Genome Mining and Characterization of Two Novel <i>Lacticaseibacillus rhamnosus</i> Probiotic Candidates with Bile Salt Hydrolase Activity by Gianluigi Agolino, Marianna Cristofolini, Amanda Vaccalluzzo, Davide Tagliazucchi, Alice Cattivelli, Alessandra Pino, Cinzia Caggia, Lisa Solieri, Cinzia Lucia Randazzo

“…Bile salt hydrolase (BSH; EC 3.5.1.24) is the microbial enzyme that catalyzes the conversion of primary bile acids (BAs) into secondary ones, promoting microbial adaptation and modulating several host’s biological functions. …”
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Sleep quality in children with hepatic glycogen storage diseases, a prospective observational pilot study by Lucas Agnoletto, Moya Vandeleur, Mary White, Anne‐Marie Adams, Rebecca Halligan, Heidi Peters

“…Abstract Background Hepatic glycogen storage diseases (GSDs) are characterised by enzyme defects affecting liver glycogen metabolism, where carbohydrate supplementation to prevent overnight hypoglycaemia is common. …”
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Changes in Serum Cytokines May Predict Therapeutic Efficacy of Tofacitinib in Rheumatoid Arthritis by Yuxuan Li, Lin Yuan, Jie Yang, Yue Lei, Hui Zhang, Liping Xia, Hui Shen, Jing Lu

“…Serum cytokines levels of Th1 (IFN-γ), Th2 (IL-6), Th17 (IL-17), Tregs (IL-35), and TNF-α were detected by enzyme-linked immunosorbent assays. Results. Disease activity was significantly decreased as early as week 4 after tofacitinib treatment. …”
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Calcium Channel Blocker Lacidipine Promotes Antitumor Immunity by Reprogramming Tryptophan Metabolism by Yuwen Sheng, Chong Qiao, Zhonghui Zhang, Xiaoke Shi, Linhan Yang, Ruiying Xi, Jialing Yu, Wanli Liu, Guolin Zhang, Fei Wang

“…Here, calcium channel blocker lacidipine is identified to potently inhibit the enzymatic activity and expression of indoleamine 2,3‐dioxygenase 1 (IDO1), a rate‐limiting enzyme in tryptophan metabolism. Lacidipine activates effector T cells and incapacitates regulatory T cells (Tregs) to augment the anti‐tumor effect of chemotherapeutic agents in breast cancer by converting immunologically “cold” into “hot” tumors. …”
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Plasmid-free production of the plant lignan pinoresinol in growing Escherichia coli cells by U. Joost Luelf, Alexander Wassing, Lisa M. Böhmer, Vlada B. Urlacher

“…In addition, the simultaneous expression of multiple heterologous genes from different plasmids for a multi-enzyme cascade can be challenging and limits large-scale use. …”
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Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability by Jun HAN, Yi WANG, Kaiping ZHANG, Jianjun TIAN

“…Five sterol esterase-producing enzyme (EC 3.1.1.13) genes, including gene0325 and gene1042, were identified in the metabolic system, which could be hydrolysed to produce sterols and fatty acids. …”
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Impact of dapagliflozin on metabolic phenotype, hormone levels, and fertility in female mice after prolonged high-fat diet by Xiaolin Chen, Zhuoni Xiao, Na Dai, Mingxia Fan

“…Serum hormone concentrations and inflammatory factors in mice aged 28 weeks or 38 weeks were quantified using ultrasensitive enzyme-linked immunosorbent assays (ELISAs). Metabolic parameters were also assessed and documented at different stages of the experiment. …”
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Recent advances in the extraction technology and bioactivity of lentinan by Sizhu Ren, Chunyan Xie, Hua Liu, Liyan Li, Xiaoling Han, Wenjie Chen

“…Many advanced technologies have been applied for lentinan extraction, such as ultrasonic-assisted, microwave-assisted, and enzyme-assisted extractions. However, their advantages and disadvantages differ, which directly affect the structural and functional properties of lentinan. …”
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Administration of PDE4 Inhibitors Suppressed the Pannus-Like Inflammation by Inhibition of Cytokine Production by Macrophages and Synovial Fibroblast Proliferation by Katsuya Kobayashi, Toshio Suda, Haruhiko Manabe, Ichiro Miki

“…Both PDE4 inhibitors, KF66490 and SB207499, suppressed the production of IL-1β, TNF-α, and IL-12, and the increase in myeloperoxidase activity, a marker enzyme for neutrophils and hydroxyproline content. …”
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Correlation between Serum IL-35 Levels and Bone Loss in Postmenopausal Women with Rheumatoid Arthritis by Yuxuan Li, Lutian Yao, Siyan Liu, JishengWu, Liping Xia, Hui Shen, Jing Lu

“…Serum IL-35 levels were detected by enzyme-linked immunosorbent assay. Bone mineral density (BMD) at lumbar spine 1-4 and at total hip was measured using dual-energy X-ray absorptiometry. …”
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Application Effect of Transparent Supervision Based on Informatization in Prevention and Control of Carbapenem-Resistant Klebsiella pneumoniae Nosocomial Infection by Xiaoqin Wu, Quansheng Lu, Guo Feng, Hongxia Kan, Liran Shi

“…Incidence and detection rate of CRKP, utilization rate of special grade hydrocarbon enzyme alkene antibiotics, hand hygiene compliance rate, qualified rate of ATP tests on surface of environmental objects, and execution rate of CRKP core prevention and control were compared between the two groups. …”
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Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota by Kai Kang, Yue Sun, Dan Pan, Bing Chang, Li-Xuan Sang

“…NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores. Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment. …”
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Diagnostic potential of urinary CX3CL1 for amnestic mild cognitive impairment and Alzheimer’s disease by Yali Xu, Yali Xu, Jie Zhang, Jie Zhang, Ying-Ying Shen, Wei-Wei Li, Bin Li, Hai-Ping Cheng, Gui-Hua Zeng

“…This study aims to evaluate the diagnostic potential of urinary CX3CL1 levels in distinguishing between AD patients, those experiencing amnestic mild cognitive impairment (aMCI), and cognitively normal (CN) individuals.MethodsA cohort comprising 516 CN individuals across various age groups, 102 AD patients, and 65 subjects with aMCI was assembled, alongside 93 age- and sex-matched CN controls. Enzyme-linked immunosorbent assay (ELISA) was utilized to quantify urinary CX3CL1 levels.ResultsUrinary CX3CL1 concentrations exhibited an age-dependent increase and demonstrated a positive correlation with age. …”
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The Effect of Mesenchymal Stem Cell-Derived Exosomes and miR17-5p Inhibitor on Multicellular Liver Fibrosis Microtissues by Farnaz Sani, Mina Soufi Zomorrod, Negar Azarpira, Masoud Soleimani

“…The results of cell viability, reactive oxygen species (ROS) production, liver enzyme levels, inflammation, and histopathology were analyzed to assess the treatment efficacy. …”
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Distinct roles of Constitutive Photomorphogenesis Protein 1 homolog (COP1) in human hepatocyte models by Sébastien Soubeyrand, Paulina Lau, Ruth McPherson, Ruth McPherson

“…Cellular impacts were examined by proliferation assays, qRT-PCR, western blotting, reporter assays, and APOB enzyme-linked immunosorbent assays.Results and DiscussionCOP1 suppression had no noticeable impact on HepG2 and HuH-7 proliferation and was associated with contrasting rather than congruent transcriptome changes. …”
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Prevalence and Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolated in Canadian Hospitals: Results from CANWARD 2007 by Patricia J Baudry, Melissa McCracken, Philippe Lagacé-Wiens, Michael R Mulvey, George G Zhanel, Daryl J Hoban

“…CONCLUSION: CTX-M has become the most common enzyme among both ESBL-producing E coli and K pneumoniae. …”
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Levo-Tetrahydropalmatine Attenuates Bone Cancer Pain by Inhibiting Microglial Cells Activation by Mao-yin Zhang, Yue-peng Liu, Lian-yi Zhang, Dong-mei Yue, Dun-yi Qi, Gong-jian Liu, Su Liu

“…The concentrations of TNF-α, IL-1β, and IL-18 in spinal cord were measured by enzyme-linked immunosorbent assay. Western blot was used to test the activation of astrocytes and microglial cells in spinal cord after TCI treatment. …”
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Cell–cell heterogeneity in phosphoenolpyruvate carboxylase biases early cell fate priming in Dictyostelium discoideum by Kenichi Abe, Hidenori Hashimura, Haruka Hiraoka, Shoko Fujishiro, Narufumi Kameya, Kazuteru Taoka, Satoshi Kuwana, Masashi Fukuzawa, Satoshi Sawai, Satoshi Sawai, Satoshi Sawai

“…Our results suggest the importance of cell–cell heterogeneity in the levels of metabolic enzymes during early cell fate priming.…”
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Positive feedback loop involving AMPK and CLYBL acetylation links metabolic rewiring and inflammatory responses by Wenke Wang, Boquan Wu, Mingjun Hao, Sichong Chen, Ruiting Cong, Wenjie Wu, Pengbo Wang, Qiaoyi Zhang, Pengyu Jia, Yuequn Song, Bo Liu, Siyao Qu, Jian-Fei Pei, Da Li, Naijin Zhang

“…Mechanistically, we found a crucial AMPK-CLYBL acetylation positive feedback loop, triggered by toll-like receptors (TLRs), involving AMPK hypophosphorylation and CLYBL hyperacetylation. The deacetylase enzyme SIRT2 acted as the bridge between AMPK phosphorylation and CLYBL acetylation, thereby regulating macrophage polarization and the release of pro-inflammatory cytokines. …”
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Investigating the Effect of Testosterone Supplement on Heart and Muscle Damage in Rats Applied with Swimming Exercise by Ömer Kulaksız, Sefa Lök

“…When between-groups values of troponin I, ck-MB, and LDH, which are indicators of heart damage, and enzyme CK, which is an indicator of muscle damage,for control, exercise, testosterone, and testosterone+exercise groups were compared, a statistically significant difference was determined between control, exercise, testosterone, and testosterone+exercise groups (p lt;0.05). …”
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