Exploring diazotrophic diversity: unveiling Nif core distribution and evolutionary patterns in nitrogen-fixing organisms by Bruno Thiago de Lima Nichio, Roxana Beatriz Ribeiro Chaves, Fábio de Oliveira Pedrosa, Roberto Tadeu Raittz

“…Abstract Background Diazotrophs carry out biological nitrogen fixation (BNF) using the nitrogenase enzyme complex (NEC), which relies on nitrogenase encoded by nif genes. …”
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Essential Oils from Citrus Peels Promote Calcium Overload-Induced Calcicoptosis in U251 Cells by Yurong Li, Juanjuan Wei, Zimao Ye, Chen Ji, Wenji Li, Li Xu, Zhiqin Zhou

“…Additionally, the antioxidant enzyme system (SOD, POD, CAT, and GR) and the nonenzymatic defense system (GSH) were inhibited, leading to an increase in lipid peroxidation. qRT–PCR indicated the significant upregulation of intracellular calcium ion signaling pathways and the upregulation of mitochondrial apoptosis-related genes. …”
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Tetramycin ameliorates tebuconazole·azoxystrobin to control leaf spot and viral diseases of Taizishen by Bing Tian, Bing Tian, Chenglin Tang, Jiaqi Liu, Boya Jin, Cheng Zhang

“…Concurrently, their combined application could notably enhance total soluble flavonoids, total soluble phenols, protective enzyme activity, IC, intracellular water metabolism, nutrient transport, and metabolic activity, while reducing its MDA, IR, IZ, IXc, and IXL. …”
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Anti-motilities and anti-biofilm effects of Ageratum conyzoides L. methanol extract by Eli Compaoré, Moussa Compaoré, Vincent Ouédraogo, Ablassé Rouamba, Alimata Bancé, Mignini R. Dofini, Martin Kiendrebeogo

“…The anti-biofilm effect was conducted in microtiter plates using the crystal violet method. The antioxidant and enzyme inhibition activities were evaluated using 2,2-diphényl-1-picrylhydrazyl; 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulfonic acid), Ferric Reducing Antioxidant Power and conducting lipoxygenase test. …”
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Association of fatty acid desaturase 2 gene polymorphism (rs28456) with susceptibility to bipolar disorder in the Turkish population: A case-control study by Pirim Dilek, Gümüş Ceren, Boztepe Esra, Akdağ Emine Merve, Şirin Hande

“…The FADS2 gene encodes a key, rate-limiting enzyme involved in polyunsaturated fatty acid (PUFA) metabolism. …”
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Carvacrol acetate activated Nrf2 modulates mitophagy for the treatment of neurocyte oxidative stress induced by chlorpyrifos by Hong-Ling Zhou, Bei-Bei Wang, Xu-Li Fan, Xiao-Min Zhang, Ying Song

“…Furthermore, ROS and MDA levels were not significantly reduced, SOD enzyme activity did not increase, and mitochondrial membrane potential and mtDNA copy number did not improve. …”
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Effects of Dietary Vitamin C on the Growth Performance, Biochemical Parameters, and Antioxidant Activity of Coho Salmon Oncorhynchus kisutch (Walbaum, 1792) Postsmolts by Cong-mei Xu, Hai-rui Yu, Ling-yao Li, Min Li, Xiang-yi Qiu, Xiao-qian Fan, Yan-lin Fan, Ling-ling Shan

“…The dietary VC requirement was in the range of 93.08–224.68 mg/kg for optimum growth performance, serum enzyme activities, and antioxidant capacity of coho salmon postsmolts.…”
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Supplementation with active vitamin D3 ameliorates experimental autoimmune thyroiditis in mice by modulating the differentiation and functionality of intrathyroidal T-cell subsets by Chun-Mei Wang, Ying-Jie Chen, Bo-Cheng Yang, Jia-Wen Yang, Wei Wang, Yang Zeng, Jun Jiang

“…After 8 weeks, the morphological architecture of the mouse thyroid follicles was examined by histological sections, thyroid autoantibodies and thyroid hormone concentrations were determined by enzyme-linked immunosorbent assays (ELISAs), and the major functions and subsets of B- and T-lymphocytes in the mouse thyroid were determined by tissue multiple immunofluorescence technology and ELISA.ResultsEAT caused thyroiditis follicle destruction and interfollicular lymphocyte infiltration in mice, increased concentrations of circulating thyroid autoimmune antibodies TG-Ab and TPO-Ab, and abnormal thyroid hormone levels. …”
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MATN3 Gene Polymorphism Is Associated with Osteoarthritis in Chinese Han Population: A Community-Based Case-Control Study by Jiaao Gu, Jiesheng Rong, Fulin Guan, Liying Jiang, Shuqing Tao, Guofa Guan, Tianzun Tao

“…After obtaining the DNA of case and control groups, genotypes of the MATN3 SNP6 were determined by polymerase chain reaction followed by restriction enzyme digestion. The numbers of patients with different OA subtypes were also calculated. …”
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Cerebral hypoperfusion reduces tau accumulation by Ghupurjan Gheni, Mitsuru Shinohara, Masami Masuda‐Suzukake, Akihiko Shindo, Atsushi Watanabe, Kaori Kawai, Guojun Bu, Hidekazu Tomimoto, Masato Hasegawa, Naoyuki Sato

“…Additionally, cerebral hypoperfusion increased a lysosomal enzyme, cathepsin D. Interpretation These data together indicate that cerebral hypoperfusion reduces tau accumulation likely through an increase in microglial phagocytic activity towards tau and an elevation in degradation through cathepsin D. …”
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Protective Effect of High-Intensity Interval Training (HIIT) and Moderate-Intensity Continuous Training (MICT) against Vascular Dysfunction in Hyperglycemic Rats by Nurul Paramita, Brilliant C. Puspasari, Randika Arrody, Neng T. Kartinah, Trinovita Andraini, Julfiana Mardatillah, Hardiyanti Rusli, Dewi I. S. Santoso

“…The ascending aorta (until the abdominal artery) was examined. An enzyme-linked immunosorbent assay (ELISA) was used to measure the glucagon-likepeptide-1 (GLP-1), endothelial nitric oxide synthase (eNOS), and tumor necrosis factor-alpha (TNFα) levels. …”
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Halothermophilic Cas9 and dCas9 Interaction with Variety sgRNA In-silico by Kenny Lischer, Kholisoh Hayati, Muhammad Haykal Fabian, Fina Amreta Laksmi, Yudhi Nugraha, Hamdan Dwi Rizqi, Riri Fauziyya, Sarmoko, Mohd Shukuri Mohamad Ali

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Change of Vascular Endothelial Growth Factor Levels following Vitrectomy in Eyes with Proliferative Diabetic Retinopathy by Hui-Jin Chen, Zhi-Zhong Ma, Ying Li, Chang-Guan Wang

“…VEGF concentrations in the samples were determined by enzyme-linked immunosorbent assay. Recurrent vitreous hemorrhage and neovascular glaucoma within six months postvitrectomy were also analyzed. …”
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Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model by Toshio Miki, Ludivina Vazquez, Lisa Yanuaria, Omar Lopez, Irving M. Garcia, Kazuo Ohashi, Natalie S. Rodriguez

“…Mucopolysaccharidosis type 1 (MPS-1), also known as Hurler’s disease, is a congenital metabolic disorder caused by a mutation in the alpha-L-iduronidase (IDUA) gene, which results in the loss of lysosomal enzyme function for the degradation of glycosaminoglycans. …”
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Screening of two efficient phosphorus-solubilizing bacteria, strain X42 (Bacillus_A_bombysepticus) and strain G62 (Bacillus velezensis) for their ability to promote Gan Nan navel o... by Wenyuan Zhao, Tao Peng, Haojie Cao, Huimin Huang, Shuijing Yu

“…A comparison of the growth indicators of the experimental and control groups, as well as the enzyme activity indicators of navel orange leaves, revealed that both strains exhibited good growth-promoting effects. …”
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Prognostic importance of human leukocyte antigen DRβ1 gene and protein tyrosine phosphatase nonreceptor Type 22 gene polymorphism in rheumatoid arthritis by Pramod Kumar Verma, Usha Singh, Shyam Kumar Saraf, Narendra Kumar Singh

“…HLA DRβ1 typing was done by low-resolution sequence-specific primer polymerase chain reaction and auto-antibodies (Abs) (rheumatoid factor [RF], anti-cyclic citrullinated peptide 2 (CCP2) Ab, anti-nuclear Ab, double-stranded deoxyribose nucleic acid, anticardiolipin Ab) were done by enzyme-linked immunosorbent assay. Results: In patients with RA, the most common HLA DRβ1 allele was DRβ1*04 (23.3%), followed by DRβ1*10 (20.7%), DRβ1*03 (9.3%), and DRβ1*01 (8.7%). …”
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Structure-Based Virtual Screening, Docking, ADMET, Molecular Dynamics, and MM-PBSA Calculations for the Discovery of Potential Natural SARS-CoV-2 Helicase Inhibitors from the Tradi... by Ahmed M. Metwaly, Alaa Elwan, Abdul-Aziz M. M. El-Attar, Sara T. Al-Rashood, Ibrahim H. Eissa

“…Initially, a fingerprint study with VXG, the ligand of the target enzyme, disclosed the similarity of 187 compounds. …”
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Preventive Effect of Salicylate and Pyridoxamine on Diabetic Nephropathy by Tarek Kamal Abouzed, Seiichi Munesue, Ai Harashima, Yusuke Masuo, Yukio Kato, Khaled Khailo, Hiroshi Yamamoto, Yasuhiko Yamamoto

“…Attenuation of macrophage infiltration into the kidneys and upregulation of antiglycating enzyme glyoxalase 1 gene expression were found only in the salicylate treatment group. …”
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Prevalence and Residual Risk of HIV in Volunteer Blood Donors of Zhejiang Province, China, from 2018 to 2022 by Hong Zhu, Wei Ding, Wenjuan Han, Xiaofan Zheng, Yiqing Hu, Jie Dong, Yaling Wu, Danxiao Wu, Jinhui Liu, Faming Zhu

“…The HIV antibody or antigen and HIV RNA were detected using enzyme-linked immunosorbent assay and NAT, respectively. …”
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Endothelial Glycocalyx Shedding Occurs during Ex Vivo Lung Perfusion: A Pilot Study by Timothy M. Sladden, Stephanie Yerkovich, Douglas Wall, Maxine Tan, William Hunt, Jonathan Hill, Ian Smith, Peter Hopkins, Daniel C. Chambers

“…Endothelial glycocalyx products accumulate during both porcine and human EVLP, and this accumulation parallels an accumulation of matrix-degrading enzyme activity. Preliminary evidence in our porcine EVLP model suggests that shedding may be related to organ function, thus warranting additional study.…”
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