EFFICACY OF THIONONTRITE-S FOR PREVENTION OF METABOLIC LIVER DISEASE IN CATTLE by Ye. V. Tyapkina, M. P. Semenenko, Ye. V.  Kuzminova, A. A.  Abramov

“…The preparation restored functional and barrier properties of the liver, normalized the enzyme-forming function. At the end of the experiment the level of alanine aminotransferase in the first group was 1.8 times less than the control levels, the level of aspartate aminotransferase was 1.6 times lower than the negative control levels. …”
Get full text

Physicochemical properties and biological activities of polysaccharides from the peel of Dioscorea opposita Thunb. extracted by four different methods by Zuowei Zhao, Li Wang, Yuan Ruan, Chunnan Wen, Menghuan Ge, Yanyan Qian, Bingji Ma

“…The influences of 4 extraction methods, namely hot water extraction (W), acidic extraction (HA), hot-compressed water extraction (HCW) and enzyme-assisted extraction (EAE), on the yields, physicochemical properties, hypoglycemic and antioxidant activities of polysaccharides from DTTP were studied and compared. …”
Get full text

Protein Disulfide Isomerase and Host-Pathogen Interaction by Beatriz S. Stolf, Ioannis Smyrnias, Lucia R. Lopes, Alcione Vendramin, Hiro Goto, Francisco R. M. Laurindo, Ajay M. Shah, Celio X. C. Santos

“…., intra- and interdisulfide and cysteine oxidation) and (ii) phagocytic ROS production via Nox family NADPH oxidase enzyme and the control of phagolysosome function with key implications for antigen processing. …”
Get full text

Characterization and ameliorative effects of Chenopodium murale hydroethanolic extract against diethylnitrosamine-induced hepato-renal damage and malfunction by Howida S. Abou- Seif, Osama M. Ahmed, Samah A. El-Newary, Marwa E. Shabana, Mahitab I. EL-Kassaby, Ghada M. Elqattan, Hussein G. Sawie

“…Results DEN has injurious effects, associated with elevated liver enzyme activities (AST, ALT and ALP), urea and creatinine in serum. …”
Get full text

Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis by Jinxia Yu, Yane Gao, Hongsheng Bi, Youhua Zhang, Kai Tang, Dadong Guo, Xiaofeng Xie

“…Finally, the pathogens identified by MGS were verified using both enzyme-linked immune sorbent assay (ELISA) and quantitative PCR (qPCR). …”
Get full text

A cell-based Papain-like Protease (PLpro) activity assay for rapid detection of active SARS-CoV-2 infections and antivirals. by Anahi G Jimenez-Campos, Lucas I Maestas, Nileena Velappan, Brian Beck, Chunyan Ye, Keith Wernsing, Yaniksa Mata-Solis, William J Bruno, Silas C Bussmann, Steven Bradfute, Justin T Baca, Frauke H Rininsland

“…Here we introduce a simple and rapid activity-based diagnostic (ABDx) test that identifies SARS-CoV-2 infections by measuring the activity of a viral enzyme, Papain-Like protease (PLpro). The test system consists of a peptide that fluoresces when cleaved by SARS PLpro that is active in crude, unprocessed lysates from human tongue scrapes and saliva. …”
Get full text

Effect of Rho-Kinase and Autophagy on Remote Ischemic Conditioning-Induced Cardioprotection in Rat Myocardial Ischemia/Reperfusion Injury Model by Jie Gao, Feng Min, Shasha Wang, Heng Lv, Huan Liang, Ben Cai, Xianjie Jia, Qin Gao, Ying Yu

“…Histopathology and ultrastructure and myocardial enzymes’ expression were evaluated to determine the degree of cardiac injury. …”
Get full text

Enzymatic Modification of Apple Pomace and Its Application in Conjunction with Probiotics for Jelly Candy Production by Jolita Jagelavičiutė, Dalia Čižeikienė, Loreta Bašinskienė

“…Enzymatically modified AP had different carbohydrate compositions and technological properties, depending on the enzyme preparation used. Although the viability of probiotics in the jelly candies decreased during storage, a significantly higher viability of <i>B. animalis</i> was determined in jelly candies supplemented with hydrolysed AP compared with control candies made without AP after digestion in the saline, gastric, and intestine phases. …”
Get full text

Bile cast nephropathy (cholemic nephropathy) associated with hepatitis A-induced acute liver failure and haemolysis in a patient with glucose-6-phosphate dehydrogenase deficiency by Ali Hamdan, Johny Salem, Karam Karam, Maria Ziadeh, Pierre Hani

“…Introduction: The hepatitis A virus (HAV) is a common cause of acute hepatitis, while glucose-6-phosphate dehydrogenase (G6PD) deficiency is a widespread enzyme disorder that predisposes individuals to haemolysis and hyperbilirubinemia. …”
Get full text

Suppression of Aldosterone Synthesis and Secretion by Channel Antagonists by Keiichi Ikeda, Tsuyoshi Isaka, Kouki Fujioka, Yoshinobu Manome, Katsuyoshi Tojo

“…Hypertension can be generally treated by negating the effects of angiotensin II through the use of angiotensin-converting enzyme inhibitors (ACE-Is) or angiotensin II type 1 receptor antagonists (ARBs). …”
Get full text

Frequency of 25-Hydroxyvitamin D Deficiency in Pediatric Patients with Immune Thrombocytopenia: Disease Phase and Therapy Options by Huda K. Abbas, Basil O. Saleh, Hassanain H. Ghali

“…The serum 25-hydroxyvitamin D level was measured using the enzyme-linked immunosorbent assay (ELISA) technique. …”
Get full text

Inflammation and ER Stress Downregulate BDH2 Expression and Dysregulate Intracellular Iron in Macrophages by Susu M. Zughaier, Brandon B. Stauffer, Nael A. McCarty

“…We also show that ER stress combined with inflammation synergistically upregulated the expression of the iron carrier protein NGAL and the stress-inducible heme degrading enzyme heme oxygenase-1 (HO-1) leading to iron liberation. …”
Get full text

Transcriptome sequencing reveals jasmonate playing a key role in ALA-induced osmotic stress tolerance in strawberry by Yan Zhong, Xin Wei, Jianting Zhang, Liangju Wang

“…The findings indicated that ALA enhanced osmotic stress tolerance reflected by enhancing relative water content (RWC), root development, gas exchange parameters and antioxidant enzyme activities, and decreasing the leaf H2O2 and malondialdehyde (MDA) content. …”
Get full text

Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ by HAO Lanlan, WANG Hong, ZHANG Fan, ZHANG Xuebing, YANG Yang

“…In addition, overexpression of this gene in A . thaliana increased root length and enzyme activity, suggesting a similar function in A . thaliana. …”
Get full text

The Role of E-Cadherin in the Assessment of Esophageal Mucosal Damage in Patients with Gastroesophageal Reflux Disease Associated with Obstructive Sleep Apnea/Hypopnea Syndrome by Y. Ya. Shaukovich

“…The concentration of E-cadherin in the blood plasma was determined using enzyme immunoassay. Results. There were no statistically significant differences in the plasma level of the N-terminal E-cadherin fragment between GERD patients and the comparison group (0.207 (0.128; 0.295) and 0.128 (0.067; 0.281) ng/ml, respectively, p = 0.082). …”
Get full text

Identification of B Cell Subpopulations with Pro- and Anti-Tumorigenic Properties in an Immunocompetent Mouse Model of Head and Neck Squamous Cell Carcinoma by Michael Sonntag, Sandra Stanojevic, Simon Laban, Patrick J. Schuler, Thomas K. Hoffmann, Cornelia Brunner

“…Additionally, immunoglobulin (Ig) levels post-tumor induction were tracked via enzyme-linked immunosorbent assays (ELISA). Following tumor induction, GCs, as well as increasing numbers of GL7⁺CD95⁺ GC B cells in the spleen and tumor tissues, were detected. …”
Get full text

Potential of Inducible Nitric Oxide Synthase as a Therapeutic Target for Allergen-Induced Airway Hyperresponsiveness: A Critical Connection to Nitric Oxide Levels and PARP Activity by Salome’ V. Ibba, Mohamed A. Ghonim, Kusma Pyakurel, Matthew R. Lammi, Anil Mishra, A. Hamid Boulares

“…This dichotomy calls for better strategies with which the enzyme is adequately targeted. Here, we confirm iNOS expression in the asthmatic lung with concomitant protein nitration and poly(ADP-ribose) polymerase (PARP) activation. …”
Get full text

Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 by Patricia Méndez-Samperio, Elena Miranda, Abraham Vázquez

“…The levels of immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked immunosorbent assay. We found that, although M. bovis BCG induced gene expression of CXCL-8 and CXCL-10 in M. bovis BCG-infected human epithelial cells, CXCL-8 mRNA level was significantly reduced by IL-4, whereas no significant effect of IL-4 was observed on CXCL10 mRNA level. …”
Get full text

Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants by Samia Hamane, Anass El Yemlahi, Mounir Hassani Zerrouk, Ouiam El Galiou, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

“…The screening for lytic enzyme production revealed that all strains were capable of producing chitinase, cellulase, catalase, and protease, while the secretion of amylase and urease was not detected. …”
Get full text

Formulation and development of Serratiopeptidase enteric coated tablets and analytical method validation by UV Spectroscopy by Vijay Kumar Panthi, Saurav Kumar Jha, Raghvendra Chaubey, Rudra Pangeni

“…Serratiopeptidase (SRP) is a proteolytic enzyme that emerged as one of the most potent anti-inflammatory and analgesic drugs. …”
Get full text