Flexibility in PAM recognition expands DNA targeting in xCas9 by Kazi A Hossain, Lukasz Nierzwicki, Modesto Orozco, Jacek Czub, Giulia Palermo

“…xCas9 is an evolved variant of the CRISPR-Cas9 genome editing system, engineered to improve specificity and reduce undesired off-target effects. …”
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Benchmarking the identification of a single degraded protein to explore optimal search strategies for ancient proteins by Rodriguez Palomo, Ismael, Nair, Bharath, Chiang, Yun, Dekker, Joannes, Dartigues, Benjamin, Mackie, Meaghan, Evans, Miranda, Macleod, Ruairidh, Olsen, Jesper V., Collins, Matthew J.

“…We systematically investigated the performance of a wide range of sequencing tools and search engines in a controlled system: the experimental degradation of the single purified bovine β-lactoglobulin (BLG), heated at 95°C and pH 7 for 0, 4 and 128 days. …”
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The Glocalization of Sport: A Research Field for Social Innovation by Zijing Li, Arnaud Waquet, Philippe Campillo

“…The glocalization, considered as a theorical framework to rethink local social innovation which answered to the limit of the globalization, is examined in this article through an in-depth bibliographic analysis conducted using the Lillocat metasearch engine (covering 354 articles since 1992 across 11 thematic areas, 29 of which are related to sports). …”
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Induced hepatocyte-like cells derived from adipose-derived stem cells alleviates liver injury in mice infected with Echinococcus Multilocularis by Hui Liu, Xiaojuan Bi, Ning Yang, Xue Zhang, Bingbing Fang, Nuerbaiti Kusuman, Wenmei Ma, Jing Li, Jin Chu, Li Sun, Liang Li, Guodong Lü, Renyong Lin

“…Abstract Accumulating evidence has shown that adipose-derived stem cells (ADSCs) have the potential to differentiate into hepatic lineages, which are ideal engraftments for tissue-engineered repair. In this study, we investigated the potential of transplanted induced hepatocyte-like cells (iHEPs) in treating hepatic alveolar echinococcosis and describe an efficient three-step protocol for the generation of iHEPs in vitro from ADSCs. …”
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Non-Stoichiometric Ba_xMn_0.7Cu_0.3O₃ Perovskites as Catalysts for CO Oxidation: Optimizing the Ba Content by Álvaro Díaz-Verde, Emerson Luiz dos Santos Veiga, Héctor Beltrán-Mir, María José Illán-Gómez, Eloísa Cordoncillo-Cordoncillo

“…The Ba_0.8Mn_0.7Cu_0.3O₃ composition (B0.8MC) showed the best catalytic performance (comparable to that of the 1% Pt/Al₂O₃ reference sample) during tests conducted under conditions similar to those found in the exhaust gases of current gasoline engines. The characterization data suggest the simultaneous presence of a high Mn(IV)/Mn(III) surface ratio, oxygen vacancies, and reduced copper species, these two latter being key properties for ensuring a high CO conversion percentage as both are active sites for CO oxidation. …”
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The role of epigenetics in plant pathogens interactions under the changing environments: A systematic review by Wenhao Wu, Guoqiang Fan

“…Advances in high-throughput sequencing and CRISPR-based tools are shedding light on these complex interactions, offering opportunities to engineer crops with enhanced disease resistance. Furthermore, the review emphasizes the need to explore the interplay between these epigenetic mechanisms under multifaceted environmental stresses. …”
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Lower Extremity Peripheral Arterial Disease Is an Independent Predictor of Coronary Heart Disease and Stroke Risks in Patients with Type 2 Diabetes Mellitus in China by Xiao-Hong Pang, Jue Han, Wan-Lan Ye, Xue Sun, Yue Ding, Wen-Juan Huang, Yi-Ming Zhao, Han-Yu Lou, Li-Zhen Shan, Ying-Xiu Kang, Xiao-Xiao Song, Song-Zhao Zhang, Wei Gu, Peng-Fei Shan

“…We aimed to determine the relationship between lower extremity peripheral arterial disease (PAD), 10-year coronary heart disease (CHD), and stroke risks in patients with type 2 diabetes (T2DM) using the UKPDS risk engine. We enrolled 1178 hospitalized T2DM patients. …”
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Structural basis of FpGalNase and its combination with FpGalNAcDeAc for efficient A-to-O blood group conversion by Meiling Zhou, Kaishan Luo, Chao Su, Yan Sun, Zuyan Huang, Shuo Ma, Xun Gao, Jiwei Wang, Chen Zhang, Pengcheng Han, Guoqiu Wu

“…We optimized treatment conditions, achieving over 99% conversion in just five minutes using phosphate buffer saline and a 16 nM enzyme concentration. Additionally, we engineered two fusion proteins, FpGalNAcDeAc-FpGalNase and FpGalNase-FpGalNAcDeAc, which showed a 28-fold increase in catalytic efficiency compared to the enzyme mixture. …”
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Treatment Approaches to Speech Sound Disorders: A Scoping Review by Hafsa Noreen, Nazia Mumtaz, Ghulam Saqulain

“…Methodology: To find the different therapeutic approaches, a literature search for related articles/ publications was conducted using the Keywords “speech sound disorders, phonological disorders, articulation disorder and a combination of words using search engines like Google & and databases like PubMed and other websites.  …”
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Query transformations and their role in Web searching by the members of the general public by Martin Whittle, Barry Eaglestone, Nigel Ford, Valerie J. Gillet, Andrew Madden

“…</b> In this work, we have used transaction logs from the Excite search engine to develop methods for analysing query transformations that should aid the analysis of our ongoing experimental work. …”
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hsa_circ_0001599 promotes odontogenic differentiation of human dental pulp stem cells by increasing ITGA2 expression and stability by Yeqing Yang, Chong Jiang, Junkai Zeng, Xiaolan Guo, Ming Chen, Buling Wu

“…This research revealed the important role of hsa_circ_0001599 in odontogenic differentiation. Thus, hDPSCs engineered with hsa_circ_0001599 have the potential to be effective therapeutic targets for dental pulp repair and regeneration.…”
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A review on bioremediation by microbial immobilization-an effective alternative for wastewater treatment by Frank Abimbola Ogundolie, Olorunfemi Oyewole Babalola, Charles Oluwaseun Adetunji, Christiana Eleojo Aruwa, Jacqueline Njikam Manjia, Taoheed Kolawole Muftaudeen

“…Furthermore, our work stands out by highlighting emerging state-of-the-art tools that are bioinspired [enzymes, reactive permeable barriers linked to electrokinetic, magnetic cross-linked enzyme aggregates (CLEAs), bio-coated films, microbiocenosis], as well as the use of nanosized biochar and engineered cells or their bioproducts targeted at enhancing the removal efficiency of metals, carbonates, organic matter, and other toxicants and pollutants. …”
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The Role of Gut Microbiota in Tumor Immunotherapy by Miao Wu, Jiawei Bai, Chengtai Ma, Jie Wei, Xianjin Du

“…With the development of gene editing technology and nanotechnology, the design and development of engineered bacteria that contribute to immunotherapy has become a new research hotspot. …”
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Physics-Based Self-Supervised Grasp Pose Detection by Jon Ander Ruiz, Ander Iriondo, Elena Lazkano, Ander Ansuategi, Iñaki Maurtua

“…Given a gripper-object pair, it samples grasping pose candidates using a modified version of GPD (implementing inner-grasps, CAD support…) and quantifies their quality using the MuJoCo physics engine and a grasp quality metric that takes into account the pose of the object over time. …”
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The Discriminative Lexicon: A Unified Computational Model for the Lexicon and Lexical Processing in Comprehension and Production Grounded Not in (De)Composition but in Linear Discr... by R. Harald Baayen, Yu-Ying Chuang, Elnaz Shafaei-Bajestan, James P. Blevins

“…The discriminative lexicon also incorporates the insight from machine learning that end-to-end modeling is much more effective than working with a cascade of models targeting individual subtasks. The computational engine at the heart of the discriminative lexicon is linear discriminative learning: simple linear networks are used for mapping form onto meaning and meaning onto form, without requiring the hierarchies of post-Bloomfieldian ‘hidden’ constructs such as phonemes, morphemes, and stems. …”
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ON IMPROVING THE ENERGY SUPPLY OF BATCH DYEING APPARATUSES IN THE LIGHT INDUSTRY ENTERPRISES by V. N. Romaniuk, D. B. Muslina

“…Another critical factor of the presented analysis and ways for thermo-technological energy supply improvement is that the proposed changes can realize on basis of the equipment that established a good reputation during continuous service in different productions and manufactured in the republic in working partnership with foreign design engineers.…”
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Determination of Selected Polycyclic Aromatic Compounds in Particulate Matter Samples with Low Mass Loading: An Approach to Test Method Accuracy by Susana García-Alonso, Rosa María Pérez-Pastor, Víctor Archilla-Prat, David Sanz-Rivera, Enrique Rojas-García, Gaizka Aragón-Urrengoetxea, Jesús Javier Rodríguez-Maroto

“…The effectiveness of the proposed method was evaluated in PM aerosol samples collected with very low mass loadings (<0.2 mg) during characterization studies from turbofan engine exhausts.…”
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Combined MSC and GLP-1 Therapy Modulates Collagen Remodeling and Apoptosis following Myocardial Infarction by Elizabeth J. Wright, Nigel W. Hodson, Michael J. Sherratt, Moustapha Kassem, Andrew L. Lewis, Christine Wallrapp, Nadim Malik, Cathy M. Holt

“…We examined the effects of MSCs, either native, or engineered to secrete a GLP-1 fusion protein (MSCs ± GLP-1), on human cardiomyocyte apoptosis in vitro. …”
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Rapid growing mass of the mandible due to an oral metastasis of thymoma: Case report of an extremely rare localization and review of published cases by Francesco Scilla, Cosimo Rupe, Gioele Gioco, Luca Raffaelli, Filippo Lococo, Ciro Mazzarella, Guido Rindi, Romeo Patini, Carlo Lajolo

“…Thus, a final diagnosis of intraoral metastasis of thymoma was achieve.A review of the literature of oral metastases due to thymic tumor was also conducted through a systematic search in accordance with PRISMA guidelines, via PubMed, Scopus and CENTRAL engines. Out of 328 articles retrieved, only two articles reported oral metastasis of thymic tumors; and two reports of ectopic thymic carcinoma of the parotid gland were found. …”
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The ‘Agency of Mapping’ in South Asia: Galle-Matara (Sri Lanka), Mumbai (India) and Khulna (Bangladesh) by Kelly Shannon

“…The territories/cities investigated are the southwest (Galle-Matara) coast of Sri Lanka, Mumbai, the economic engine of India, and Khulna, the third largest city in Bangladesh.…”
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