Caveolin-1 Knockdown Decreases SMMC7721 Human Hepatocellular Carcinoma Cell Invasiveness by Inhibiting Vascular Endothelial Growth Factor-Induced Angiogenesis by Zhi-Bo Zhang, Zheng Shi, Lan-Fang Yang, Hong-Bin Gao

“…Secreted VEGF levels in SMMC7721 cells were evaluated by enzyme-linked immunosorbent assay (ELISA). SMMC7721 cell proliferation, cycle, apoptosis, and invasiveness were detected by MTT, flow cytometry, Annexin V-FITC/PI, and invasion assay, respectively. …”
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Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway by Ye-Hong Xv, Wei Gao, Liao-Jv Wang

“…Receiver operating characteristic (ROC) analysis was carried out to predict the diagnostic values of UCA1, miR-16 and PAPP-A in PE. Enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (PCR), Western blot analysis, immunohistochemistry (IHC) assay, computational analysis, and luciferase assay were conducted to measure the differential expression of UCA1, miR-16, and PAPP A while establishing a signaling pathway of UCA1/miR-16/PAPP-A. …”
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Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model by Akinjide Moses Akinnuga, Angezwa Siboto, Bongiwe Khumalo, Ntethelelo Hopewell Sibiya, Phikelelani Ngubane, Andile Khathi

“…However, the administration of BA decreased liver enzyme concentrations, decreased hepatic oxidative stress, and improved antioxidant enzymes such as SOD and GPx. …”
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Dietary inclusion of African bush mango (Irvingia gabonensis) seed improves sexual performance in sexually inexperienced rats by Olasunkanmi Omojokun, Akindele Famurewa, Ganiyu Oboh, Jemima Enang, Stella Ijeh

“…The experiment lasted for 21 days after which behavioral studies involving sexual and anxiety indices/measurements, lipid peroxidation, and enzyme assays (arginase and phosphodiesterase-5 [PDE-5]) were conducted. …”
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Exploring Quality of Life in Adults Living With Late-onset Pompe Disease: A Combined Quantitative and Qualitative Analysis of Patient Perceptions from Australia, France, Italy, and... by Holly Lumgair, Lisa Bashorum, Alasdair MacCulloch, Elizabeth Minas, George Timmins, Drago Bratkovic, Richard Perry, Medi Stone, Vasileios Blazos, Elisabetta Conti, Raymond Saich

“…Physical functioning scores were generally stable in respondents who had been receiving enzyme replacement therapy (ERT) for 1-15 years, but those who had received ERT for >15 years had lower scores. …”
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Peptide Design for Enhanced Anti-Melanogenesis: Optimizing Molecular Weight, Polarity, and Cyclization by Putri SA, Maharani R, Maksum IP, Siahaan TJ

“…Tyrosinase (TYR), a key enzyme in the melanogenesis pathway, catalyzes the biosynthesis of melanin in the skin. …”
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Soluble RAGE Plasma Levels in Patients with Coronary Artery Disease and Peripheral Artery Disease by Colomba Falcone, Sara Bozzini, Luigina Guasti, Angela D’Angelo, Anna Clizia Capettini, Edoardo Maria Paganini, Rossana Falcone, Roberto Moia, Carmine Gazzaruso, Gabriele Pelissero

“…Clinical and laboratory parameters including the ankle brachial index (ABI) and sRAGE (enzyme-linked immunosorbent assay kit) were investigated in 544 patients with angiographically documented CAD and 328 control subjects. 213/554 CAD patients (39%) showed an ABI <0.9 associated with typical symptoms (group CAD + PAD), whereas 331 patients were free from PAD. …”
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IgG, IgM, and IgA Antinuclear Antibodies in Discoid and Systemic Lupus Erythematosus Patients by Sheridan A. Jost, Lin-Chiang Tseng, Loderick A. Matthews, Rebecca Vasquez, Song Zhang, Kim B. Yancey, Benjamin F. Chong

“…In this cross-sectional study, sera from age-, gender-, and ethnic-matched SLE N=35, DLE N=23, and normal patients N=22 were tested for IgG, IgM, and IgA ANAs using enzyme-linked immunosorbent assays (ELISAs) and indirect immunofluorescence (IIF) with monkey esophagus as substrate. …”
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Indoxyl Sulfate Induces Mesangial Cell Proliferation via the Induction of COX-2 by Shuzhen Li, Sijie Cheng, Zhenzhen Sun, Harr-keshauve Mungun, Wei Gong, Jing Yu, Weiwei Xia, Yue Zhang, Songming Huang, Aihua Zhang, Zhanjun Jia

“…Next, we found an inducible inflammation-related enzyme COX-2 was remarkably enhanced by IS, and the inhibition of COX-2 by NS398 significantly blocked IS-induced MC proliferation in line with a blockade of PGE2 production. …”
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Clinical Significance of Lymph Node Micrometastasis in pN0 Gastric Cancer Patients by Yu Li, Dongsheng Wang, Yi Li, Xiaodong Liu, Dong Chen, Chentong Yuan, Yanbing Zhou

“…The LNMM was diagnosed by telomeric repeat amplification protocol/enzyme-linked immunosorbent assay (TRAP-ELISA). The relationship between clinicopathological factors and LNMM was investigated by multivariate analysis. …”
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Difference in the Vitreal Protein Profiles of Patients with Proliferative Diabetic Retinopathy with and without Intravitreal Conbercept Injection by Chen Zou, Minjie Zhao, Jingjing Yu, Dandan Zhu, Yunzhi Wang, Xinping She, Yanan Hu, Zhi Zheng

“…Intravitreal levels of apolipoprotein A-II (APOA2) and ceruloplasmin (CP) were measured using enzyme-linked immunosorbent assays. Results. 307 proteins were expressed differentially between PDR and IVC groups, including 218 proteins downregulated in response to IVC. …”
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NT5E (CD73) as a prognostic biomarker and therapeutic target associated with immune infiltration in lung adenocarcinoma by Leyan Chen, Tuoya Qi, Bishu Zhang, Xuelong Wang, Mingfeng Zheng

“…NT5E, an ecto-5’-nucleotidase enzyme, has been implicated in cancer progression, particularly in efferocytosis. …”
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In silico methods for immunogenicity risk assessment and human homology screening for therapeutic antibodies by Aimee E. Mattei, Andres H. Gutierrez, Soorya Seshadri, Jacob Tivin, Matt Ardito, Amy S. Rosenberg, William D. Martin, Anne S. De Groot

“…Although this perspective discusses the in-silico immunogenicity risk assessment for monoclonal antibodies, bi-specifics, multi-specifics, and antibody-drug conjugates, the analysis of additional therapeutic modalities such as enzyme replacement proteins, blood factor proteins, CAR-T, gene therapy products, and peptide drugs is also made available on the ISPRI platform.…”
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Effect of decitabine on PD-L2 methylation in whole blood of iodine-induced autoimmune thyroiditis rats by Baiming Jin, Yanbo Qi, Hong Chao, Xiaolei Yang, Hongjie Li, Siyuan Wan

“…After injecting Dec intraperitoneally into EAT rats, we performed arsenic-cerium catalytic spectrophotometry, pathological hematoxylin and eosin staining, enzyme-linked immunosorbent assay, quantitative methylation-specific polymerase chain reaction (qMSP), and quantitative real-time polymerase chain reaction (qPCR) to determine the relevant indices. …”
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A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways in <i>Komagataella phaffii</i> by Albert Fina, Sílvia Àvila-Cabré, Enrique Vázquez-Pereira, Joan Albiol, Pau Ferrer

“…The introduction of heterologous pathways into microbial cell compartments offers several potential advantages, including increasing enzyme concentrations and reducing competition with native pathways, making this approach attractive for producing complex metabolites like fatty acids and fatty alcohols. …”
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Ameliorative Effect of Hovenia dulcis Fruit Pedicel Polysaccharides on Alcohol-Induced Intestinal Injury in Mice Investigated Using 16S rRNA Sequencing and Metabolomics by ZHANG Yuchao, ZHU Sijie, LIU Liangyu, WANG Jianyun, ZHANG Jiaxin, SU Zexiong, CHEN Jiangyan, LIU Xudong

“…Using high-throughput 16S rRNA gene sequencing technology and metabolomics, we investigated the impacts of HDPs on the gut microbiota and metabolic profile of mice with alcohol-induced intestinal injury. Additionally, enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, real-time quantitative polymerase chain reaction (qPCR), and Western blot were employed to detect the changes in the expression of intestinal inflammatory factors, tight junction proteins, bile acid metabolism-related genes and proteins. …”
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Palm Tocotrienol Activates the Wnt3a/β-Catenin Signaling Pathway, Protecting MC3T3-E1 Osteoblasts from Cellular Damage Caused by Dexamethasone and Promoting Bone Formation by Norfarahin Abdullah Sani, Nur Aqilah Kamaruddin, Ima Nirwana Soelaiman, Kok-Lun Pang, Kok-Yong Chin, Elvy Suhana Mohd Ramli

“…The expression of osteoblastic differentiation markers was measured by an enzyme-linked immunoassay. <b>Results</b>: Enhanced matrix mineralization was observed in the cells treated with 0.5 µg/mL PTT, especially on day 18 (<i>p</i> < 0.05). …”
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Pain in pancreatitis: issue and ways out by A. V. Okhlobystin

“…Drug therapy of pancreatic pain is based on digestive enzymes, antisecretory and analgetic agents.…”
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Growth, Feed Utilization, Lipid Metabolism, and Metamorphosis of Bullfrog (Aquarana catesbeianus) Tadpoles Fed Diets With Different Lipid Levels by Juan Gao, Ling Wang, Jian Zhang, Kangle Lu, Kai Song, Xueshan Li, Chunxiao Zhang

“…This study investigated the effect of dietary lipid levels on growth performance, lipid metabolism, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog (Aquarana catesbeianus) tadpoles. …”
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Mold Alkaloid Cytochalasin D Modifies the Morphology and Secretion of fMLP-, LPS-, or PMA-Stimulated Neutrophils upon Adhesion to Fibronectin by Svetlana I. Galkina, Natalia V. Fedorova, Marina V. Serebryakova, Evgenii A. Arifulin, Vladimir I. Stadnichuk, Ludmila A. Baratova, Galina F. Sud’ina

“…Simultaneous detection of neutrophil secretory products by mass spectrometry showed that all tested stimuli caused the secretion of MMP-9, a key enzyme in the neutrophil migration. Cytochalasin D impaired the MMP-9 secretion but initiated the release of cathepsin G and other granular bactericides, proinflammatory agents. …”
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