37°C, and then positive bacterial growth re-culturing to obtain a pure single colony. …”
Get full text

Programmable Multiplexed Nucleic Acid Detection by Harnessing Specificity Defect of CRISPR‐Cas12a by Xin Guan, Rui Yang, Jiongyu Zhang, Jeong Moon, Chengyu Hou, Chong Guo, Lori Avery, Danielle Scarola, Daniel S. Roberts, Rocco LaSala, Changchun Liu

“…By harnessing the specificity defect of the CRISPR‐Cas12a enzyme, a dual‐mode detection strategy is designed, which includes approximate matching and precise querying of target sequences and develop a programmable multiplexed nucleic acid assay. …”
Get full text

Relationship between BDNF content in cord blood and early neurobehavior in newborns with subclinical hypothyroidism during pregnancy: a preliminary study by Hui Xu, Ya-Nan Du, Shuai Yang, Yu-Wen Guo

“…BDNF levels in umbilical cord blood were measured using the Enzyme-Linked Immunosorbent Assay method.ResultsThe results indicated that neonates with SCH during pregnancy had lower total NBNA scores, behavioral ability, passive muscle tone, active muscle tone, primitive reflexes, general assessment, and lower levels of cord blood BDNF compared to healthy controls. …”
Get full text

Role and Effective Therapeutic Target of Gut Microbiota in Heart Failure by Qiujin Jia, Hao Li, Huan Zhou, Xiaonan Zhang, Ao Zhang, Yingyu Xie, Yanyang Li, Shichao Lv, Junping Zhang

“…At the same time, regulating intestinal microflora through diet, probiotics, antibiotics, fecal transplantation and microbial enzyme inhibitors has grown up to be a potential treatment for many metabolic disorders.…”
Get full text

Comparison of VIDAS and Radioimmunoassay Methods for Measurement of Cortisol Concentration in Bovine Serum by Daniela Proverbio, Roberta Perego, Eva Spada, Giada Bagnagatti de Giorgi, Angelo Belloli, Davide Pravettoni

“…The VIDAS cortisol test is an enzyme-linked fluorescent assay designed for the MiniVidas system. …”
Get full text

Factors associated with early-onset androgenetic alopecia: A scoping review. by Li-Ping Liu, Mary Adumo Wariboko, Xiao Hu, Zi-Han Wang, Qian Wu, Yu-Mei Li

“…Early-onset AGA may also be associated with hormonal profiles, 5α-reductase enzyme activity, androgen receptor genes, and some susceptibility loci. …”
Get full text

ALDOA contributes to colorectal tumorigenesis and metastasis by targeting YAP by Liang Sun, Ting Lu, Linhua Jiang, Huihui Yao, Qixuan Xu, Jie Sun, Xiaoqin Yang, Songbing He, Xinguo Zhu

“…In this view, Aldolase A (ALDOA), a key glycolytic enzyme, has been validated as a candidate oncogene in several cancers. …”
Get full text

Expression of Angiogenesis Regulatory Proteins and Epithelial-Mesenchymal Transition Factors in Platelets of the Breast Cancer Patients by Hui Han, Fang-Li Cao, Bao-Zhong Wang, Xue-Ru Mu, Guang-Yao Li, Xiu-Wen Wang

“…Vascular endothelial growth factor (VEGF), platelet-derived growth factor BB (PDGF-BB), thrombospondin-1 (TSP-1), platelet factor 4 (PF4), and transforming growth factor-β1 (TGF-β1) were measured by enzyme-linked immunosorbent assay. Angiogenesis-associated expression of VEGF (2.1 pg/106 platelets versus 0.9 pg/106 platelets, P < 0.001), PF4 (21.2 ng/106 platelets versus 10.2 ng/106 platelets, P < 0.001), PDGF-BB (42.9 pg/106 platelets versus 19.1 pg/106 platelets, P < 0.001), and TGF-β1 (15.3 ng/106 platelets versus 4.3 ng/106 platelets, P < 0.001) differed in the PP samples of cancer and control subjects. …”
Get full text

APOBEC3C‐mediated NF‐κB activation enhances clear cell renal cell carcinoma progression by Nora Hase, Danny Misiak, Helge Taubert, Stefan Hüttelmaier, Michael Gekle, Marcel Köhn

“…Here, we unveil that the enhanced expression of the RNA‐binding protein DNA dC → dU‐editing enzyme APOBEC‐3C (APOBEC3C; also known as A3C) in ccRCC tissue and ccRCC‐derived cell lines serves as a catalyst for tumor growth by amplifying nuclear factor‐kappa B (NF‐κB) activity. …”
Get full text

Angiogenic Response to Major Lung Resection for Non-Small Cell Lung Cancer with Video-Assisted Thoracic Surgical and Open Access by Calvin S. H. Ng, Song Wan, Randolph H. L. Wong, Anthony M. H. Ho, Anthony P. C. Yim

“…Blood samples were collected preoperatively and postoperatively on days (POD) 1 and 3 for enzyme linked immunosorbent assay determination of angiogenic factors. …”
Get full text

Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells by Si-Zhe Li, Xin-Xing Jin, Xiao-Lei Ge, Ya-Gang Zuo, Hong-Zhong Jin

“…TSLP levels in sera and blister fluids were measured by enzyme-linked immunosorbent assay. The TSLP expression in the BP lesional skin was detected by immunohistochemical staining. …”
Get full text

Ameliorative Effects of Polygonatum kingianum Polysaccharides on Lipid Metabolism Disorders and Brain Function Impairments in Obese Mice by XU Yuncong, YAN Qiaojuan, ZHU Chunhua, YANG Shaoqing, LI Houjiang, JIANG Zhengqiang

“…To study the effects of Polygonatum kingianum polysaccharides (PKP) on lipid metabolism disorders and brain function impairments in obese mice, the obese mice were treated with PKP, prepared by enzyme-assisted extraction, for 12 weeks. Changes in body mass and blood lipids, as well as pathological changes in liver and adipose tissue were observed. …”
Get full text

Analysis of Glucocorticoids as Potential Adulterants in Cosmetic Products: A Dual Approach for Qualitative and Quantitative Evaluation Based on ELISA and HPLC-MS Methods by Seyedeh Rojin Shariati Pour, Afsaneh Emamiamin, Martina Zangheri, Donato Calabria, Massimo Guardigli, Emanuele Porru, Jessica Fiori, Mara Mirasoli

“…This reference method was then compared with an enzyme-linked immunosorbent assay (ELISA). Indeed, immunological techniques allow for rapid, low-cost, and sensitive detection of target analytes even in complex matrices, and they can be performed with simple instrumentation and by non-skilled personnel. …”
Get full text

Manganese biofortification in grapevine by foliar spraying improves volatile profiles of Cabernet Sauvignon grapes and wine sensory traits by Ruihua Ren, Miaomiao Wang, Lijian Zhang, Fuxian Ren, Bowei Yang, Huangzhao Chen, Zhenwen Zhang, Qingqing Zeng

“…Manganese (Mn) is involved in plant metabolism as an enzyme cofactor. However, the role of Mn in the formation of volatile compounds in grapes has rarely been studied. …”
Get full text

Neuroprotective Role of Transchalcone in Parkinson‘s Disease through AMP-activated Protein Kinase-mediated Signaling Pathway by Yao Cheng, Shaik Althaf Hussain, Turki Mayudh Alrubie, Xiaomin Zhang

“…Striatal dopamine and cytokines (interleukin 1-beta [IL-1 β], IL-10) and p65-nuclear factor kappa-B (NF-κB) levels were measured with enzyme-linked immunosorbent assay. Mitochondrial function was evaluated by fluorometric techniques. …”
Get full text

Targeting MAO-B with Small-Molecule Inhibitors: A Decade of Advances in Anticancer Research (2012–2024) by Iyman Alsaad, Diana M. A. Abdel Rahman, Ola Al-Tamimi, Shayma’a A. Alhaj, Dima A. Sabbah, Rima Hajjo, Sanaa K. Bardaweel

“…Monoamine oxidase B (MAO-B) is a key enzyme in the mitochondrial outer membrane, pivotal for the oxidative deamination of biogenic amines. …”
Get full text

Serum Vascular Endothelial Growth Factor Levels before and after Intravitreous Ranibizumab Injection for Retinopathy of Prematurity by Xuting Chen, Lin Zhou, Qi Zhang, Yu Xu, Peiquan Zhao, Hongping Xia

“…Serum VEGF level was measured by the enzyme-linked immunosorbent assay (ELISA). Results. …”
Get full text

Could serum Raftlin and GPER-1levels be new biomarkers for early detection of non-small cell lung cancer? by Aykut Eliçora, Berrak Güven, Hüseyin Engin, Gokcen Tugba Çevik, Hüseyin Fatih Sezer

“…GPER-1 and Raftlin levels were measured by Enzyme-Linked Immunosorbent Assay (ELISA) in blood samples taken from patients diagnosed with lung cancer and healthy volunteers. …”
Get full text

Zinc oxide seed priming enhances drought tolerance in wheat seedlings by improving antioxidant activity and osmoprotection by Rasha M. El-Shazoly, A. A. Othman, Muhammad Saqlain Zaheer, Ahmed F. Al-Hossainy, Dalia A. Abdel-Wahab

“…Zinc is an essential element for several enzymes involved in metabolism, cell elongation, preservation of the strength and integrity of cell membranes, seed development, and resistance to environmental stress. …”
Get full text

Matrix metalloproteinase-3 is a potent prognostic factor associated with cell proliferation and migration in prostate cancer by Ai Sato, Kiyoshi Takagi, Mio Yamaguchi-Tanaka, Jotaro Okushima, Yuto Yamazaki, Akihiro Ito, Takashi Suzuki

“…Matrix metalloproteinase-3 (MMP3) is an enzyme that degrades several ECM components and is implicated in human malignancies. …”
Get full text