Increasing Donor Endothelial Cell Pool by Culturing Cells from Discarded Pieces of Human Donor Corneas for Regenerative Treatments by Mohit Parekh, Vito Romano, Alessandro Ruzza, Stephen B. Kaye, Diego Ponzin, Sajjad Ahmad, Stefano Ferrari

“…To investigate if the peripheral corneal endothelium that is usually discarded after a corneal transplant could be used for endothelial cell culture. Methods. Donor corneas (n = 19) with a mean age of 72 years, male : female ratio of 15 : 4, and death-to-preservation time of 10 hours were assessed for endothelial cell density (ECD) and number of dead cells before isolation. …”
Get full text

Extracellular vesicle surface engineering with integrins (ITGAL & ITGB2) to specifically target ICAM-1-expressing endothelial cells by Markus Bergqvist, Kyong-Su Park, Nasibeh Karimi, Lijuan Yu, Cecilia Lässer, Jan Lötvall

“…Further, activated human endothelial cells treated with LFA-1 EVMyd88 had increased uptake of these EVs, resulting in dose-dependent inhibition of induced release of IL-8, presumably by targeting Myd88. …”
Get full text

Verisyse versus Veriflex Phakic Intraocular Lenses: Refractive Outcomes and Endothelial Cell Density 5 Years after Surgery by Dilek Yaşa, Alper Ağca

“…To compare refractive stability, central endothelial cell density (ECD), and complications between Verisyse (Abbott Medical Optics, Netherlands) and Veriflex (Abbott Medical Optics, Netherlands) phakic intraocular lenses (pIOL) over five years. …”
Get full text

Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury by Jipeng Ouyang, Rong Li, Haiqin Shi, Jianping Zhong

“…In the present study, human umbilical vein endothelial cells (HUVECs) were pretreated with various concentrations of curcumin (0 μM, 10 μM, 20 μM, 30 μM, 40 μM, and 50 μM) for 12 h, thereby exposed to H2O2 (100 μM) for another 12 h. …”
Get full text

Paeoniflorin alleviates high glucose-induced endothelial cell apoptosis in diabetes mellitus by inhibiting HRAS-activated RAS pathway by Wenting Yu, Hongchun Jiang

“…Human umbilical vein endothelial cells (HUVECs) were separately treated with HG, Pae (5, 10, 20 μM) and/or HRAS overexpression plasmids (oe-HRAS). …”
Get full text

17β-estradiol upregulates striatin protein levels via Akt pathway in human umbilical vein endothelial cells. by Shuhui Zheng, Peng Sun, Haimei Liu, Runmei Li, Lingli Long, Yuxia Xu, Suiqing Chen, Jinwen Xu

“…The objective of the present study was to investigate the effects of E2 on the regulation of striatin expression in human umbilical vein endothelial cells (HUVECs). We demonstrated that E2 increased striatin protein expression in a dose- and time-dependent manner in HUVECs. …”
Get full text

Effects of Combinations of Ophthalmic Viscosurgical Devices and Suction Flow Rates on the Corneal Endothelial Cell Damage Incurred during Phacoemulsification by Tomoyuki Kunishige, Hiroshi Takahashi

“…The reduction rate of the endothelial cell density at the center of the cornea was significantly higher in the V group (4.1% ± 6.7%) than the S group (0.3% ± 4.5%; p=0.03) at 1 week postoperatively. …”
Get full text

Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq by Shintaro Funasaki, Yuri Miyamura, Shunsuke Kamei, Akhinur Rahman, Masaya Yamazaki, Shingo Usuki, Keiichiro Yasunaga, Yorifumi Satou, Hiroto Ohguchi, Takashi Minami

“…Here, we present a protocol for transcriptomic and epigenomic analyses of tip-like cells in cultured endothelial cells. We describe steps for stimulating human umbilical vein endothelial cells (HUVECs) with vascular endothelial cell growth factor (VEGF) to generate tip-like cells. …”
Get full text

Modulation of Inflammatory Reactions by Low-Dose Ionizing Radiation: Cytokine Release of Murine Endothelial Cells Is Dependent on Culture Conditions by Sabine Schröder, Stephan Kriesen, Daniel Paape, Guido Hildebrandt, Katrin Manda

“…The objective of this study was to gain a better insight into the modulation of inflammatory reactions after LD-RT on the basis of endothelial cells (EC) as major participants and regulators of inflammation. …”
Get full text

Sickle Cell Disease Activates Peripheral Blood Mononuclear Cells to Induce Cathepsins K and V Activity in Endothelial Cells by Philip M. Keegan, Sindhuja Surapaneni, Manu O. Platt

“…Cathepsins are powerful elastases and collagenases secreted by endothelial cells and monocyte-derived macrophages in atherosclerosis, but their involvement in sickle cell disease has not been studied. …”
Get full text

Overexpressed Neuropilin-1 in Endothelial Cells Promotes Endothelial Permeability through Interaction with ANGPTL4 and VEGF in Kawasaki Disease by Junhua Huang, Shuwan Zhang

“…Here, we found that sera from patients with KD can induce endothelial cell (EC) hyperpermeability compared to sera from healthy controls. …”
Get full text

Mitochondrial apoptosis in response to cardiac ischemia-reperfusion injury by Kaixin Wang, Qing Zhu, Wen Liu, Linyuan Wang, Xinxin Li, Cuiting Zhao, Nan Wu, Chunyan Ma

Subjects: Get full text

Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor by Mohammad Amir Mishan, Sahar Balagholi, Tahereh Chamani, Sepehr Feizi, Zahra-Soheila Soheili, Mozhgan Rezaei Kanavi

Subjects: Get full text

IFN-γ reprograms cardiac microvascular endothelial cells to mediate doxorubicin transport and influences the sensitivity of mice to doxorubicin-induced cardiotoxicity by Haoyu Ji, Wenya Ma, Xu Liu, Hongyang Chen, Yining Liu, Zhongyu Ren, Daohong Yin, Ao Cai, Zizhen Zhang, Xin Wang, Wei Huang, Leping Shi, Yanan Tian, Yang Yu, Xiuxiu Wang, Yang Li, Yu Liu, Benzhi Cai

“…Single-cell RNA sequencing revealed differences of gene expression in cardiac endothelial cells between DIC-insensitive and DIC-sensitive groups. …”
Get full text

Corrigendum to “MicroRNA-30-5p Suppresses Inflammatory Factor-Induced Endothelial Cell Injury by Targeting TCF21” by Zhenyu Zhou, Yu Chen, Dongying Zhang, Shiyong Wu, Tao Liu, Guoqiang Cai, Shu Qin

Get full text

Exosomes Derived from CXCR4-Overexpressing BMSC Promoted Activation of Microvascular Endothelial Cells in Cerebral Ischemia/Reperfusion Injury by Xutong Li, Ye Zhang, Yong Wang, Dan Zhao, Chengcheng Sun, Shaoting Zhou, Dongsheng Xu, Jing Zhao

“…In ischemia/reperfusion injury, ExoCXCR4 promoted the proliferation and tube formation of microvascular endothelial cells and play an antiapoptotic role via the Wnt-3a/β-catenin pathway.…”
Get full text

Human Aortic Endothelial Cell Labeling with Positive Contrast Gadolinium Oxide Nanoparticles for Cellular Magnetic Resonance Imaging at 7 Tesla by Yasir Loai, Nurus Sakib, Rafal Janik, Warren D. Foltz, HL Cheng

“…Relaxivity measurements yielded r 1 = 4.7 s −1 mM −1 and r 2 / r 1 = 6.2. Human aortic endothelial cells were labeled with Gd 2 O 3 at various concentrations and underwent MRI from 1 to 7 days postlabeling. …”
Get full text

Epac1 Blocks NLRP3 Inflammasome to Reduce IL-1β in Retinal Endothelial Cells and Mouse Retinal Vasculature by Youde Jiang, Li Liu, Elizabeth Curtiss, Jena J. Steinle

“…We previously reported that a novel β-adrenergic receptor agonist, Compound 49b, reduced Toll-like receptor 4 (TLR4) signaling in retinal endothelial cells (REC) grown in high glucose. Others reported that TLR4 activates high-mobility group box 1 (HMGB1), which has been associated with the NOD-like receptor 3 (NLRP3) inflammasome. …”
Get full text

Trimethylamine-N-Oxide Promotes High-Glucose-Induced Dysfunction and NLRP3 Inflammasome Activation in Retinal Microvascular Endothelial Cells by Lidan Xue, Lili Huang, Yajing Tian, Xin Cao, Yu Song

“…Human retinal microvascular endothelial cells (HRMECs) were treated for 72 h as follows: NG (normal glucose, D-glucose 5.5 mM), NG + TMAO (5 μM), HG (high glucose, D-glucose 30 mM), and HG + TMAO (5 μM). …”
Get full text

Retracted: Loading Gentamicin and Zn2+ on TiO2 Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities by Stem Cells International

Get full text