Oral Administration of Semicarbazide Limits Weight Gain together with Inhibition of Fat Deposition and of Primary Amine Oxidase Activity in Adipose Tissue by Josep Mercader, Zsuzsa Iffiú-Soltész, Sandy Bour, Christian Carpéné

“…An enzyme hitherto named semicarbazide-sensitive amine oxidase (SSAO), involved in the oxidation of primary amines, is abundantly expressed in adipocytes. …”
Get full text

Dapagliflozin inhibits ferroptosis to improve chronic heart failure by regulating Nrf2/HO-1/GPX4 signaling pathway. by Jing Zhang, Xuefeng Chen, Shilin Lv, Qingqing Hao

“…Echocardiography was used to assess cardiac function, HE staining to evaluate pathological changes in the heart, Prussia blue staining to observe iron ions in myocardial tissue, and enzyme-linked immunosorbent assay (ELISA) to determine serum levels of the inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) at the end of week 12 and/or the end of week 16. …”
Get full text

Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis by Guo-Ping Chen, Jian Yang, Guo-Feng Qian, Wei-Wei Xu, Xiao-Qin Zhang

“…Geranylgeranyl transferase-I (GGTase-I) is an essential enzyme mediating posttranslational modification, especially the geranylgeranylation of small GTPase, Rac1. …”
Get full text

Glial cell line-derived neurotrophic factor in patients with age-related cataract by A.A. Shpak, A.B. Guekht, T.A. Druzhkova, A.A. Troshina, N.V. Gulyaeva

“…The concentration of GDNF was measured using an enzyme immunoassay. Results. The concentration of GDNF in the AH was 88.9±46.9 (2.0–212.5) pg/ml, in the BS – 201.1±44.6 (103.2–287.2) pg/ml, in the LF – 343.6±133.7 (172.8–683.0) pg/ml. …”
Get full text

Evaluation of Inflammation-Related Proteins in Multiple Sclerosis Disease with Relapses and Remissions by Nisa Hocaoğlu, Nur Damla Korkmaz, Birsen Elibol, Zehra Cemre Karakayalı, Şule Terzioğlu-Uşak, Azize Esra Gürsoy

“…In addition, the protein levels of the NLRP3 and ASC were determined from serum samples by enzyme-linked immunosorbent assay. Results: The expression of the NLRX1 gene was significantly decreased in the patient groups compared to the controls. …”
Get full text

The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy by Dan Pan, Mengting Jiang, Guoxian Tao, Jinmei Shi, Zhiwei Song, Ren Chen, Dongguo Wang

“…The expression of InsP3R protein in the liver, and serum levels of Ca2+, glycocholic acid and bile acid were detected.Results InsP3R mRNA and protein were significantly lower in the ICP model group compared to the normal group, as determined by qPCR and immunohistochemistry, respectively. Serum enzyme-linked immunosorbent assay results revealed significantly higher levels of glycocholic acid and bile acid in the ICP model group compared to the normal group, while Ca2+ levels were significantly lower. …”
Get full text

Bone marrow transplantation reverses metabolic alterations in multiple sulfatase deficiency: a case series by Nishitha R. Pillai, Ning Liu, Xiyuan Li, Xiqi Li, Rebecca Ahrens-Nicklas, Laura Adang, Julie B. Eisengart, Grace Bronken, Ashish Gupta, Troy C. Lund, Chester B. Whitley, Sarah H. Elsea, Paul J. Orchard

“…The activation of all these cellular sulfatases is dependent on the presence of the formylglycine-generating enzyme, which is encoded by the SUMF1 gene. Disease-causing homozygous or compound heterozygous variants in SUMF1 result in MSD. …”
Get full text

The Effect of Lipids on the Structure and Function of Egg Proteins in Response to Pasteurization by Hao Yang, Qiang Shi, Zhongliang Wang, Xiao Chen, Fangfang Min, Xuanyi Meng, Ping Tong, Yong Wu, Hongbing Chen

“…The protein structural changes were analyzed using polypropylene gel electrophoresis, circular dichroism, ultraviolet absorption spectra, and exogenous fluorescence spectra, and the functional properties were assessed through solubility measurements and particle size analysis, while protein antigenicity was evaluated using an enzyme-linked immunosorbent assay. All the results revealed that oleic acid had the most significant effect on proteins’ secondary and tertiary structures, particularly affecting ovalbumin and ovotransferrin. …”
Get full text

Seroprevalence of Avian Influenza in Guinea Fowls in Some Districts in the Upper East Region of Ghana by Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe

“…Blood samples were collected from 397 guinea fowls that are over 4 weeks old, and seroprevalence was determined using ID Screen Influenza A Antibody Competition Enzyme‐linked immunosorbent assay (ELISA). The study analysed demographic factors such as sex, age and source of birds, employing statistical methods to establish associations. …”
Get full text

Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model by Amin Arsang, Seyed Davar Siadat, Masoumeh Saberpour, Farshad Nojoomi

“…Serum samples were acquired on days 0, 14, and 28 post-immunization, then IgM and IgG levels were assayed by enzyme-linked immunosorbent assay. Results: The concentrations of PRP, dLOS, and endotoxin were 1160 mg/L, 440 μg/mL, and 1450 EU/mL, respectively. …”
Get full text

Improved Antibacterial Properties of Fermented and Enzymatically Hydrolyzed Bee Pollen and Its Combined Effect with Antibiotics by Vaida Damulienė, Vilma Kaškonienė, Paulius Kaškonas, Rūta Mickienė, Audrius Maruška

“…Bee pollen hydrolysis was performed using commercial enzymes, including enzyme mixtures as well as pure enzymes. …”
Get full text

Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophore... by Mohammed Al Bratty, Hassan A. Alhazmi, Sadique A. Javed, Zia Ur Rehman, Asim Najmi, Karam A. El-Sharkawy

“…The present study may be used as a reference for the investigation of protein-metal ion, drug-protein, drug-metal ion, and enzyme-metal ion interactions and may be helpful to provide preliminary insight into the new metal-based drug development.…”
Get full text

Enhancing Venous Thromboembolism Risk Prediction through Novel Biomarkers and Inflammatory Indicators by Zainab Wahab Al-Maaroof

“…Plasma was collected for biomarker analysis in the following: D-dimer, thrombin generation, factor VIII levels, and inflammatory markers using enzyme-linked immunosorbent assay (ELISA) (CRP/IL-6/miRNAs), CAT, and real-time polymerase chain reaction. …”
Get full text

Inhibition of Cathepsin S Produces Neuroprotective Effects after Traumatic Brain Injury in Mice by Jianguo Xu, Handong Wang, Ke Ding, Xinyu Lu, Tao Li, Jiawei Wang, Chunxi Wang, Jian Wang

“…It has long been regarded as an enzyme that is primarily involved in general protein degradation. …”
Get full text

Leptin Promoted IL-17 Production from ILC2s in Allergic Rhinitis by Yanhui Wen, Qingxiang Zeng, Xi Luo, Renqiang Ma, Yiquan Tang, Wenlong Liu

“…Serum leptin levels were measured, and their correlation with the frequency of IL-17+ ILC2 cells was analyzed using enzyme-linked immunosorbent assay (ELISA) and flow cytometry. …”
Get full text

Immunomodulatory effect of hepatitis B virus (HBV) antigens and MRSA antigens on immune response by Entidhar A. Hadi, Mohammad Abd-Kadhum Al-Saadi, Kaiser N. Madlum

“…The immune response against MRSA somatic antigens was assessed by measurement of interferon-γ, interleukin-4, and interleukin-5 by enzyme-linked immunosorbent assay technique. Results: The results indicated a significant increase (P < 0.05) in the mean value of IFN-γ in group II (125.9995 pg/mL) and III group (133.6504 pg/mL) and group IV (132.0271 pg/mL) as compared with control (group I) (49.6203 pg/mL). …”
Get full text

Divergent modulation of activated protein C pleiotropic functions by antibodies that differ by a single amino acid by Derek S. Sim, Meenal Shukla, Cornell R. Mallari, José A. Fernández, Xiao Xu, Doug Schneider, Maxine Bauzon, Terry W. Hermiston, Laurent O. Mosnier

“…This study illustrates that APC is highly sensitive to non–active site targeting that can lead to unpredictable changes in its activity profile of this pleiotropic enzyme. Furthermore, this study demonstrates the ability to modify APC functions to advance the potential development of APC-targeted antibodies as therapeutics for the treatment of diseases including trauma bleeding, hemophilia, ischemia, and sepsis.…”
Get full text

In Vitro and In Silico Cytotoxic and Antibacterial Activities of a Diterpene from Cousinia alata Schrenk by Almira Zhanzhaxina, Yerlan Suleimen, Ahmed M. Metwaly, Ibrahim H. Eissa, Eslam B. Elkaeed, Raigul Suleimen, Margarita Ishmuratova, Kydyrmolla Akatan, Walter Luyten

“…The presence of the essential pharmacophoric features of histone deacetylase (HDAC) inhibitors in the structure of grindelic acid encouraged us to run a molecular docking study against the HDAC enzyme to understand its mechanism of action on a molecular level. …”
Get full text

Seroprevalence of Peste des Petits Ruminants and Contagious Caprine Pleuropneumonia Coinfections in Goats in Kwale County, Kenya by George Lugonzo, George Gitao, Lilly Bebora, Harrison Osundwa Lutta

“…Herein, the competitive enzyme-linked immunosorbent assay (cELISA) and the latex agglutination test (LAT) were used to determine the coinfections of PPR and CCPP in goats in Kwale County on Kenya’s South Coast. …”
Get full text

Effects of cattle manure and sludge vermicompost on nutrient dynamics and yield in strawberry cultivation with distinct continuous cropping histories in a greenhouse by Xiaofeng Bai, Wei Lu, Jin Xu, Qingyun Li, Zhanjun Xue, Xin-Xin Wang

“…In both substrate types, there was a substantial increase in microbial population and enzyme activity, accompanied by a significant decrease in phenolic acid content. …”
Get full text