Serum Levels of LL-37 and Inflammatory Cytokines in Plaque and Guttate Psoriasis by Young Ji Hwang, Ho Jung Jung, Min Jung Kim, Nam Kyung Roh, Jae Wook Jung, Yang Won Lee, Yong Beom Choe, Kyu Joong Ahn

“…A multiplex cytokine assay and enzyme-linked immunosorbent assay were used to measure levels of Th1- and Th17-derived cytokines and LL-37, respectively. …”
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Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana by Sayo Kashiwagi, Isao Kuraoka, Yoshie Fujiwara, Kenichi Hitomi, Quen J. Cheng, Jill O. Fuss, David S. Shin, Chikahide Masutani, John A. Tainer, Fumio Hanaoka, Shigenori Iwai

“…Moreover, the activity of this enzyme was retained in the presence of a higher concentration of organic solvents. …”
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Multimodal low-opioid anesthesia as a factor in reducing the activation of the proinflammatory response in cardiac surgery by S.R. Maruniak, O.A. Loskutov, O.M. Druzhyna, I.R. Malysh, V.G. Kolesnikov, N.O. Korotchuk

“…The determination of the level of interleukin-6 in the blood was carried out before and after the completion of cardiopulmonary bypass by enzyme-linked immunosorbent assay. Results. Conducting a low opioid multimodal and standard anesthetic management scheme was characterized by a relatively similar effect on the dynamics of systolic blood pressure and heart rate during CABG with cardiopulmonary bypass, except intubation stage, at which these indicators were significantly lower by 16.21 % (p = 0.001) and by 15.45 % (p = 0.001), respectively, in patients with a low opioid anesthetic management scheme. …”
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Podocyturia: A Clue for the Rational Use of Amiloride in Alport Renal Disease by H. Trimarchi, R. Canzonieri, A. Muryan, A. Schiel, A. Araoz, M. Paulero, J. Andrews, T. Rengel, M. Forrester, F. Lombi, V. Pomeranz, R. Iriarte, E. Zotta

“…Commonly drug-based approaches include angiotensin-converting enzyme inhibitors and angiotensin receptor blockers, which are employed to reduce proteinuria and thus retard kidney disease progression and cardiovascular morbidity and mortality. …”
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The m6A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells by Qian Gao, Jin Zheng, Zegui Ni, Pengli Sun, Congcong Yang, Maosheng Cheng, Mingqing Wu, Xiuhong Zhang, Lin Yuan, Yingyin Zhang, Yang Li

“…Here, we show that global RNA m6A abundance and the expression of m6A-forming enzyme METTL3 are higher in BCSCs than those in non-CSCs of bladder cancer (BCa) cells. …”
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Extracellular Vesicles Released from Neprilysin Gene-Modified Human Umbilical Cord-Derived Mesenchymal Stem Cell Enhance Therapeutic Effects in an Alzheimer’s Disease Animal Model... by HyeJu Jeong, Ok Joon Kim, Seung-Hun Oh, Sanghoon Lee, Han A. Reum Lee, Kee Ook Lee, Boo-Yong Lee, Nam Keun Kim

“…Neprilysin (NEP) is the main cleavage enzyme of β-amyloid (Aβ), which plays a major role in the pathology and etiology of AD. …”
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The triterpenoids-enriched extracts from Antrodia cinnamomea mycelia attenuate alcohol-induced chronic liver injury via suppression lipid accumulation in C57BL/6 mice by Yange Liu, Ronglong Chen, Lanzhou Li, Ruitao Dong, Hui Yin, Yawen Wang, Anhui Yang, Jianbin Wang, Changtian Li, Di Wang

“…Based on proteomic analysis and enzyme-linked immunosorbent assay, ACT had been confirmed to regulate the levels of lipid biogeneration-related factors and depressed the over-accumulation of hepatic reactive oxygen species. …”
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Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKKβ-AMPK-SIRT1 Signaling Pathway by Xin Liu, Ning Wang, Yuanfeng Zhu, Yongjun Yang, Xiaoli Chen, Shijun Fan, Qian Chen, Hong Zhou, Jiang Zheng

“…AMPK activation increased the NAD+/NADH ratio and activated Sirtuin 1 (SIRT1), a NAD+-dependent deacetylating enzyme and negative regulator of inflammation. Chemical inhibitor or siRNA of SIRT1 enhanced IL-12 release while its agonist suppressed IL-12 production. …”
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Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Contr... by Laís de Souza Gouveia Moreira, Karla Thaís Resende Teixeira, Ludmila F. M. F. Cardozo, Livia Alvarenga, Bruna Regis, Jessyca Sousa de Brito, Viviane de Oliveira Leal, Natalia Alvarenga Borges, Isabela de Souza da Costa Brum, José Carlos Carraro-Eduardo, Giovanna B. Borini, Andresa A. Berretta, Marcelo Ribeiro-Alves, Denise Mafra

“…Interleukin-6 (IL-6) plasma levels were assessed by enzyme-linked immunosorbent assay and C-reactive protein (CRP) by immunoturbidimetric method. …”
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H. Wendl Leaf Metabolites Potentiate the Radiosensitivity of Hepatocellular Carcinoma Through Ki67 and PARP Inhibition by Nabil M. Selim PhD, Seham S. El-Hawary PhD, Soheir M. El Zalabani PhD, Rehab Nabil Shamma PhD, Nariman El Sayed Mahdy PhD, Noheir H. Sherif PhD, Khayrya A. Youssif PhD, Usama Ramadan Abdelmohsen PhD, Mai H. Mekkawy PhD, Hanan A. Fahmy PhD

“…In addition, NE treatment before IRR significantly decreased the expression of the poly ADP ribose polymerase-1 ( PARP1) enzyme and Ki67. Furthermore, the histological investigations strongly confirmed the combined effect of NE and IRR in fighting DEN-induced HCC. …”
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Enzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products by Maria Sapatinha, Carolina Camacho, Antónia Juliana Pais-Costa, Ana Luísa Fernando, António Marques, Carla Pires

“…Additionally, AP hydrolysates demonstrated over 60% angiotensin-converting enzyme (ACE) inhibition at 5 mg/mL, indicating potential antihypertensive applications. …”
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Assembly of soil multitrophic community regulates multifunctionality via multifaceted biotic factors in subtropical ecosystems by Chao Chang, En Hu, Xiaofeng Tang, Sisi Ye, Dan Zhao, Zhi Qu, Ming Li

“…High-throughput sequencing was used to examine archaea, bacteria, fungi, algae, protozoa, and invertebrates, while enzyme activity assays were used to assess ecosystem multifunctionality related to nutrient provisioning. …”
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The Lyn/RUVBL1 Complex Promotes Colorectal Cancer Liver Metastasis by Regulating Arachidonic Acid Metabolism Through Chromatin Remodeling by Zhenyu Zhang, Yina Gao, Yuanyuan Qian, Bowen Wei, Kexin Jiang, Zhiwei Sun, Feifan Zhang, Mingming Yang, Salem Baldi, Xiaoqi Yu, Yunfei Zuo, Shuangyi Ren

“…On the other hand, metabolomics revealed that Lyn/RUVBL1 regulates the expression of PGE2 through the enzyme COX2, thereby promoting arachidonic acid (AA) metabolism. …”
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Effectively alleviate rheumatoid arthritis via maintaining redox balance, inducing macrophage repolarization and restoring homeostasis of fibroblast-like synoviocytes by metformin-... by Rui Zhang, Xingyu Lin, Rongjie Lin, Zhenbin Chen, Chenfang Miao, Yao Wang, Xiaoqin Deng, Jianlong Lin, Shishui Lin, Shaohuang Weng, Min Chen

“…In addition, MCDs eliminated the cellular ROS in M1 phenotype macrophages in RA microenvironment through the enzyme-like catalytic activity as well as inhibiting NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome signaling pathway, effectively polarizing them into the M2 phenotype to realize the anti-inflammatory effect. …”
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Butyric Acid Modulates Gut Microbiota to Alleviate Inflammation and Secondary Bone Loss in Ankylosing Spondylitis by Ke You, Lianjun Yang, Zhihai Su, Jun Shen, Xinyang Fan, Yuanqing Guo, Zhen Yuan, Hai Lu

“…Various techniques, including an enzyme-linked immunosorbent assay (ELISA), magnetic resonance imaging (MRI), micro-CT, histology, quantitative PCR (qPCR) for intestinal tight junction protein expression, and 16S rDNA sequencing to analyze gut microbiota abundance, were employed to assess the inflammation and bone health in the target tissues. …”
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Bushen Huoxue formula protects against renal fibrosis and pyroptosis in chronic kidney disease by inhibiting ROS/NLRP3-mediated inflammasome activation by Lin Liao, Pengyu Tao, Qiming Xu, Jie Chen, Weiwei Liu, Jing Hu, Jianrao Lu

“…Related markers of fibrosis and NOD-like receptor protein 3 (NLRP3) inflammasome activation were measured using western blotting and enzyme-linked immunosorbent assay. Treatment with BSHX (0.25, 0.5, and 1 g/kg) significantly inhibited renal fibrosis and damage in 5/6 nephrectomized rats and simultaneously reduced oxidative stress and NLRP3 inflammasome activation. …”
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Newcastle disease virus genotype VII.1.1 identified from backyard chickens with low antibody titer: Jimma Zone, Southwest Ethiopia. by Bezina Arega Emeru, Haregawi Tesfaye Desta, Yosef Deneke

“…Samples were collected from Jimma town, Seqa Chekorsa and Tiro Afeta districts of Jimma zone. Enzyme linked immunosorbent assay (ELISA) was conducted and seropositivity was determined from the collected serum samples. …”
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The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway by Jiaying Huo, Yuezhang Ming, Huifeng Li, Anjun Li, Jiwen Zhao, Mingquan Huang, Weizheng Sun, Jihong Wu, Jinglin Zhang

“…The results showed that these peptides exerted protective effects by suppressing reactive oxygen species (ROS) generation, preventing malondialdehyde (MDA) formation, and upregulating cellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) in a dose-dependent manner. …”
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Tolterodine is a novel candidate for assessing CYP3A4 activity through metabolic volatiles to predict drug responses by Valentina Stock, Rebecca Hofer, Franziska Lochmann, Vera Spanke, Klaus R. Liedl, Jakob Troppmair, Thierry Langer, Hubert Gstach, Christian Dank, Chris A. Mayhew, Sarah Kammerer, Veronika Ruzsanyi

“…These findings pave the way for the further development of non-invasive breath tests using unlabelled precursors to determine CYP enzyme activity in individuals.…”
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The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Interv... by Jun Jia, Shan-zheng Wang, Liang-yu Ma, Jia-bin Yu, Yu-dong Guo, Chen Wang

“…NPMSCs isolated from rabbit NP tissues were treated with L-PRP or P-PRP in vitro, and then cell proliferation and expression of stem cell markers, proinflammatory cytokines (TNF-α, IL-1β), production of ECM (extracellular matrix-related protein), and NF-κB p65 protein were validated by CCK-8 assay, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and western blot respectively. …”
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