A stratified two-stage tumor molecular profiling algorithm to identify clinically actionable molecular alterations in pancreatic cancer☆ by S. Hussung, D. Akhoundova, C. Pistoni, D. Lenggenhager, A. Töpfer, C. Pauli, B. Pestalozzi, C. Britschgi, M. Zoche, M. Rechsteiner, H. Moch, A. Weber, R. Fritsch

“…Stage 1 comprised immunohistochemistry for mismatch repair deficiency and targeted sequencing employing a 33-gene next-generation sequencing panel covering common PC drivers and DNA damage response genes. …”
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Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision by Antonis Perdiou, Ramona Dumitrescu, Daniela Jumanca, Octavia Balean, Ruxandra Sava-Rosianu, Serban Talpos, Dacian Virgil Lalescu, Atena Galuscan

“…Autofluorescence imaging was performed using the OralID device, which employs a 405 nm excitation laser to detect abnormal tissue. …”
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Considerations for determining warm-water coral reef tipping points by P. Pearce-Kelly, A. H. Altieri, J. F. Bruno, C. E. Cornwall, M. McField, A. I. Muñiz-Castillo, J. Rocha, R. O. Setter, C. Sheppard, R. M. Roman-Cuesta, C. Yesson

“…Uncertainties around tipping points for such crucially important ecosystems underline the imperative of robust assessment and, in the case of knowledge gaps, employing a precautionary principle favouring lower-range tipping point values.…”
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Recognition of HER2 expression in hepatocellular carcinoma and its significance in postoperative tumor recurrence by Ji‐Hua Shi, Wen‐Zhi Guo, Yang Jin, Hua-Peng Zhang, Chun Pang, Jie Li, Pål‐Dag Line, Shui‐Jun Zhang

“…Western Blotting and immunohistochemistry were employed to test expression of HER2, E‐cadherin, and Vimentin. …”
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A combined wavelet analysis-quantile mapping (WA-QM) method for bias correction: capturing the intra-annual temporal patterns in climate model precipitation simulations and project... by Xia Wu, Zhu Liu, Qingyun Duan

“…Furthermore, WA could be an effective tool to overcome QM’s limitation in capturing intra-annual temporal patterns. The WA approach employed discrete wavelet transformation to decompose GCM data into various frequency bands and then adjusted their standard deviations and signs to ensure that their relative relationships were consistent with those in observations. …”
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A semantic strength and neural correlates in developmental dyslexia by Sladjana Lukic, Sladjana Lukic, Sladjana Lukic, Fei Jiang, Maria Luisa Mandelli, Maria Luisa Mandelli, Ting Qi, Sarah M. Inkelis, Sarah M. Inkelis, Emily Rosenthal, Emily Rosenthal, Zachary Miller, Zachary Miller, Emma Wellman, Emma Wellman, Silvia A. Bunge, Silvia A. Bunge, Silvia A. Bunge, Maria Luisa Gorno-Tempini, Maria Luisa Gorno-Tempini, Christa Watson Pereira, Christa Watson Pereira

“…First, we adjusted performance on semantic fluency by letter fluency and created a residual score that was used to separate participants into high (residual >0) or average (residual <0) semantic performance groups. We then employed a psycholinguistic clustering and switching approach to the semantic fluency task and performed dynamic task-free rs-fMRI connectivity analysis to reveal group differences in brain dynamics.ResultsHigh and average semantic fluency groups were well-matched on demographics and letter fluency but differed on their psycholinguistic patterns on the semantic fluency task. …”
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Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece by Anestis Tsitsos, Alexandros Damianos, Konstantinos Kiskinis, Vasilios Tsiouris, Ilias Tirodimos, Nikolaos Soultos, Anna Papa, Vangelis Economou

“…Strains were selectively isolated and identified via MALDI-TOF MS, which was also employed to identify possible relatedness. <i>E. coli</i> isolates were further classified into phylogenetic groups. …”
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Influence of Irradiance, Flow Rate, Reactor Geometry, and Photopromoter Concentration in Mineralization Kinetics of Methane in Air and in Aqueous Solutions by Photocatalytic Membra... by Ignazio Renato Bellobono, Mauro Rossi, Andrea Testino, Franca Morazzoni, Riccardo Bianchi, Giulia de Martini, Paola Maria Tozzi, Rodica Stanescu, Cristina Costache, Liliana Bobirica, Mauro Luigi Bonardi, Flavia Groppi

“…A kinetic model was employed, from which, by a set of differential equations, four final optimised parameters, k1 and K1, k2 and K2, were calculated, which is able to fit the whole kinetic profile adequately. …”
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Analysis of Soil Microbial Community Structure and Function in <i>Morchella esculenta</i> Habitats in Jilin Province by Qi Yan, Peng Wang, Zhushan Liu, Ya Yu, Xiao Tan, Xiao Huang, Jiawei Wen, Weidong Zhang

“…Metagenome sequencing technology was employed to analyze the structure and function of the rhizosphere microbial communities. …”
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Eco-friendly synthesis of silver nanoparticles: Enhancing optimization reaction, characterization, and antimicrobial properties with Lantana camara from geothermal area by Pati Kemala, Rinaldi Idroes, Khairan Khairan, Muliadi Ramli, Binawati Ginting, Zuchra Helwani, Rifki Aulia, Ghazi Mauer Idroes, Muhammad Yusuf, Rustam Efendi

“…Reaction optimization was performed using response surface methodology (RSM), employing a central composite design (CCD) approach. …”
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Stem Cells from Human Exfoliated Deciduous Teeth Ameliorate Diabetic Nephropathy In Vivo and In Vitro by Inhibiting Advanced Glycation End Product-Activated Epithelial-Mesenchymal... by Nanquan Rao, Xiaotong Wang, Jing Xie, Jingzhi Li, Yue Zhai, Xiaoxia Li, Tengjiaozi Fang, Yuanyuan Wang, Yuming Zhao, Lihong Ge

“…At 8 weeks after administration, real-time PCR, immunohistochemistry (IHC), and electron microscopy were employed to examine pathological changes in glomerular and tubulointerstitial tissue. …”
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Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. …”
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Assessment approach for conservation effectiveness and gaps for endangered species based on habitat suitability: A case study of alpine musk deer in western China by Feng Jiang, Pengfei Song, Jingjie Zhang, Dongwu Wang, Renhong Li, Chengbo Liang, Tongzuo Zhang

“…This study integrated data from 274 alpine musk deer occurrence points, obtained through infrared camera monitoring, field surveys, and literature reviews, with environmental variables, employing the MaxEnt model to analyze the current spatial distribution of suitable habitats and priority conservation areas in Western China. …”
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Causal associations between osteoporosis and HBV infection across Asian and European populations: evidence from Mendelian randomization and colocalization analysis by Zhengnan Li, Jiarui Cao, Ke Li, Yixin Wu, Zhanpeng Luo, Rui Cao, Zhiheng Cheng, Zhendong Tian, Yiyang Han, Yuping Lai, Bangqi Wang, Shen Chen

“…Various MR methods, including inverse variance weighted (IVW), MR Egger, weighted median, simple median and simple mode were employed to estimate the association between HBV infection and osteoporosis. …”
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Manganese-based immunotherapy synergized with novel supramolecular hydrogel: Advancing localized immune activation strategies in squamous cell carcinoma by Tianqing Wang, Xiaopei Hu, Xin Tian, Ying Wang, Shiyu Zhang, Silu Sun, Ning Ji, Xiaobo Luo, Qianming Chen, Xin Zeng, Hang Zhao, Jing Li

“…In this study, we introduce a novel approach employing manganese-based immunotherapy (Mn-IT) for the effective treatment of HNSCC. …”
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Predicting breast cancer prognosis using PR and PIK3CA biomarkers: a comparative analysis of diagnostic groups by Yuting Feng, Qingzhen Song, Lei Yan, Ruoqi Li, Mengqin Yang, Peng Bu, Jing Lian

“…To evaluate the variability of clinicopathologic features among these groups, t tests and ANOVA were employed. The influence of these variables on survival was analyzed via Cox regression. …”
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A systematic review and mapping exercise to assess the content validity of patient-reported outcome measures for adults having reconstructive surgery of the lower limb by Jennie Lister, Sophie James, Hemant K. Sharma, Catherine Hewitt, Helen Fulbright, Heather Leggett, Catriona McDaid

“…Many patient-reported outcome measures (PROMs) are employed to assess these impacts; however, there are concerns that they do not adequately capture all outcomes important to patients, and may lack content validity in this context. …”
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Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network by Qi Xiao, Jingjing Shi, Lixian Wang, Guoping Zhao, Yanfei Zhang

“…While enhanced production of isopentanol has been achieved in a variety of chassis, the fermentation yield has not yet reached levels suitable for industrial-scale production. In this study, we employed a continuous perturbation tool to construct a genome-scale perturbation library, combined with an isopentanol biosensor to screen for high-yielding mutants. …”
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Gene networks for use in metabolomic data analysis of blood plasma from patients with postoperative delirium by V. A. Ivanisenko, N. V. Basov, A. A. Makarova, A. S. Venzel, A. D. Rogachev, P. S. Demenkov, T.  V. Ivanisenko, M. A. Kleshchev, E. V. Gaisler, G. B. Moroz, V. V. Plesko, Y. S. Sotnikova, Y. V. Patrushev, V. V. Lomivorotov, N. A. Kolchanov, A. G. Pokrovsky

“…The analysis revealed four significantly different sphingomyelin contents in patients with POD compared to those who did not develop POD (control group). Employing gene network reconstruction, we perceived a set of 82 regulatory enzymes affiliated with the genetic coordination of the sphingolipid metabolism pathway. …”
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Biorefinery potential of coffee silverskin: Composition and applications by Elena M. Anchugova, Elena V. Udoratina, Elena G. Kazakova, Anastasiia O. Nosova, Mayya V. Uspenskaya, Tatiana N. Shchemelinina

“…This paper aims to analyze the potential of CS for biorefinery and explore its value enhancement through biotechnology. Method: The study employed various analytical techniques, including elemental analysis, chromatography methods, and Fourier transform methods, to characterize the CS biomass. …”
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