Biochemical Pattern of Methylmalonyl-CoA Epimerase Deficiency Identified in Newborn Screening: A Case Report by Evelina Maines, Roberto Franceschi, Francesca Rivieri, Giovanni Piccoli, Björn Schulte, Jessica Hoffmann, Andrea Bordugo, Giulia Rodella, Francesca Teofoli, Monica Vincenzi, Massimo Soffiati, Marta Camilot

“…Methylmalonyl-CoA epimerase enzyme (MCEE) is responsible for catalyzing the isomeric conversion between D- and L-methylmalonyl-CoA, an intermediate along the conversion of propionyl-CoA to succinyl-CoA. …”
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Altered CD73-Adenosine Signaling Linked to Infection in Patients undergoing hemodialysis by Xiang F, Zhang Z, Nie Y, Cao X, Li Y, Chen X, Gong S, Zou J, Teng J, Ding X, Shen B

“…Fangfang Xiang,1– 4,* Zhen Zhang,1,* Yuxin Nie,1 Xuesen Cao,1 Yang Li,1 Xiaohong Chen,1 Shaomin Gong,1 Jianzhou Zou,1 Jie Teng,1 Xiaoqiang Ding,1– 4 Bo Shen1– 4 1Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China; 2Shanghai Key Laboratory of Renal Disease and Blood Purification, Shanghai, People’s Republic of China; 3Shanghai Medical Center of Kidney, Shanghai, People’s Republic of China; 4Shanghai Institute of Kidney and Dialysis, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bo Shen, Department of Nephrology, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, People’s Republic of China, Email shen.bo@zs-hosptial.sh.cnPurpose: Infection is the most common cause of hospitalization and the second most common cause of mortality among patients undergoing hemodialysis (HD). The enzyme CD73, a cell surface 5′-nucleotidase, regulates the balance between pro-inflammatory nucleotides and anti-inflammatory adenosine. …”
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Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China by Le Chang, Junpeng Zhao, Fei Guo, Huimin Ji, Lu Zhang, Xinyi Jiang, Lunan Wang

“…Background. Enzyme-linked immunosorbent assay (ELISA) is the only serological method approved for blood screening in China. …”
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Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity by Biran Zhu, Zequan Zhang, Ying Xie, Min Huang, Yu Chen, Yong Yang, Xiongjie Shi, Jian Han, Lihua Yang, Min Zhao

“…Furthermore, histopathological analysis, western blot (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence results showed that exposure to BPS led to testicular damage, inflammation, activation of the p38 and ERK MAPK pathways, and disruption of the blood-testis barrier (BTB). …”
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Mn-doped ZnO thin films as a platform for reagentless uric acid biosensor by Bilasini Devi Naorem, Jatinder Pal Singh, Babita Sharma, Satyam Garg, Athira C, Hashima Sherin, Mahima Momaliya, Muskan, Shubhi Sahu, Arijit Chowdhuri, Mallika Verma, Monika Tomar, Neha Batra

“…In order to immobilise the uricase enzyme, the 7 % Mn doped composition was used, creating a highly sensitive and focused uric acid detection platform. …”
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The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury by Xiao Zhao, Han Han, Yinting Song, Mei Du, Mengyu Liao, Xue Dong, Xiaohong Wang, Ferenc Kuhn, Annette Hoskin, Heping Xu, Hua Yan

“…Vitreous fluid was collected, and levels of growth factors and cytokines were measured by enzyme-linked immunosorbent assay (ELISA). On day 28 after OGI, B scan examination and histological examination were performed to evaluate intravitreal proliferation and formation of epiretinal fibrosis. …”
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Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress by Jianqin Zhang, Dianfeng Zheng, Naijie Feng, Aaqil Khan, Rui Deng, Jian Xiong, Linchong Ding, Zhiyuan Sun, Jiahuan Li, Xiaohui Yang, Chen Wu

“…The current findings indicate that GR24 effectively mitigates the adverse impact salt stress by regulating antioxidant enzyme activity and endogenous hormone balance.…”
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Synthesis, Antibacterial, Antioxidant, and Molecular Modeling Studies of Novel [2,3′-Biquinoline]-4-Carboxylic Acid and Quinoline-3-Carbaldehyde Analogs by Zeleke Digafie, Yadessa Melaku, Zerihun Belay, Rajalakshmanan Eswaramoorthy

“…The molecular docking analysis of the compounds against E. coli DNA gyrase was computed to investigate the binding mode of the compounds within the active site of the enzyme. In this regard, a new series of [2,3′-biquinoline]-4-carboxylic acid and quinoline-3-carbaldehyde analogs were synthesized by utilization of Vilsmeier–Haack, Doebner, nucleophilic substitution, and hydrolysis reactions. …”
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Human cytomegalovirus (HCMV) trends in Sri Lanka: insights from a hospital-based seroprevalence analysis by Narapity Pathirannehalage Sunil-Chandra, Madawala Vithanage Madusha Lakmali Jayasundara, Maththe Gama Ralalage Shobha Sanjeewani Gunathilaka, Suduwa Dewage Himal Suminda

“…Methods A total of 820 blood samples obtained from 820 participants of selected low and high-risk populations in Sri Lanka, namely; hospital inpatients (n = 200), antenatal women (n = 80), blood donors (n = 80), cancer patients (n = 200), Sexually Transmitted Disease (STD) clinic attendees (n = 160) and Chronic Kidney Disease (CKD) patients on haemodialysis (n = 100) were tested for IgG antibodies against HCMV by Enzyme Linked Immunosorbent Assay (ELISA). Statistical analysis was performed using IBM SPSS Statistics version 30 (IBM® Corporation, New York, USA). …”
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The MSDIN family in amanitin-producing mushrooms and evolution of the prolyl oligopeptidase genes by Hong Luo, Qing Cai, Yunjiao Lüli, Xuan Li, Rohita Sinha, Heather E. Hallen-Adams, Zhu L. Yang

“…Prolyl oligopeptidase B (POPB), a key enzyme in the biosynthetic pathway, was used in phylogenetic reconstruction to infer the evolutionary history of the genes. …”
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Enhanced Activity by NKCC1 and Slc26a6 Mediates Acidic pH and Cl− Movement after Cardioplegia-Induced Arrest of db/db Diabetic Heart by Minjeong Ji, Seok In Lee, Sang Ah Lee, Kuk Hui Son, Jeong Hee Hong

“…Interestingly, expression of Slc26a6, as well as carbonic anhydrase IV as a supportive enzyme of Slc26a6, was increased in db/db cardiac strips compared to wild type cardiac strips. …”
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Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson’s Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway by Dengjun Guo, Jie Zhou, Meng Zhang, Reyisha Taximaimaiti, Xiaoping Wang, Hai Wang

“…High-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA) were used to determine neurotransmitters and metabolites, inflammatory factors TNF-α and IL-1β, oxidative stress-related markers SOD, MDA, and GSH in striatum tissues. …”
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The interplay between RNA m6A modification and radiation biology of cancerous and non-cancerous tissues: a narrative review by Yajia Cheng, Yue Shang, Shuqin Zhang, Saijun Fan

“…N6-methyladenosine (m6A), a prevalent modification on coding and non-coding RNAs, is a key epigenetic mark established by a set of evolutionarily conserved enzymes. The interplay between m6A modification and radiobiology of cancerous and non-cancerous tissues merits in-depth investigation. …”
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CD2AP deficiency aggravates Alzheimer’s disease phenotypes and pathology through p38 MAPK activation by Yan-Yan Xue, Zhe-Sheng Zhang, Rong-Rong Lin, Hui-Fen Huang, Ke-Qing Zhu, Dian-Fu Chen, Zhi-Ying Wu, Qing-Qing Tao

“…Then, we generated and characterized an APP/PS1 mouse model with neuron-specific Cd2ap deletion, using immunoblotting, immunofluorescence, enzyme-linked immunosorbent assay, electrophysiology and behavioral tests. …”
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The ILE462VAL polymorphism of the cytochrome P450 CYP1A1 gene among Tundra Nenets in Yamalo-Nenets Autonomous Okrug, Nganasans in the Taimyr Peninsula and Russians in Siberia by R. P. Tiis, L. P. Osipova, T. V. Churkina, L. E. Tabikhanova, D. V. Lichman, E. N. Voronina, M. L. Filipenko

“…This substitution results in a two- fold increase in enzyme activity, which leads to accumulation of active intermediates and increases the risk of DNA mutations and chemically induced carcinogenesis. …”
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The Effect of A2E on the Ca2+-PKC Signaling Pathway in Human RPE Cells Exposed to Blue Light by Maomei Luo, Shu Wang, Yun Tang, Chun Zeng, Shanjun Cai

“…The intracellular levels of inositol triphosphate (IP3) and diacylglycerol (DAG) were measured by enzyme-linked immunosorbent assay (ELISA). Intracellular PKC activity was measured with a nonradioactive nuclide assay. …”
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TAp63γ is the primary isoform of TP63 for tumor suppression but not development by Xinbin Chen, Wenqiang Sun, Xiangmudong Kong, Xin Ming, Yanhong Zhang, Wensheng Yan, Shakur Mohibi, Mingyi Chen, Keith Mitchell, Jin Zhang

“…Single-cell RNA-seq revealed that loss of p63γ increases the expression of SCD1, a rate-limiting enzyme for synthesis of monounsaturated fatty acids, leading to altered lipid homeostasis. …”
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The serum level of sclerostin decreases in radiographic axial spondyloarthritis patients with fatty lesions by Xuegang Li, Haijian Jiang, Xu Wang, Shuping Zhong

“…Serum levels of bone turnover markers, including sclerostin, osteoprotegerin (OPG), procollagen I N-terminal propeptide (PINP), cross linked C-telopeptide of type I collagen (CTX-I), osteocalcin (OC), were measured using enzyme-linked immunosorbent assays. Results There were no significant differences between two groups in terms of gender, age, body mass index (BMI), duration, smoking, HLA-B27 positivity rate, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), BASDAI, ASDAS-ESR, ASDAS-CRP, biological disease-modifying anti-rheumatic drugs (bDMARDs) rate. …”
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Stereospecific α-glucosidase inhibition, kinetics, and molecular docking studies on isolated diastereomeric alkaloids from Uncaria longiflora by Nurul Aina Suzlin Sulaiman, Muhammad Farhan Syakir Nor Azman, Mohd Fadhlizil Fasihi Mohd Aluwi, Zainul Amiruddin Zakaria, Mohammad Jemain Mohammad Ridhwana, Fatimah Salim

“…Although the high concentrations required for effective enzyme inhibition may limit the practical application of these compounds as antidiabetic therapies, this study provides valuable insights into the chemistry underlying the plant’s previously reported antidiabetic potential, particularly in a stereospecific context. …”
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Isolation of competent actinomycetes strains from soil planted with three species of mint irrigated with olive mill wastewater by Oumaima Beroigui, Lahsen El Ghadraoui, Faouzi Errachidi

“…The application of 5 L of OMWW per m² is optimal for maximum hydrolytic enzyme production by actinomycetes. Dimensional analysis (principal component analysis) is the best method for studying soil-microorganism-plant associations.…”
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