GsMTx-4 venom toxin antagonizes biophysical modulation of metastatic traits in human osteosarcoma cells by Arianna Buglione, Giulia Alloisio, Chiara Ciaccio, David Becerril Rodriguez, Simone Dogali, Marco Luce, Stefano Marini, Antonio Cricenti, Magda Gioia

“…To elucidate the molecular mechanisms that translate biomechanical alterations into biochemical responses, we employed Western blotting, real-time quantitative RT-PCR, reactive oxygen species ROS assay, and the mechanosensitive channel blocker Grammostola MechanoToxin4 (GsMTx-4). …”
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Impact of landscape patterns on river water quality: Spatial-scale effects across an agricultural-urban interface by Kun Mei, Haonan Shi, Yupeng Wu, Randy A. Dahlgren, Xiaoliang Ji, Minmin Yang, Yueru Guan

“…Regression models were used to investigate the quantitative impact of landscape patterns on water quality, redundancy analysis was employed to assess the overall explanatory power, and non-parametric point-of-change analysis was applied to evaluate variations in water quality along landscape gradients and identify critical landscape threshold ranges. …”
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Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis by Wenli Xu, Lu Li, Hong Kang, Meixian Wang, Yanqun Liu, Guicun Wang, Ping Yu, Juan Liang, Zhen Liu

“…The 16 S rDNA gene sequencing and metabolomics methods were employed to assess 51 amniotic fluid samples. The amniotic fluid microbiome from the CHDs group exhibited significantly higher Shannon and Simpson indices compared to the control group. …”
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Practice and factors affecting informed consent among healthcare workers for major surgical procedures at Gurage zone hospitals, South Ethiopia, 2022: a facility-based cross-sectio... by Haimanot Abebe, Bitew Tefera Zewudie, Yibeltal Mesfin, Shegaw Geze Tenaw, Daniel Tsega, Bogale Chekole, Zebene Mekonnen, Shegaw Tesfa Mengist, Agere Aynalem, Tadele Lankrew, Yihenew Sewale, Muche Argaw, Tadesse Sahle

“…Objective This study aimed to assess the informed consent practice process and associated factors among healthcare workers for major surgical procedures at Gurage zone hospitals, in 2022.Methods and materials Institution-based cross-sectional study was employed.Settings This study was conducted in Gurage zone hospitals.Participants All healthcare workers who have been working in Gurage zone hospitals of surgical, operation rooms and obstetrics/gynaecology wards had exposure to patients who had undergone surgery during the study periodOutcome The primary outcome of the study was the practice of informed consent practice among healthcare workers in Gurage zone hospitals.Result A total of 448 study participants were involved in this study giving a response rate of 98%. …”
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Polymeric Multivalent Fc Binding Peptides‐Fabricated Clinical Compounding Bispecific Antibody Potentiates Dual Immunotherapy Targeting PD1 and CTLA‐4 by Zongyu Liu, Hongyu Chu, Weidong Zhao, Chenguang Yang, Tongjun Liu, Na Shen, Zhaohui Tang

“…In this study, polymeric multivalent Fc‐binding peptides (PLG‐Fc‐III‐4C) are employed to fabricate a bispecific antibody (PD1/CTLA‐4 BsAb) to potentiate dual immunotherapy targeting PD1 and CTLA‐4. …”
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High nature value farming systems in Europe: A dataset encompassing the environmental impact assessment of farms and extensive ruminant food productsFigshare by M. Torres-Miralles, P. Jeanneret, M. Lamminen, F. Joly, B. Dumont, H. Tuomisto, I. Herzon

“…To address this gap, we established an international collaborative network across Europe, involving professionals directly engaged with farmers, farmer associations, and researchers to collect data on HNV farms employing a developed questionnaire examining inputs and outputs, farm structures, and herd characteristics. …”
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Strain rebound and inhomogeneity in glass-to-metal seals: Radial vs axial strain evolution by Keqian Gong, Zheng Liu, Zifeng Song, Chao Zhou, Zhangjing Shi, Siyue Nie, Weisong Zhou, He Yan, Zhichun Fan, Yong Zhang

“…Despite extensive studies on residual stress/strain in sealing glass, critical gaps remain in decoupling directional strains and understanding their in situ evolution during the cooling process, especially with respect to their microstructural origins. This study employs advanced fiber Bragg grating (FBG) in situ monitoring to decouple and independently analyze radial and axial strain evolution, providing novel insights into the mechanical anisotropy of sealing glass. …”
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Association between hemoglobin glycation index and poor outcome after endovascular thrombectomy in acute ischemic stroke by Yan Yang, Mei Liu, Shungui Huang, Chen Zhu, Guangzong Li, Bin Wang, Xiaojing Luo, Lingwen Zhang, Weizheng Song

“…We used multivariable logistic regression models to investigate the association between HGI and poor outcome. We employed the restricted cubic spline curve to visualize the association between HGI and the risk of poor outcome after EVT.ResultsAmong the 403 enrolled patients (median age, 72 years; 63.8% male), a total of 198 (49.1%) patients had poor outcome at 90 days. …”
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The Role of Motivation to Promote Personal and Social Responsibility Through PE and Sport: A Comparative Exploratory Investigation of Prospective PE Teachers and Sports Leaders in... by Istvan Soos, Ian Whyte, Diana Szekeres, Karoly Ozsvath, Attila Szabo, Jonathan Ling, Jose Francisco Jiménez-Parra, Alfonso Valero-Valenzuela

“…Discussion: We suggest that a hybridised model incorporating Teaching Personal and Social Responsibility and the Sports Education Model is employed with sport and physical education students to optimise potential for transfer of values and behaviours.…”
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Use of Digital Health Interventions for Cancer Prevention Among People Living With Disabilities in the United States: A Scoping Review by Chinenye Lynette Ejezie, Lea Sacca, Sylvia Ayieko, Sara Burgoa, Yasmine Zerrouki, Diana Lobaina, Goodness Okwaraji, Christine Markham

“…Reports published in English of studies that employed digital health strategies for cancer prevention, were conducted among people with disabilities regardless of age, and were conducted in the United States were included. …”
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Precision Nutrient and Soil Tillage Management for Sustainable Winter Barley Production (<i>Hordeum vulgare</i> L.) and Tillage Impact on Soil CO₂ Emission by Amare Assefa Bogale, Zoltan Kende, Akos Tarnawa, Peter Miko, Marta Birkás, Gergő Péter Kovács, Attila Percze

“…The study aimed to evaluate the effects of soil tillage and foliar nutrient supplementation on winter barley yield, associated characteristics, and soil CO₂ emissions. Employing a split-plot design with three replications, the experiment included four nutrient treatments (control, bio-cereal, bio-algae, and MgSMnZn blend) and two soil tillage type (i.e., plowing and cultivator). …”
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Milk Composition Is Predictive of Low Milk Supply Using Machine Learning Approaches by Xuehua Jin, Ching Tat Lai, Sharon L. Perrella, Xiaojie Zhou, Ghulam Mubashar Hassan, Jacki L. McEachran, Zoya Gridneva, Nicolas L. Taylor, Mary E. Wlodek, Donna T. Geddes

“…Machine learning algorithms were employed to develop prediction models integrating milk composition and maternal and infant characteristics. …”
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Identification of compounds against atherosclerosis induced by ox-LDL based on cell extraction/UPLC–MS/MS from mulberry twigs and their mechanistic analysis by Zhihui Jin, Wei Xiang, Xiaowen Shi, Fenglin Jiang, Yanan Jia, Yuansong Zhang, Lingshu Zeng, Xianzhi Huang, Li Xu

“…Methods Biospecific cell extraction and ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) were employed to screen and identify these compounds. Protective effects were assessed by measuring cell viability, malondialdehyde (MDA), and superoxide dismutase (SOD) levels, along with detecting intracellular reactive oxygen species (ROS) using 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) and dihydroethidine (DHE) probes. …”
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Effects of concave thoracoplasty on chest circumference and ventilatory function in adolescence with idiopathic scoliosis by Khaled Takey Ahmed, Abla Mohamed Hamed, Youssry El Hawary, Nesreen G. El-Nahas, Akram M. Helmy, Mohammed Hesham Aboelenien

“…Bonferroni correction test was employed to evaluate a pairwise intervention for outcome variables that revealed there were insignificantly increases in vital capacity (VC), forced vital capacity (FVC), and maximal voluntary breathing (MVV) after the intervention compared to before intervention ( p > 0.05). …”
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Tumor Size Is a Critical Factor in Adjuvant Chemotherapy for T3-4aN0M0 Gastric Cancer Patients after D2 Gastrectomy by Shi Chen, Li-Ying Ou-Yang, Run-Cong Nie, Yuan-Fang Li, Jun Xiang, Zhi-Wei Zhou, Ying-Bo Chen, Jun-Sheng Peng

“…Chi-square tests and Kaplan-Meier methods were employed to compare the clinicopathological variables and prognoses. …”
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Vegetation greening and climate change respectively regulates the long-term trend and interannual variability in evapotranspiration over the Loess Plateau since the 21st century by Qiaoyin Tan, Ting Hua, Ting Hua, Haichen Zhao, Peiye Zhou, Peiye Zhou

“…Despite these findings, the underlying mechanisms driving ET variations remain contentious due to the complex interplay of multiple factors. In this study, we employed a logical attribution method, which attributes vegetation changes predominantly to anthropogenic activities (e.g., reforestation or land-use changes), while directly linking changes in climatic factors (e.g., temperature and precipitation) to climate change. …”
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Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study by Wenhao Cheng, Yinghui Li, Wei Yang, Siyang Wu, Mengmeng Wei, Yang Gao, Chen Kang, Shuofeng Zhang, Yingfei Li

“…A simple and sensitive UPLC-MS/MS bioanalytical method for the simultaneous determination of 13 ingredients of RPM, including chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, torachrysone-8-O-glucoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside, in rat plasma was established. Acetonitrile was employed to precipitate the plasma with appropriate sensitivity and acceptable matrix effects. …”
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The relationships between mental health and social media addiction, and between academic burnout and social media addiction among Chinese college students: A network analysis by Tingwei Feng, Buyao Wang, Mingdi Mi, Lei Ren, Lin Wu, Hui Wang, Xufeng Liu, Xiuchao Wang

“…Network analysis was performed using R to model the relationships between study variables, employing Gaussian Graphical Models and the least absolute shrinkage and selection operator technique for robust network estimation. …”
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MUC2 expression modulates immune infiltration in colorectal cancer by Christophe M. Raynaud, Ayesha Jabeen, Eiman I. Ahmed, Eiman I. Ahmed, Satanay Hubrack, Apryl Sanchez, Shimaa Sherif, Ahmad A. Al-Shaibi, Bernice Lo, Bernice Lo, Jessica Roelands, Davide Bedognetti, Davide Bedognetti, Wouter Hendrickx, Wouter Hendrickx

“…MUC2, a major component of the protective mucus layer in the gastrointestinal tract, has been implicated in CRC progression and immune response regulation.MethodIn this study, we sought to elucidate the relationship between MUC2 expression and immune infiltration within CRC using in vitro models involving two well-established cell lines, HT-29 and LS-174T. By employing CRISPR-mediated MUC2 knockout, we investigated the influence of MUC2 on tumor immune infiltration and its interplay with T cells and NK cells enriched peripheral blood mononuclear cells (PBMCs) in 3D spheroid cultures.ResultsWhile MUC2 was more abundant in LS-174T cell line compared to HT-29, its knockout resulted in increased immune infiltration solely in the HT-29 cell line, but not in the LS-174T cell line. …”
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New rectum dose surface mapping methodology to identify rectal subregions associated with toxicities following prostate cancer radiotherapy by Artemis Bouzaki, Dylan Green, Marcel van Herk, Jane Shortall, Tanuj Puri, Sarah Kerns, David Azria, Marrie-Pierre Farcy-Jacquet, Jenny Chang-Claude, Ananya Choudhury, Alison Dunning, Maarten Lambrecht, Barbara Avuzzi, Dirk De Ruysscher, Petra Seibold, Elena Sperk, Christopher Talbot, Ana Vega, Liv Veldeman, Adam Webb, Barry Rosenstein, Catharine M. West, Eliana Gioscio, Tiziana Rancati, Eliana Vasquez Osorio, Alan McWilliam

“…Existing methodologies employ a fixed, arbitrary physical extent for rectal dose mapping, limiting their analysis. …”
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